Dry-type analytical element for immunoassay
    1.
    发明授权
    Dry-type analytical element for immunoassay 失效
    用于免疫测定的干式分析元件

    公开(公告)号:US5603898A

    公开(公告)日:1997-02-18

    申请号:US993964

    申请日:1992-12-17

    CPC分类号: G01N33/581 G01N33/54386

    摘要: A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.

    摘要翻译: 一种用于免疫测定的干式分析元件,其具有至少一个用于根据酶免疫测定法测量样品中的配体的透水层,其包含(A)水溶性大分子底物和(B)与抗体结合的酶 与能够作用于上述水溶性大分子底物的样品中的配体反应。 上述分析元素还包含(C)在上述干式分析元件的上述透水层中作为配体或其衍生物与高分子化合物的共轭物的高分子抗​​原。 本发明可以进行高灵敏度和简单的酶免疫测定。

    Immunoassay element and process for immunoassay
    4.
    发明授权
    Immunoassay element and process for immunoassay 失效
    免疫测定元件和免疫测定方法

    公开(公告)号:US06589754B1

    公开(公告)日:2003-07-08

    申请号:US08946685

    申请日:1997-10-08

    IPC分类号: G01N3353

    CPC分类号: G01N33/54386

    摘要: An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.

    摘要翻译: 一种用于通过测定由高分子聚合物抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析大分子抗原的免疫测定元件。 免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 还提供了通过使用免疫测定元件定量分析样品中的大分子抗原的方法。

    Chemiluminescence enhancer
    8.
    发明授权
    Chemiluminescence enhancer 有权
    化学发光增强剂

    公开(公告)号:US07276343B2

    公开(公告)日:2007-10-02

    申请号:US10518586

    申请日:2003-06-24

    摘要: The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

    摘要翻译: 本发明提供一种化学发光增强剂,其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂,其用于固相免疫测定中的信号检测,所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上,该水溶液由水溶性大分子季铵盐,季锍盐或季鏻 盐,以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射,其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理,以及化学发光 方法和使用化学发光增强剂的试剂盒。

    Chemiluminescence enhancer
    9.
    发明申请

    公开(公告)号:US20070059786A1

    公开(公告)日:2007-03-15

    申请号:US11447839

    申请日:2006-06-07

    IPC分类号: G01N33/551 C07D305/02

    摘要: The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

    Chemiluminescence enhancer
    10.
    发明申请
    Chemiluminescence enhancer 有权
    化学发光增强剂

    公开(公告)号:US20050239149A1

    公开(公告)日:2005-10-27

    申请号:US10518586

    申请日:2003-06-24

    摘要: The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

    摘要翻译: 本发明提供一种化学发光增强剂,其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂,其用于固相免疫测定中的信号检测,所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上,该水溶液由水溶性大分子季铵盐,季锍盐或季鏻 盐,以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射,其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理和化学发光 方法和使用化学发光增强剂的试剂盒。