公开(公告)号：US06420149B1

公开(公告)日：2002-07-16

申请号：US09182450

申请日：1998-10-30

申请人： Minoru Fukuda ,  Katsutoshi Sasaki ,  Kazumi Miura ,  Satoshi Nakagawa ,  Tatsunari Nishi ,  Susumu Sekine

发明人： Minoru Fukuda ,  Katsutoshi Sasaki ,  Kazumi Miura ,  Satoshi Nakagawa ,  Tatsunari Nishi ,  Susumu Sekine

IPC分类号： C12N900

CPC分类号： C12N9/1051

摘要： The present invention provides pharmaceutical preparations for anti-inflammation, anti-infection, inhibition of cancer metastasis etc., foods such as dairy products etc., and a method for improving proteins, as well as a method for diagnosis of inflammatory diseases and cancer malignancy. According to the present invention, there can be provided a polypeptide having poly-N-acetyllactosamine sugar chains synthesis-related activity, a process for producing the polypeptide, DNA coding for the polypeptide, a process for producing the DNA, a recombinant vector having the DNA integrated therein, a transformant carrying the recombinant vector, an antibody recognizing the polypeptide, a process for producing poly-N-acetyllactosamine sugar chains by use of the DNA or the polypeptide, diagnosis and treatment of diseases such as inflammations, cancers etc. by use of the DNA, the polypeptide or the antibody, determination and immunostaining of the polypeptide of the present invention by use of the antibody, a method for screening a compound varying the expression of a gene coding for the polypeptide, and a method for screening a substance varying the activity of the polypeptide.

摘要翻译： 本发明提供抗炎，抗感染，癌转移抑制等药物制剂，乳制品等食品，以及改善蛋白质的方法，以及炎性疾病和癌症恶性肿瘤的诊断方法 。 根据本发明，可以提供具有聚-N-乙酰乳糖胺糖链合成相关活性的多肽，制备多肽的方法，编码多肽的DNA，制备DNA的方法，具有 整合在其中的DNA，携带重组载体的转化体，识别多肽的抗体，使用DNA或多肽产生聚-N-乙酰乳糖胺糖链的方法，诊断和治疗诸如炎症，癌症等疾病，通过 使用DNA，多肽或抗体，通过使用抗体测定和免疫染色本发明的多肽，筛选改变编码多肽的基因的表达的化合物的方法，以及筛选方法 物质改变多肽的活性。

公开(公告)号：US07291491B2

公开(公告)日：2007-11-06

申请号：US10166098

申请日：2002-06-11

申请人： Minoru Fukuda ,  Katsutoshi Sasaki ,  Kazumi Miura ,  Satoshi Nakagawa ,  Tatsunari Nishi ,  Susumu Sekine

发明人： Minoru Fukuda ,  Katsutoshi Sasaki ,  Kazumi Miura ,  Satoshi Nakagawa ,  Tatsunari Nishi ,  Susumu Sekine

IPC分类号： C12N9/10 ,  C07H21/04 ,  C12P21/06

CPC分类号： C12N9/1051

摘要： The present invention provides pharmaceutical preparations for anti-inflammation, anti-infection, inhibition of cancer metastasis etc., foods such as dairy products etc., and a method for improving proteins, as well as a method for diagnosis of inflammatory diseases and cancer malignancy. According to the present invention, there can be provided a polypeptide having poly-N-acetyllactosamine sugar chains synthesis-related activity, a process for producing the polypeptide, DNA coding for the polypeptide, a process for producing the DNA, a recombinant vector having the DNA integrated therein, a transformant carrying the recombinant vector, an antibody recognizing the polypeptide, a process for producing poly-N-acetyllactosamine sugar chains by use of the DNA or the polypeptide, diagnosis and treatment of diseases such as inflammations, cancers etc. by use of the DNA, the polypeptide or the antibody, determination and immunostaining of the polypeptide of the present invention by use of the antibody, a method for screening a compound varying the expression of a gene coding for the polypeptide, and a method for screening a substance varying the activity of the polypeptide.

摘要翻译： 本发明提供抗炎，抗感染，癌转移抑制等药物制剂，乳制品等食品，以及改善蛋白质的方法，以及炎性疾病和癌症恶性肿瘤的诊断方法 。 根据本发明，可以提供具有聚-N-乙酰乳糖胺糖链合成相关活性的多肽，制备多肽的方法，编码多肽的DNA，制备DNA的方法，具有 整合在其中的DNA，携带重组载体的转化体，识别多肽的抗体，使用DNA或多肽产生聚-N-乙酰乳糖胺糖链的方法，诊断和治疗诸如炎症，癌症等疾病，通过 使用DNA，多肽或抗体，通过使用抗体测定和免疫染色本发明的多肽，筛选改变编码多肽的基因的表达的化合物的方法，以及筛选方法 物质改变多肽的活性。

公开(公告)号：US5494790A

公开(公告)日：1996-02-27

申请号：US309985

申请日：1994-09-20

申请人： Katsutoshi Sasaki ,  Etsuyo Watanabe ,  Tatsunari Nishi ,  Susumu Sekine ,  Nobuo Hanai ,  Mamoru Hasegawa

发明人： Katsutoshi Sasaki ,  Etsuyo Watanabe ,  Tatsunari Nishi ,  Susumu Sekine ,  Nobuo Hanai ,  Mamoru Hasegawa

IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12Q1/68

CPC分类号： C12N9/1081

摘要： There are provided a novel .alpha.2.fwdarw.3 sialyltransferase expressed by a cloned gene from animal cells, a cDNA encoding the .alpha.2.fwdarw.3 sialyltransferase, a method for detecting or suppressing the expression of an .alpha.2.fwdarw.3 sialyltransferase by use of said cDNA, a recombinant vector containing said cDNA, a cell containing said vector, and their production processes.

摘要翻译： 提供了由动物细胞克隆的基因表达的新型α2-> 3唾液酸转移酶，编码α2-> 3唾液酸转移酶的cDNA，通过使用α2-> 3唾液酸转移酶检测或抑制α2-> 3唾液酸转移酶的表达的方法 所述cDNA是含有所述cDNA的重组载体，含有所述载体的细胞及其制备方法。

公开(公告)号：US5384249A

公开(公告)日：1995-01-24

申请号：US991587

申请日：1992-12-16

申请人： Katsutoshi Sasaki ,  Etsuyo Watanabe ,  Tatsunari Nishi ,  Susumu Sekine ,  Nobuo Hanai ,  Mamoru Hasegawa

发明人： Katsutoshi Sasaki ,  Etsuyo Watanabe ,  Tatsunari Nishi ,  Susumu Sekine ,  Nobuo Hanai ,  Mamoru Hasegawa

IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12P21/00

CPC分类号： C12N9/1081

摘要： There are provided a novel .alpha.2.fwdarw.3 sialyltransferase expressed by a cloned gene from animal cells, a cDNA encoding the .alpha.2.fwdarw.3 sialyltransferase, a method for detecting or suppressing the expression of an .alpha.2.fwdarw.3 sialyltransferase by use of said cDNA, a recombinant vector containing said cDNA, a cell containing said vector, and their production processes.

摘要翻译： 提供了由动物细胞克隆的基因表达的新型α2-> 3唾液酸转移酶，编码α2-> 3唾液酸转移酶的cDNA，通过使用α2-> 3唾液酸转移酶检测或抑制α2-> 3唾液酸转移酶的表达的方法 所述cDNA是含有所述cDNA的重组载体，含有所述载体的细胞及其制备方法。

公开(公告)号：US08722366B2

公开(公告)日：2014-05-13

申请号：US13164814

申请日：2011-06-21

申请人： Katsutoshi Sasaki ,  Norihiko Shiraishi ,  Ayumi Natsume ,  Yoji Yamada ,  Satoshi Nakagawa ,  Susumu Sekine

发明人： Katsutoshi Sasaki ,  Norihiko Shiraishi ,  Ayumi Natsume ,  Yoji Yamada ,  Satoshi Nakagawa ,  Susumu Sekine

IPC分类号： C12P19/26 ,  C12P19/18

CPC分类号： C12N9/1051 ,  A61K38/00 ,  C07K2319/02 ,  C12N9/1085

摘要： The present invention provides a novel polypeptide having a β1,3-N-acetylglucosaminyltransferase activity; a method for producing the polypeptide; a DNA which encodes the polypeptide; a recombinant vector into which the DNA is inserted; a transformant comprising the recombinant vector; a method for producing a sugar chain or complex carbohydrate, using the polypeptide; a method for producing a sugar chain or complex carbohydrate, using the transformant; an antibody which recognizes the polypeptide; a method for screening a substance which changes the expression of the gene which encodes the polypeptide; and a method for screening a substance which changes the activity of the polypeptide.

摘要翻译： 本发明提供了具有“1,3-N-乙酰葡糖胺基转移酶”活性的新型多肽; 一种生产该多肽的方法; 编码多肽的DNA; 插入DNA的重组载体; 包含重组载体的转化体; 使用该多肽制备糖链或复合碳水化合物的方法; 使用该转化体生产糖链或复合碳水化合物的方法; 识别多肽的抗体; 用于筛选改变编码多肽的基因表达的物质的方法; 以及筛选改变多肽活性的物质的方法。

公开(公告)号：US20120156732A1

公开(公告)日：2012-06-21

申请号：US13164814

申请日：2011-06-21

申请人： Katsutoshi Sasaki ,  Norihiko Shiraishi ,  Ayumi Natsume ,  Yoji Yamada ,  Satoshi Nakagawa ,  Susumu Sekine

发明人： Katsutoshi Sasaki ,  Norihiko Shiraishi ,  Ayumi Natsume ,  Yoji Yamada ,  Satoshi Nakagawa ,  Susumu Sekine

IPC分类号： C12P19/18

CPC分类号： C12N9/1051 ,  A61K38/00 ,  C07K2319/02 ,  C12N9/1085

摘要： The present invention provides a novel polypeptide having a β1,3-N-acetylglucosaminyltransferase activity; a method for producing the polypeptide; a DNA which encodes the polypeptide; a recombinant vector into which the DNA is inserted; a transformant comprising the recombinant vector; a method for producing a sugar chain or complex carbohydrate, using the polypeptide; a method for producing a sugar chain or complex carbohydrate, using the transformant; an antibody which recognizes the polypeptide; a method for screening a substance which changes the expression of the gene which encodes the polypeptide; and a method for screening a substance which changes the activity of the polypeptide.

摘要翻译： 本发明提供了具有“1,3-N-乙酰葡糖胺基转移酶”活性的新型多肽; 一种生产该多肽的方法; 编码多肽的DNA; 插入DNA的重组载体; 包含重组载体的转化体; 使用该多肽制备糖链或复合碳水化合物的方法; 使用该转化体生产糖链或复合碳水化合物的方法; 识别多肽的抗体; 用于筛选改变编码多肽的基因表达的物质的方法; 以及筛选改变多肽活性的物质的方法。

公开(公告)号：US07629150B2

公开(公告)日：2009-12-08

申请号：US11837804

申请日：2007-08-13

申请人： Hisashi Narimatsu ,  Katsutoshi Sasaki ,  Ayumi Natsume ,  Hiroyuki Mio ,  Satoshi Nakagawa ,  Susumu Sekine ,  Akira Togayachi

发明人： Hisashi Narimatsu ,  Katsutoshi Sasaki ,  Ayumi Natsume ,  Hiroyuki Mio ,  Satoshi Nakagawa ,  Susumu Sekine ,  Akira Togayachi

IPC分类号： C12P19/00 ,  C12P1/00 ,  C12P19/18 ,  C12N9/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07K14/00 ,  C07H21/04

CPC分类号： C12N9/1051 ,  A01K2217/05 ,  A01K2217/075 ,  C07K2319/00 ,  C12N9/1241 ,  C12N2799/026 ,  C12Y204/0108

摘要： The present invention provides a novel polypeptide having a β1,3-N-acetylglucosaminyltransferase activity, an agent for synthesizing a sugar chain comprising the polypeptide, a process for producing a sugar chain or a complex carbohydrate using the agent for synthesizing a sugar chain, DNA encoding the polypeptide, a process for producing the polypeptide, an antibody against the polypeptide, and a diagnosis method and a medicament for treatment for inflammation, cancer or tumor metastasis using the DNA or the antibody. The present invention is useful for synthesis of a useful sugar chain and diagnosis and treatment for inflammatory diseases, cancer or tumor metastasis.

摘要翻译： 本发明提供了具有β1,3-N-乙酰葡糖胺基转移酶活性的新型多肽，用于合成包含该多肽的糖链的试剂，使用该合成糖链的试剂生产糖链或复合碳水化合物的方法 编码多肽，制备多肽的方法，针对多肽的抗体，以及诊断方法和用于使用DNA或抗体治疗炎症，癌症或肿瘤转移的药物。 本发明可用于合成有用的糖链，并用于炎性疾病，癌症或肿瘤转移的诊断和治疗。

公开(公告)号：US20090068720A1

公开(公告)日：2009-03-12

申请号：US12019793

申请日：2008-01-25

申请人： Katsutoshi Sasaki ,  Norihiko Shiraishi ,  Ayumi Natsume ,  Yoji Yamada ,  Satoshi Nakagawa ,  Susumu Sekine

发明人： Katsutoshi Sasaki ,  Norihiko Shiraishi ,  Ayumi Natsume ,  Yoji Yamada ,  Satoshi Nakagawa ,  Susumu Sekine

IPC分类号： C12N9/10

CPC分类号： C12N9/1051 ,  A61K38/00 ,  C07K2319/02 ,  C12N9/1085

摘要： The present invention provides a novel polypeptide having a β1,3-N-acetylglucosaminyl transferase activity; a method for producing the polypeptide; a DNA which encodes the polypeptide; a recombinant vector into which the DNA is inserted; a transformant comprising the recombinant vector; a method for producing a sugar chain or complex carbohydrate, using the polypeptide; a method for producing a sugar chain or complex carbohydrate, using the transformant; an antibody which recognizes the polypeptide; a method for screening a substance which changes the expression of the gene which encodes the polypeptide; and a method for screening a substance which changes the activity of the polypeptide.

摘要翻译： 本发明提供了具有β1,3-N-乙酰氨基葡糖酰转移酶活性的新型多肽; 一种生产该多肽的方法; 编码多肽的DNA; 插入DNA的重组载体; 包含重组载体的转化体; 使用该多肽制备糖链或复合碳水化合物的方法; 使用该转化体制备糖链或复合碳水化合物的方法; 识别多肽的抗体; 用于筛选改变编码多肽的基因表达的物质的方法; 以及筛选改变多肽活性的物质的方法。

公开(公告)号：US20080050745A1

公开(公告)日：2008-02-28

申请号：US11837804

申请日：2007-08-13

申请人： Hisashi Narimatsu ,  Katsutoshi Sasaki ,  Ayumi Natsume ,  Hiroyuki Mio ,  Satoshi Nakagawa ,  Susumu Sekine ,  Akira Togayachi

发明人： Hisashi Narimatsu ,  Katsutoshi Sasaki ,  Ayumi Natsume ,  Hiroyuki Mio ,  Satoshi Nakagawa ,  Susumu Sekine ,  Akira Togayachi

IPC分类号： C12Q1/68 ,  A01K67/00 ,  C07H21/04 ,  C12N1/00 ,  C12N15/54 ,  C12N5/10 ,  G01N33/53 ,  C12N9/10 ,  C12N9/99 ,  C12P19/04 ,  C12P21/00

CPC分类号： C12N9/1051 ,  A01K2217/05 ,  A01K2217/075 ,  C07K2319/00 ,  C12N9/1241 ,  C12N2799/026 ,  C12Y204/0108

摘要： The present invention provides a novel polypeptide having a β1,3-N-acetylglucosaminyltransferase activity, an agent for synthesizing a sugar chain comprising the polypeptide, a process for producing a sugar chain or a complex carbohydrate using the agent for synthesizing a sugar chain, DNA encoding the polypeptide, a process for producing the polypeptide, an antibody against the polypeptide, and a diagnosis method and a medicament for treatment for inflammation, cancer or tumor metastasis using the DNA or the antibody. The present invention is useful for synthesis of a useful sugar chain and diagnosis and treatment for inflammatory diseases, cancer or tumor metastasis.

摘要翻译： 本发明提供了具有β1,3-N-乙酰葡糖胺基转移酶活性的新型多肽，用于合成包含该多肽的糖链的试剂，使用该合成糖链的试剂生产糖链或复合碳水化合物的方法 编码多肽，制备多肽的方法，针对多肽的抗体，以及诊断方法和用于使用DNA或抗体治疗炎症，癌症或肿瘤转移的药物。 本发明可用于合成有用的糖链，并用于炎性疾病，癌症或肿瘤转移的诊断和治疗。

公开(公告)号：US07279310B2

公开(公告)日：2007-10-09

申请号：US10363145

申请日：2001-08-31

申请人： Hisashi Narimatsu ,  Katsutoshi Sasaki ,  Ayumi Natsume ,  Hiroyuki Mio ,  Satoshi Nakagawa ,  Susumu Sekine ,  Akira Togayachi

发明人： Hisashi Narimatsu ,  Katsutoshi Sasaki ,  Ayumi Natsume ,  Hiroyuki Mio ,  Satoshi Nakagawa ,  Susumu Sekine ,  Akira Togayachi

IPC分类号： C12P19/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/1051 ,  A01K2217/05 ,  A01K2217/075 ,  C07K2319/00 ,  C12N9/1241 ,  C12N2799/026 ,  C12Y204/0108

摘要： The present invention provides a novel polypeptide having a β1,3-N-acetylglucosaminyltransferase activity, an agent for synthesizing a sugar chain comprising the polypeptide, a process for producing a sugar chain or a complex carbohydrate using the agent for synthesizing a sugar chain, DNA encoding the polypeptide, a process for producing the polypeptide, an antibody against the polypeptide, and a diagnosis method and a medicament for treatment for inflammation, cancer or tumor metastasis using the DNA or the antibody. The present invention is useful for synthesis of a useful sugar chain and diagnosis and treatment for inflammatory diseases, cancer or tumor metastasis.

摘要翻译： 本发明提供了具有β1,3-N-乙酰葡糖胺基转移酶活性的新型多肽，用于合成包含该多肽的糖链的试剂，使用该合成糖链的试剂生产糖链或复合碳水化合物的方法 编码多肽，制备多肽的方法，针对多肽的抗体，以及诊断方法和用于使用DNA或抗体治疗炎症，癌症或肿瘤转移的药物。 本发明可用于合成有用的糖链，并用于炎性疾病，癌症或肿瘤转移的诊断和治疗。