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公开(公告)号：US20110097445A1
公开(公告)日：2011-04-28
申请号：US12984826
申请日：2011-01-05
申请人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Søren Flensted Lassen , Peter Rahbek Østergaard
发明人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Søren Flensted Lassen , Peter Rahbek Østergaard
CPC分类号： C12N9/58 , A01K2217/05 , A23K20/189 , C12N9/48 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.
摘要翻译： 本发明涉及编码诺卡氏菌蛋白酶的新型3D结构，以及与诺卡氏菌蛋白酶同源的亲本蛋白酶的变体，优选具有改善的热稳定性和/或具有修改的温度活性分布。 本发明还涉及编码这些变体的DNA序列，其在重组宿主细胞中的产生，以及使用该变体，特别是在动物饲料和洗涤剂领域中的方法。 本发明还涉及生成和制备修饰性质的蛋白酶变体的方法。
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公开(公告)号：US20090047387A1
公开(公告)日：2009-02-19
申请号：US10574554
申请日：2004-10-08
申请人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Soren Flensted Lassen , Peter Rahbek Ostergaard
发明人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Soren Flensted Lassen , Peter Rahbek Ostergaard
IPC分类号： A23K1/165 , C12N9/50 , C12N15/11 , C12N15/00 , C12N1/20 , C11D3/386 , C12P21/04 , A01H5/00 , C07K1/00
CPC分类号： C12N9/58 , A01K2217/05 , A23K20/189 , C12N9/48 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.
摘要翻译： 本发明涉及编码诺卡氏菌蛋白酶的新型3D结构，以及与诺卡氏菌蛋白酶同源的亲本蛋白酶的变体，优选具有改善的热稳定性和/或具有修改的温度活性分布。 本发明还涉及编码这些变体的DNA序列，其在重组宿主细胞中的产生，以及使用该变体，特别是在动物饲料和洗涤剂领域中的方法。 本发明还涉及生成和制备修饰性质的蛋白酶变体的方法。
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公开(公告)号：US08377677B2
公开(公告)日：2013-02-19
申请号：US12984826
申请日：2011-01-05
申请人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Soren Flensted Lassen , Peter Rahbek Ostergaard
发明人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Soren Flensted Lassen , Peter Rahbek Ostergaard
CPC分类号： C12N9/58 , A01K2217/05 , A23K20/189 , C12N9/48 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.
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公开(公告)号：US07892808B2
公开(公告)日：2011-02-22
申请号：US10574554
申请日：2004-10-08
申请人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Soren Flensted Lassen , Peter Rahbek Ostergaard
发明人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Soren Flensted Lassen , Peter Rahbek Ostergaard
CPC分类号： C12N9/58 , A01K2217/05 , A23K20/189 , C12N9/48 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel 3D structure determined for a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an altered temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention further relates to methods of generating and preparing protease variants having different properties.
摘要翻译： 本发明涉及针对诺卡氏菌蛋白酶确定的新颖3D结构，以及与诺卡氏菌蛋白酶同源的亲本蛋白酶的变体，优选具有改善的热稳定性和/或具有改变的温度活性分布。 本发明还涉及编码这些变体的DNA序列，其在重组宿主细胞中的产生，以及使用该变体，特别是在动物饲料和洗涤剂领域中的方法。 本发明还涉及生成和制备具有不同性质的蛋白酶变体的方法。
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公开(公告)号：US20090203106A1
公开(公告)日：2009-08-13
申请号：US12420446
申请日：2009-04-08
申请人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Carsten Ryttergaard , Sine Larsen
发明人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Carsten Ryttergaard , Sine Larsen
IPC分类号： C12N9/24
CPC分类号： C12N9/2468 , C12Y302/01089
摘要： The present invention relates to variants of Glycoside Hydrolase family 53 galactanases, e.g., variants of the galactanases from strains of Yersinia, Aspergillus, Humicola, Meripilus, Myceliophthora, Thermomyces, Bacillus, Bifidobacterium, Cellvibrio, Clostridium, Pseudomonas, Thermotoga, or Xanthomonas.
摘要翻译： 本发明涉及糖苷水解酶家族53半乳聚糖酶的变体，例如来自耶尔森氏菌，曲霉属，腐质霉属，Meripilus，Myceliophthora，Thermomyces，Bacillus，Bifidobacterium，Cellvibrio，Clostridium，Pseudomonas，Thermotoga或黄单胞菌属的半乳聚糖酶的变体。
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公开(公告)号：US08367390B2
公开(公告)日：2013-02-05
申请号：US12420446
申请日：2009-04-08
申请人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Sine Larsen , Carsten Ryttersgaard
发明人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Sine Larsen , Carsten Ryttersgaard
CPC分类号： C12N9/2468 , C12Y302/01089
摘要： The present invention relates to variants of Glycoside Hydrolase family 53 galactanases, e.g., variants of the galactanases from strains of Yersinia, Aspergillus, Humicola, Meripilus, Myceliophthora, Thermomyces, Bacillus, Bifidobacterium, Cellvibrio, Clostridium, Pseudomonas, Thermotoga, or Xanthomonas.
摘要翻译： 本发明涉及糖苷水解酶家族53半乳聚糖酶的变体，例如来自耶尔森氏菌，曲霉属，腐质霉属，Meripilus，Myceliophthora，Thermomyces，Bacillus，Bifidobacterium，Cellvibrio，Clostridium，Pseudomonas，Thermotoga或黄单胞菌属的半乳聚糖酶的变体。
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公开(公告)号：US07537921B2
公开(公告)日：2009-05-26
申请号：US10537746
申请日：2003-12-11
申请人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Sine Larsen , Carsten Ryttersgaard
发明人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Sine Larsen , Carsten Ryttersgaard
CPC分类号： C12N9/2468 , C12Y302/01089
摘要： Variants of Glycoside Hydrolase family 53 galactanases, e.g. variants of the galactanases from strains of Yersinia, Aspergillus, Humicola, Meripilus, Myceliophthora, Thermomyces, Bacillus, Bifidobacterium, Cellvibrio, Clostridium, Pseudomonas, Thermotoga, or Xanthomonas.
摘要翻译： 糖苷类水解酶家族53半乳聚糖酶的变体，例如 来自耶尔森氏菌属，曲霉属，腐质霉属，Meripilus，Myceliophthora，Thermomyces，Bacillus，Bifidobacterium，Cellvibrio，Clostridium，Pseudomonas，Thermotoga或Xanthomonas的菌株的半乳聚糖酶的变体。
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公开(公告)号：US20110258714A1
公开(公告)日：2011-10-20
申请号：US12294526
申请日：2007-03-19
IPC分类号： A01K67/00 , C12N15/63 , C12N5/10 , C07H21/00 , A23L1/304 , A01H5/00 , A23K1/16 , A23K1/18 , A23L1/29 , A23L1/302 , C12N9/16 , C12N1/00
CPC分类号： C12N9/16 , A23K20/189 , C12Y301/03
摘要： The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
摘要翻译： 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如， 在动物饲料和动物饲料添加剂中。
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公开(公告)号：US08460656B2
公开(公告)日：2013-06-11
申请号：US12294526
申请日：2007-03-19
CPC分类号： C12N9/16 , A23K20/189 , C12Y301/03
摘要： The present invention relates to a phytase which has at least 80% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration when compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
摘要翻译： 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少80％的同一性，并且与该植酸酶相比包含至少一个变化。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如， 在动物饲料和动物饲料添加剂中。
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公开(公告)号：US08415119B2
公开(公告)日：2013-04-09
申请号：US12867612
申请日：2009-02-20
摘要： The present invention relates to a method of producing a protein of interest comprising: (a) cultivating a mutant cell under conditions conducive for production of the protein wherein the mutant has a reduced or no expression of an endogenous polypeptide shown in SEQ ID NO: 2 compared to a parent host cell grown under the same conditions; and optionally (b) recovering the protein of interest.
摘要翻译： 本发明涉及产生感兴趣的蛋白质的方法，包括：（a）在有助于产生蛋白质的条件下培养突变细胞，其中突变体具有SEQ ID NO：2所示的内源性多肽的减少或不表达 与在相同条件下生长的亲本宿主细胞相比; 和（b）回收目的蛋白质。
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