-
公开(公告)号：US20110081680A1
公开(公告)日：2011-04-07
申请号：US12965111
申请日：2010-12-10
IPC分类号： C12P21/00
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
摘要翻译： 本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。
-
2.发明授权公开(公告)号：US07094574B2
公开(公告)日：2006-08-22
申请号：US11123362
申请日：2005-05-06
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
-
公开(公告)号：US5854403A
公开(公告)日：1998-12-29
申请号：US653298
申请日：1996-05-24
申请人： Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
发明人： Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
IPC分类号： C12N15/09 , A61K35/14 , A61K38/00 , A61K38/43 , A61P7/04 , C07K1/18 , C07K1/22 , C07K14/47 , C07K14/755 , C12P21/00 , C07K1/14
CPC分类号： C07K14/755 , A61K38/00
摘要： The invention relates to a method for isolation of highly pure von Willebrand Factor in which recombinant von Willebrand Factor (rvWF) is chromatographically purified by anion exchange chromatography on an anion exchanger of the quaternary amino type in a buffer solution comprising buffer substances and optionally salt. The buffer solutions are preferably free of stabilizers, amino acids and other additives. According to this method, highly pure recombinant rvWF can be obtained, which is free from blood plasma proteins, especially free from Factor VIII, and is physiologically active. Further, the invention relates to a pharmaceutical preparation that contains rvWF, which comprises mulitimers with a high structural integrity.
摘要翻译： 本发明涉及一种用于分离高纯度血管性血友病因子的方法，其中重组血管性血友病因子（rvWF）通过阴离子交换色谱在季氨基型阴离子交换剂上在包含缓冲物质和任选盐的缓冲溶液中进行色谱纯化。 缓冲溶液优选不含稳定剂，氨基酸和其它添加剂。 根据该方法，可以获得高纯度的重组rvWF，其不含血浆蛋白质，特别是不含因子VIII，且具有生理活性。 此外，本发明涉及含有rvWF的药物制剂，其包含具有高结构完整性的多组分。
-
4.发明授权Recombinant cell clones having increased stability and methods of making and using the same 有权具有增加的稳定性的重组细胞克隆及其制备和使用方法公开(公告)号：US08084252B2
公开(公告)日：2011-12-27
申请号：US12848897
申请日：2010-08-02
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
摘要翻译： 公开了稳定的重组细胞克隆，其在无血清和无蛋白质的培养基中稳定至少40代，通过在无血清和无蛋白质培养条件下倍增稳定细胞克隆获得的生物质，以及制备重组蛋白质的方法 通过生物量。 此外，本发明涉及一种回收稳定的重组细胞克隆的方法。
-
5.发明申请RECOMBINANT CELL CLONES HAVING INCREASED STABILITY AND METHODS OF MAKING AND USING THE SAME 有权具有增加的稳定性的重组细胞克隆及其制备和使用方法公开(公告)号：US20110104758A1
公开(公告)日：2011-05-05
申请号：US12986111
申请日：2011-01-06
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
摘要翻译： 公开了稳定的重组细胞克隆，其在无血清和无蛋白质的培养基中稳定至少40代，通过在无血清和无蛋白质培养条件下倍增稳定细胞克隆获得的生物质，以及制备重组蛋白质的方法 通过生物量。 此外，本发明涉及一种回收稳定的重组细胞克隆的方法。
-
6.发明申请Recombinant Cell Clones Having Increased Stability and Methods of Making and Using the Same 有权增加稳定性的重组细胞克隆及其制备和使用方法公开(公告)号：US20100317055A1
公开(公告)日：2010-12-16
申请号：US12848897
申请日：2010-08-02
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
IPC分类号： C12P21/02
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
摘要翻译： 公开了稳定的重组细胞克隆，其在无血清和无蛋白质的培养基中稳定至少40代，通过在无血清和无蛋白质培养条件下倍增稳定细胞克隆获得的生物质，以及制备重组蛋白质的方法 通过生物量。 此外，本发明涉及一种回收稳定的重组细胞克隆的方法。
-
7.发明申请RECOMBINANT CELL CLONES HAVING INCREASED STABILITY AND METHODS OF MAKING AND USING THE SAME 有权具有增加的稳定性的重组细胞克隆及其制备和使用方法公开(公告)号：US20100221828A1
公开(公告)日：2010-09-02
申请号：US12778776
申请日：2010-05-12
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
IPC分类号： C12N5/10
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
摘要翻译： 公开了稳定的重组细胞克隆，其在无血清和无蛋白质的培养基中稳定至少40代，通过在无血清和无蛋白质培养条件下倍增稳定细胞克隆获得的生物质和制备重组蛋白的方法 通过生物量。 此外，本发明涉及一种回收稳定的重组细胞克隆的方法。
-
8.发明授权公开(公告)号：US06936441B2
公开(公告)日：2005-08-30
申请号：US10170661
申请日：2002-06-12
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
-
9.发明授权Recombinant cell clone having increased stability in serum- and protein-free medium and a method of recovering the stable cell clone and the production of recombinant proteins by using a stable cell clone 失效在无血清和无蛋白质培养基中具有增加的稳定性的重组细胞克隆和通过使用稳定的细胞克隆来恢复稳定的细胞克隆和重组蛋白的产生的方法
公开(公告)号：US6100061A
公开(公告)日：2000-08-08
申请号：US100253
申请日：1998-06-19
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0043 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clone which is stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
摘要翻译： 公开了一种稳定的重组细胞克隆，其在无血清和无蛋白质培养基中稳定至少40代，通过在无血清和无蛋白质培养条件下倍增稳定细胞克隆获得的生物质和制备重组蛋白的方法 通过生物量。 此外，本发明涉及一种回收稳定的重组细胞克隆的方法。
-
公开(公告)号：US08722406B2
公开(公告)日：2014-05-13
申请号：US13198476
申请日：2011-08-04
IPC分类号： C12N5/07
CPC分类号： C12P21/00 , C07K14/755 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0682 , C12N2500/05 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/60 , C12N2500/76 , C12N2500/92
摘要： A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.
摘要翻译： 描述了用于无蛋白质和无血清培养细胞，特别是哺乳动物细胞的培养基，由此培养基含有一定比例的大豆水解产物。
-
-
-
-
-
-
-
-
-
搜索结果
102 条记录 (耗时 0.025 秒)
