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    Methods for the production of insulin in plants
    1.
    发明授权
    Methods for the production of insulin in plants 失效
    在植物中生产胰岛素的方法

    公开(公告)号：US07547821B2

    公开(公告)日：2009-06-16

    申请号：US10869040

    申请日：2004-06-17

    申请人： Maurice M. Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

    发明人： Maurice M. Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

    IPC分类号： C12N15/82 ,  A01H5/00 ,  C07H21/04

    CPC分类号： C07K14/62 ,  C12N15/8257

    摘要： Commercial production of human insulin can be affected via transgenic expression in plant seeds. Thus, levels of insulin accumulation exceeding 0.1% of total cellular protein can be achieved recombinantly, through expression of the insulin with a single-chain antibody as a fusion partner. Production in seeds offers flexibility in storage and shipment of insulin as a raw material, and insulin retains its activity upon extraction from stored seed. Further, the amount of biomass subjected to extraction is limited, due to the relatively low water content of plant seeds.

    摘要翻译： 人类胰岛素的商业生产可以通过植物种子中的转基因表达来影响。 因此，通过以单链抗体作为融合配偶体的胰岛素的表达，可以重组地实现超过总细胞蛋白质的0.1％的胰岛素积聚的水平。 种子生产提供了作为原料的胰岛素储存和运输的灵活性，胰岛素从储存的种子提取时保留其活性。 此外，由于植物种子的含水量相对较低，所以提取的生物质的量受到限制。

    Flax seed specific promoters
    2.
    发明授权
    Flax seed specific promoters 有权
    亚麻种子特异性启动子

    公开(公告)号：US07642346B2

    公开(公告)日：2010-01-05

    申请号：US10804219

    申请日：2004-03-19

    申请人： Sarita Chaudhary ,  Gijs Van Rooijen ,  Maurice M. Moloney ,  Surinder Singh

    发明人： Sarita Chaudhary ,  Gijs Van Rooijen ,  Maurice M. Moloney ,  Surinder Singh

    IPC分类号： C12N15/82

    CPC分类号： C07K14/415 ,  C12N15/8234

    摘要： Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.

    摘要翻译： 提供了亚麻籽中非天然基因表达和其他植物种子种子的新方法。 该方法涉及使用从亚麻获得的种子特异性启动子。 另外提供了新的亚麻种子特异性启动子，包含新亚麻种子特异性启动子，转基因植物细胞，转基因植物和含有新亚麻种子特异性启动子的转基因植物种子的嵌合核酸构建体。 启动子和方法可用于例如改变亚麻种子或其他植物种子中的种子油和蛋白质组成。

    Preparation of heterologous proteins on oil bodies
    5.
    发明授权
    Preparation of heterologous proteins on oil bodies 失效
    在油体上制备异源蛋白质

    公开(公告)号：US07666628B2

    公开(公告)日：2010-02-23

    申请号：US10763380

    申请日：2004-01-26

    申请人： Maurice M. Moloney ,  Gijs Van Rooijen

    发明人： Maurice M. Moloney ,  Gijs Van Rooijen

    IPC分类号： C12P21/02 ,  C12N15/09 ,  C12P21/04 ,  C07H21/04 ,  A07H5/00 ,  C12N15/82 ,  C12N15/87

    CPC分类号： C12N9/6483 ,  C07K14/415 ,  C07K14/545 ,  C07K14/61 ,  C07K14/8139 ,  C07K14/815 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/6429 ,  C12N15/8222 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8257 ,  C12Y304/21005 ,  C12Y304/23004

    摘要： The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.

    摘要翻译： 本发明涉及具有独特特征的称为油体蛋白基因的一类基因的用途。 这些特征的发现允许本发明用于生产重组蛋白质的方法，其中感兴趣的蛋白质可以容易地与其他宿主细胞组分分离。 本发明进一步举例说明了利用油体蛋白和油体基因在许多生物体，特别是植物种子中表达目标多肽的独特特征的方法。 所述多肽可以包括但不限于：种子储存蛋白，酶，生物活性肽，抗体等。 还可以修饰本发明以从非植物宿主细胞中回收与油体蛋白融合的重组多肽。 另外，本发明提供了一种使用与种子发芽期间释放的种子油体相关的重组蛋白的方法，用于表达能够保护来自病原体的幼苗的多肽。 最后，油体蛋白与油体的持续结合可以进一步利用，以开发生物学手段来产生可用于生物转化底物的新型固定化酶。

    Methods for the production of insulin in plants
    7.
    发明申请
    Methods for the production of insulin in plants 失效
    在植物中生产胰岛素的方法

    公开(公告)号：US20050039235A1

    公开(公告)日：2005-02-17

    申请号：US10869040

    申请日：2004-06-17

    申请人： Maurice Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

    发明人： Maurice Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

    IPC分类号： A01H1/00 ,  C07K14/62 ,  C12N15/82

    CPC分类号： C07K14/62 ,  C12N15/8257

    摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5′ to 3′ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

    摘要翻译： 描述了在植物中产生胰岛素的方法。 在一个实施方案中，本发明提供了一种用于在植物中表达胰岛素的方法，包括：（a）提供嵌合核酸构建体，其以5'至3'的转录方向包含可操作地连接的组分：（i）核酸 能够控制植物种子细胞中表达的序列; 和（ii）编码胰岛素多肽的核酸序列; （b）将嵌合核酸构建体引入植物细胞; 和（c）将植物细胞生长成能种植种子的成熟植物，其中种子表达胰岛素。

    Oil bodies and associated proteins as affinity matrices
    9.
    发明授权
    Oil bodies and associated proteins as affinity matrices 失效
    油体和相关蛋白质作为亲和基质

    公开(公告)号：US06509453B1

    公开(公告)日：2003-01-21

    申请号：US09319275

    申请日：1999-08-27

    申请人： Maurice Moloney ,  Joseph Boothe ,  Gijs Van Rooijen

    发明人： Maurice Moloney ,  Joseph Boothe ,  Gijs Van Rooijen

    IPC分类号： C07K100

    CPC分类号： C07H21/00 ,  B01D15/08 ,  B01D15/3804 ,  B01D15/3809 ,  C01G11/003 ,  C07K1/22 ,  C07K14/415 ,  C07K16/065 ,  C08B1/00 ,  C12N9/6429 ,  C12Y304/21005

    摘要： A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions

    摘要翻译： 描述了从混合物中分离靶分子的方法。 该方法使用油体及其相关蛋白作为所需靶分子的选择性，非共价结合的亲和基质。 油体蛋白质可以遗传融合到对所需靶分子具有特异性的配体。 天然油体蛋白质也可以与油体蛋白质特异性配体如抗体或油体结合蛋白结合使用。 该方法允许由于油体和水溶液之间的密度差而使所需目标分子分离和回收