Methods of separating particles using an optical gradient
    1.
    发明授权
    Methods of separating particles using an optical gradient 有权
    使用光学梯度分离颗粒的方法

    公开(公告)号:US06744038B2

    公开(公告)日:2004-06-01

    申请号:US09993326

    申请日:2001-11-14

    IPC分类号: H05H302

    摘要: Apparatus and methods are provided for interacting light with particles, including but not limited to biological matter such as cells, in unique and highly useful ways. Optophoresis consists of subjecting particles to various optical forces, especially optical gradient forces, and more particularly moving optical gradient forces, so as to obtain useful results. In biology, this technology represents a practical approach to probing the inner workings of a living cell, preferably without any dyes, labels or other markers. The invention includes methods for separating particles in a medium where the particles having differing dielectric constants by providing a medium having a dielectric constant between the dielectric constants of the particles, subjecting the particles in the media to an optical gradient field, and separating the particles.

    摘要翻译: 提供了用于以独特且非常有用的方式将光与颗粒(包括但不限于生物物质如细胞)相互作用的装置和方法。 电渗疗法包括使颗粒经受各种光学力,特别是光学梯度力,尤其是移动的光学梯度力,从而获得有用的结果。 在生物学中,该技术代表了探测活细胞内部运作的实际方法,优选没有任何染料,标签或其他标记物。 本发明包括通过提供具有介电常数介于介质常数之间的介质,使介质中的颗粒经受光学梯度场并分离颗粒的介质,分离具有不同介电常数的介质的介质中的颗粒的方法。

    METHODS AND APPARATUS FOR USE OF OPTICAL FORCES FOR IDENTIFICATION, CHARACTERIZATION AND/OR SORTING OF PARTICLES
    2.
    发明申请
    METHODS AND APPARATUS FOR USE OF OPTICAL FORCES FOR IDENTIFICATION, CHARACTERIZATION AND/OR SORTING OF PARTICLES 审中-公开
    用于识别,表征和/或分配颗粒的光学强度的方法和装置

    公开(公告)号:US20100108577A1

    公开(公告)日:2010-05-06

    申请号:US12545624

    申请日:2009-08-21

    IPC分类号: B07C5/36

    摘要: Apparatus and methods are provided for interacting light with particles, including but not limited to biological matter such as cells, in unique and highly useful ways. Optophoresis consists of subjecting particles to various optical forces, especially optical gradient forces, and more particularly moving optical gradient forces, so as to obtain useful results. In biology, this technology represents a practical approach to probing the inner workings of a living cell, preferably without any dyes, labels or other markers. In one aspect, a particle may be characterized by determining its optophoretic constant or signature. For example, a diseased cell has a different optophoretic constant from a healthy cell, thereby providing information, or the basis for sorting. In the event of physical sorting, various forces may be used for separation, including fluidic forces, such as through the use of laminar flow, or optical forces, or mechanical forces, such as through adhesion. Various techniques for measuring the dielectric constant of particles are provided.

    摘要翻译: 提供了用于以独特且非常有用的方式将光与颗粒(包括但不限于生物物质如细胞)相互作用的装置和方法。 电渗疗法包括使颗粒经受各种光学力,特别是光学梯度力,尤其是移动的光学梯度力,从而获得有用的结果。 在生物学中,该技术代表了探测活细胞内部运作的实际方法,优选没有任何染料,标签或其他标记物。 在一个方面,粒子的特征可以在于确定其荧光特性常数或特征。 例如,患病细胞与健康细胞具有不同的视觉波动常数,从而提供信息或分选的基础。 在物理分选的情况下,各种力可以用于分离,包括流体力,例如通过使用层流,或光学力,或机械力,例如通过粘附。 提供了用于测量颗粒的介电常数的各种技术。

    Method for separation of particles
    3.
    发明授权
    Method for separation of particles 有权
    分离颗粒的方法

    公开(公告)号:US06815664B2

    公开(公告)日:2004-11-09

    申请号:US09993318

    申请日:2001-11-14

    IPC分类号: H01S300

    摘要: Apparatus and methods are provided for interacting light with particles, including but not limited to biological matter such as cells, in unique and highly useful ways. Optophoresis consists of subjecting particles to various optical forces, especially optical gradient forces, and more particularly moving optical gradient forces, so as to obtain useful results. In biology, this technology represents a practical approach to probing the inner workings of a living cell, preferably without any dyes, labels or other markers. In one aspect, a method is provided for separating particles by flowing the particles within a first constrained path, the first constrained path having an input and an output, and a sorting region, the sorting region coupling to a second constrained path, the second constrained path including an output, illuminating the sorting region with a moving optical gradient, characterized in that certain of the particles flow in a laminar manner between the first inlet and the output of the first constrained path, and selected particles are diverted from the first constrained path to the second constrained path under the force of the moving optical gradient.

    摘要翻译: 提供了用于以独特且非常有用的方式将光与颗粒(包括但不限于生物物质如细胞)相互作用的装置和方法。 电渗疗法包括使颗粒经受各种光学力,特别是光学梯度力,尤其是移动的光学梯度力,从而获得有用的结果。 在生物学中,该技术代表了探测活细胞内部运作的实际方法,优选没有任何染料,标签或其他标记物。 在一个方面,提供了一种用于通过在第一约束路径内流动所述粒子来分离颗粒的方法,所述第一约束路径具有输入和输出,以及分选区域,所述分选区域耦合到第二约束路径,所述第二约束路径 路径,其包括输出,用移动的光学梯度照亮分选区域,其特征在于,某些颗粒以层状方式在第一约束路径的第一入口和输出之间流动,并且选定的颗粒从第一约束路径 到移动光学梯度的力的第二约束路径。

    Method of separating particles using an optical gradient
    5.
    发明授权
    Method of separating particles using an optical gradient 有权
    使用光学梯度分离颗粒的方法

    公开(公告)号:US06784420B2

    公开(公告)日:2004-08-31

    申请号:US09993377

    申请日:2001-11-14

    IPC分类号: H01S300

    摘要: Apparatus and methods are provided for interacting light with particles, including but not limited to biological matter such as cells, in unique and highly useful ways. Optophoresis consists of subjecting particles to various optical forces, especially optical gradient forces, and more particularly moving optical gradient forces, so as to obtain useful results. In biology, this technology represents a practical approach to probing the inner workings of a living cell, preferably without any dyes, labels or other markers. In one aspect, a method is provided for interacting an optical gradient field in three dimensions with a particle by interfering two beams to generate a plurality of planar fronts, providing a plurality of particles in a medium, and moving the planar fronts relative to the particles, whereby the particles are separated at least in part based upon the dielectric constant of the particles.

    摘要翻译: 提供了用于以独特且非常有用的方式将光与颗粒(包括但不限于生物物质如细胞)相互作用的装置和方法。 电渗疗法包括使颗粒经受各种光学力,特别是光学梯度力,尤其是移动的光学梯度力,从而获得有用的结果。 在生物学中,该技术代表了探测活细胞内部运作的实际方法,优选没有任何染料,标签或其他标记物。 在一个方面,提供了一种用于通过干扰两个光束来将三维光学梯度场与粒子相互作用以产生多个平面前沿,在介质中提供多个粒子并相对于粒子移动平面前沿的方法 其中颗粒至少部分地基于颗粒的介电常数被分离。