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    Methods for screening for inhibitors of the transcription-enhancing activity of the X protein of hepatitis B virus
    1.
    发明授权
    Methods for screening for inhibitors of the transcription-enhancing activity of the X protein of hepatitis B virus 失效
    筛选乙型肝炎病毒X蛋白转录增强活性抑制剂的方法

    公开(公告)号：US06051373A

    公开(公告)日：2000-04-18

    申请号：US351659

    申请日：1994-12-07

    申请人： Michael R. Green ,  Giovanni Perini ,  James Lillie

    发明人： Michael R. Green ,  Giovanni Perini ,  James Lillie

    IPC分类号： G01N33/576 ,  C07K14/02 ,  C12N15/00 ,  C12N15/09 ,  C12N15/67 ,  C12Q1/18 ,  C12Q1/68 ,  C12R1/92 ,  G01N33/15 ,  C12Q1/70 ,  G01N33/53 ,  G01N33/566

    CPC分类号： C07K14/005 ,  C12N15/67 ,  C12Q1/18 ,  C12N2730/10122

    摘要： Disclosed is a method for screening for inhibitors of Hepatitis B Virus pX activity. The method involves contacting a test compound with (I) the pX protein of HBV, (ii) a transcription factor comprising the bZIP domain, or fragments that comprise a minimal a bZIP domain, and (iii) an oligoduplex comprising a target DNA sequence of the transcription factor to form a test mixture. After incubating the test mixture under appropriate conditions and for a sufficient time to allow pX-mediated dimerization and DNA binding of the transcription factor to occur, the level of DNA binding of the transcription factor in each test mixture is determined. A test compound is considered to be any compound that causes a decrease in the level of DNA binding in the test mixture relative to the level of DNA binding in control mixtures.

    摘要翻译： 公开了筛选乙型肝炎病毒pX活性抑制剂的方法。 该方法包括使测试化合物与（I）HBV的pX蛋白质接触，（ii）包含bZIP结构域的转录因子或包含最小bZIP结构域的片段，和（iii）包含靶DNA DNA序列的寡偶联 转录因子形成试验混合物。 将试验混合物在合适的条件下孵育足够的时间以允许pX介导的二聚化和转录因子的DNA结合发生，确定每个测试混合物中转录因子的DNA结合水平。 被测化合物被认为是导致测试混合物中DNA结合水平相对于对照混合物中DNA结合水平降低的任何化合物。