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公开(公告)号：US4680261A
公开(公告)日：1987-07-14
申请号：US645761
申请日：1984-08-30
CPC分类号： C07K14/555 , Y10S435/811
摘要： A process for producing interferon comprises culturing interferon-producing mammalian cells in a cell culture medium containing at least one compound selected from the group consisting of ascorbic acid, an ascorbic acid derivative and a vanadium compound. According to the process of the present invention, interferon can be produced in large amounts.
摘要翻译： 制备干扰素的方法包括在含有至少一种选自抗坏血酸，抗坏血酸衍生物和钒化合物的化合物的细胞培养基中培养产生干扰素的哺乳动物细胞。 根据本发明的方法，可以大量生产干扰素。
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公开(公告)号：US4548900A
公开(公告)日：1985-10-22
申请号：US496506
申请日：1983-05-20
申请人： Masahiro Nobuhara , Kiyoshi Yamaguchi , Ei Mochida
发明人： Masahiro Nobuhara , Kiyoshi Yamaguchi , Ei Mochida
CPC分类号： C07K14/56 , C07K14/565 , Y10S435/948
摘要： A method for the mass production of interferon, wherein cultured cells are brought into contact with at least one polyhydric alcohol, thereby achieving a remarkable increase in the production of interferon from the cultured cells.
摘要翻译： 一种大规模生产干扰素的方法，其中使培养的细胞与至少一种多元醇接触，从而使培养细胞的干扰素产生显着增加。
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公开(公告)号：US4465622A
公开(公告)日：1984-08-14
申请号：US385872
申请日：1982-06-07
申请人： Masahiro Nobuhara , Kiyoshi Yamaguchi , Ei Mochida
发明人： Masahiro Nobuhara , Kiyoshi Yamaguchi , Ei Mochida
IPC分类号： C12P21/00 , A61K38/21 , C07K1/16 , C07K1/22 , C07K14/52 , C07K14/555 , C07K14/56 , C07K14/565 , C07C103/52 , A61K45/02
CPC分类号： C07K14/565 , C07K14/56
摘要： A method for purifying interferon having anti-cancer effects or anti-viral effects, wherein highly purified interferon can be easily obtained by a single operation in a recovery of about 100%. In this method, interferon is obtained by specifically adsorbing interferon onto a carrier containing acrylonitrile polymer and eluting the adsorbed interferon with an appropriate buffer.
摘要翻译： 一种用于纯化具有抗癌作用或抗病毒效果的干扰素的方法，其中高纯度的干扰素可以通过单次操作容易地获得，回收率为约100％。 在该方法中，通过将干扰素特异性吸附到含有丙烯腈聚合物的载体上并用合适的缓冲液洗脱吸附的干扰素来获得干扰素。
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公开(公告)号：US5459045A
公开(公告)日：1995-10-17
申请号：US149917
申请日：1993-11-10
申请人： Hiroshi Sato , Tadakazu Yamauchi , Toshio Izako , Masahiro Nobuhara , Ei Mochida
发明人： Hiroshi Sato , Tadakazu Yamauchi , Toshio Izako , Masahiro Nobuhara , Ei Mochida
IPC分类号： G01N33/53 , G01N33/531 , G01N33/532 , G01N33/557 , G01N33/577 , G01N33/58 , G01N33/74 , G01N33/552
CPC分类号： G01N33/743 , G01N33/532 , G01N33/557 , G01N33/58 , Y10S436/817
摘要： A reagent for use in an immunoassay for measuring haptens, antigens or antibodies by means of a competitive binding method, which comprises a combination of an antibody and a labelled hapten or a labelled antigen or a combination of a hapten or an antigen and a labelled antibody, wherein the antibody and the labelled hapten or the labelled antigen in one combination or the hapten or the antigen and the labelled antibody in another combination are capable of undergoing reversible binding, and a device for use in an immunoassay wherein the reagent of the present invention is included in a single container.An immunoassay can be performed in a short time by the use of the immunoassay device of the present invention.
摘要翻译： 用于通过竞争性结合方法测量半抗原，抗原或抗体的免疫测定的试剂，其包含抗体和标记的半抗原或标记抗原或半抗原或抗原和标记抗体的组合的组合 其中一个组合中的抗体和标记的半抗原或标记抗原或另一组合中的半抗原或抗原和标记的抗体能够进行可逆结合，以及用于免疫测定的装置，其中本发明的试剂 包含在一个容器中。 可以通过使用本发明的免疫测定装置在短时间内进行免疫测定。
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公开(公告)号：US5283176A
公开(公告)日：1994-02-01
申请号：US480165
申请日：1990-02-14
申请人： Hiroshi Sato , Tadakazu Yamauchi , Toshio Izako , Masahiro Nobuhara , Ei Mochida
发明人： Hiroshi Sato , Tadakazu Yamauchi , Toshio Izako , Masahiro Nobuhara , Ei Mochida
IPC分类号： G01N33/53 , G01N33/531 , G01N33/532 , G01N33/557 , G01N33/577 , G01N33/58 , G01N33/74 , C12Q1/00
CPC分类号： G01N33/743 , G01N33/532 , G01N33/557 , G01N33/58 , Y10S436/817
摘要： A reagent for use in an immunoassay for measuring haptens, antigens or antibodies by means of a competitive binding method, which comprises a combination of an antibody and a labelled hapten or a labelled antigen or a combination of a hapten or an antigen and a labelled antibody, wherein the antibody and the labelled hapten or the labelled antigen in one combination or the hapten or the antigen and the labelled antibody in another combination are capable of undergoing reversible binding, and a device for use in an immunoassay wherein the reagent of the present invention is included in a single container. This immunoassay can be performed in a short time by the use of the immunoassay device of the present invention.
摘要翻译： 用于通过竞争性结合方法测量半抗原，抗原或抗体的免疫测定的试剂，其包含抗体和标记的半抗原或标记抗原或半抗原或抗原和标记抗体的组合的组合 其中一个组合中的抗体和标记的半抗原或标记抗原或另一组合中的半抗原或抗原和标记的抗体能够进行可逆结合，以及用于免疫测定的装置，其中本发明的试剂 包含在一个容器中。 可以通过使用本发明的免疫测定装置在短时间内进行该免疫测定。
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6.发明授权Polypeptide, DNA fragment encoding the same, drug composition containing the same and process for producing the same 失效多肽，编码相同的DNA片段，含有其的药物组合物及其制备方法
公开(公告)号：US5451659A
公开(公告)日：1995-09-19
申请号：US972387
申请日：1992-11-05
CPC分类号： C07K14/811 , A61K38/00
摘要： This invention particularly provides a novel polypeptide having high protease-inhibiting activity, preferably FXa-inhibiting activity, which comprises, at least as a part of the polypeptide, an amino acid sequence resulting from substitution of an amino acid for at least one amino acid in the following amino acid sequence (1), wherein the amino acid substitution is at least one substitution selected from the following substitution means (i) to (iii). It also provides a process for the production of the polypeptide, a novel DNA fragment encoding the polypeptide and a drug composition containing the same.______________________________________ Amino acid sequence (1) ______________________________________ Cys Asn Leu Pro Ile Val Arg Gly Pro Cys Arg Ala Phe Ile Gln Leu Trp Ala Phe Asp Ala Val Lys Gly Lys Cys Val Leu Phe Pro Tyr Gly Gly Cys Gln Gly Asn Gly Asn Lys Phe Tyr Ser Glu Lys Glu Cys Arg Glu Tyr Cys ______________________________________ (i) Substitution of 15 position Gln counting from the N-terminus by an amino acid other than Gln.(ii) Substitution of 42 position Tyr counting from the N-terminus by an amino acid other than Tyr.(iii) Substitution of 7 position Arg counting from the N-terminus by an amino acid other than Arg.
摘要翻译： 本发明特别提供了一种具有高蛋白酶抑制活性，优选为FXa抑制活性的新型多肽，其至少作为多肽的一部分，由氨基酸取代至少一个氨基酸而产生的氨基酸序列 以下氨基酸序列（1），其中所述氨基酸取代为选自以下取代方案（i）至（iii）中的至少一种取代基。 它还提供了制备多肽的方法，编码多肽的新型DNA片段和含有该多肽的药物组合物。 - 氨基酸序列（1） - Cys Asn Leu Pro Ile Val Arg Gly Pro Cys - Arg Ala Phe Ile Gln Leu Trp Ala Phe Asp - Ala Val Lys Gly Lys Cys Val Leu Phe Pro - Tyr Gly Gly Cys Gln Gly Asn Gly Asn Lys-Phe Tyr Ser Glu Lys Glu Cys Arg Glu Tyr - Cys - （i）通过除Gln之外的氨基酸从N末端取代15位Gln。 （ii）用除Tyr之外的氨基酸从N末端取代42位Tyr计数。 （iii）用除Arg以外的氨基酸从N末端取代7位Arg。
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7.发明授权Process for specific binding assay for measuring the amount of analyte in a liquid test sample 失效用于测量液体测试样品中分析物的量的特异性结合测定法公开(公告)号：US06218134B1
公开(公告)日：2001-04-17
申请号：US08637905
申请日：1996-04-26
IPC分类号： G01N3353
CPC分类号： G01N33/5438 , G01N33/54386 , G01N33/558 , Y10S435/81 , Y10S435/962 , Y10S435/97 , Y10S436/807
摘要： A specific binding assay process which is useful for quick qualitative or quantitative measurement and which can be used for various purposes, and a specific binding assay device suitable for the practice of the process, in which a substance to be assayed in a liquid test sample is determined qualitatively or quantitatively by developing the substance in a liquid test sample in a matrix, which comprises the steps of, for example: allowing the substance to be assayed to reach with a specific binding substance which has specific affinity for the substance to be assayed; allowing a signal substance generator (a substance which competes with the substance to be assayed for the specific binding substance and which generates a signal) to change its distribution in the matrix in response to the reaction of the above step, and; detecting the resulting distributional changes at a detection means as changes of signals which are rate-limited by the mass transfer of a signal substance generated from the signal substance generator.
摘要翻译： 可用于快速定性或定量测量并可用于各种目的的特异性结合测定方法，以及适于实施该方法的特异性结合测定装置，其中待测物质在液体测试样品中为 通过在基质中液体测试样品中开发物质定性或定量地测定，其包括以下步骤：允许用特异性结合物质测定物质以对待测物质具有特异性亲和力; 允许响应于上述步骤的反应使信号物质发生器（与物质竞争的物质与特定结合物质进行测定并产生信号）改变其在基质中的分布，并且检测所得到的分布 在由t产生的信号物质的传质速率限制的信号变化时，在检测装置处的变化 他信号物质发生器。
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公开(公告)号：US5516644A
公开(公告)日：1996-05-14
申请号：US243095
申请日：1994-05-16
IPC分类号： G01N33/542 , G01N33/543 , G01N33/557 , G01N33/558 , G01N33/535 , G01N33/544 , G01N33/551 , G01N33/561
CPC分类号： G01N33/5438 , G01N33/54386 , G01N33/558 , Y10S435/81 , Y10S435/962 , Y10S435/97 , Y10S436/807
摘要： A specific binding assay process which is useful for quick qualitative or quantitative measurement and which can be used for various purposes, and a specific binding assay device suitable for the practice of the process,in which a substance to be assayed in a liquid test sample is determined qualitatively or quantitatively by developing the substance in a liquid test sample in a matrix, which comprises the steps of, for example:allowing the substance to be assayed to react with a specific binding substance which has specific affinity for the substance to be assayed;allowing a signal substance generator (a substance which competes with the substance to be assayed for the specific binding substance and which generates a signal) to chance its distribution in the matrix in response to the reaction of the above step, and;detecting the resulting distributional changes at a detection means as changes of signals which are rate-limited by the mass transfer of a signal substance generated from the signal substance generator.
摘要翻译： 可用于快速定性或定量测量并可用于各种目的的特异性结合测定方法，以及适于实施该方法的特异性结合测定装置，其中待测物质在液体测试样品中为 通过在基质中液体测试样品中开发物质进行定性或定量测定，其包括以下步骤：使待测物质与对待测物质具有特异性亲和力的特异性结合物质反应; 允许响应于上述步骤的反应使信号物质发生器（与物质竞争的物质被测定为特异性结合物质并产生信号）偶然地分布在基质中; 在检测装置处检测所得到的分布变化，作为通过由信号物质发生器产生的信号物质的质量传递而受速率限制的信号的变化。
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公开(公告)号：US5792629A
公开(公告)日：1998-08-11
申请号：US293150
申请日：1994-08-19
IPC分类号： A61K38/55 , A61K38/00 , A61P1/00 , A61P1/16 , A61P7/02 , A61P9/08 , A61P9/10 , A61P11/16 , A61P13/02 , A61P15/00 , A61P29/00 , A61P37/08 , C07K14/81 , C12N1/21 , C12N9/99 , C12N15/09 , C12N15/15 , C12P21/02 , C12Q1/37 , C12R1/19 , C12N5/10 , C12N15/63
CPC分类号： C07K14/8114 , A61K38/005 , C12Q1/37
摘要： This invention provides a novel polypeptide which comprises an amino acid sequence that constitutes a portion of urinary trypsin inhibitor (UTI) and which has no antigenicity against human and high activity to inhibit various proteases, as well as other novel polypeptides having excellent activities to inhibit various proteases obtained by mutation of the former novel polypeptide. This invention also provides novel enzyme inhibition processes, drug compositions and treating methods making use of the novel polypeptide, DNA fragments containing nucleotide sequences which encode the novel polypeptides, vectors containing the DNA fragments and transformants transformed with the DNA fragments or the vectors, as well as processes for the production of the novel polypeptides.
摘要翻译： 本发明提供了一种新型多肽，其包含构成尿胰蛋白酶抑制剂（UTI）的一部分的氨基酸序列，并且其对人体没有抗原性和抑制各种蛋白酶的高活性，以及具有优异的抑制各种蛋白酶活性的新型多肽 通过前一种新型多肽突变获得的蛋白酶。 本发明还提供新的酶抑制过程，药物组合物和利用新多肽的治疗方法，含有编码新多肽的核苷酸序列的DNA片段，含有DNA片段的载体和用DNA片段或载体转化的转化体 作为生产新型多肽的方法。
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10.发明授权Polypeptide, DNA fragment encoding the same, drug composition containing the same and process for producing the same 失效多肽，编码相同的DNA片段，含有其的药物组合物及其制备方法
公开(公告)号：US5589360A
公开(公告)日：1996-12-31
申请号：US431412
申请日：1995-04-28
CPC分类号： C07K14/811 , A61K38/00
摘要： This invention particularly provides a novel polypeptide having high protease-inhibiting activity, preferably FXa-inhibiting activity, which comprises, an lease as a part of the polypeptide, an amino acid sequence resulting from substitution of an amino acid for at least one amino acid in the following amino acid sequence (1), wherein the amino acid substitution is at least one substitution selected from the following substitution means (i) to (iii). It also provides a process for the production of the polypeptide, a novel DNA fragment encoding the polypeptide and a drug composition containing the same.______________________________________ Amino acid sequence (1) ______________________________________ Cys Asn Leu Pro Ile Val Arg Gly Pro Cys Arg Ala Phe Ile Gln Leu Trp Ala Phe Asp Ala Val Lys Gly Lys Cys Val Leu Phe Pro Tyr Gly Gly Cys Gln Gly Asn Gly Asn Lys Phe Tyr Ser Glu Lys Glu Cys Arg Glu Tyr Cys ______________________________________ (i) Substitution of 15 position Gln counting from the N-terminus by an amino acid other than Gln.(ii) Substitution of 42 position Tyr counting from the N-terminus by an amino acid other than Tyr.(iii) Substitution of 7 position Arg counting from the N-terminus by an amino acid other than Arg.
摘要翻译： 本发明特别提供了具有高蛋白酶抑制活性，优选FXa抑制活性的新型多肽，其包括作为多肽的一部分的租赁，由氨基酸取代至少一个氨基酸得到的氨基酸序列 以下氨基酸序列（1），其中所述氨基酸取代为选自以下取代方案（i）至（iii）中的至少一种取代基。 它还提供了制备多肽的方法，编码多肽的新型DNA片段和含有该多肽的药物组合物。 - 氨基酸序列（1） - Cys Asn Leu Pro Ile Val Arg Gly Pro Cys - Arg Ala Phe Ile Gln Leu Trp Ala Phe Asp - Ala Val Lys Gly Lys Cys Val Leu Phe Pro - Tyr Gly Gly Cys Gln Gly Asn Gly Asn Lys-Phe Tyr Ser Glu Lys Glu Cys Arg Glu Tyr - Cys - （i）通过除Gln之外的氨基酸从N末端取代15位Gln。 （ii）用除Tyr之外的氨基酸从N末端取代42位Tyr计数。 （iii）用除Arg以外的氨基酸从N末端取代7位Arg。
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