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1.发明授权Anti-glycated hemoglobin monoclonal antibody and method for measuring glycated hemoglobin 失效抗糖化血红蛋白单克隆抗体及测定糖化血红蛋白的方法
公开(公告)号：US5470759A
公开(公告)日：1995-11-28
申请号：US73340
申请日：1993-06-07
IPC分类号： G01N33/50 , C07K16/18 , C12P21/08 , G01N33/72 , G01N33/538
CPC分类号： C07K16/18 , G01N33/723
摘要： A method for measuring glycated hemoglobin is disclosed, which comprises contacting a sample with an anti-hemoglobin antibody being bound to a solid phase, adding thereto an anti-glycated hemoglobin antibody, and then measuring the anti-glycated hemoglobin antibody bound to the solid phase via glycated hemoglobin and the anti-hemoglobin antibody. A method for measuring glycated hemoglobin is further disclosed, which comprises mixing a sample with particles on which an anti-glycated hemoglobin antibody is bound to cause immunoagglutination reaction. A method for measuring glycated hemoglobin is furthermore disclosed, which comprises mixing a sample with a solution containing a monoclonal antibody specific for glycated hemoglobin, and then measuring the change in the turbidity of the resulting mixture. A method is disclosed for measuring glycated hemoglobin which comprises mixing a sample with unsensitized latex particles and an anti-glycated hemoglobin antibody, to cause immunoagglutination reaction. There is disclosed a method for measuring glycated hemoglobin which comprises mixing a sample with unsensitized latex particles and particles on which an anti-glycated hemoglobin antibody is bound, to cause immunoagglutination reaction. Still furthermore, an anti-glycated hemoglobin monoclonal antibody having a high specificity for human glycated hemoglobin is disclosed.
摘要翻译： 公开了一种测定糖化血红蛋白的方法，其包括将样品与结合于固相的抗血红蛋白抗体接触，向其中加入抗糖化血红蛋白抗体，然后测定与固相结合的抗糖化血红蛋白抗体 通过糖化血红蛋白和抗血红蛋白抗体。 进一步公开了测定糖化血红蛋白的方法，其包括将样品与其上结合有抗糖基化血红蛋白抗体的颗粒混合以引起免疫凝集反应。 还公开了一种测定糖化血红蛋白的方法，其包括将样品与含有糖化血红蛋白特异性的单克隆抗体的溶液混合，然后测量所得混合物的浊度变化。 公开了一种用于测量糖化血红蛋白的方法，其包括将样品与未敏化的胶乳颗粒和抗糖化血红蛋白抗体混合以引起免疫凝集反应。 公开了一种测定糖化血红蛋白的方法，其包括将样品与未敏化的胶乳颗粒和抗糖基化血红蛋白抗体结合的颗粒混合，引起免疫凝集反应。 此外，公开了对人糖化血红蛋白具有高特异性的抗糖化血红蛋白单克隆抗体。
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公开(公告)号：US5093081A
公开(公告)日：1992-03-03
申请号：US369332
申请日：1989-06-21
申请人： Yukio Sudo , Yoshihiro Ashihara , Toshikage Hiraoka , Isao Nishizono , Shigeki Kageyama , Tetsuji Tanimoto
发明人： Yukio Sudo , Yoshihiro Ashihara , Toshikage Hiraoka , Isao Nishizono , Shigeki Kageyama , Tetsuji Tanimoto
IPC分类号： G01N33/543
CPC分类号： G01N33/54386
摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-insoluble macromolecular substrate, and(B) an antibody, reacting with the ligand in the sample, conjugated with an enzyme capable of acting on the above water-insoluble macromolecular substrate.The above analytical substance further comprises,(C) a macromolecular substance which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct a highly sensitive and simple enzyme immunoassay.
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公开(公告)号：US07276343B2
公开(公告)日：2007-10-02
申请号：US10518586
申请日：2003-06-24
IPC分类号： G01N33/53 , G01N33/542 , G01N33/543 , G01N33/553 , G01N33/566 , G01N21/75 , G01N21/76 , C12Q1/00 , C12N9/00 , C09K3/00
CPC分类号： G01N33/533 , G01N21/76 , G01N33/532
摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.
摘要翻译： 本发明提供一种化学发光增强剂，其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂，其用于固相免疫测定中的信号检测，所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上，该水溶液由水溶性大分子季铵盐，季锍盐或季鏻 盐，以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射，其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理，以及化学发光 方法和使用化学发光增强剂的试剂盒。
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公开(公告)号：US06589754B1
公开(公告)日：2003-07-08
申请号：US08946685
申请日：1997-10-08
申请人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
发明人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
IPC分类号： G01N3353
CPC分类号： G01N33/54386
摘要： An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.
摘要翻译： 一种用于通过测定由高分子聚合物抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析大分子抗原的免疫测定元件。 免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 还提供了通过使用免疫测定元件定量分析样品中的大分子抗原的方法。
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公开(公告)号：US5603898A
公开(公告)日：1997-02-18
申请号：US993964
申请日：1992-12-17
申请人： Yoshihiro Ashihara , Yukio Sudo , Isao Nishizono , Toshikage Hiraoka , Tetsuji Tanimoto , Shigeki Kageyama
发明人： Yoshihiro Ashihara , Yukio Sudo , Isao Nishizono , Toshikage Hiraoka , Tetsuji Tanimoto , Shigeki Kageyama
CPC分类号： G01N33/581 , G01N33/54386
摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.
摘要翻译： 一种用于免疫测定的干式分析元件，其具有至少一个用于根据酶免疫测定法测量样品中的配体的透水层，其包含（A）水溶性大分子底物和（B）与抗体结合的酶 与能够作用于上述水溶性大分子底物的样品中的配体反应。 上述分析元素还包含（C）在上述干式分析元件的上述透水层中作为配体或其衍生物与高分子化合物的共轭物的高分子抗原。 本发明可以进行高灵敏度和简单的酶免疫测定。
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公开(公告)号：US5569589A
公开(公告)日：1996-10-29
申请号：US163405
申请日：1993-12-07
申请人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Naofumi Hora , Yoshihiro Ashihara , Yukio Sudo , Toshihiro Mori
发明人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Naofumi Hora , Yoshihiro Ashihara , Yukio Sudo , Toshihiro Mori
IPC分类号： G01N31/22 , G01N33/542 , G01N33/543 , G01N33/53
CPC分类号： G01N33/54386 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.
摘要翻译： 一种免疫测定元件，用于通过测定配体，配体与高分子化合物的连接产物和酶标记的抗体之间的反应引起的酶活性的变化来定量分析配体。 免疫测定元件包括含有在酶存在下形成可扩散材料的非扩散性底物的底物层和含有用于进一步碎裂非可弥散材料的碎裂酶的试剂层。 还提供了通过使用免疫测定元件定量分析样品中所含的配体的方法。
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公开(公告)号：US20070059786A1
公开(公告)日：2007-03-15
申请号：US11447839
申请日：2006-06-07
IPC分类号： G01N33/551 , C07D305/02
CPC分类号： G01N33/533 , G01N21/76 , G01N33/532
摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.
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公开(公告)号：US20050239149A1
公开(公告)日：2005-10-27
申请号：US10518586
申请日：2003-06-24
IPC分类号： G01N21/76 , G01N33/532 , G01N33/533 , G01N33/53 , G01N33/537 , G01N33/543 , C07D35/02
CPC分类号： G01N33/533 , G01N21/76 , G01N33/532
摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.
摘要翻译： 本发明提供一种化学发光增强剂，其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂，其用于固相免疫测定中的信号检测，所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上，该水溶液由水溶性大分子季铵盐，季锍盐或季鏻 盐，以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射，其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理和化学发光 方法和使用化学发光增强剂的试剂盒。
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9.发明授权Treponema pallidum fused antigen and assay for anti-treponema pallidum antibodies using the same fused antigen 有权梅毒螺旋体融合抗原，并使用相同的融合抗原测定抗梅毒螺旋体pallidum抗体公开(公告)号：US06794155B2
公开(公告)日：2004-09-21
申请号：US09761667
申请日：2001-01-18
IPC分类号： G01N3353
CPC分类号： C07K14/20 , C07K2319/00 , Y10S530/806 , Y10S530/82
摘要： A Treponema pallidum fused antigen in which at least two surface antigens of Treponema pallidum are fused and an assay for anti-Treponema pallidum antibodies, using the above Treponema pallidum fused antigen.
摘要翻译： 使用上述梅毒螺旋体融合抗原，梅毒螺旋体融合抗原（其中将梅毒螺旋体的至少两个表面抗原融合）和抗梅毒螺旋体苍白球菌抗体的测定法。
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10.发明授权Treponema pallidum fused antigen and assay for anti-treponema pallidum antibodies using the same fused antigen 失效梅毒螺旋体融合抗原，并使用相同的融合抗原测定抗梅毒螺旋体pallidum抗体公开(公告)号：US06248331B1
公开(公告)日：2001-06-19
申请号：US08773106
申请日：1996-12-26
IPC分类号： A61K3900
CPC分类号： C07K14/20 , C07K2319/00 , Y10S530/806 , Y10S530/82
摘要： A Treponema pallidum fused antigen in which at least two surface antigens of Treponema pallidum are fused and an assay for anti-Treponema pallidum antibodies, using the above Treponema pallidum fused antigen.
摘要翻译： 使用上述梅毒螺旋体融合抗原，梅毒螺旋体融合抗原（其中将梅毒螺旋体的至少两个表面抗原融合）和抗梅毒螺旋体苍白球菌抗体的测定法。
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