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公开(公告)号：US07932064B2
公开(公告)日：2011-04-26
申请号：US10461925
申请日：2003-06-13
申请人： Mark J. Burk , Grace Desantis , Brian Morgan , Zoulin Zhu
发明人： Mark J. Burk , Grace Desantis , Brian Morgan , Zoulin Zhu
CPC分类号： C12P13/004 , C07C253/14 , C07C253/30 , C12P13/002 , C12P13/02 , C12P17/10 , C07C255/16
摘要： The invention provides novel processes for making ethyl-4-cyano-3-hydroxybutyrate, e.g., (R)-ethyl 4-cyano-3-hydroxybutyric acid, and 4-cyano-3-hydroxybutyric acid. The invention provides protocols for making and 4-cyano-3-hydroxybutyric acid and ethyl-4-cyano-3-hydroxybutyrate by whole cell processes, cell lysate processes, “one pot processes” and “multi-pot” processes using a variety of parameters.
摘要翻译： 本发明提供了制备乙基-4-氰基-3-羟基丁酸酯的新方法，例如（R）-4-氰基-3-羟基丁酸乙酯和4-氰基-3-羟基丁酸。 本发明提供了通过全细胞过程制备和4-氰基-3-羟基丁酸和4-氰基-3-羟基丁酸乙酯的方案，细胞裂解物方法，“一锅法”和“多锅”方法，使用各种 参数。
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![Processes for making [R]-ethyl 4-cyano-3 hydroxybutyric acid](/abs-image/US/2013/02/12/US08372608B2/abs.jpg.150x150.jpg)
公开(公告)号：US08372608B2
公开(公告)日：2013-02-12
申请号：US13053404
申请日：2011-03-22
申请人： Mark J. Burk , Grace DeSantis , Brian Morgan , Zuolin Zhu
发明人： Mark J. Burk , Grace DeSantis , Brian Morgan , Zuolin Zhu
CPC分类号： C12P13/004 , C07C253/14 , C07C253/30 , C12P13/002 , C12P13/02 , C12P17/10 , C07C255/16
摘要： The invention provides novel processes for making ethyl-4-cyano-3-hydroxybutyrate, e.g., (R)-ethyl 4-cyano-3-hydroxybutyric acid, and 4-cyano-3-hydroxybutyric acid. The invention provides protocols for making and 4-cyano-3-hydroxybutyric acid and ethyl-4-cyano-3-hydroxybutyrate by whole cell processes, cell lysate processes, “one pot processes” and “multi-pot” processes using a variety of parameters.
摘要翻译： 本发明提供了制备4-氰基-3-羟基丁酸乙酯的新方法，例如（R）-4-氰基-3-羟基丁酸乙酯和4-氰基-3-羟基丁酸。 本发明提供了通过全细胞过程，细胞裂解物过程，一锅法和多锅法使用各种参数制备4-氰基-3-羟基丁酸和4-氰基-3-羟基丁酸乙酯的方案。
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公开(公告)号：US20120010104A1
公开(公告)日：2012-01-12
申请号：US13165663
申请日：2011-06-21
申请人： David Weiner , Tim Hitchman , Lishan Zhao , Mark J. Burk , Grace Desantis , Sarah Richardson Hanson , Aileen Milan , Toby Richardson , Patti Kretz , William Greenberg
发明人： David Weiner , Tim Hitchman , Lishan Zhao , Mark J. Burk , Grace Desantis , Sarah Richardson Hanson , Aileen Milan , Toby Richardson , Patti Kretz , William Greenberg
IPC分类号： C40B30/06
CPC分类号： C12N15/1086 , C12P41/006
摘要： The invention provides growth selection methods for screening an environmental or a mixed population library of nucleic acids comprising uncharacterized nucleic acids for the presence of a nucleic acid encoding an enzyme of interest. In one aspect, methods for screening and identification of enzymes, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated from nucleic acids isolated from more than one organism for enzymes of interest, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases, are also provided.
摘要翻译： 本发明提供用于筛选包含未表征的核酸的核酸的环境或混合群体文库以用于存在编码目的酶的核酸的生长选择方法。 在一个方面，提供了用于筛选和鉴定酶的方法，例如转氨酶，腈水解酶，醛缩酶，环氧化物水解酶。 还提供了从多于一种生物体分离的核酸产生的基因文库的产生和筛选的方法，所述生物体用于所关心的酶，例如转氨酶，腈水解酶，醛缩酶，环氧化物水解酶。
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公开(公告)号：US20100009426A1
公开(公告)日：2010-01-14
申请号：US12556528
申请日：2009-09-09
申请人： Grace DeSantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Kelly Chatman , Robert Farwell
发明人： Grace DeSantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Kelly Chatman , Robert Farwell
IPC分类号： C12N9/78
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
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公开(公告)号：US20090176976A1
公开(公告)日：2009-07-09
申请号：US12397204
申请日：2009-03-03
申请人： Grace DESANTIS , Jennifer Ann Chaplin , Ellen Chi , Aileen Milan , Jay M. Short , David Weiner , Mark Madden , Mark J. Burk , Dan E. Robertson , Darcy Madden
发明人： Grace DESANTIS , Jennifer Ann Chaplin , Ellen Chi , Aileen Milan , Jay M. Short , David Weiner , Mark Madden , Mark J. Burk , Dan E. Robertson , Darcy Madden
IPC分类号： C12N15/11
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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6.发明申请Nitrilases, Nucleic Acids Encoding Them and Methods For Making and Using Them 失效Nitrilases，Nucleic Acids编码他们和制造和使用它们的方法公开(公告)号：US20120009637A1
公开(公告)日：2012-01-12
申请号：US13236862
申请日：2011-09-20
申请人： Grace Desantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Robert Farwell , Kelly Chatman
发明人： Grace Desantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Robert Farwell , Kelly Chatman
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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7.发明申请Nitrilases, Nucleic Acids Encoding Them and Methods for Making and Using Them 有权Nitrilases，编码它们的核酸及其制备和使用方法公开(公告)号：US20100216214A1
公开(公告)日：2010-08-26
申请号：US12773317
申请日：2010-05-04
申请人： Grace Desantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Robert Farwell , Kelly Chatman
发明人： Grace Desantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Robert Farwell , Kelly Chatman
IPC分类号： C12N9/78
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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8.发明授权Methods for the manufacture of pure single enantiomer compounds and for selecting enantioselective enzymes 有权制备纯单一对映体化合物和选择对映选择性酶的方法公开(公告)号：US07981844B2
公开(公告)日：2011-07-19
申请号：US10178938
申请日：2002-06-21
申请人： David Weiner , Tim Hitchman , Lishan Zhao , Mark J. Burk , Grace Desantis , Sarah Richardson Hanson , Aileen Milan , Toby Richardson , Patti Kretz , William Greenberg
发明人： David Weiner , Tim Hitchman , Lishan Zhao , Mark J. Burk , Grace Desantis , Sarah Richardson Hanson , Aileen Milan , Toby Richardson , Patti Kretz , William Greenberg
CPC分类号： C12N15/1086 , C12P41/006
摘要： The invention provides biocatalytic methods for the manufacture of pure single enantiomer compounds. This invention provides methods of screening for enzymes which are highly enantioselective or enzymes that can provide any desired stereoisomer of a compound. The invention provides the use of single enantiomer substrates in performing a growth screen of a clonal library to identify highly stereoselective enzymes. In one aspect, methods for screening and identification of enzymes, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated from nucleic acids isolated from more than one organism for enzyme, e.g., transaminase, activities are also provided.
摘要翻译： 本发明提供用于制造纯单一对映异构体化合物的生物催化方法。 本发明提供了筛选高度对映选择性的酶的方法或可以提供化合物的任何所需立体异构体的酶的方法。 本发明提供单一对映异构体底物在克隆文库的生长筛选中鉴定高度立体选择性酶的用途。 在一个方面，提供了用于筛选和鉴定酶的方法，例如转氨酶，腈水解酶，醛缩酶，环氧化物水解酶。 还提供了用于生产和筛选由多于一种生物体分离的酶（例如转氨酶）活性产生的基因文库产生的方法。
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公开(公告)号：US08318471B2
公开(公告)日：2012-11-27
申请号：US13236862
申请日：2011-09-20
申请人： Grace Desantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Robert Farwell , Kelly Chatman
发明人： Grace Desantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Robert Farwell , Kelly Chatman
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
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公开(公告)号：US07651849B2
公开(公告)日：2010-01-26
申请号：US12397204
申请日：2009-03-03
申请人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay M. Short , David Paul Weiner , Mark Madden , Darcy Madden, legal representative , Mark J. Burk , Dan E. Robertson
发明人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay M. Short , David Paul Weiner , Mark Madden , Mark J. Burk , Dan E. Robertson
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , A61K38/00 , C07H21/04 , C12Q1/68 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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