-
公开(公告)号：US20050079617A1
公开(公告)日：2005-04-14
申请号：US10728337
申请日：2003-12-03
摘要： A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
摘要翻译： 公开了一种用于将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS 细菌细胞的方法。 包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡萄糖激酶的多核苷酸。
-
2.发明申请Method of creating a library of bacterial clones with varying levels of gene expression 审中-公开创建具有不同基因表达水平的细菌克隆文库的方法公开(公告)号：US20060014146A1
公开(公告)日：2006-01-19
申请号：US10511043
申请日：2003-04-18
CPC分类号： C12N15/1082 , C12Q1/689
摘要： The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
摘要翻译： 本发明涉及一种创建包含人源启动子文库和/或修饰的核糖体结合位点文库的DNA文库的方法，并用文库转化细菌宿主细胞，以获得具有染色体表达水平范围的细菌克隆群 感兴趣的基因
-
3.发明申请公开(公告)号：US20060121581A1
公开(公告)日：2006-06-08
申请号：US10527827
申请日：2003-10-03
CPC分类号： C07K14/24 , C12N15/70 , C12P7/06 , C12P7/065 , C12P7/18 , C12P7/20 , C12P7/28 , C12P7/40 , C12P7/44 , C12P7/46 , C12P13/04 , C12P13/22 , Y02E50/17
摘要： The present invention provides methods to enhance production of desired products and increase the growth rate of a bacterial strain by inactivating an endogenous arcA and optionally overexpressing a ppc gene.
摘要翻译： 本发明提供了通过灭活内源性arcA和任选地过表达ppc基因来增强所需产物的产生和增加细菌菌株的生长速率的方法。
-
公开(公告)号：US08389214B2
公开(公告)日：2013-03-05
申请号：US11971341
申请日：2008-01-09
IPC分类号： C12Q1/68
摘要： A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
摘要翻译： 公开了一种将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS- / Glu-细菌细胞的方法。 包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡糖激酶的多核苷酸。
-
公开(公告)号：US20120288907A1
公开(公告)日：2012-11-15
申请号：US13471339
申请日：2012-05-14
摘要： A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
摘要翻译： 公开了一种将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS- / Glu-细菌细胞的方法。 包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡糖激酶的多核苷酸。
-
6.发明申请Method of Creating a Library of Bacterial Clones with Varying Levels of Gene Expression 有权创建不同基因表达水平的细菌克隆库的方法公开(公告)号：US20120015849A1
公开(公告)日：2012-01-19
申请号：US13155290
申请日：2011-06-07
CPC分类号： C12N15/1086 , C40B40/08 , C40B50/06
摘要： The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
摘要翻译： 本发明涉及一种创建包含人源启动子文库和/或修饰的核糖体结合位点文库的DNA文库的方法，并用文库转化细菌宿主细胞，以获得具有染色体表达水平范围的细菌克隆群 感兴趣的基因
-
公开(公告)号：US20090142843A1
公开(公告)日：2009-06-04
申请号：US11971341
申请日：2008-01-09
摘要： A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
摘要翻译： 公开了一种将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS- / Glu-细菌细胞的方法。 包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡糖激酶的多核苷酸。
-
公开(公告)号：US08476041B2
公开(公告)日：2013-07-02
申请号：US13471339
申请日：2012-05-14
IPC分类号： C12P21/02
摘要： A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
摘要翻译： 公开了一种将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS- / Glu-细菌细胞的方法。 包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡糖激酶的多核苷酸。
-
公开(公告)号：US08168608B2
公开(公告)日：2012-05-01
申请号：US12940225
申请日：2010-11-05
申请人： Mark S. Payne , Stephen K. Picataggio , Amy Kuang-Hsu , Ramesh Velayudhan Nair , Fernando Valle , Philippe Soucaille , Donald Eugene Trimbur
发明人： Mark S. Payne , Stephen K. Picataggio , Amy Kuang-Hsu , Ramesh Velayudhan Nair , Fernando Valle , Philippe Soucaille , Donald Eugene Trimbur
摘要： This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.
-
10.发明申请公开(公告)号：US20080176302A1
公开(公告)日：2008-07-24
申请号：US12053991
申请日：2008-03-24
IPC分类号： C12P7/18
摘要： The present invention provides a microorganism useful for biologically producing 1,3-propanediol from a fermentable carbon source at higher yield than was previously known. The complexity of the cofactor requirements necessitates the use of a whole cell catalyst for an industrial process that utilizes this reaction sequence to produce 1,3-propanediol. The invention provides a microorganism with disruptions in specified genes and alterations in the expression levels of specified genes that is useful in a higher yielding process to produce 1,3-propanediol.
摘要翻译： 本发明提供了一种可用于从可发酵碳源生产1,3-丙二醇的微生物，其产率高于之前已知的。 辅助因子要求的复杂性需要使用全细胞催化剂用于工业过程，其利用该反应顺序产生1,3-丙二醇。 本发明提供了一种在特定基因中具有破坏的微生物，并且在用于产生1,3-丙二醇的更高产率的方法中有用的特定基因的表达水平的改变。
-
-
-
-
-
-
-
-
-
搜索结果
75 条记录 (耗时 0.032 秒)
