公开(公告)号：US5013665A

公开(公告)日：1991-05-07

申请号：US273367

申请日：1988-11-17

申请人： Motoshi Suzuki ,  Howard Dalton ,  Anthony O. Richards ,  Stephen H. Stanley

发明人： Motoshi Suzuki ,  Howard Dalton ,  Anthony O. Richards ,  Stephen H. Stanley

IPC分类号： C12N1/38

CPC分类号： C12N1/38 ,  Y10S435/858 ,  Y10S435/859 ,  Y10S435/863

摘要： The present invention relates a method for reactivating or regenerating the methane oxidizability of a methane-utilizing bacteria which have partly or wholly lost its methane oxidizability by culturing said methane-utilizing bacteria in a specified condition, and further relates to a method for continuous production of oxides by bringing a methane-utilizing bacteria in contact with alkanes, alkenes or cyclic compounds.

摘要翻译： 本发明涉及一种通过在特定条件下培养所述甲烷利用细菌而部分或完全丧失其甲烷氧化性的甲烷利用细菌的甲烷可氧化性的再生或再生方法，还涉及连续生产 通过使甲烷利用细菌与烷烃，烯烃或环状化合物接触来进行氧化。

公开(公告)号：US20090004656A1

公开(公告)日：2009-01-01

申请号：US11962067

申请日：2007-12-20

申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

IPC分类号： C12Q1/68 ,  C12Q1/48 ,  C12N9/10 ,  C12N15/11 ,  C12P19/34

CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。

公开(公告)号：US07020664B1

公开(公告)日：2006-03-28

申请号：US09723972

申请日：2000-11-28

申请人： Tomoko Yamaguchi ,  Toshio Mizuno ,  Motoshi Suzuki

发明人： Tomoko Yamaguchi ,  Toshio Mizuno ,  Motoshi Suzuki

IPC分类号： G06F12/00

CPC分类号： G06F17/30067

摘要： Upon receipt of a segment name obtainment request, the segment name generation unit 104 generates a segment name composed of (1) a file name of a file to which the segment belongs and (2) a number indicating a serial number of the segment in the file. Upon receipt of an access request specifying a segment name, the segment identification unit 119 refers to the segment name to detect the file name and the serial number of the segment in the detected file. The segment position detection unit 106 then refers to the file information storage unit 102 and the segment information storage unit 103 to detect a position where a segment is to be accessed.

摘要翻译： 在接收到段名称获取请求时，段名称生成单元104生成由（1）段所属的文件的文件名构成的段名，（2）表示段的名称的序号， 文件。 在接收到指定段名称的访问请求时，段识别单元119参考段名来检测检测文件中的段的文件名和序列号。 然后，段位置检测单元106参考文件信息存储单元102和段信息存储单元103以检测段被访问的位置。

公开(公告)号：US06398316B1

公开(公告)日：2002-06-04

申请号：US09285052

申请日：1999-04-02

申请人： Yasuji Mizutani ,  Motoshi Suzuki ,  Hirofumi Nitta

发明人： Yasuji Mizutani ,  Motoshi Suzuki ,  Hirofumi Nitta

IPC分类号： B60T844

CPC分类号： F02D9/02 ,  B60T8/4809 ,  B60T13/683 ,  B60T13/686 ,  B60T13/72

摘要： A vacuum booster apparatus used for a brake apparatus positively decreases a degree of opening of a throttle valve when it is required while an unnecessary decrease in the degree of opening of the throttle valve is positively prevented. A first pressure chamber of a vacuum booster is connected to an intake pipe of the engine on a downstream side of a throttle valve. A second pressure chamber of the vacuum booster is selectively connectable to one of the first pressure chamber and atmosphere. The vacuum booster boosts an operating force applied to a brake operating member based on a pressure difference between the first pressure chamber and the second pressure chamber. A throttle-opening-degree control device controls a negative pressure in the first pressure chamber by controlling the degree of opening of the throttle valve. The throttle-opening-degree control device decreases the degree of opening of the throttle valve only for a limited time when the negative pressure in the first pressure chamber is smaller than a predetermined necessary negative pressure determined by an operating-force relating amount related to the operating force applied to the brake operating member.

公开(公告)号：US06395524B2

公开(公告)日：2002-05-28

申请号：US08978806

申请日：1997-11-26

申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

IPC分类号： C12N910

CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。

公开(公告)号：US5941128A

公开(公告)日：1999-08-24

申请号：US950438

申请日：1997-10-15

申请人： Taizo Toyama ,  Motoshi Suzuki

发明人： Taizo Toyama ,  Motoshi Suzuki

IPC分类号： B23Q1/00 ,  B23Q1/44 ,  B25J9/04 ,  B25J11/00 ,  B25J17/00 ,  B25J17/02 ,  B25J18/00 ,  F16B7/10

CPC分类号： B25J17/0266 ,  B25J17/0208 ,  Y10T403/32008 ,  Y10T409/308232 ,  Y10T74/20335

摘要： A machine tool includes a base, a traveling plate to which a tool is to be attached, plural rods, and plural drive mechanisms fixed to the base. Each of the drive mechanisms is provided with a slide table movable on the drive mechanism and with an actuator for moving the slide table. One end of each of the rods is swingably connected to the corresponding slide tables through a first joint and the other end of each of the rods is swingably connected to the traveling plate through a second joint. Each of the first and the second joints include two rolling elements, each of which is rotatably supported by a rolling bearing to swing the rod.As the rolling elements are supported by the rolling bearings without a clearance, the positioning accuracy of the machine tool is enhanced.

摘要翻译： 机床包括基座，待安装工具的行进板，多个杆和固定到基座的多个驱动机构。 每个驱动机构设置有可在驱动机构上移动的滑动台和用于移动滑动台的致动器。 每个杆的一端通过第一接头可摆动地连接到相应的滑动台，并且每个杆的另一端通过第二接头可摆动地连接到行进板。 第一和第二关节中的每一个包括两个滚动元件，每个滚动元件由滚动轴承可旋转地支撑以摆动杆。 由于滚动体由滚动轴承支撑而没有间隙，因此机床的定位精度得以提高。

公开(公告)号：US20080255744A1

公开(公告)日：2008-10-16

申请号：US12100217

申请日：2008-04-09

申请人： Yoshiyuki Yasui ,  Hiroaki Aizawa ,  Motoshi Suzuki

发明人： Yoshiyuki Yasui ,  Hiroaki Aizawa ,  Motoshi Suzuki

IPC分类号： G06F19/00

CPC分类号： B60T13/662 ,  B60T8/1755 ,  B60T8/17616 ,  B60T8/1766 ,  B60T13/686

摘要： A brake control apparatus for a vehicle includes four wheel braking apparatuses, a first hydraulic pressure generating apparatus, a front-wheel hydraulic circuit connecting the hydraulic pressure generating apparatus to the two of the wheel braking apparatuses, a rear-wheel hydraulic circuit connecting the hydraulic pressure generating apparatus to the other two of the wheel braking apparatuses, a second hydraulic pressure generating apparatus generating an auxiliary pressure, a braking operation variable detecting means, a reference amount determining means for determining a reference amount of auxiliary hydraulic pressure, an obtaining means for obtaining at least one of state quantities indicating a load condition, a driving condition and slipperiness of wheel, a target amount determining means determining a target amount of auxiliary hydraulic pressure of each hydraulic circuit to be equal to or greater than the corresponding reference amount of auxiliary hydraulic pressure, and a pressure regulating means for regulating the auxiliary hydraulic pressure.

摘要翻译： 车辆的制动控制装置包括四轮制动装置，第一液压发生装置，将液压发生装置连接到两个车轮制动装置的前轮液压回路，连接液压的后轮液压回路 另外两个车轮制动装置的压力发生装置，产生辅助压力的第二液压发生装置，制动操作变量检测装置，用于确定辅助液压参考量的基准量确定装置，用于 获得指示负载状态，车辆的行驶状况和滑动性的状态量中的至少一个，将每个液压回路的辅助液压的目标量确定为相应的辅助参考量的目标量确定装置 液压，和a 用于调节辅助液压的压力调节装置。

公开(公告)号：US07312059B2

公开(公告)日：2007-12-25

申请号：US11096645

申请日：2005-03-31

申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

IPC分类号： C12N9/10 ,  C07K1/00

CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。

公开(公告)号：US07233238B2

公开(公告)日：2007-06-19

申请号：US10940980

申请日：2004-09-15

申请人： Motoshi Suzuki ,  Hidenori Kajino ,  Minao Yanase

发明人： Motoshi Suzuki ,  Hidenori Kajino ,  Minao Yanase

IPC分类号： B60C23/00

CPC分类号： B60C23/061 ,  Y10T137/36

摘要： A tire air-pressure alarming device includes wheel speed sensors for detecting wheel speeds of wheels of a motor vehicle and also includes a microcomputer-base control device and an alarming unit. The control device is programmed to execute function steps of: deriving a decision value based on the wheel speeds detected by the wheel speed sensors; judging the occurrence of air-pressure drop of the tires constituting wheels, based on the decision value; and outputting a command when the occurrence of the air-pressure drop of the tires is judged. The alarming unit is responsive to the command from the control device thereby to raise an alarm indicating the occurrence of the air-pressure drop of the tire. The tires are monitored by the tire air-pressure alarming device, wherein the control device is also programmed to execute a function step of standardizing the speed dependency of a rolling radius of the tires thereby to standardize the performance in judging the air-pressure drop of the tires. Thus, the tire property can be controlled to improve the accuracy in detecting the tire air-pressure drop.

摘要翻译： 轮胎气压报警装置包括用于检测机动车辆车轮速度的车轮速度传感器，还包括微机基座控制装置和报警单元。 控制装置被编程为执行功能步骤：基于由车轮速度传感器检测到的车轮速度导出判定值; 基于所述判定值判断构成车轮的轮胎的气压下降的发生; 并且当判断出轮胎的气压降的发生时输出命令。 报警单元响应于来自控制装置的命令，从而提高指示轮胎气压降的发生的报警。 轮胎由轮胎气压报警装置进行监视，其中控制装置也被编程为执行标准化轮胎的滚动半径的速度依赖性的功能步骤，从而标准化判断空气压降的性能 轮胎。 因此，可以控制轮胎性能，以提高检测轮胎空气压降的精度。

公开(公告)号：US07063393B2

公开(公告)日：2006-06-20

申请号：US10216851

申请日：2002-08-13

申请人： Motoshi Suzuki ,  Haruo Arakawa ,  Takayuki Takeshita ,  Takahisa Yokoyama

发明人： Motoshi Suzuki ,  Haruo Arakawa ,  Takayuki Takeshita ,  Takahisa Yokoyama

IPC分类号： B60T13/74

CPC分类号： B60T8/4077 ,  B60T7/042 ,  B60T8/24 ,  B60T8/326 ,  B60T8/3275 ,  B60T8/4275 ,  B60T8/4845 ,  B60T13/745 ,  B60T2220/04 ,  B60T2270/603

摘要： In an electronic brake system, a brake pedal is operated by a driver according to a requisite braking force. Wheel cylinders equipped at respective vehicle wheels generate braking forces at the respective vehicle wheels. A master cylinder applies a brake fluid pressure to generate the respective wheel cylinder pressures. A pedal operation amount detection portion detects a stroke amount of the brake pedal. The brake pedal and the master cylinder are isolated from each other. The motor is driven based on the pedal operation amount detected by the pedal operation amount detection portion and then controls the brake fluid pressure.