公开(公告)号：US09605029B2

公开(公告)日：2017-03-28

申请号：US14763589

申请日：2013-12-25

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Hideki Watanabe ,  Shinya Honda

IPC: C07K14/00 ,  C07K7/06 ,  C07K7/08 ,  G01N33/68 ,  C07K16/42

CPC classification number: C07K14/001 ,  C07K7/06 ,  C07K7/08 ,  C07K16/4208 ,  G01N33/6854

Abstract: Polypeptides which have binding activity to an Fc region of immunoglobulin G and can be favorably used in detecting, purifying, immobilizing or removing an antibody, immunoglobulin G or a protein containing an Fc region of immunoglobulin G, are described. Methods for detecting, purifying, immobilizing or removing an antibody, immunoglobulin G or a protein containing an Fc region of immunoglobulin G, by using the peptide, are also described.

公开(公告)号：US10941179B2

公开(公告)日：2021-03-09

申请号：US16184028

申请日：2018-11-08

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Yukako Senga ,  Hideki Watanabe ,  Shinya Honda

IPC: C07K1/22 ,  C07K16/00 ,  C07K1/14 ,  C07K1/02 ,  C07K14/00 ,  C07K7/06 ,  C07K7/08 ,  C07K1/32 ,  C07K16/06 ,  C07K1/36 ,  A61K39/395

Abstract: The present invention relates to a method for suppressing aggregation of polypeptide. Specifically, the present invention relates to a method for suppressing, in a solution comprising an antibody or an Fc region-containing protein, formation of an aggregate derived from an antibody or an Fc region-containing protein having a non-native conformation, the method comprising: the steps of (i) binding an AF.2A1 polypeptide or an analog thereof with an aggregate derived from the antibody or Fc region-containing protein having a non-native conformation in the solution; and (ii) collecting the aggregate bound to the polypeptide or analog thereof from the solution.

公开(公告)号：US11008365B2

公开(公告)日：2021-05-18

申请号：US16329812

申请日：2017-08-31

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Hideki Watanabe ,  Shinya Honda

IPC: C07K1/22 ,  C07K7/06 ,  C07K7/08 ,  C07K16/42 ,  C07K16/00 ,  C07K16/06 ,  C12N7/00 ,  C12N15/62

Abstract: The present invention relates to a novel polypeptide having affinity for proteins partially including a CH1-CL domain forming a non-native three-dimensional structure and capable of being suitably used for detecting, immobilizing, or removing these proteins and relates to use of the polypeptide. Specifically, disclosed are a polypeptide consisting of an amino acid sequence represented by any one of the following formulas 1 to 3: (1) P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 1), (2) Y-D-P-E-T-G-T-W-P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 4), and (3) P-N-S-G-G-G-G-S-Y-D-P-E-T-G-T-W-P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 7) (wherein x represents an amino acid residue; and brackets represent any one of the amino acid residues within the brackets), and a method of using the polypeptide to detect, purify, or remove a protein partially including a CH1-CL domain forming a non-native three-dimensional structure.

公开(公告)号：US09897611B2

公开(公告)日：2018-02-20

申请号：US14655792

申请日：2013-12-09

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Shinya Honda ,  Hideki Watanabe ,  Kazuhiko Yamasaki

IPC: C12N7/06 ,  G01N33/68 ,  C12N15/10 ,  C07K7/06

CPC classification number: G01N33/6845 ,  C07K7/06 ,  C07K2319/00 ,  C12N15/1034 ,  C12N15/1044 ,  C12N15/1093

Abstract: Disclosed is a molecular library comprising a group of a plurality of molecules, wherein each member of the library is a polypeptide having a randomized sequence moiety and a microprotein moiety. The microprotein is a protein comprising an amino acid sequence of 30 or less amino acid residues having the ability to form a particular conformation by spontaneous folding in a solution and is, for example, chignolin comprising the amino acid sequence represented by SEQ ID NO: 1. Also, disclosed is a method for identifying a novel functional molecule using the library of the present invention.

公开(公告)号：US20190135860A1

公开(公告)日：2019-05-09

申请号：US16184028

申请日：2018-11-08

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Yukako Senga ,  Hideki Watanabe ,  Shinya Honda

IPC: C07K1/32 ,  C07K16/06 ,  C07K1/02 ,  C07K1/14 ,  C07K1/36

Abstract: The present invention relates to a method for suppressing aggregation of polypeptide. Specifically, the present invention relates to a method for suppressing, in a solution comprising an antibody or an Fc region-containing protein, formation of an aggregate derived from an antibody or an Fc region-containing protein having a non-native conformation, the method comprising: the steps of (i) binding an AF.2A1 polypeptide or an analog thereof with an aggregate derived from the antibody or Fc region-containing protein having a non-native conformation in the solution; and (ii) collecting the aggregate bound to the polypeptide or analog thereof from the solution.