公开(公告)号：US09127054B2

公开(公告)日：2015-09-08

申请号：US13992567

申请日：2011-12-05

申请人： Michimoto Kobayashi ,  Yoshinori Tanaka ,  Aiko Takayama ,  Satoko Kanamori ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

发明人： Michimoto Kobayashi ,  Yoshinori Tanaka ,  Aiko Takayama ,  Satoko Kanamori ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

IPC分类号： C07K16/18 ,  G01N33/577 ,  G01N33/574

CPC分类号： C07K16/18 ,  C07K2317/34 ,  G01N33/57446 ,  G01N33/577

摘要： An object of the present invention is to realize an antibody or a fragment thereof specifically recognizing a cofilin 1 protein, and a method for detecting and testing gastrointestinal cancer with high detection performance, which comprises performing immunoassay of the cofilin 1 protein using the antibody or a fragment thereof. An immunoassay of cofilin 1 protein is characterized by measuring cofilin 1 or a fragment thereof in a sample using 2 or more types of anti-cofilin 1 monoclonal antibody or fragments thereof specifically recognizing different peptide regions in the amino acid sequence constituting the cofilin 1 protein.

摘要翻译： 本发明的目的是实现特异性识别椰子蛋白1蛋白的抗体或其片段，以及检测和检测具有高检测性能的胃肠癌的方法，其包括使用抗体或免疫球蛋白进行免疫测定 片段。 使用两种或更多种类型的抗-cofilin1单克隆抗体或其片段特异性识别构成所述辅酶1蛋白的氨基酸序列中的不同肽区域的方法，对样品中的菲菲林1或其片段进行测定。

公开(公告)号：US20130288277A1

公开(公告)日：2013-10-31

申请号：US13992567

申请日：2011-12-05

申请人： Michimoto Kobayashi ,  Yoshinori Tanaka ,  Aiko Takayama ,  Satoko Kanamori ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

发明人： Michimoto Kobayashi ,  Yoshinori Tanaka ,  Aiko Takayama ,  Satoko Kanamori ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

IPC分类号： C07K16/18 ,  G01N33/577

CPC分类号： C07K16/18 ,  C07K2317/34 ,  G01N33/57446 ,  G01N33/577

摘要： An object of the present invention is to realize an antibody or a fragment thereof specifically recognizing a cofilin 1 protein, and a method for detecting and testing gastrointestinal cancer with high detection performance, which comprises performing immunoassay of the cofilin 1 protein using the antibody or a fragment thereof. An immunoassay of cofilin 1 protein is characterized by measuring cofilin 1 or a fragment thereof in a sample using 2 or more types of anti-cofilin 1 monoclonal antibody or fragments thereof specifically recognizing different peptide regions in the amino acid sequence constituting the cofilin 1 protein.

摘要翻译： 本发明的目的是实现特异性识别椰子蛋白1蛋白的抗体或其片段，以及检测和检测具有高检测性能的胃肠癌的方法，其包括使用抗体或免疫球蛋白进行免疫测定 片段。 使用两种或更多种类型的抗-cofilin1单克隆抗体或其片段特异性识别构成所述辅酶1蛋白的氨基酸序列中的不同肽区域的方法，对样品中的菲菲林1或其片段进行测定。

公开(公告)号：US20120329074A1

公开(公告)日：2012-12-27

申请号：US13582316

申请日：2011-03-03

申请人： Yoshinori Tanaka ,  Satoko Kanamori ,  Michimoto Kobayashi ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

发明人： Yoshinori Tanaka ,  Satoko Kanamori ,  Michimoto Kobayashi ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

IPC分类号： G01N33/574

CPC分类号： G01N33/57446 ,  G01N2333/4712

摘要： It is intended to provide a method for detecting gastric cancer, which is low invasive to a human test subject and has high detection sensitivity and accuracy. The present invention provides a method comprising measuring in vitro the amount of COTL1 protein, a variant thereof, and/or a fragment thereof in a body fluid sample derived from a human test subject, and detecting the presence or absence of gastric cancer affecting the test subject on the basis of the amount, and a kit for gastric cancer diagnosis comprising an antibody capable of specifically binding to the protein.

摘要翻译： 目的在于提供一种检测对人体检测对象侵袭性低，检测灵敏度高，准确度高的胃癌的方法。 本发明提供了一种方法，其包括在源自人类受检者的体液样品中体外测量COTL1蛋白，其变体和/或其片段的量，并检测影响测试的胃癌的存在或不存在 基于该量的受试者，以及包含能够特异性结合蛋白质的抗体的胃癌诊断试剂盒。

公开(公告)号：US20130011865A1

公开(公告)日：2013-01-10

申请号：US13582382

申请日：2011-03-03

申请人： Michimoto Kobayashi ,  Yoshinori Tanaka ,  Satoko Kanamori ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

发明人： Michimoto Kobayashi ,  Yoshinori Tanaka ,  Satoko Kanamori ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

IPC分类号： G01N33/574 ,  G01N21/64 ,  C07K16/18

CPC分类号： G01N33/57446 ,  C07K14/4702 ,  G01N33/57488

摘要： It is intended to provide a method for detecting gastric cancer, which is low invasive to a human test subject and has high detection sensitivity and accuracy. The present invention provides a method comprising measuring in vitro the amount of Cofilin 1 protein, a variant thereof, and/or a fragment thereof in a body fluid sample derived from a human test subject, and detecting the presence or absence of gastric cancer affecting the test subject on the basis of the amount, and a kit for gastric cancer diagnosis comprising an antibody capable of specifically binding to the protein.

摘要翻译： 目的在于提供一种检测对人体检测对象侵袭性低，检测灵敏度高，准确度高的胃癌的方法。 本发明提供了一种方法，其包括在体外测定源自人类受检者的体液样品中的Cofilin1蛋白，其变体和/或其片段的量，并检测是否存在影响 基于该量的测试对象，以及包含能够特异性结合蛋白质的抗体的胃癌诊断试剂盒。

公开(公告)号：US09081013B2

公开(公告)日：2015-07-14

申请号：US13582316

申请日：2011-03-03

申请人： Yoshinori Tanaka ,  Satoko Kanamori ,  Michimoto Kobayashi ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

发明人： Yoshinori Tanaka ,  Satoko Kanamori ,  Michimoto Kobayashi ,  Giman Jung ,  Yoshiharu Sakai ,  Hiroshi Okabe

IPC分类号： G01N33/53 ,  G01N33/574

CPC分类号： G01N33/57446 ,  G01N2333/4712

摘要： It is intended to provide a method for detecting gastric cancer, which is low invasive to a human test subject and has high detection sensitivity and accuracy. The present invention provides a method comprising measuring in vitro the amount of COTL1 protein, a variant thereof, and/or a fragment thereof in a body fluid sample derived from a human test subject, and detecting the presence or absence of gastric cancer affecting the test subject on the basis of the amount, and a kit for gastric cancer diagnosis comprising an antibody capable of specifically binding to the protein.

摘要翻译： 目的在于提供一种检测对人体检测对象侵袭性低，检测灵敏度高，准确度高的胃癌的方法。 本发明提供了一种方法，其包括在源自人类受检者的体液样品中体外测量COTL1蛋白，其变体和/或其片段的量，并检测影响测试的胃癌的存在或不存在 基于该量的受试者，以及包含能够特异性结合蛋白质的抗体的胃癌诊断试剂盒。

公开(公告)号：US09309312B2

公开(公告)日：2016-04-12

申请号：US13126877

申请日：2009-10-29

申请人： Satoko Kanamori ,  Giman Jung ,  Yoshinori Tanaka ,  Aiko Takayama

发明人： Satoko Kanamori ,  Giman Jung ,  Yoshinori Tanaka ,  Aiko Takayama

IPC分类号： C07K16/24 ,  G01N33/68 ,  G01N33/543 ,  G01N33/563 ,  A61K39/00

CPC分类号： C07K16/24 ,  A61K2039/505 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/76 ,  G01N33/543 ,  G01N33/6857 ,  G01N33/6863 ,  G01N2333/522

摘要： An object of the present invention is to detect a human CXCL1 protein with high sensitivity. An immunoassay method is provided for a human CXCL1 protein, by which human CXCL1 or a fragment thereof in a sample is measured using two or more types of anti-human CXCL1 monoclonal antibodies or fragments thereof, wherein: each of the two or more types of anti-human CXCL1 monoclonal antibodies or fragments thereof specifically recognizes any one of sequence regions of the amino acid sequences shown in SEQ ID NOS: 1-3, which are partial sequences of the amino acid sequence composing a human CXCL1 protein; and the two or more types of anti-human CXCL1 monoclonal antibodies or fragments thereof specifically recognize sequence regions that differ from each other. Monoclonal antibodies or fragments thereof are provided, each of which specifically recognizes any one sequence region of the amino acid sequences shown in SEQ ID NOS: 1-3 and has a new amino acid sequence.

摘要翻译： 本发明的目的是高灵敏度地检测人CXCL1蛋白。 提供了人CXCL1蛋白的免疫测定方法，通过该方法，使用两种或更多种抗人CXCL1单克隆抗体或其片段测量样品中的人CXCL1或其片段，其中：所述两种或更多种类型的 抗人CXCL1单克隆抗体或其片段特异性识别作为构成人CXCL1蛋白的氨基酸序列的部分序列的SEQ ID NO：1-3所示的氨基酸序列的任何一个序列区域; 两种以上抗人CXCL1单克隆抗体或其片段特异性识别彼此不同的序列区。 提供了单克隆抗体或其片段，其各自特异性识别SEQ ID NO：1-3所示的氨基酸序列的任何一个序列区，并具有新的氨基酸序列。

公开(公告)号：US20110287444A1

公开(公告)日：2011-11-24

申请号：US13126877

申请日：2009-10-29

申请人： Satoko Kanamori ,  Giman Jung ,  Yoshinori Tanaka ,  Aiko Takayama

发明人： Satoko Kanamori ,  Giman Jung ,  Yoshinori Tanaka ,  Aiko Takayama

IPC分类号： G01N33/577 ,  C07K16/24

CPC分类号： C07K16/24 ,  A61K2039/505 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/76 ,  G01N33/543 ,  G01N33/6857 ,  G01N33/6863 ,  G01N2333/522

摘要： An object of the present invention is to detect a human CXCL1 protein with high sensitivity. An immunoassay method is provided for a human CXCL1 protein, by which human CXCL1 or a fragment thereof in a sample is measured using two or more types of anti-human CXCL1 monoclonal antibodies or fragments thereof, wherein: each of the two or more types of anti-human CXCL1 monoclonal antibodies or fragments thereof specifically recognizes any one of sequence regions of the amino acid sequences shown in SEQ ID NOS: 1-3, which are partial sequences of the amino acid sequence composing a human CXCL1 protein; and the two or more types of anti-human CXCL1 monoclonal antibodies or fragments thereof specifically recognize sequence regions that differ from each other. Monoclonal antibodies or fragments thereof are provided, each of which specifically recognizes any one sequence region of the amino acid sequences shown in SEQ ID NOS: 1-3 and has a new amino acid sequence.

摘要翻译： 本发明的目的是高灵敏度地检测人CXCL1蛋白。 提供了人CXCL1蛋白的免疫测定方法，通过该方法，使用两种或更多种抗人CXCL1单克隆抗体或其片段测量样品中的人CXCL1或其片段，其中：所述两种或更多种类型的 抗人CXCL1单克隆抗体或其片段特异性识别作为构成人CXCL1蛋白的氨基酸序列的部分序列的SEQ ID NO：1-3所示的氨基酸序列的任何一个序列区域; 两种以上抗人CXCL1单克隆抗体或其片段特异性识别彼此不同的序列区。 提供了单克隆抗体或其片段，其各自特异性识别SEQ ID NO：1-3所示的氨基酸序列的任何一个序列区，并具有新的氨基酸序列。

公开(公告)号：US20130280773A1

公开(公告)日：2013-10-24

申请号：US13996241

申请日：2011-12-21

申请人： Norihiro Takeuchi ,  Jihoon Cheon ,  Makoto Nishida ,  Satoko Kanamori ,  Atsushi Kobayashi

发明人： Norihiro Takeuchi ,  Jihoon Cheon ,  Makoto Nishida ,  Satoko Kanamori ,  Atsushi Kobayashi

IPC分类号： C12P7/56

CPC分类号： C12P7/56 ,  C12M29/04 ,  C12M33/14

摘要： A method of producing a chemical includes a fermentation step that converts a fermentation feedstock, through fermentation by culturing a microorganism or culture cells, into a fermented liquid containing the chemical, and a membrane separation step that collects the chemical, as a filtrate, from the fermented liquid with the use of two or more separation membrane modules, and returning the non-filtered liquid to the fermented liquid, wherein in the membrane separation step, timing of the filtration-stop treatment for each separation membrane module is controlled when an intermittent filtration operation including alternately repeating a filtration treatment and a filtration-stop treatment is performed with plural separation membrane modules.

摘要翻译： 制造化学品的方法包括发酵步骤，其通过将微生物或培养细胞培养发酵成发酵液，将发酵原料转化成含有该化学品的发酵液，以及膜分离步骤，将滤液中的化学物质从 使用两个或更多个分离膜组件的发酵液体，并将未过滤的液体返回到发酵液中，其中在膜分离步骤中，当间歇过滤时，控制每个分离膜组件的过滤停止处理的时间 用多个分离膜组件进行交替重复过滤处理和过滤停止处理的操作。

公开(公告)号：US09365876B2

公开(公告)日：2016-06-14

申请号：US13992443

申请日：2011-12-08

申请人： Satoko Kanamori ,  Jihoon Cheon ,  Takashi Mimitsuka ,  Norihiro Takeuchi ,  Makoto Nishida ,  Yuji Tanaka

发明人： Satoko Kanamori ,  Jihoon Cheon ,  Takashi Mimitsuka ,  Norihiro Takeuchi ,  Makoto Nishida ,  Yuji Tanaka

IPC分类号： C12P1/00 ,  C12P13/04 ,  C12P13/08 ,  C12P7/40 ,  C12P7/56 ,  C12P13/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/34

CPC分类号： C12P13/08 ,  C12M29/02 ,  C12M29/18 ,  C12M33/14 ,  C12M41/40 ,  C12M47/10 ,  C12P7/40 ,  C12P7/56 ,  C12P13/001 ,  C12P13/04

摘要： A method produces a chemical through continuous fermentation including: (a) culturing a cell in a culture medium in a fermentor to ferment a feedstock to produce a chemical; (b) conducting filtration of the culture medium with a separation membrane module; (c) separating a permeate containing the chemical from the culture medium while retaining a non-permeated liquid in the fermentor, and (d) supplying a gas from at least one of a lower portion of the separation membrane module and a pipe communicating between the fermentor and the separation membrane module to adjust a gas linear velocity in the separation membrane module to 0.15 cm/s to 70 cm/s while supplying the separation membrane module with a liquid.

摘要翻译： 方法通过连续发酵生产化学品，包括：（a）在发酵罐中培养培养基中的细胞以发酵原料以生产化学品; （b）用分离膜组件对培养基进行过滤; （c）将含有化学物质的渗透物从培养基中分离，同时将不渗透的液体保持在发酵罐中，和（d）从分离膜组件的下部中的至少一个供给气体， 发酵罐和分离膜组件，将分离膜组件中的气体线速度调节至0.15厘米/秒至70厘米/秒，同时向分离膜组件供应液体。

公开(公告)号：US20140308712A1

公开(公告)日：2014-10-16

申请号：US13991703

申请日：2011-12-07

申请人： Masayuki Hanakawa ,  Satoko Kanamori ,  Hiroyuki Kurihara ,  Norihiro Takeuchi ,  Atsushi Minamino

发明人： Masayuki Hanakawa ,  Satoko Kanamori ,  Hiroyuki Kurihara ,  Norihiro Takeuchi ,  Atsushi Minamino

IPC分类号： C13K1/04

CPC分类号： C13K1/04 ,  B01D61/022 ,  B01D61/025 ,  B01D61/027 ,  B01D61/142 ,  B01D61/145 ,  B01D61/147 ,  B01D61/16 ,  B01D61/58 ,  B01D65/02 ,  B01D2311/06 ,  B01D2311/25 ,  B01D2321/12 ,  C12P19/02 ,  C12P19/14 ,  C13K13/002

摘要： A method produces a concentrated aqueous sugar solution using a cellulose-containing biomass as a raw material, including: (1) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution; (2) filtering the aqueous sugar solution obtained in (1) through a microfiltration membrane and/or an ultrafiltration membrane, and recovering an aqueous sugar solution from the permeate side; and (3) filtering the aqueous sugar solution obtained in (2) through a nanofiltration membrane and/or a reverse osmosis membrane, recovering a permeate from the permeate side and recovering a concentrated aqueous sugar solution from the feed side; wherein at least a part of the permeate from the nanofiltration membrane and/or the reverse osmosis membrane is used as a washing liquid in (1) and/or (2).

摘要翻译： 一种方法使用含纤维素的生物质作为原料产生浓缩的糖水溶液，其包括：（1）水解含纤维素的生物质以产生糖水溶液; （2）通过微滤膜和/或超滤膜过滤由（1）得到的糖水溶液，从渗透侧回收糖溶液; （3）通过纳滤膜和/或反渗透膜过滤（2）得到的糖水溶液，从渗透侧回收渗透物质，从进料侧回收浓缩的糖水溶液; 其中，在（1）和/或（2）中使用来自所述纳滤膜和/或反渗透膜的至少一部分渗透物作为洗涤液。