摘要:
The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5′-end to 3′-end, and secretory producing the heterologous protein by coryneform bacteria.
摘要:
The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5′-end to 3′-end, and secretory producing the heterologous protein by coryneform bacteria.
摘要:
By culturing a methanol-assimilating bacterium which harbors a DNA construct which contains a promoter sequence that functions in the methanol-assimilating bacterium and a nucleotide sequence that encodes a polypeptide containing a signal sequence and an objective protein which is functionally connected to the promoter sequence, in a liquid medium containing methanol as a major carbon source, the bacterium is allowed to secrete the objective protein, and the secreted objective protein is recovered.
摘要:
A method for manufacturing sponge iron and an apparatus for charging in the method are disclosed. Iron oxide powder and reducing-agent powder are charged such that alternating layers of the iron oxide powder and the reducing-agent powder are formed and such that each of the layers is in the form of a helix, and then a reduction treatment is performed. The method has not only high reaction efficiency of a gas, high quality, and high productivity, but also the advantage for a production adjustment because the amount of charge can be adjusted without the limitation of a reduction time. The molar ratio of the carbon content in the reducing agent to the oxygen content in the iron oxide in the reaction container is preferably 1.1 or more.
摘要:
A method and apparatus for producing molten metal from powder state ore through smelting process to be performed in a shaft furnace, utilized the reducing material having grain size greater than that n-times of the gas flow velocity corresponding grain size to charge from the top of a shaft furnace for forming fluidized bed at the upper section of the furnace and a reducing material filled section below the fluidized bed. The method and apparatus for smelting the powder state ore also takes the reducing material having smaller grain size to be blown into the furnace through tuyeres.
摘要:
A RAM the capacity of which is to be detected is constituted by 2.sup.N memory blocks each having a predetermined memory capacity. An address signal representing an existing memory capacity can be fed to the RAM. An address controller designates a predetermined address for each block of the RAM. A code write section sequentially writes a detection code at the predetermined address in each block of the RAM. A code readout section reads out the detection code from the predetermined address of the first block of the RAM designated by the address controller. A code comparator sequentially compares the detection code read out by the code readout section with a detection code last written by the code write section, so as to detect whether there is a coincidence therebetween, and, when a coincidence is obtained, the comparator is able to detect the capacity of the entire RAM based on the coincident detection code.
摘要:
A phase detector is disclosed in which means are provided for sensing when two AC signals being subjected to phase difference measurement become close in phase, within a predetermined range, and for inverting one of these signals in phase when such a close phase relationship is sensed. The phase difference between the phase inverted signal and the other signal is then measured, and compensation is applied to the measured results such as to compensate for the effects of the phase inversion operation. Insensitivity and "hunting" effects which occur with prior art phase detectors in the region of zero phase difference are thereby eliminated, and an output signal can be obtained which varies in a smooth and continuous manner with variations in the phase difference between the two signals under measurement, over a 360.degree. range.
摘要:
In a multiprocessory system comprising a plurality of CPUs interconnected by a common bus, means are provided whereby the CPUs are periodically and cyclically enabled to access the bus. Data transfer from one CPU to another is performed by first storing the data into a main memory connected to the bus, then transferring the data from main memory to the destination CPU when the latter is enabled to utilize the bus and is in a condition to accept the data. Means can also be provided whereby, when data must be immediately transferred from one CPU to another, the sending CPU stores the data in main memory, generates signals whereby the destination CPU is given use of the bus, and generates an interrupt which causes transfer of the stored data into the destination CPU.
摘要:
A signal-measuring apparatus for measuring a level of a signal to be measured includes a reference signal oscillator, a variable local signal oscillator, a heterodyne type mixer, a bandpass filter and automatic detuning correction means. In the automatic detuning correction means, there are provided a peak value memory, a tracking circuit and a detuning-correction calculating circuit. When a filter output is obtained by passing the reference signal through the bandpass filter, a peak value is acquired by varying the oscillator frequency of the local signal. This peak value is stored in the peak value memory. Then, the signal to be measured is supplied to the bandpass filter to acquire another filter output. This filter output is calculated in the detuning correction calculating circuit based upon the above-described peak value as a reference value so as to correct the error caused by the filter detuning.
摘要:
An instruction code conversion unit, an information processing system provided with the instruction code conversion unit and an instruction code generation method for generating instruction codes which are converted by the instruction code conversion unit are described. The efficiency of coding of the program is improved by making use of an existing processor as selected is used without modification. An instruction code conversion unit performs conversion of the address of a native instruction code to the address of the corresponding compressed instruction code in a program memory by shifting the address of the native instruction code as outputted from the processor to the right by one bit.