公开(公告)号：US08309914B2

公开(公告)日：2012-11-13

申请号：US12359526

申请日：2009-01-26

申请人： Mircea Guna ,  Yves Le Blanc ,  Bruce Collings

发明人： Mircea Guna ,  Yves Le Blanc ,  Bruce Collings

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/426 ,  H01J49/0063 ,  H01J49/24

摘要： Methods for fragmenting ions in an ion trap are described. These methods involve a) selecting parent ions for fragmentation; b) retaining the parent ions within the ion trap for a retention time interval, the ion trap having an operating pressure of less than about 1×10−4 Torr; c) providing a RF trapping voltage to the ion trap to provide a Mathieu stability parameter q at an excitement level during an excitement time interval within the retention time interval; d) providing a resonant excitation voltage to the ion trap during the excitement time interval to excite and fragment the parent ions; e) providing a non-steady-state pressure increase of at least 10% of the operating pressure within the ion trap by delivering a neutral gas into the ion trap for at least a portion of the retention time interval to raise the pressure in the ion trap to a varying first elevated-pressure in the range between about 6×10−5 Torr to about 5×10−4 Torr for a first elevated-pressure duration; and f) within the retention time interval and after the excitement time interval, terminating the resonant excitation voltage and changing the RF trapping voltage applied to the ion trap to reduce the Mathieu stability parameter q to a hold level less than the excitement level to retain fragments of the parent ions within the ion trap. The excitation time interval and the first elevated-pressure duration substantially overlap in time.

摘要翻译： 描述了在离子阱中分离离子的方法。 这些方法包括：a）选择亲本离子进行碎裂; b）将母离子保留在离子阱内保持时间间隔，离子阱具有小于约1×10-4乇的工作压力; c）向离子阱提供RF捕获电压，以在保持时间间隔内的兴奋时间间隔期间以兴奋水平提供Mathieu稳定性参数q; d）在兴奋时间间隔期间向离子阱提供共振激发电压以激发和分裂母体离子; e）通过将中性气体输送到离子阱中至少保留时间间隔的一部分来提供至少10％的离子阱内的工作压力的非稳态压力增加，以提高离子中的压力 在第一升高压力持续时间内，在约6×10 -5 Torr至约5×10 -4 Torr之间的范围内捕获变化的第一升高压力; 和f）在保持时间间隔内和在兴奋时间间隔之后，终止谐振激励电压并改变施加到离子阱的RF捕获电压，以将Mathieu稳定性参数q降低到小于兴奋水平的保持水平以保留碎片 的离子阱内的母离子。 激发时间间隔和第一升高压力时间基本上在时间上重叠。

公开(公告)号：US20130181125A1

公开(公告)日：2013-07-18

申请号：US13817189

申请日：2011-08-18

申请人： Mircea Guna ,  Bruce Collings

发明人： Mircea Guna ,  Bruce Collings

IPC分类号： H01J49/00 ,  H01J49/06

CPC分类号： H01J49/0031 ,  H01J49/025 ,  H01J49/06 ,  H01J49/061

摘要： A mass spectrometer system can include a mass analyzer operable to mass transmit streams of ions to a detector in a mass dependent fashion for measurement of ion flux intensity. An ion attenuator can be located in the extraction region between the mass analyzer and detector, downstream of the mass analyzer, and can be operable to provide selective attenuation of the ion beam by attenuating ion flux intensity also in mass dependent fashion. Higher concentration ions can be selected and attenuated, while other lower concentration ions can be left unattenuated. Different ions can be attenuated to different degrees. Locating the ion attenuator downstream of the mass analyzer so that the ion beam is already mass differentiated when attenuated can avoid mass discriminatory effects associated with ion beam attenuators. Selective attenuation of only certain ions but not others can extend the dynamic range of the detector without necessarily sacrificing detector sensitivity.

摘要翻译： 质谱仪系统可以包括质量分析器，其可以质量传递离子流到检测器以质量依赖的方式来测量离子通量强度。 离子衰减器可以位于质量分析器和检测器之间的质量分析器下游的提取区域中，并且可以通过以质量依赖的方式衰减离子通量强度来操作来提供离子束的选择性衰减。 可以选择和减弱更高浓度的离子，而其他低浓度离子可以保持不衰减。 不同的离子可以衰减到不同的程度。 将离子衰减器定位在质量分析仪的下游，以便当衰减时离子束已经被质量分化，可以避免与离子束衰减器相关的质量差异影响。 只有某些离子而不是其他离子的选择性衰减可以扩展检测器的动态范围，而不必牺牲检测器灵敏度。

公开(公告)号：US20060169891A1

公开(公告)日：2006-08-03

申请号：US11315788

申请日：2005-12-22

申请人： Bruce Collings ,  Thomas Covey ,  Mircea Guna ,  Hassan Javaheri ,  Alexandre Loboda ,  Bradley Schneider ,  Bruce Thomson

发明人： Bruce Collings ,  Thomas Covey ,  Mircea Guna ,  Hassan Javaheri ,  Alexandre Loboda ,  Bradley Schneider ,  Bruce Thomson

IPC分类号： B01D59/44

CPC分类号： H01J49/067 ,  H01J49/063

摘要： In a mass spectrometer, ions from an ion source pass through an inlet aperture into a vacuum chamber for transmitting prior to mass analysis by the mass analyzer. The configuration of the inlet aperture forms a sonic orifice or sonic nozzle and with a predetermined vacuum chamber pressure, a supersonic free jet expansion is created in the vacuum chamber that entrains the ions within the barrel shock and Mach disc. Once formed, at least one ion guide with a predetermined cross-section to essentially radially confine the supersonic free jet expansion can focus the ions for transmission through the vacuum chamber. This effectively improves the ion transmission between the ion source and the mass analyzer.

摘要翻译： 在质谱仪中，质子分析仪进行质量分析之前，离子源的离子通过入口孔进入真空室以进行传输。 入口孔的构型形成声孔或声波喷嘴，并且在预定的真空室压力下，在真空室中产生超音速自由射流膨胀，该真空室将离子夹带在筒形冲击和马赫盘内。 一旦形成，具有预定横截面以至基本上径向限制超音速自由射流膨胀的至少一个离子导向器可以将离子聚焦以透过真空室。 这有效地改善离子源和质量分析器之间的离子传递。

公开(公告)号：US20060151692A1

公开(公告)日：2006-07-13

申请号：US11032376

申请日：2005-01-10

申请人： Bruce Collings ,  Mircea Guna ,  Hassan Javaheri ,  Alexandre Loboda ,  Bruce Thomson

发明人： Bruce Collings ,  Mircea Guna ,  Hassan Javaheri ,  Alexandre Loboda ,  Bruce Thomson

IPC分类号： B01D59/44

CPC分类号： H01J49/04 ,  H01J49/062

摘要： In a mass spectrometer, ions from an ion source pass through an inlet aperture into a vacuum chamber for transmitting prior to mass analysis by the mass analyzer. The configuration of the inlet aperture forms a sonic orifice or sonic nozzle and with a predetermined vacuum chamber pressure, a supersonic free jet expansion is created in the vacuum chamber that entrains the ions within the barrel shock and Mach disc. Once formed, an ion guide with a predetermined cross-section to essentially radially confine the supersonic free jet expansion can focus the ions for transmission through the vacuum chamber. This effectively improves the ion transmission between the ion source and the mass analyzer.

摘要翻译： 在质谱仪中，质子分析仪进行质量分析之前，离子源的离子通过入口孔进入真空室以进行传输。 入口孔的构型形成声孔或声波喷嘴，并且在预定的真空室压力下，在真空室中产生超音速自由射流膨胀，该真空室将离子夹带在筒形冲击和马赫盘内。 一旦形成，具有预定横截面以基本上径向限制超音速自由射流膨胀的离子导向器可将离子聚焦以透过真空室。 这有效地改善离子源和质量分析器之间的离子传递。

公开(公告)号：US08237109B2

公开(公告)日：2012-08-07

申请号：US12359621

申请日：2009-01-26

申请人： Mircea Guna ,  Bruce Collings

发明人： Mircea Guna ,  Bruce Collings

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/005 ,  H01J49/063 ,  H01J49/4225

摘要： Methods for fragmenting ions retained in an ion trap are described. In various embodiments, a non-steady-state pressure of a neutral collision gas of less than about 5×10−4 Torr and an excitation amplitude of less than about 500 mV (peak to ground) is used to fragment ions with greater than about 80% fragmentation efficiency. In various embodiments, duration of ion excitation is greater than about 25 ms.

摘要翻译： 描述了保留在离子阱中的分离离子的方法。 在各种实施方案中，使用小于约5×10-4乇的中性碰撞气体的非稳定状态压力和小于约500mV（峰对地）的激发振幅来分离大于约 80％的碎片效率。 在各种实施例中，离子激发的持续时间大于约25ms。

公开(公告)号：US20090194686A1

公开(公告)日：2009-08-06

申请号：US12359621

申请日：2009-01-26

申请人： Mircea Guna ,  Bruce Collings

发明人： Mircea Guna ,  Bruce Collings

IPC分类号： B01D59/44

CPC分类号： H01J49/005 ,  H01J49/063 ,  H01J49/4225

摘要： Methods for fragmenting ions retained in an ion trap are described. In various embodiments, a non-steady-state pressure of a neutral collision gas of less than about 5×10−4 Torr and an excitation amplitude of less than about 500 mV (peak to ground) is used to fragment ions with greater than about 80% fragmentation efficiency. In various embodiments, duration of ion excitation is greater than about 25 ms.

摘要翻译： 描述了保留在离子阱中的分离离子的方法。 在各种实施方案中，使用小于约5×10-4乇的中性碰撞气体的非稳定状态压力和小于约500mV（峰对地）的激发振幅用于将大于约80％ 碎片效率。 在各种实施例中，离子激发的持续时间大于约25ms。

公开(公告)号：US20090194684A1

公开(公告)日：2009-08-06

申请号：US12359526

申请日：2009-01-26

申请人： Mircea Guna ,  Yves Le Blanc ,  Bruce Collings

发明人： Mircea Guna ,  Yves Le Blanc ,  Bruce Collings

IPC分类号： B01D59/44

CPC分类号： H01J49/426 ,  H01J49/0063 ,  H01J49/24

摘要： Methods for fragmenting ions in an ion trap are described. These methods involve a) selecting parent ions for fragmentation; b) retaining the parent ions within the ion trap for a retention time interval, the ion trap having an operating pressure of less than about 1×10-4 Torr; c) providing a RF trapping voltage to the ion trap to provide a Mathieu stability parameter q at an excitement level during an excitement time interval within the retention time interval; d) providing a resonant excitation voltage to the ion trap during the excitement time interval to excite and fragment the parent ions; e) providing a non-steady-state pressure increase of at least 10% of the operating pressure within the ion trap by delivering a neutral gas into the ion trap for at least a portion of the retention time interval to raise the pressure in the ion trap to a varying first elevated-pressure in the range between about 6×10-5 Torr to about 5×10-4 Torr for a first elevated-pressure duration; and f within the retention time interval and after the excitement time interval, terminating the resonant excitation voltage and changing the RF trapping voltage applied to the ion trap to reduce the Mathieu stability parameter q to a hold level less than the excitement level to retain fragments of the parent ions within the ion trap. The excitation time interval and the first elevated-pressure duration substantially overlap in time.

摘要翻译： 描述了在离子阱中分离离子的方法。 这些方法包括：a）选择亲本离子进行碎裂; b）将母离子保留在离子阱内保持时间间隔，离子阱具有小于约1×10-4乇的操作压力; c）向离子阱提供RF捕获电压，以在保持时间间隔内的兴奋时间间隔期间以兴奋水平提供Mathieu稳定性参数q; d）在兴奋时间间隔期间向离子阱提供共振激发电压以激发和分裂母体离子; e）通过将中性气体输送到离子阱中至少保留时间间隔的一部分来提供至少10％的离子阱内的工作压力的非稳态压力增加，以提高离子中的压力 在第一升高的压力持续时间内在约6×10 -5 Torr至约5×10 -4 Torr之间的范围内捕获到变化的第一升高压力; 和f在保持时间间隔内和在兴奋时间间隔之后，终止谐振激励电压并改变施加到离子阱的RF捕获电压，以将Mathieu稳定性参数q降低到小于兴奋水平的保持水平以保留 离子阱内的母离子。 激发时间间隔和第一升高压力时间基本上在时间上重叠。

公开(公告)号：US06703607B2

公开(公告)日：2004-03-09

申请号：US10159766

申请日：2002-05-30

申请人： William R. Stott ,  Bruce Collings ,  Frank Londry ,  James Hager

发明人： William R. Stott ,  Bruce Collings ,  Frank Londry ,  James Hager

IPC分类号： H01J4942

CPC分类号： H01J49/429 ,  H01J49/4225

摘要： An improved method of operating a mass spectrometer having a linear ion trap wherein ions are axially ejected from the trap to a detector or subsequent mass analysis stage. The DC barrier field produced at the exit lens of the trap is scanned in conjunction with the scanning of other fields used to energize ions of select mass-to-charge ratios past the barrier field/exit lens. The technique can maximize the resolution obtainable from axial ejection over a wide mass range.

摘要翻译： 一种操作具有线性离子阱的质谱仪的改进方法，其中离子从捕集器轴向地从捕获器喷射到检测器或随后的质量分析阶段。 结合用于激励通过屏障场/出射透镜的选择质荷比的离子的其它场的扫描来扫描在阱的出射透镜处产生的DC势垒场。 该技术可以使在大质量范围内从轴向喷射获得的分辨率最大化。

公开(公告)号：US08110798B2

公开(公告)日：2012-02-07

申请号：US12359563

申请日：2009-01-26

申请人： Bruce Collings

发明人： Bruce Collings

IPC分类号： H01J49/04

CPC分类号： H01J49/0481 ,  H01J49/422

摘要： Methods for cooling ions retained in an ion trap are described. In various embodiments, a cooling gas is delivered into a linear ion trap causing a non-steady state pressure elevation in at least a portion of the trap above about 8×10−5 Torr for a duration less than the ion-retention time. In various embodiments, the duration of pressure elevation can be based upon a period of time required for an ion to lose a desired amount of its kinetic energy.

摘要翻译： 描述了保留在离子阱中的离子的冷却方法。 在各种实施方案中，将冷却气体输送到线性离子阱中，导致捕集阱的至少一部分中的非稳态压力升高高于约8×10 -5 Torr持续时间小于离子保留时间。 在各种实施方案中，压力升高的持续时间可以基于离子失去所需量的其动能所需的时间段。

公开(公告)号：US20100237236A1

公开(公告)日：2010-09-23

申请号：US12408026

申请日：2009-03-20

申请人： Bruce Thomson ,  Bruce Collings

发明人： Bruce Thomson ,  Bruce Collings

IPC分类号： B01D59/44

CPC分类号： H01J49/0045 ,  H01J49/4225 ,  H01J49/427

摘要： A method of processing multiple precursor ions in a tandem mass spectrometer includes generating a plurality of precursor ions with an ion source. At least some of the plurality of precursor ions is trapped in an ion trap. At least two precursor ions of interest are isolated from the plurality of precursor ions with a filtered noise field. Precursor ions of interest are sequentially ejected toward a collision cell. The sequentially ejected precursor ions of interest are fragmented in a collision cell. The mass-to-charge ratio spectra of the fragmented ions are then determined with a mass spectrometer.

摘要翻译： 在串联质谱仪中处理多种前体离子的方法包括用离子源生成多个前体离子。 多个前体离子中的至少一些被捕获在离子阱中。 感兴趣的至少两个前体离子与多个前体离子与已滤波的噪声场分离。 感兴趣的前体离子顺序地朝向碰撞池排出。 感兴趣的顺序排出的前体离子在碰撞池中碎裂。 然后用质谱仪测定碎片离子的质荷比谱。