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    Luminescence sensor operating in reflection mode
    1.
    发明授权
    Luminescence sensor operating in reflection mode 有权
    发光传感器以反射模式工作

    公开(公告)号：US08039814B2

    公开(公告)日：2011-10-18

    申请号：US12158451

    申请日：2006-12-19

    申请人： Maarten Marinus Johannes Wilhelm Van Herpen ,  Derk Jan Wilfred Klunder ,  Hendrik Roelof Stapert

    发明人： Maarten Marinus Johannes Wilhelm Van Herpen ,  Derk Jan Wilfred Klunder ,  Hendrik Roelof Stapert

    IPC分类号： G01N21/64

    CPC分类号： G01N21/648 ,  G01N21/6428

    摘要： A luminescence sensor, such as a luminescence biosensor or a luminescence chemical sensor, includes a substrate having at least one aperture filled with a medium. The aperture has a first lateral dimension larger than the diffraction limit of excitation radiation in the medium, and a second lateral dimension smaller than the diffraction limit of the excitation radiation in the medium. A method is also provided for the detection of luminescence radiation, e.g. fluorescence radiation, generated by at least one optically variable molecule, e.g. fluorophore, in the at least one aperture. Beneficially, the excitation radiation is polarized to suppress the excitation radiation in the apertures. The luminescence sensor and the method are able to detect relatively low concentrations of optically variable molecules, e.g. fluorophores.

    摘要翻译： 诸如发光生物传感器或发光化学传感器的发光传感器包括具有填充有介质的至少一个孔的基板。 该孔具有比介质中的激发辐射的衍射极限大的第一横向尺寸，以及小于介质中的激发辐射的衍射极限的第二横向尺寸。 还提供了一种用于检测发光辐射的方法，例如， 由至少一个光学可变分子产生的荧光辐射，例如。 荧光团，在至少一个孔中。 有利地，激发辐射被极化以抑制孔中的激发辐射。 发光传感器和方法能够检测相对低浓度的光学可变分子，例如， 荧光团

    Luminescence sensor using multi-layer substrate structure
    3.
    发明授权
    Luminescence sensor using multi-layer substrate structure 有权
    发光传感器采用多层衬底结构

    公开(公告)号：US07560708B2

    公开(公告)日：2009-07-14

    申请号：US11995696

    申请日：2006-07-07

    申请人： Maarten Marinus Johannes Wilhelm Van Herpen ,  Derk Jan Wilfred Klunder

    发明人： Maarten Marinus Johannes Wilhelm Van Herpen ,  Derk Jan Wilfred Klunder

    IPC分类号： G01J1/58

    CPC分类号： G01N21/76 ,  G01N21/648 ,  G01N33/54306 ,  G01N33/582

    摘要： The present invention provides a luminescence sensor (2), such as e.g. a luminescence biosensor, comprising a multi-layer structure. The multi-layer structure comprises at least a first layer (2a) formed of a first material and a second layer (2b) formed of a second material. The first material has a first binding capacity towards luminophores and the second material has a second binding capacity towards luminophores, the first binding capacity being different from the second binding capacity. The luminescence sensor (2) according to the present invention shows a high sensitivity because it provides preferred binding sites for luminophores at locations where the combined excitation and detection efficiency is the highest.

    摘要翻译： 本发明提供一种发光传感器（2），例如， 发光生物传感器，包括多层结构。 多层结构至少包括由第一材料形成的第一层（2a）和由第二材料形成的第二层（2b）。 第一材料具有朝向发光体的第一结合能力，第二材料具有朝向发光体的第二结合能力，第一结合能力不同于第二结合能力。 根据本发明的发光传感器（2）显示出高灵敏度，因为它在组合的激发和检测效率最高的位置处为发光体提供优选的结合位点。

    DETECTING TARGET MOLECULES IN A SAMPLE
    5.
    发明申请
    DETECTING TARGET MOLECULES IN A SAMPLE 审中-公开
    在样品中检测目标分子

    公开(公告)号：US20100197515A1

    公开(公告)日：2010-08-05

    申请号：US12446461

    申请日：2007-10-19

    申请人： Maarten Marinus Johannes Wilhelm Van Herpen ,  Derk Jan Wilfred Klunder ,  Hendrik Roelof Stapert

    发明人： Maarten Marinus Johannes Wilhelm Van Herpen ,  Derk Jan Wilfred Klunder ,  Hendrik Roelof Stapert

    IPC分类号： C40B30/04 ,  C40B40/04

    CPC分类号： G01N33/5306 ,  C40B30/10 ,  G01N21/6428

    摘要： The invention relates to detection the presence of a target molecule in a sample, wherein the sample is contacted with a substrate, the substrate subsequently being washed in a wash step. In particular, the invention relates to a method of detecting the presence of a target molecule in a sample, the method comprising: (a) contacting the sample (37) with a substrate having immobilized thereon probe molecules that specifically binds to the target molecule; (b) washing the substrate (38) in a wash step by a wash fluid in order to remove or dilute unbound target molecules; (c) detect the presence of resultant binding complexes (39) on the substrate to determine whether the target molecule is present in the sample. The wash fluid being substantially refractive index matched to the substrate.

    摘要翻译： 本发明涉及检测样品中靶分子的存在，其中样品与底物接触，随后在洗涤步骤中洗涤底物。 特别地，本发明涉及检测样品中目标分子的存在的方法，该方法包括：（a）使样品（37）与其上固定有特异性结合靶分子的探针分子的底物接触; （b）通过洗涤液在洗涤步骤中洗涤基质（38）以去除或稀释未结合的靶分子; （c）检测所得结合复合物（39）在底物上的存在，以确定靶分子是否存在于样品中。 洗涤液体基本上与基材的折射率匹配。

    RADIATION DETECTORS USING EVANESCENT FIELD EXCITATION
    6.
    发明申请
    RADIATION DETECTORS USING EVANESCENT FIELD EXCITATION 审中-公开
    使用现场激励的辐射探测器

    公开(公告)号：US20090284746A1

    公开(公告)日：2009-11-19

    申请号：US12373995

    申请日：2007-07-04

    申请人： Derk Jan Wilfred Klunder ,  Maarten Marinus Johannes Wilhelm Van Herpen ,  Marcello Leonardo Mario Balistreri ,  Marc Wilhelmus Gijsbert Ponjee ,  Mark Thomas Johnson

    发明人： Derk Jan Wilfred Klunder ,  Maarten Marinus Johannes Wilhelm Van Herpen ,  Marcello Leonardo Mario Balistreri ,  Marc Wilhelmus Gijsbert Ponjee ,  Mark Thomas Johnson

    IPC分类号： G01N21/55 ,  G01N21/62

    CPC分类号： G01N21/6454 ,  B01L3/5027 ,  G01N21/6428 ,  G01N21/648 ,  G01N2021/7786

    摘要： A detection system (100, 150, 180, 200, 220, 250) for detecting luminescence from at least one sample (108) when excited by incident excitation radiation. Detecting luminescence may allow to detect, for example, biological, chemical or bio-chemical particles. The detection system (100, 150, 180, 200, 220, 250) comprising at least one optical component (102) with at least a first surface (104). The first surface (104) of the at least one optical component (102) is located to internally reflect incident excitation radiation to create an evanescent field outside the at least one optical component (102) for exciting the at least one sample (108). The detection system also comprises at least one detector element (110) that is in direct contact with the at least one optical component (102) to detect the luminescence from at least one excited sample (108) through the at least one optical component (102).

    摘要翻译： 用于当由入射激发辐射激发时用于检测来自至少一个样品（108）的发光的检测系统（100,150,180,200,250,250）。 检测发光可以允许检测例如生物，化学或生物化学颗粒。 所述检测系统（100,150,180,200,250,250）包括具有至少第一表面（104）的至少一个光学部件（102）。 所述至少一个光学部件（102）的第一表面（104）位于内部反射入射的激发辐射，以在所述至少一个光学部件（102）外部产生消逝场，用于激发所述至少一个样品（108）。 检测系统还包括至少一个与至少一个光学部件（102）直接接触的检测器元件（110），以检测来自至少一个激发的样品（108）的至少一个光学部件（102）的发光 ）。