公开(公告)号：US5093081A

公开(公告)日：1992-03-03

申请号：US369332

申请日：1989-06-21

申请人： Yukio Sudo ,  Yoshihiro Ashihara ,  Toshikage Hiraoka ,  Isao Nishizono ,  Shigeki Kageyama ,  Tetsuji Tanimoto

发明人： Yukio Sudo ,  Yoshihiro Ashihara ,  Toshikage Hiraoka ,  Isao Nishizono ,  Shigeki Kageyama ,  Tetsuji Tanimoto

IPC分类号： G01N33/543

CPC分类号： G01N33/54386

摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-insoluble macromolecular substrate, and(B) an antibody, reacting with the ligand in the sample, conjugated with an enzyme capable of acting on the above water-insoluble macromolecular substrate.The above analytical substance further comprises,(C) a macromolecular substance which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct a highly sensitive and simple enzyme immunoassay.

公开(公告)号：US5603898A

公开(公告)日：1997-02-18

申请号：US993964

申请日：1992-12-17

申请人： Yoshihiro Ashihara ,  Yukio Sudo ,  Isao Nishizono ,  Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Shigeki Kageyama

发明人： Yoshihiro Ashihara ,  Yukio Sudo ,  Isao Nishizono ,  Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Shigeki Kageyama

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N21/00 ,  C07K16/00 ,  C12M1/14 ,  G01N33/53

CPC分类号： G01N33/581 ,  G01N33/54386

摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.

摘要翻译： 一种用于免疫测定的干式分析元件，其具有至少一个用于根据酶免疫测定法测量样品中的配体的透水层，其包含（A）水溶性大分子底物和（B）与抗体结合的酶 与能够作用于上述水溶性大分子底物的样品中的配体反应。 上述分析元素还包含（C）在上述干式分析元件的上述透水层中作为配体或其衍生物与高分子化合物的共轭物的高分子抗​​原。 本发明可以进行高灵敏度和简单的酶免疫测定。

公开(公告)号：US5158895A

公开(公告)日：1992-10-27

申请号：US677686

申请日：1991-03-29

申请人： Yoshihiro Ashihara ,  Isao Nishizono ,  Hidetaka Minakawa ,  Masahisa Okada ,  Yasusuke Sakurabayashi ,  Fumio Watanabe ,  Shin-ichi Wakana

发明人： Yoshihiro Ashihara ,  Isao Nishizono ,  Hidetaka Minakawa ,  Masahisa Okada ,  Yasusuke Sakurabayashi ,  Fumio Watanabe ,  Shin-ichi Wakana

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10

CPC分类号： G01N33/53 ,  G01N33/54326 ,  G01N35/02 ,  G01N2035/00534 ,  G01N2035/0465 ,  G01N2035/0498 ,  G01N35/0098 ,  G01N35/1079 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/119163

摘要： An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well containing solid phase material carrying antigen or antibody, and a second well containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film before use and the sealing film is broken when the cartridge is to be used. The cartridges are transported to a predetermined position on a steppingly movable reaction line and conveyed thereby at a predetermined interval. While the reaction line steps, a sample, labelled antigen or antibody contained in the second well and substrate, if necessary, are added to the first well and stirred at predetermined timings, and reactions between the sample and a reactive solution are measured under control of a control device having a memory storing operator selectable programs for various measuring methods.

摘要翻译： 自动免疫测定装置使用每个具有至少两个孔的盒，含有携带抗原或抗体的固相材料的第一孔和含标记化合物标记的抗体或抗原的第二孔。 在使用之前，孔可以用合适的密封膜密封，并且当使用盒时密封膜破裂。 药筒被输送到步进可移动的反应线上的预定位置，并以预定间隔被输送。 当反应线步骤时，如果需要，将第二阱和底物中包含的样品，标记的抗原或抗体加入到第一孔中并在预定的时间进行搅拌，并在样品和反应溶液之间的反应测量 具有存储器的控制装置，其存储用于各种测量方法的操作者可选程序。

公开(公告)号：US5290708A

公开(公告)日：1994-03-01

申请号：US915124

申请日：1992-07-17

申请人： Yoshihiro Ashihara ,  Isao Nishizono ,  Hidetaka Minakawa ,  Masahisa Okada ,  Yasusuke Sakurabayashi ,  Fumio Watanabe ,  Shin-ichi Wakana

发明人： Yoshihiro Ashihara ,  Isao Nishizono ,  Hidetaka Minakawa ,  Masahisa Okada ,  Yasusuke Sakurabayashi ,  Fumio Watanabe ,  Shin-ichi Wakana

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  C12Q1/00

CPC分类号： G01N33/53 ,  G01N33/54326 ,  G01N35/02 ,  G01N2035/00534 ,  G01N2035/0465 ,  G01N2035/0498 ,  G01N35/0098 ,  G01N35/1079 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/119163

摘要： An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well containing solid phase material carrying antigen or antibody, and a second well containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film before use and the sealing film is broken when the cartridge is to be used. The cartridges are transported to a predetermined position on a steppingly movable reaction line and conveyed thereby at a predetermined interval. While the reaction line steps, a sample, labelled antigen or antibody contained in the second well and substrate, if necessary, are added to the first well and stirred at predetermined timings, and reactions between the sample and a reactive solution are measured under control of a control device having a memory storing operator selectable programs for various measuring methods.

摘要翻译： 自动免疫测定装置使用每个具有至少两个孔的盒，含有携带抗原或抗体的固相材料的第一孔和含标记化合物标记的抗体或抗原的第二孔。 在使用之前，孔可以用合适的密封膜密封，并且当使用盒时密封膜破裂。 药筒被输送到步进可移动的反应线上的预定位置，并以预定间隔被输送。 当反应线步骤时，如果需要，将第二阱和底物中包含的样品，标记的抗原或抗体加入到第一孔中并在预定的时间进行搅拌，并在样品和反应溶液之间的反应测量 具有存储器的控制装置，其存储用于各种测量方法的操作者可选程序。

公开(公告)号：US5482839A

公开(公告)日：1996-01-09

申请号：US57503

申请日：1993-05-04

申请人： Yoshihiro Ashihara ,  Isao Nishizono ,  Hidetaka Minakawa ,  Masahisa Okada ,  Yasusuke Sakurabayashi ,  Fumio Watanabe ,  Shin-ichi Wakana

发明人： Yoshihiro Ashihara ,  Isao Nishizono ,  Hidetaka Minakawa ,  Masahisa Okada ,  Yasusuke Sakurabayashi ,  Fumio Watanabe ,  Shin-ichi Wakana

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N33/553

CPC分类号： G01N33/53 ,  G01N33/54326 ,  G01N35/02 ,  G01N2035/00534 ,  G01N2035/0465 ,  G01N2035/0498 ,  G01N35/0098 ,  G01N35/1079 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/119163

摘要： An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well of said wells containing solid phase material carrying antigen or antibody, a second well of said wells containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film before use and the sealing film is broken when the cartridge is to be used. The cartridges are transported to a predetermined position on a steppingly movable reaction line and conveyed thereby at a predetermined interval. While the reaction line steps, a sample, labelled antigen or antibody contained in the second well and substrate, if necessary, are added to the first well and stirred at predetermined timings and reaction between the sample and a reactive solution is measured under control of a control device having memory means storing operator selectable programs for various measuring methods.

摘要翻译： 自动免疫测定装置使用各自具有至少两个孔的柱，所述孔的第一孔包含携带抗原或抗体的固相材料，所述孔的第二孔含有用标记化合物标记的抗体或抗原。 在使用之前，孔可以用合适的密封膜密封，并且当使用盒时密封膜破裂。 药筒被输送到步进可移动的反应线上的预定位置，并以预定间隔被输送。 当反应线步骤时，如果需要，将第二阱和底物中包含的样品，标记的抗原或抗体加入到第一阱中并在预定的时间进行搅拌，并在样品和反应溶液之间进行反应，在 具有存储装置的控制装置存储用于各种测量方法的操作者可选程序。

公开(公告)号：US06534281B2

公开(公告)日：2003-03-18

申请号：US09878380

申请日：2001-06-12

申请人： Sachiko Kitajima ,  Yoshihiro Kurano ,  Kaoru Nakatsubo ,  Isao Nishizono

发明人： Sachiko Kitajima ,  Yoshihiro Kurano ,  Kaoru Nakatsubo ,  Isao Nishizono

IPC分类号： C07K1400

CPC分类号： G01N33/74 ,  G01N2333/62 ,  Y10S436/80 ,  Y10S436/809 ,  Y10S436/811 ,  Y10T436/25125

摘要： An immunoassay for selectively measuring human C-peptide as well as a kit therefor is disclosed. In the method, human C-peptide contained in a sample, a first anti-human C-peptide antibody, and a second anti-human C-peptide antibody which is immobilized on a solid support are reacted to form an immune complex among these three components. The formed immune complex is separated from the non-reacted antibodies and sample; and then the separated immune complex is quantified. The first antibody recognizes an epitope existing in the region from 1st to 16th amino acid residue from the N-terminal of the human C-peptide, and the second antibody recognizes an epitope existing in the region from 1st to 16th amino acid residue from the N-terminal of human C-peptide; with the proviso that the first and second antibodies do not recognize the same epitope so that they can simultaneously bind to said human C-peptide.