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18 条记录 (耗时 0.027 秒)

    Hybrid plasmid and process of making same
    5.
    发明授权
    Hybrid plasmid and process of making same 失效
    杂交质粒及其制备方法

    公开(公告)号：US4332898A

    公开(公告)日：1982-06-01

    申请号：US159852

    申请日：1980-06-16

    申请人： Fritz Reusser

    发明人： Fritz Reusser

    IPC分类号： C12N15/70 ,  C12N15/75 ,  C12N15/00

    CPC分类号： C12N15/70 ,  C12N15/75 ,  C12R1/19 ,  Y10S435/849

    摘要： A novel chemical compound, plasmid pUC1021, which was constructed from Bacillus megaterium chromosomal DNA and plasmid pBR322. Hybrid plasmid pUC1021 contains a functional tet gene promoter, and, thus, is useful as a cloning vehicle in recombinant DNA work. For example, using well known DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC1021 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

    摘要翻译： 一种新型化合物，质粒pUC1021，其由巨大芽孢杆菌染色体DNA和质粒pBR322构建。 杂交质粒pUC1021含有功能性的tet基因启动子，因此可用作重组DNA工作中的克隆载体。 例如，使用众所周知的DNA方法，可以将期望的基因，例如胰岛素基因插入到pUC1021中，然后将得到的质粒转化成合适的宿主微生物，培养后产生所需的胰岛素。

    Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli containing same
    6.
    发明授权
    Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli containing same 失效
    pBR322和Streptomyces质粒的杂交质粒和含有其的大肠杆菌

    公开(公告)号：US4343906A

    公开(公告)日：1982-08-10

    申请号：US068418

    申请日：1979-08-21

    申请人： Fritz Reusser ,  Vedpal S. Malik

    发明人： Fritz Reusser ,  Vedpal S. Malik

    IPC分类号： C12N15/76 ,  C12N1/20

    CPC分类号： C12N15/76

    摘要： A process for cloning DNA into a suitable host, which comprises fragmenting said DNA to obtain fragmented DNA, ligating said fragmented DNA into a suitable vector to obtain chimeric (hybrid) DNA, and transforming said chimeric DNA into said ultimate host. By this process, the useful chemical plasmid pUC3, which is obtainable from a biologically pure culture of the microorganism Streptomyces sp. 3022a, NRRL 11441, is cloned into the well-known bacterium E. coli HB101. This cloning of pUC3 into E. coli HB101 enables the production of large amounts of plasmid pUC3 DNA. pUC3 is useful as a cloning vehicle in recombinant DNA work. For example, using recombinant DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC3 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

    摘要翻译： 将DNA克隆到合适的宿主中的方法，其包括将所述DNA片段化以获得片段化的DNA，将所述片段化DNA连接到合适的载体中以获得嵌合（杂交）DNA，并将所述嵌合DNA转化到所述最终宿主中。 通过这个过程，可以从微生物链霉菌Streptomyces sp。的生物纯培养物获得的有用的化学质粒pUC3。 3022a，NRRL 11441克隆到公知的大肠杆菌HB101中。 将pUC3克隆到大肠杆菌HB101中能够产生大量的质粒pUC3DNA。 pUC3可用作重组DNA工作中的克隆载体。 例如，使用重组DNA方法，将期望的基因，例如胰岛素基因插入到pUC3中，然后将得到的质粒转化成合适的宿主微生物，培养后产生所需的胰岛素。

    Hybrid plasmid and process of making same
    9.
    发明授权
    Hybrid plasmid and process of making same 失效
    杂交质粒及其制备方法

    公开(公告)号：US4362817A

    公开(公告)日：1982-12-07

    申请号：US239258

    申请日：1981-03-02

    申请人： Fritz Reusser

    发明人： Fritz Reusser

    IPC分类号： C12N15/70 ,  C12N15/76 ,  C12N15/00 ,  C12N1/00 ,  C12N1/20 ,  C12R1/19

    CPC分类号： C12N15/76 ,  C12N15/70 ,  Y10S435/849

    摘要： A novel chemical compound, plasmid pUC1060, which was constructed from Streptomyces sp. 3022a chromosomal DNA and plasmid pBR322. Hybrid plasmid pUC1060 contains a functional tet gene promoter composed of streptomycete and E. coli DNA, and, thus, is useful as a cloning vehicle in recombinant DNA work. For example, using well known DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC1060 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

    摘要翻译： 一种新型化合物，质粒pUC1060，由Streptomyces sp。 3022a染色体DNA和质粒pBR322。 杂交质粒pUC1060含有由链霉菌和大肠杆菌DNA组成的功能性tet基因启动子，因此可用作重组DNA工作中的克隆载体。 例如，使用众所周知的DNA方法，可将所需基因，例如胰岛素基因插入到pUC1060中，然后将得到的质粒转化成合适的宿主微生物，培养后产生所需的胰岛素。

    Process for producing antibiotic U-51,640
    10.
    发明授权
    Process for producing antibiotic U-51,640 失效
    生产抗生素U-51,640的方法

    公开(公告)号：US3998698A

    公开(公告)日：1976-12-21

    申请号：US674860

    申请日：1976-04-08

    申请人： Alexander D. Argoudelis ,  Fritz Reusser

    发明人： Alexander D. Argoudelis ,  Fritz Reusser

    IPC分类号： C07H5/04 ,  C12D9/00

    CPC分类号： C07H5/04 ,  Y10S435/886

    摘要： Antibiotic U-51,640 produced by the controlled fermentation of the microorganism Streptomyces ficellus Dietz, sp. n., NRRL 8067. This antibiotic and its acid addition salts are active against Gram-positive and Gram-negative bacteria. Accordingly, they can be used in various environments to eradicate or control such bacteria.

    摘要翻译： 通过微生物链霉菌（Streptomyces ficellus Dietz）的受控发酵产生的抗生素U-51,640。 这种抗生素及其酸加成盐对革兰氏阳性和革兰氏阴性菌具有活性。 因此，它们可以用于各种环境中以消除或控制这些细菌。