公开(公告)号：US6107092A

公开(公告)日：2000-08-22

申请号：US280409

申请日：1999-03-29

申请人： Lex M. Cowsert ,  C. Frank Bennett ,  Bert W. O'Malley

发明人： Lex M. Cowsert ,  C. Frank Bennett ,  Bert W. O'Malley

IPC分类号： A61K38/00 ,  C12N15/113 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68

CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  Y02P20/582

摘要： Antisense compounds, compositions and methods are provided for modulating the function or amount of SRA. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to SRA or nucleic acids encoding SRA. Methods of using these compounds for modulation of SRA levels and for treatment of diseases associated with SRA are provided.

摘要翻译： 提供反义化合物，组合物和方法用于调节SRA的功能或量。 组合物包含靶向SRA或编码SRA的核酸的反义化合物，特别是反义寡核苷酸。 提供了使用这些化合物调节SRA水平和治疗与SRA相关疾病的方法。

公开(公告)号：US5945279A

公开(公告)日：1999-08-31

申请号：US459067

申请日：1995-06-02

申请人： Bert W. O'Malley ,  Orla M. Conneely ,  Ronan F. Power

发明人： Bert W. O'Malley ,  Orla M. Conneely ,  Ronan F. Power

IPC分类号： C07K14/705 ,  C12N15/10 ,  C12N15/12 ,  G01N33/566 ,  G01N33/94 ,  C12Q1/68 ,  C12N15/85

CPC分类号： C07K14/705 ,  C12N15/10 ,  G01N33/566 ,  G01N33/9413 ,  G01N2333/723 ,  G01N2500/02 ,  Y10S435/975

摘要： A tissue culture screening system to monitor a transcriptional response treated by a chemical signal interacting with a plasma membrane receptor is provided. The tissue culture screening system includes a cell line containing a membrane receptor, a target gene and a specific receptor selected from the group consisting of a steroid receptor, a vitamin receptor and an orphan receptor. The specific receptor regulates transcription of the target gene. Any of the target gene membrane receptor or specific receptor can be introduced into the cell by an expression vector. In addition to the screening system there is also provided assays for identifying test compounds and chemical signals that regulate transcription or are potential agonist or antagonist neurotransmitters or which regulate indirectly by a membrane receptor binding or regulate transription in the absence of a steroid, vitamin or orphan ligand. There is further provided kits for the assays.

摘要翻译： 提供了一种组织培养筛选系统，用于监测由与质膜受体相互作用的化学信号处理的转录反应。 组织培养筛选系统包括含有受体的细胞系，靶基因和选自类固醇受体，维生素受体和孤儿受体的特异性受体。 特异性受体调节靶基因的转录。 可以通过表达载体将任何靶基因膜受体或特异性受体引入细胞。 除了筛选系统之外，还提供用于鉴定测试化合物和调节转录的化学信号的测定法，或者是潜在的激动剂或拮抗剂神经递质或通过膜受体结合或调节在不存在类固醇，维生素或孤儿的情况下间接调控 配体。 还有用于测定的试剂盒。

公开(公告)号：US5935934A

公开(公告)日：1999-08-10

申请号：US454418

申请日：1995-05-30

申请人： Elisabetta Vegeto ,  Donald P. McDonnell ,  Bert W. O'Malley ,  William T. Schrader ,  Ming-Jer Tsai

发明人： Elisabetta Vegeto ,  Donald P. McDonnell ,  Bert W. O'Malley ,  William T. Schrader ,  Ming-Jer Tsai

IPC分类号： A61K31/70 ,  A61K38/00 ,  A61K48/00 ,  C07H15/12 ,  C07H17/00 ,  C07H21/04 ,  C07K14/705 ,  C07K14/72 ,  C12N5/00 ,  C12N5/10 ,  C12N7/01 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/82 ,  C12P21/02 ,  C12R1/91 ,  G01N33/566 ,  G01N33/68 ,  G01N33/74

CPC分类号： C07K14/721 ,  C12N15/63 ,  C12N15/8237 ,  C12N15/8238 ,  G01N33/566 ,  G01N33/743 ,  A61K38/00 ,  G01N2333/723 ,  G01N2500/10 ,  Y10S514/01

摘要： The present invention provides mutant proteins of steroid hormone receptors. These mutant proteins are useful in methods of distinguishing a steroid hormone receptor antagonist from a steroid hormone receptor agonist. The present invention also provides plasmids containing mutated steroid hormone receptor proteins and cells transfected with those plasmids. In addition, the present invention provides methods for determining whether a compound is a steroid hormone receptor antagonist or agonist. Also, the present invention provides methods of determining endogenous ligands for steroid hormone receptors. The invention further provides a molecular switch for regulating expression in gene therapy and methods of employing the molecular switch in humans, animals, transgenic animals and plants.

公开(公告)号：US5759785A

公开(公告)日：1998-06-02

申请号：US448270

申请日：1995-05-23

申请人： Ming-Jer Tsai ,  Bert W. O'Malley ,  Sophia Yang Tsai ,  George Francis Allan

发明人： Ming-Jer Tsai ,  Bert W. O'Malley ,  Sophia Yang Tsai ,  George Francis Allan

IPC分类号： G01N33/74 ,  G01N33/53

CPC分类号： G01N33/743 ,  Y10S436/817 ,  Y10T436/25 ,  Y10T436/25125

摘要： The present invention provides a novel method of determining the antagonist and agonist activity of a compound for steroid hormone receptors. The present invention also provides a method of determining antagonist activity of a compound for a hormone receptor by inducing the conformational change in the receptor. In addition, the present invention provides a novel method of determining the level of agonist activity of a compound for steroid hormone receptors.

摘要翻译： 本发明提供了一种测定类固醇激素受体化合物的拮抗剂和激动剂活性的新方法。 本发明还提供了通过诱导受体的构象变化来测定激素受体化合物的拮抗剂活性的方法。 此外，本发明提供了确定类固醇激素受体化合物的激动剂活性水平的新方法。

公开(公告)号：US5571697A

公开(公告)日：1996-11-05

申请号：US303009

申请日：1994-11-02

申请人： Orla M. Conneely ,  Denis R. Headon ,  Bert W. O'Malley

发明人： Orla M. Conneely ,  Denis R. Headon ,  Bert W. O'Malley

IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/79 ,  C12N1/15 ,  C12N15/12 ,  C12N15/80 ,  C12P21/02 ,  C12R1/665 ,  C12R1/69 ,  C12N15/62

CPC分类号： C12N15/80 ,  C07K14/79 ,  A61K38/00 ,  C07K2319/02

摘要： The subject invention provides for the production of lactoferrins and lactoferrin polypeptide fragments using the host cells Aspergillus in combination with novel plasmid constructs. More specifically, the subject invention provides novel vector constructs capable of producing lactoferrins and lactoferrin polypeptide fragments in Aspergillus host cells. More particularly, the subject invention provides for novel plasmid constructs suitable for use with Aspergillus and especially Aspergillus awamori, niger and oryzae host cells, which enables them to produce large amounts of recombinant lactoferrins and lactoferrin polypeptide fragments.

公开(公告)号：US06416998B1

公开(公告)日：2002-07-09

申请号：US08479913

申请日：1995-06-07

申请人： Bert W. O'Malley ,  Ming-Jer Tsai ,  Harry C. Ledebur, Jr. ,  Joseph D. Kittle, Jr.

发明人： Bert W. O'Malley ,  Ming-Jer Tsai ,  Harry C. Ledebur, Jr. ,  Joseph D. Kittle, Jr.

IPC分类号： C12N1512

CPC分类号： C12N15/8237 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/721 ,  C12N15/63 ,  C12N15/8238 ,  G01N33/566 ,  G01N33/743 ,  G01N2333/723 ,  G01N2500/10

摘要： The present invention provides mutated proteins of steroid hormone receptors. These mutated proteins are useful as gene medicines. In particular, these mutated proteins are useful for regulating expression of genes in gene therapy. In addition, the present invention provides plasmids encoding for the desired mutated steroid hormone receptor proteins, as well as cells transfected with those plasmids.

摘要翻译： 本发明提供类固醇激素受体的突变蛋白。 这些突变的蛋白质可用作基因药物。 特别地，这些突变的蛋白质可用于调节基因治疗中基因的表达。 此外，本发明提供编码所需突变的类固醇激素受体蛋白质的质粒以及用这些质粒转染的细胞。

公开(公告)号：US06228614B1

公开(公告)日：2001-05-08

申请号：US09265577

申请日：1999-03-09

申请人： Orla M. Conneely ,  Denis R. Headon ,  Bert W. O'Malley ,  Gregory S. May

发明人： Orla M. Conneely ,  Denis R. Headon ,  Bert W. O'Malley ,  Gregory S. May

IPC分类号： C12N1509

CPC分类号： C07K14/79

摘要： The verified cDNA sequences for human, bovine and porcine lactoferrin protein have been used to prepare recombinant lactoferrin for therapeutic and nutritional applications. Regions of the cDNA such as the Fe binding sites can be used to make an hLF polypeptide product. The present invention provides novel plasmids, transfected eucaryotic cells and methods of producing these plasmids and transfected eucaryotic cells. The novel plasmid contains the cDNA for lactoferrin protein. Methods for the production of lactoferrin protein in fungi and bacteria are also provided. Thus, the present invention provides an efficient and economical means for the production of recombinant lactoferrin protein and lactoferrin related polypeptides.

摘要翻译： 用于人，牛和猪乳铁蛋白的经证实的cDNA序列已被用于制备用于治疗和营养应用的重组乳铁蛋白。 可以使用诸如Fe结合位点的cDNA的区域来制备hLF多肽产物。本发明提供了新的质粒，转染的真核细胞以及产生这些质粒和转染的真核细胞的方法。 新型质粒含有乳铁蛋白的cDNA。 还提供了在真菌和细菌中生产乳铁蛋白的方法。 因此，本发明提供了用于生产重组乳铁蛋白和乳铁蛋白相关多肽的有效和经济的手段。

公开(公告)号：US5955316A

公开(公告)日：1999-09-21

申请号：US691123

申请日：1996-08-01

申请人： Orla M. Conneely ,  Denis R. Headon ,  Bert W. O'Malley

发明人： Orla M. Conneely ,  Denis R. Headon ,  Bert W. O'Malley

IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/79 ,  C12N1/15 ,  C12N15/12 ,  C12N15/80 ,  C12P21/02 ,  C12R1/665 ,  C12R1/69 ,  C12N15/62 ,  C12N15/64

CPC分类号： C12N15/80 ,  C07K14/79 ,  A61K38/00 ,  C07K2319/02

摘要： The subject invention provides for the production of lactoferrins and lactoferrin polypeptide fragments using the host cells Aspergillus in combination with novel plasmid constructs. More specifically, the subject invention provides novel vector constructs capable of producing lactoferrins and lactoferrin polypeptide fragments in Aspergillus host cells. More particularly, the subject invention provides for novel plasmid constructs suitable for use with Aspergillus and especially Aspergillus awamori, niger and oryzae host cells, which enables them to produce large amounts of recombinant lactoferrins and lactoferrin polypeptide fragments.

摘要翻译： 本发明提供使用宿主细胞曲霉与新型质粒构建体组合产生乳铁蛋白和乳铁蛋白多肽片段。 更具体地，本发明提供能够在曲霉属宿主细胞中产生乳铁蛋白和乳铁蛋白多肽片段的新型载体构建体。 更具体地，本发明提供了适用于曲霉属，特别是泡盛曲霉，黑曲霉和谷氨酸宿主细胞的新型质粒构建体，其使得它们能够产生大量的重组乳铁蛋白和乳铁蛋白多肽片段。

公开(公告)号：US5925564A

公开(公告)日：1999-07-20

申请号：US472809

申请日：1995-06-07

申请人： Robert J. Schwartz ,  Franco J. DeMayo ,  Bert W. O'Malley

发明人： Robert J. Schwartz ,  Franco J. DeMayo ,  Bert W. O'Malley

IPC分类号： A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  C07K14/47 ,  C12N15/85 ,  C12N5/16 ,  C12N15/63

CPC分类号： A61K48/00 ,  C07K14/4708 ,  C12N15/85 ,  A61K38/00 ,  A61K39/00 ,  C12N2830/008 ,  C12N2830/90

摘要： This invention relates to gene therapy by using vectors which encode stable MRNA and methods of using such vectors. In particular, this invention relates to vectors which establish controlled expression of recombinant genes within tissues at certain levels. The vector includes a 5' flanking region which includes necessary sequences for expression of a nucleic acid cassette, a 3' flanking region including a 3' UTR and/or 3' NCR which stabilizes mRNA expressed from the nucleic acid cassette, and a linker which connects the 5' flanking region to a nucleic acid sequence. The linker has a position for inserting a nucleic acid cassette. The linker does not contain the coding sequence of a gene that the linker is naturally associated with. The 3' flanking region is 3' to the position for inserting the nucleic acid cassette. The expression vectors of the present invention can also be regulated by a regulatory system and/or constructed with a coating.

摘要翻译： 本发明涉及通过使用编码稳定MRNA的载体和使用这些载体的方法进行基因治疗。 特别地，本发明涉及在某些水平的组织内建立重组基因的受控表达的载体。 载体包括5'侧翼区，其包含用于表达核酸盒的必需序列，包含稳定从核酸盒表达的mRNA的3'UTR和/或3'NCR的3'侧翼区和连接体 将5'侧翼区连接到核酸序列。 接头具有用于插入核酸盒的位置。 接头不包含接头天然相关基因的编码序列。 3'侧翼区域为插入核酸盒的位置3'。 本发明的表达载体也可以通过调节系统调节和/或用涂层构建。

公开(公告)号：US5849881A

公开(公告)日：1998-12-15

申请号：US456106

申请日：1995-05-30

申请人： Orla M. Conneely ,  Denis R. Headon ,  Bert W. O'Malley ,  Gregory S. May

发明人： Orla M. Conneely ,  Denis R. Headon ,  Bert W. O'Malley ,  Gregory S. May

IPC分类号： A61K38/00 ,  C07K14/79 ,  C12N15/80 ,  C07K14/435 ,  C12N15/81

CPC分类号： C07K14/79 ,  C12N15/80 ,  A61K38/00

摘要： The verified cDNA sequences for human, bovine and porcine lactoferrin protein have been used to prepare recombinant lactoferrin for therapeutic and nutritional applications. Regions of the cDNA such as the Fe binding sites can be used to make an hLF polypeptide product. The present invention provides novel plasmids, transfected eucaryotic cells and methods of producing these plasmids and transfected eucaryotic cells. The novel plasmid contains the cDNA for lactoferrin protein. Methods for the production of lactoferrin protein in fungi and bacteria are also provided. Thus, the present invention provides an efficient and economical means for the production of recombinant lactoferrin protein and lactoferrin related polypeptides.

摘要翻译： 用于人，牛和猪乳铁蛋白的经证实的cDNA序列已被用于制备用于治疗和营养应用的重组乳铁蛋白。 诸如Fe结合位点的cDNA区域可用于制备hLF多肽产物。 本发明提供新颖的质粒，转染的真核细胞以及生产这些质粒和转染的真核细胞的方法。 新型质粒含有乳铁蛋白的cDNA。 还提供了在真菌和细菌中生产乳铁蛋白的方法。 因此，本发明提供了用于生产重组乳铁蛋白和乳铁蛋白相关多肽的有效和经济的手段。