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    Modified glucose dehydrogenase gene
    4.
    发明授权
    Modified glucose dehydrogenase gene 有权
    改良葡萄糖脱氢酶基因

    公开(公告)号：US08445221B2

    公开(公告)日：2013-05-21

    申请号：US12810213

    申请日：2008-12-26

    申请人： Michinari Honda ,  Ryo Takenaka ,  Fuminao Kobayashi

    发明人： Michinari Honda ,  Ryo Takenaka ,  Fuminao Kobayashi

    IPC分类号： C12Q1/54 ,  C12N9/00 ,  C12N9/02 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12Q1/32 ,  C12N9/0006 ,  C12Q1/006

    摘要： [Object] To provide an FAD-conjugated glucose dehydrogenase having a higher specificity for glucose.[Means for Resolution] A modified glucose dehydrogenase (GLD) which includes a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 37, 69, 72, 73, 76, 78, 102, 217, 228, 240, 356, 407, 424, 437, 527, and 530 in an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1, and has a decreased ratio of activity for xylose/activity for glucose as compared with the wild-type GLD; a polynucleotide encoding the modified GLD; a recombinant vector containing the polynucleotide; a transformed cell produced by using the recombinant vector; etc.

    摘要翻译： 提供对葡萄糖具有较高特异性的FAD-缀合的葡萄糖脱氢酶。 [解决方法]一种修饰的葡萄糖脱氢酶（GLD），其包括至少一个氨基酸残基的取代，所述氨基酸残基选自第37,69,72,73,76,78,102,217， 在由SEQ ID NO：1表示的野生型FAD-缀合的葡萄糖脱氢酶（GLD）的氨基酸序列中的228,240,356,407,424,473,527和530，并且具有降低的 与野生型GLD相比，葡萄糖的木糖/活性; 编码经修饰的GLD的多核苷酸; 含有多核苷酸的重组载体; 通过使用重组载体产生的转化细胞; 等等

    Operator guidance system and image forming apparatus incorporating same
    7.
    发明授权
    Operator guidance system and image forming apparatus incorporating same 有权
    操作者引导系统和包含其的图像形成装置

    公开(公告)号：US08970858B2

    公开(公告)日：2015-03-03

    申请号：US13678671

    申请日：2012-11-16

    申请人： Mami Akiyama ,  Yasuhiro Sagawa ,  Masaharu Furuya ,  Manabu Izumikawa ,  Mikio Ishibashi ,  Yoshio Hattori ,  So Ohta ,  Ryo Takenaka ,  Hiroaki Murakami

    发明人： Mami Akiyama ,  Yasuhiro Sagawa ,  Masaharu Furuya ,  Manabu Izumikawa ,  Mikio Ishibashi ,  Yoshio Hattori ,  So Ohta ,  Ryo Takenaka ,  Hiroaki Murakami

    IPC分类号： G06F3/12 ,  G03G15/00

    CPC分类号： G06F3/12 ,  G03G15/502 ,  G03G15/70

    摘要： An operator guidance system includes a detection unit, a control unit, and an operation indicator. The detection unit is disposed in an image forming apparatus to output a combination of detection signals, and includes one or more media sensors, and one or more opening sensors. The one or more media sensors are disposed along a media conveyance path to output a media detection signal. The one or more opening sensors are disposed adjacent to an openable structure to output an opening detection signal. The control unit is connected to the detection unit to determine guidance to be provided to an operator based on the combination of detection signals, and includes a memory and a controller. The operation indicator is connected to the control unit to indicate the identified operation to the operator.

    摘要翻译： 操作者引导系统包括检测单元，控制单元和操作指示器。 检测单元设置在图像形成装置中以输出检测信号的组合，并且包括一个或多个介质传感器和一个或多个打开传感器。 一个或多个介质传感器沿着介质传送路径布置以输出介质检测信号。 一个或多个打开传感器被布置成与可打开的结构相邻以输出打开检测信号。 控制单元连接到检测单元，以基于检测信号的组合确定要提供给操作者的指导，并且包括存储器和控制器。 操作指示器连接到控制单元以向操作者指示所识别的操作。

    Protein electron mediator
    8.
    发明授权
    Protein electron mediator 有权
    蛋白质电子介体

    公开(公告)号：US08716442B2

    公开(公告)日：2014-05-06

    申请号：US12857880

    申请日：2010-08-17

    申请人： Ryo Takenaka ,  Michinari Honda ,  Hironori Omura

    发明人： Ryo Takenaka ,  Michinari Honda ,  Hironori Omura

    IPC分类号： C07K14/00

    CPC分类号： H01M8/16 ,  A61B5/14532 ,  A61B5/1486 ,  C07K14/80 ,  C12N9/0006 ,  C12N15/62 ,  C12Q1/004 ,  Y02E60/527

    摘要： The problem to be resolved is to provide an electron mediator and a fusion body with high affinity with an enzyme, a measuring method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.The present invention relates to an electron mediator for glucose oxidoreductase comprising extracellular secretion type cytochrome, a fusion body in which the electron mediator is fused with glucose oxidoreductase, a composition for glucose measurement including the electron mediator or fusion body, a gene encoding a new extracellular secretion type cytochrome, and a measurement method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.

    摘要翻译： 要解决的问题是提供与酶具有高亲和力的电子介体和融合体，使用细胞外分泌型细胞色素的测定方法和酶，电极和传感器。 本发明涉及包含细胞外分泌型细胞色素的葡萄糖氧化还原酶的电子介体，其中电子介体与葡萄糖氧化还原酶融合的融合体，包括电子介体或融合体的葡萄糖测量用组合物，编码新的细胞外基因的基因 分泌型细胞色素，以及使用细胞外分泌型细胞色素和酶，电极和传感器的测定方法。

    Sheet thickness detecting device, feeding device, and image forming apparatus
    10.
    发明授权
    Sheet thickness detecting device, feeding device, and image forming apparatus 有权
    板厚检测装置，送料装置和成像装置

    公开(公告)号：US08794626B2

    公开(公告)日：2014-08-05

    申请号：US13756684

    申请日：2013-02-01

    申请人： Ryo Takenaka ,  Shingo Nishizaki ,  Yusuke Ozaki ,  Masashi Satoh ,  Hiroshi Adachi ,  Hiroshi Okamura ,  Naohiro Funada ,  Yuji Ikeda

    发明人： Ryo Takenaka ,  Shingo Nishizaki ,  Yusuke Ozaki ,  Masashi Satoh ,  Hiroshi Adachi ,  Hiroshi Okamura ,  Naohiro Funada ,  Yuji Ikeda

    IPC分类号： B65H7/12 ,  B65H7/02

    CPC分类号： B65H5/062 ,  B65H7/02 ,  B65H2404/144 ,  B65H2404/6111 ,  B65H2511/13 ,  B65H2511/224 ,  B65H2553/61 ,  B65H2220/01 ,  B65H2220/02 ,  B65H2220/03

    摘要： A sheet thickness detecting device for detecting a thickness of a sheet includes a fixed roller rotatably supported by a shaft so that the fixed roller faces the sheet; a movable roller rotatably provided so that the movable roller and the fixed roller sandwich and feed the sheet, and configured to be displaced in accordance with the thickness of the sandwiched sheet; a swing member rotatably supporting the movable roller and including a shaft so that the swing member is swung around the shaft, wherein the swing member further includes a detection target part formed in a manner that a distance between the detection target part and the shaft is greater than that between a position where the movable roller is supported by the swing member and the shaft; and a detecting unit detecting the displacement amount of the detection target part of the swing member.

    摘要翻译： 用于检测片材厚度的片材厚度检测装置包括由轴可旋转地支撑的固定辊，使得固定的辊面对片材; 可移动的辊，其可旋转地设置成使得所述可动辊和所述固定辊夹持并进给所述片材，并且被构造成根据所述夹层片材的厚度移位; 摆动构件，其可旋转地支撑所述可动辊并且包括轴，使得所述摆动构件围绕所述轴摆动，其中所述摆动构件还包括以使得所述检测对象部分和所述轴之间的距离更大的方式形成的检测目标部分 比可动辊被摆动构件支撑的位置与轴之间的位移大; 以及检测单元，检测摆动构件的检测目标部分的位移量。