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公开(公告)号：US06589754B1
公开(公告)日：2003-07-08
申请号：US08946685
申请日：1997-10-08
申请人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
发明人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
IPC分类号： G01N3353
CPC分类号： G01N33/54386
摘要： An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.
摘要翻译： 一种用于通过测定由高分子聚合物抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析大分子抗原的免疫测定元件。 免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 还提供了通过使用免疫测定元件定量分析样品中的大分子抗原的方法。
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公开(公告)号：US5569589A
公开(公告)日：1996-10-29
申请号：US163405
申请日：1993-12-07
申请人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Naofumi Hora , Yoshihiro Ashihara , Yukio Sudo , Toshihiro Mori
发明人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Naofumi Hora , Yoshihiro Ashihara , Yukio Sudo , Toshihiro Mori
IPC分类号： G01N31/22 , G01N33/542 , G01N33/543 , G01N33/53
CPC分类号： G01N33/54386 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.
摘要翻译： 一种免疫测定元件，用于通过测定配体，配体与高分子化合物的连接产物和酶标记的抗体之间的反应引起的酶活性的变化来定量分析配体。 免疫测定元件包括含有在酶存在下形成可扩散材料的非扩散性底物的底物层和含有用于进一步碎裂非可弥散材料的碎裂酶的试剂层。 还提供了通过使用免疫测定元件定量分析样品中所含的配体的方法。
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公开(公告)号：US5093081A
公开(公告)日：1992-03-03
申请号：US369332
申请日：1989-06-21
申请人： Yukio Sudo , Yoshihiro Ashihara , Toshikage Hiraoka , Isao Nishizono , Shigeki Kageyama , Tetsuji Tanimoto
发明人： Yukio Sudo , Yoshihiro Ashihara , Toshikage Hiraoka , Isao Nishizono , Shigeki Kageyama , Tetsuji Tanimoto
IPC分类号： G01N33/543
CPC分类号： G01N33/54386
摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-insoluble macromolecular substrate, and(B) an antibody, reacting with the ligand in the sample, conjugated with an enzyme capable of acting on the above water-insoluble macromolecular substrate.The above analytical substance further comprises,(C) a macromolecular substance which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct a highly sensitive and simple enzyme immunoassay.
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公开(公告)号：US5603898A
公开(公告)日：1997-02-18
申请号：US993964
申请日：1992-12-17
申请人： Yoshihiro Ashihara , Yukio Sudo , Isao Nishizono , Toshikage Hiraoka , Tetsuji Tanimoto , Shigeki Kageyama
发明人： Yoshihiro Ashihara , Yukio Sudo , Isao Nishizono , Toshikage Hiraoka , Tetsuji Tanimoto , Shigeki Kageyama
CPC分类号： G01N33/581 , G01N33/54386
摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.
摘要翻译： 一种用于免疫测定的干式分析元件，其具有至少一个用于根据酶免疫测定法测量样品中的配体的透水层，其包含（A）水溶性大分子底物和（B）与抗体结合的酶 与能够作用于上述水溶性大分子底物的样品中的配体反应。 上述分析元素还包含（C）在上述干式分析元件的上述透水层中作为配体或其衍生物与高分子化合物的共轭物的高分子抗原。 本发明可以进行高灵敏度和简单的酶免疫测定。
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公开(公告)号：US07276343B2
公开(公告)日：2007-10-02
申请号：US10518586
申请日：2003-06-24
IPC分类号： G01N33/53 , G01N33/542 , G01N33/543 , G01N33/553 , G01N33/566 , G01N21/75 , G01N21/76 , C12Q1/00 , C12N9/00 , C09K3/00
CPC分类号： G01N33/533 , G01N21/76 , G01N33/532
摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.
摘要翻译： 本发明提供一种化学发光增强剂，其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂，其用于固相免疫测定中的信号检测，所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上，该水溶液由水溶性大分子季铵盐，季锍盐或季鏻 盐，以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射，其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理，以及化学发光 方法和使用化学发光增强剂的试剂盒。
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公开(公告)号：US20070059786A1
公开(公告)日：2007-03-15
申请号：US11447839
申请日：2006-06-07
IPC分类号： G01N33/551 , C07D305/02
CPC分类号： G01N33/533 , G01N21/76 , G01N33/532
摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.
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公开(公告)号：US20050239149A1
公开(公告)日：2005-10-27
申请号：US10518586
申请日：2003-06-24
IPC分类号： G01N21/76 , G01N33/532 , G01N33/533 , G01N33/53 , G01N33/537 , G01N33/543 , C07D35/02
CPC分类号： G01N33/533 , G01N21/76 , G01N33/532
摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.
摘要翻译： 本发明提供一种化学发光增强剂，其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂，其用于固相免疫测定中的信号检测，所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上，该水溶液由水溶性大分子季铵盐，季锍盐或季鏻 盐，以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射，其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理和化学发光 方法和使用化学发光增强剂的试剂盒。
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公开(公告)号：US5094939A
公开(公告)日：1992-03-10
申请号：US382112
申请日：1989-07-19
CPC分类号： C12Q1/42 , C07F9/65512 , C12Q1/6816 , C12Q2334/00 , Y10S436/80 , Y10S436/805
摘要： A multi-pH chemiluminescence-based assay method comprising first-stage light production by the activation by cleavage of a phosphatase cleavable dioxetane derivative within a pH range optimal for enzyme catalysis, followed by the second-stage generation of increased light energy from the products of enzyme-catalyzed cleavage of the dioxetane derivative by the adjustment of the pH to a strongly alkaline pH optimal for the generation of light energy. The assay method is suitable for both solution phase and solid state assay formats. Light generation enhancers can be used further to increase the light yield.
摘要翻译： 一种基于多pH化学发光的测定方法，其包括通过在最适于酶催化的pH范围内切割磷酸酶裂解的二氧杂环丁烷衍生物进行激活的第一阶段光产生，随后是来自产物的第二阶段产生增加的光能 通过将pH调节至最适于产生光能的强碱性pH的二氧杂环丁烷衍生物的酶催化裂解。 测定方法适用于溶液相和固态分析方法。 光产生增强剂可以进一步用于增加光产率。
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公开(公告)号：US07771928B2
公开(公告)日：2010-08-10
申请号：US10229020
申请日：2002-08-28
IPC分类号： G01N33/53
CPC分类号： G01N33/558 , G01N33/571 , Y10S435/962 , Y10S435/97 , Y10S435/975 , Y10S436/81 , Y10S436/814 , Y10S436/817 , Y10S436/818 , Y10S436/906
摘要： Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.
摘要翻译： 公开了一种免疫测定装置，其包括标记物质点状部分和设置在其上的标本点部分，以及使用该装置的免疫测定方法。
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公开(公告)号：US5290708A
公开(公告)日：1994-03-01
申请号：US915124
申请日：1992-07-17
申请人： Yoshihiro Ashihara , Isao Nishizono , Hidetaka Minakawa , Masahisa Okada , Yasusuke Sakurabayashi , Fumio Watanabe , Shin-ichi Wakana
发明人： Yoshihiro Ashihara , Isao Nishizono , Hidetaka Minakawa , Masahisa Okada , Yasusuke Sakurabayashi , Fumio Watanabe , Shin-ichi Wakana
CPC分类号： G01N33/53 , G01N33/54326 , G01N35/02 , G01N2035/00534 , G01N2035/0465 , G01N2035/0498 , G01N35/0098 , G01N35/1079 , Y10S436/807 , Y10S436/809 , Y10T436/113332 , Y10T436/114998 , Y10T436/119163
摘要： An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well containing solid phase material carrying antigen or antibody, and a second well containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film before use and the sealing film is broken when the cartridge is to be used. The cartridges are transported to a predetermined position on a steppingly movable reaction line and conveyed thereby at a predetermined interval. While the reaction line steps, a sample, labelled antigen or antibody contained in the second well and substrate, if necessary, are added to the first well and stirred at predetermined timings, and reactions between the sample and a reactive solution are measured under control of a control device having a memory storing operator selectable programs for various measuring methods.
摘要翻译： 自动免疫测定装置使用每个具有至少两个孔的盒，含有携带抗原或抗体的固相材料的第一孔和含标记化合物标记的抗体或抗原的第二孔。 在使用之前，孔可以用合适的密封膜密封，并且当使用盒时密封膜破裂。 药筒被输送到步进可移动的反应线上的预定位置，并以预定间隔被输送。 当反应线步骤时，如果需要，将第二阱和底物中包含的样品，标记的抗原或抗体加入到第一孔中并在预定的时间进行搅拌，并在样品和反应溶液之间的反应测量 具有存储器的控制装置，其存储用于各种测量方法的操作者可选程序。
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