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    Polymers of isoprene from renewable resources
    4.
    发明申请
    Polymers of isoprene from renewable resources 有权

    公开(公告)号：US20110237769A1

    公开(公告)日：2011-09-29

    申请号：US12459399

    申请日：2009-06-30

    申请人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

    发明人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

    IPC分类号： C08F36/08 ,  C08F236/08 ,  C07C11/12 ,  G01N9/00

    CPC分类号： C08F236/18 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08F236/08 ,  C08F236/10 ,  C08F236/12 ,  C08L9/00 ,  C12P5/007 ,  Y10T436/24

    摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. In some cases, these cells are cultured in a culture medium that includes a carbon source, such as, but not limited to, a carbohydrate, glycerol, glycerine, dihydroxyacetone, one-carbon source, oil, animal fat, animal oil, fatty acid, lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, renewable carbon source, polypeptide (e.g., a microbial or plant protein or peptide), yeast extract, component from a yeast extract, or any combination of two or more of the foregoing. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. It is anticipated that there will be a significant demand for synthetic rubber and other isoprene containing polymers that are synthesized using isoprene of this type which is made from renewable, non-petrochemical based resources. In fact, it is believed that industrial customers and consumers would prefer to purchase isoprene containing polymers that are derived from such environmentally friendly sources to those that are made with isoprene derived from a petrochemical process. It is further believed that customers would be willing to pay premium prices for such environmentally friendly products that are made with renewable resources. However, it is important to be able to verify that such isoprene containing polymers are actually made from non-petrochemical based resources. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰. This type of polyisoprene can be a cis-1,4-polyisoprene homopolymer rubber.

    Methods of creating modified promoters resulting in varying levels of gene expression
    5.
    发明申请
    Methods of creating modified promoters resulting in varying levels of gene expression 审中-公开
    产生修饰的启动子的方法导致不同程度的基因表达

    公开(公告)号：US20060057633A1

    公开(公告)日：2006-03-16

    申请号：US10505844

    申请日：2003-04-18

    申请人： Marguerite Cervin ,  Fernando Valle

    发明人： Marguerite Cervin ,  Fernando Valle

    IPC分类号： C40B30/06 ,  C40B40/02

    CPC分类号： C12N15/1082 ,  C12N15/63 ,  C12N15/70 ,  C12N15/71 ,  C12N15/72 ,  C40B30/06 ,  C40B40/02 ,  C40B50/06

    摘要： The present invention relates to a method of creating promoter cassettes that include modified precursor promoters and transforming a population of bacterial host cells with a promoter library comprising the promoter cassettes resulting in bacterial clones having a range of expression levels of a gene of interest. The invention further relates to selecting a transformed bacterial host cell which has an optimum level of gene expression.

    摘要翻译： 本发明涉及一种产生启动子盒的方法，其包括经修饰的前体启动子并用包含启动子盒的启动子文库转化细菌宿主细胞群，从而产生具有一定目的基因表达水平范围的细菌克隆。 本发明还涉及选择具有最佳基因表达水平的转化细菌宿主细胞。