公开(公告)号：US20240263163A1

公开(公告)日：2024-08-08

申请号：US18565957

申请日：2022-06-01

Applicant: McMaster University

Inventor： Tamaghna Gupta ,  Rakesh P. Sahu ,  Ishwar K. Puri

IPC: C12N13/00 ,  C12M1/32 ,  C12M1/42 ,  C12N5/071

CPC classification number: C12N13/00 ,  C12M23/12 ,  C12M35/06 ,  C12N5/0688 ,  C12N2529/00 ,  C12N2533/90

Abstract: This application relates to methods of generating cell/particle-free regions in a cellular/particle aggregate using label-free magnetic manipulation. For example, the method may include suspending cells/particles in a paramagnetic culture medium, the cells/particles being diamagnetic; seeding the cells/particles and the paramagnetic culture medium into microwells; and placing the microwells over an array of magnet pairs where each magnet pair creates a magnetic field gradient. The magnetic susceptibility difference and the magnetic field gradient drive the cells/particles towards a spaced region between magnets in the magnet pair having a lowest magnetic field strength to create a cell/particle aggregate with a cell-free/particle-free area where the cell/particle aggregate surrounds the cell-free/particle-free area.

公开(公告)号：US11788057B2

公开(公告)日：2023-10-17

申请号：US16178172

申请日：2018-11-01

Applicant: McMaster University

Inventor： Abdel Rahman Abdel Fattah ,  Rakesh Prasad Sahu ,  Fei Geng ,  Sarah Mishriki ,  Elvira Meleca ,  Ishwar K. Puri ,  Suvojit Ghosh

IPC: C12N5/00 ,  G01N33/50 ,  C12Q1/6876

CPC classification number: C12N5/0062 ,  C12Q1/6876 ,  G01N33/5032 ,  G01N33/5082 ,  C12N2513/00 ,  C12N2527/00 ,  C12Q2600/136

Abstract: A novel macroscale, contactless, label-free method to print in situ three-dimensional (3D) particle assemblies of different morphologies and sizes is demonstrated using non-adherent (blood) and adherent (MCF-7 and HUVEC) cells. This method of manipulating particles such as cells or biological moleules does not necessarily require the use of nozzles that can contaminate the cell suspension, or to which cells can adhere. Instead, the intrinsic diamagnetic properties of particles such as cells are used to magnetically manipulate them in situ in a nontoxic paramagnetic medium, creating various shapes such as (a) rectangular bar, (b) three-pointed star, and (c) spheroids of varying sizes. A normal distribution of 3D cell structures is produced when formed through magnetic assembly. The use of this method in co-culturing of different cell lines is also demonstrated. The technique is envisioned to be transferable to other cell lines or diamagnetic biological molecules, with potential applications in tissue engineering, medical diagnostics and drug screening.

公开(公告)号：US20190200488A1

公开(公告)日：2019-06-27

申请号：US16229219

申请日：2018-12-21

Applicant: MCMASTER UNIVERSITY

Inventor： Souvik Pal ,  Hosein Moazami-goodarzi ,  Suvojit Ghosh ,  Kathryn Thorn ,  Ishwar K. Puri

IPC: H05K7/20

CPC classification number: H05K7/20736 ,  H05K7/20781 ,  H05K7/20836

Abstract: A plate-fin heat exchanger has a gas coolant flow path whose length is at least twice its thickness, with thickness measured substantially transverse to the gas coolant flow path and substantially transverse to the liquid coolant flow path. The heat exchanger is well suited (although not limited) to use in cooling units for information technology equipment, and can provide substantial cooling while fitting within an enclosure having the same external form factors as standardized rack-mountable ITE configured to fit into standard IT racks. Thus, a cooling unit incorporating the heat exchanger can be installed just as a rack-mountable server is installed. The heat exchanger can fit within an enclosure whose height, measured substantially parallel to the thickness of the heat exchanger and hence substantially transverse to the gas coolant flow path and substantially transverse to the liquid coolant flow path, is a positive integral multiple of RU (1.75 inches).

公开(公告)号：US20180059100A1

公开(公告)日：2018-03-01

申请号：US15692006

申请日：2017-08-31

Applicant: McMaster University

Inventor： Abdel Rahman Abdel Fattah ,  Ahmed M. Abdalla ,  Fei Geng ,  Sarah Mishriki ,  Elvira Meleca ,  Ishwar K. Puri ,  Suvojit Ghosh

IPC: G01N33/543

CPC classification number: G01N33/5438 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54346 ,  G01N2333/4703

Abstract: A new magnetic carbon nanotube (mCNT) biosensor ink (mbio-ink) that utilizes immobilized capture agents to detect specific target analytes via a simple current response method is presented here, expanding the applications of magnetic carbon nanotubes as biomolecule-sensing nanomaterials. The employment of a magnetic field to print the mbio-ink into electrically conductive networks facilitates its integration with an external circuit via the use of a simple electrode system. When the sensor is connected to an external power source, the reduction in current, caused by an interaction between the capture agents and target analyte, is detected. The rapid detection technique and generic benchtop fabrication method allows for scale up, while the small volumes required and magnet independent electrical measurements renders the mbio-ink attractive for drug screening and disease detection applications.

公开(公告)号：US20190127685A1

公开(公告)日：2019-05-02

申请号：US16178172

申请日：2018-11-01

Applicant: McMaster University

Inventor： Abdel Rahman Abdel Fattah ,  Rakesh Prasad Sahu ,  Fei Geng ,  Sarah Mishriki ,  Elvira Meleca ,  Ishwar K. Puri ,  Suvojit Ghosh

IPC: C12N5/00 ,  G01N33/50 ,  C12Q1/6876

Abstract: A novel macroscale, contactless, label-free method to print in situ three-dimensional (3D) particle assemblies of different morphologies and sizes is demonstrated using non-adherent (blood) and adherent (MCF-7 and HUVEC) cells. This method of manipulating particles such as cells or biological moleules does not necessarily require the use of nozzles that can contaminate the cell suspension, or to which cells can adhere. Instead, the intrinsic diamagnetic properties of particles such as cells are used to magnetically manipulate them in situ in a nontoxic paramagnetic medium, creating various shapes such as (a) rectangular bar, (b) three-pointed star, and (c) spheroids of varying sizes. A normal distribution of 3D cell structures is produced when formed through magnetic assembly. The use of this method in co-culturing of different cell lines is also demonstrated. The technique is envisioned to be transferable to other cell lines or diamagnetic biological molecules, with potential applications in tissue engineering, medical diagnostics and drug screening.