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35 条记录 (耗时 0.051 秒)

    PORTABLE CONSOLE, WORKSTATION AND RADIOGRAPHY SYSTEM
    1.
    发明申请
    PORTABLE CONSOLE, WORKSTATION AND RADIOGRAPHY SYSTEM 审中-公开
    便利控制台，工作台和放射系统

    公开(公告)号：US20130232432A1

    公开(公告)日：2013-09-05

    申请号：US13600413

    申请日：2012-08-31

    申请人： Min Liu ,  Lei Jia ,  Li Shao ,  Yi Yan ,  Siyu Huang

    发明人： Min Liu ,  Lei Jia ,  Li Shao ,  Yi Yan ,  Siyu Huang

    IPC分类号： G06F19/00

    CPC分类号： G06F19/00 ,  A61B6/548 ,  G16H40/63

    摘要： A portable console communicates with a workstation of a radiography apparatus to allow a user to control the radiography apparatus via the portable console, the portable console comprising a user interface representing module configured to provide a user operation interface whereby the user inputs a user request, and configured to present a result returned from the workstation to the user, and a user interface service logical module configured to perform a protocol encapsulation and conversion on the user request according to a predefined communication protocol, to send the user request to the workstation, to receive the result returned from the workstation, and to perform a protocol conversion on the received result before sending the result to the user interface representing module.

    摘要翻译： 便携式控制台与射线照相设备的工作站进行通信，以允许用户经由便携式控制台来控制放射摄影装置，便携式控制台包括用户接口，其代表模块，该模块被配置为提供用户操作界面，由此用户输入用户请求;以及 被配置为向用户呈现从工作站返回的结果;以及用户接口服务逻辑模块，被配置为根据预定义的通信协议对用户请求执行协议封装和转换，以将用户请求发送到工作站，以接收 从工作站返回的结果，并且在将结果发送到表示模块的用户界面之前对接收到的结果进行协议转换。

    Recombinant polymerases for improved single molecule sequencing
    2.
    发明授权
    Recombinant polymerases for improved single molecule sequencing 有权
    用于改进单分子测序的重组聚合酶

    公开(公告)号：US08999676B2

    公开(公告)日：2015-04-07

    申请号：US13176697

    申请日：2011-07-05

    申请人： Robin Emig ,  Lei Jia ,  Satwik Kamtekar ,  Erik Miller ,  Colleen Cutcliffe ,  Walter Lee

    发明人： Robin Emig ,  Lei Jia ,  Satwik Kamtekar ,  Erik Miller ,  Colleen Cutcliffe ,  Walter Lee

    IPC分类号： C12N15/54 ,  C12N9/00 ,  C12N9/12 ,  C12Q1/68

    CPC分类号： C12N9/1252 ,  C12N9/1241 ,  C12P19/34 ,  C12Q1/6869

    摘要： Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

    摘要翻译： 提供了包含重组DNA聚合酶的组合物，其包括赋予聚合酶修饰性质以增强单分子测序的氨基酸取代，插入，缺失和/或外源特征。 这种性质可以包括增强的金属离子配位，降低的核酸外切酶活性，在聚合酶动力学循环的一个或多个步骤中降低的反应速率，降低的支化部分，改变的辅因子选择性，增加的产率，增加的热稳定性，增加的准确度，增加的速度，增加的读长度， 等等。 还提供了编码具有上述表型的聚合酶的核酸，以及使用这种聚合酶制备DNA或顺序DNA模板的方法。

    Sequencing methods using enzyme conformation
    3.
    发明授权
    Sequencing methods using enzyme conformation 有权
    使用酶构象的测序方法

    公开(公告)号：US08911972B2

    公开(公告)日：2014-12-16

    申请号：US12967507

    申请日：2010-12-14

    申请人： Mark Chaisson ,  Sonya Clark ,  Christopher Silk ,  John Lyle ,  Lei Jia ,  Keith Bjornson

    发明人： Mark Chaisson ,  Sonya Clark ,  Christopher Silk ,  John Lyle ,  Lei Jia ,  Keith Bjornson

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2521/101 ,  C12Q2561/113 ,  C12Q2563/107

    摘要： Systems and methods are provided for single-molecule sequencing of template nucleic acids in which the signal from a label attached to a polymerase enzyme is used to monitor conformational changes in the polymerase which occur while labeled nucleotides or nucleotide analogs are added to a growing nucleic acid chain which is complementary to the template nucleic acid. The signal indicative of the conformational state of the enzyme is used to determine with higher confidence when true nucleotide or nucleotide analog incorporation events occur, allowing for the improved quality of base calls and sequence determination.

    摘要翻译： 提供了用于模板核酸的单分子测序的系统和方法，其中来自连接到聚合酶的标记的信号用于监测聚合酶中的构象变化，同时将标记的核苷酸或核苷酸类似物加入生长的核酸 与模板核酸互补的链。 当发生真核苷酸或核苷酸类似物并入事件时，使用指示酶构象状态的信号来确定更高的置信度，从而提高碱基调用质量和序列测定。

    Generation of modified polymerases for improved accuracy in single molecule sequencing
    7.
    发明授权
    Generation of modified polymerases for improved accuracy in single molecule sequencing 有权
    修饰聚合酶的生成，提高了单分子测序的准确性

    公开(公告)号：US08420366B2

    公开(公告)日：2013-04-16

    申请号：US12924701

    申请日：2010-09-30

    申请人： Sonya Clark ,  Arek Bibillo ,  Paul Peluso ,  Fred Christians ,  Molly He ,  Insil Park ,  Harold Lee ,  Keith Bjornson ,  Lei Jia ,  Robin Emig

    发明人： Sonya Clark ,  Arek Bibillo ,  Paul Peluso ,  Fred Christians ,  Molly He ,  Insil Park ,  Harold Lee ,  Keith Bjornson ,  Lei Jia ,  Robin Emig

    IPC分类号： C12N9/12 ,  C12P19/34

    CPC分类号： C12N9/12 ,  C12N11/00 ,  C12P19/34 ,  C12Q1/68

    摘要： Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog, are also provided. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

    摘要翻译： 提供了包含重组DNA聚合酶的组合物，其包括氨基酸取代，插入，缺失和/或异源或外源特征，其在聚合酶上赋予改进的性质以用于增强的单分子测序。 这种性质可以包括聚合酶动力学循环的一个或多个步骤的降低的反应速率，增加的闭合聚合酶/ DNA复合物稳定性，增强的金属离子配位，减少的核酸外切酶活性，降低的支化部分等。 还提供了表现出低于其衍生的聚合酶的支化级分的支化级分的聚合酶，或者对于磷酸酯标记的核苷酸类似物的支化级分小于约25％。 还提供了编码具有上述表型的聚合酶的核酸，以及使用这种聚合酶制备DNA或顺序DNA模板的方法。

    SINGLE MOLECULE SEQUENCING WITH TWO DISTINCT CHEMISTRY STEPS
    9.
    发明申请
    SINGLE MOLECULE SEQUENCING WITH TWO DISTINCT CHEMISTRY STEPS 有权
    单分子序列与两个不同的化学步骤

    公开(公告)号：US20110212437A1

    公开(公告)日：2011-09-01

    申请号：US13022926

    申请日：2011-02-08

    申请人： Robin Emig ,  Lei Jia ,  Jeremiah Hanes ,  Lubomir Sebo

    发明人： Robin Emig ,  Lei Jia ,  Jeremiah Hanes ,  Lubomir Sebo

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C07H19/04

    CPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H19/11 ,  C07H19/20 ,  C07H19/213 ,  C12Q1/6874 ,  C12Q2521/301 ,  C12Q2525/186 ,  C12Q2563/107 ,  C12Q2537/157 ,  C12Q2521/319 ,  C12Q2521/525

    摘要： Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3′ hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3′ hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3′ hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids.

    摘要翻译： 提供了用于核酸测序的方法，组合物和系统，其中连续掺入核苷酸使用两个不同的化学步骤。 在选择性切割活性的存在下，可以将多个在其3'羟基上具有标记的离去基团的核苷酸类似物依次加入到生长链中，该选择性裂解活性优选在其引入后优先切割3'羟基离去基团。 选择性切割剂可以包含核酸外切酶活性，并且核酸外切酶活性可以是聚合酶相关的外切核酸酶活性。 在可切割聚磷酸酯部分和3'羟基离去基团上具有标记的核苷酸类似物可以提供核苷酸类似物掺入特征的信号。 具有照明光学器件，收集光学元件和底物的系统在标记被并入生长的核酸链中观察信号，允许对模板核酸进行测序。