-
公开(公告)号：US10941179B2
公开(公告)日：2021-03-09
申请号：US16184028
申请日：2018-11-08
Inventor： Yukako Senga , Hideki Watanabe , Shinya Honda
IPC: C07K1/22 , C07K16/00 , C07K1/14 , C07K1/02 , C07K14/00 , C07K7/06 , C07K7/08 , C07K1/32 , C07K16/06 , C07K1/36 , A61K39/395
Abstract: The present invention relates to a method for suppressing aggregation of polypeptide. Specifically, the present invention relates to a method for suppressing, in a solution comprising an antibody or an Fc region-containing protein, formation of an aggregate derived from an antibody or an Fc region-containing protein having a non-native conformation, the method comprising: the steps of (i) binding an AF.2A1 polypeptide or an analog thereof with an aggregate derived from the antibody or Fc region-containing protein having a non-native conformation in the solution; and (ii) collecting the aggregate bound to the polypeptide or analog thereof from the solution.
-
公开(公告)号：US09605029B2
公开(公告)日：2017-03-28
申请号：US14763589
申请日：2013-12-25
Inventor： Hideki Watanabe , Shinya Honda
CPC classification number: C07K14/001 , C07K7/06 , C07K7/08 , C07K16/4208 , G01N33/6854
Abstract: Polypeptides which have binding activity to an Fc region of immunoglobulin G and can be favorably used in detecting, purifying, immobilizing or removing an antibody, immunoglobulin G or a protein containing an Fc region of immunoglobulin G, are described. Methods for detecting, purifying, immobilizing or removing an antibody, immunoglobulin G or a protein containing an Fc region of immunoglobulin G, by using the peptide, are also described.
-
公开(公告)号：US11673929B2
公开(公告)日：2023-06-13
申请号：US16046276
申请日：2018-07-26
Inventor： Shinya Honda , Takamitsu Miyafusa
IPC: A61K38/22 , C07K14/535 , C07K1/02 , C12P21/02 , C12N5/10 , C07K19/00 , A61P7/00 , C12N15/70 , A61K38/00
CPC classification number: C07K14/535 , A61K38/22 , A61P7/00 , C07K1/02 , C07K19/00 , C12N5/10 , C12N15/70 , C12P21/02 , A61K38/00 , C12N2840/445
Abstract: The purpose of the present invention is to provide a method for producing a very stable, cyclized mutant protein such that high cyclization efficiency is achieved while the number of amino acids added is minimal and the biological properties of an original protein are maintained. In view of conformational information about the original protein, secondary structure-free regions at N/C terminal portions are deleted. Then, a protein database is screened for proteins with secondary structures similar to those of N/C terminal residues of a secondary structure-forming portion after the deletion. The screening results are used to determine the amino acid length of a loop structure through which the N-terminus and the C-terminus of the secondary structure-forming portion of the original protein are to be connected. A cyclized mutant protein is finally produced having a loop structure with the determined amino acid length.
-
4.发明授权公开(公告)号：US11008365B2
公开(公告)日：2021-05-18
申请号：US16329812
申请日：2017-08-31
Inventor： Hideki Watanabe , Shinya Honda
Abstract: The present invention relates to a novel polypeptide having affinity for proteins partially including a CH1-CL domain forming a non-native three-dimensional structure and capable of being suitably used for detecting, immobilizing, or removing these proteins and relates to use of the polypeptide. Specifically, disclosed are a polypeptide consisting of an amino acid sequence represented by any one of the following formulas 1 to 3: (1) P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 1), (2) Y-D-P-E-T-G-T-W-P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 4), and (3) P-N-S-G-G-G-G-S-Y-D-P-E-T-G-T-W-P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 7) (wherein x represents an amino acid residue; and brackets represent any one of the amino acid residues within the brackets), and a method of using the polypeptide to detect, purify, or remove a protein partially including a CH1-CL domain forming a non-native three-dimensional structure.
-
Patent Agency Ranking
公开(公告)号：US20180346539A1
公开(公告)日：2018-12-06
申请号：US16046276
申请日：2018-07-26
Inventor： Shinya Honda , Takamitsu Miyafusa
IPC: C07K14/535 , C12P21/02 , C07K19/00 , A61K38/22 , C12N15/70 , C12N5/10 , A61P7/00 , C07K1/02 , A61K38/00
CPC classification number: C07K14/535 , A61K38/00 , A61K38/22 , A61P7/00 , C07K1/02 , C07K19/00 , C12N15/70 , C12N2840/445 , C12P21/02
Abstract: The purpose of the present invention is to provide a method for producing a very stable, cyclized mutant protein such that high cyclization efficiency is achieved while the number of amino acids added is minimal and the biological properties of an original protein are maintained. In view of conformational information about the original protein, secondary structure-free regions at N/C terminal portions are deleted. Then, a protein database is screened for proteins with secondary structures similar to those of N/C terminal residues of a secondary structure-forming portion after the deletion. The screening results are used to determine the amino acid length of a loop structure through which the N-terminus and the C-terminus of the secondary structure-forming portion of the original protein are to be connected. A cyclized mutant protein is finally produced having a loop structure with the determined amino acid length.
-
公开(公告)号：US09897611B2
公开(公告)日：2018-02-20
申请号：US14655792
申请日：2013-12-09
Inventor： Shinya Honda , Hideki Watanabe , Kazuhiko Yamasaki
CPC classification number: G01N33/6845 , C07K7/06 , C07K2319/00 , C12N15/1034 , C12N15/1044 , C12N15/1093
Abstract: Disclosed is a molecular library comprising a group of a plurality of molecules, wherein each member of the library is a polypeptide having a randomized sequence moiety and a microprotein moiety. The microprotein is a protein comprising an amino acid sequence of 30 or less amino acid residues having the ability to form a particular conformation by spontaneous folding in a solution and is, for example, chignolin comprising the amino acid sequence represented by SEQ ID NO: 1. Also, disclosed is a method for identifying a novel functional molecule using the library of the present invention.
-
公开(公告)号：US20190135860A1
公开(公告)日：2019-05-09
申请号：US16184028
申请日：2018-11-08
Inventor： Yukako Senga , Hideki Watanabe , Shinya Honda
Abstract: The present invention relates to a method for suppressing aggregation of polypeptide. Specifically, the present invention relates to a method for suppressing, in a solution comprising an antibody or an Fc region-containing protein, formation of an aggregate derived from an antibody or an Fc region-containing protein having a non-native conformation, the method comprising: the steps of (i) binding an AF.2A1 polypeptide or an analog thereof with an aggregate derived from the antibody or Fc region-containing protein having a non-native conformation in the solution; and (ii) collecting the aggregate bound to the polypeptide or analog thereof from the solution.
-
8.发明申请NOVEL MODIFIED PROTEIN COMPRISING TANDEM-TYPE MULTIMER OF MUTANT EXTRACELLULAR DOMAIN OF PROTEIN G 审中-公开包含蛋白质G突变体细胞域的多态性的新型修饰蛋白公开(公告)号：US20140221613A1
公开(公告)日：2014-08-07
申请号：US14233015
申请日：2012-08-03
Inventor： Shinya Honda , Hiroyuki Matsumaru , Hideki Watababe , Chuya Yoshida
IPC: C07K14/315
CPC classification number: C07K14/315 , C07K1/22 , C07K2319/00 , C07K2319/70 , C12N9/88 , C12N15/62
Abstract: The purpose of the present invention is: to provide an excellent protein which is further reduced in the binding property to an Fc region of an immunoglobulin and/or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a protein containing an extracellular domain of wild-type protein G, and which still keeps a high antibody-binding activity in a neutral region; and to capture and collect an antibody readily using the protein without denaturating the antibody. The present invention relates to: a protein that is reduced in the binding property to an Fc region of an immunoglobulin and/or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a multimer comprising an extracellular domain of wild type one, which is a domain having a binding activity to a protein comprising an Fc region of immunoglobulin G, while keeping a high antibody-binding activity in a neutral region, and also has a binding activity to a protein comprising the Fc region of immunoglobulin G, wherein the protein comprises a tandem-type multimer of a mutant of the extracellular domain; and others.
Abstract translation: 本发明的目的是提供优异的蛋白质，其与弱酸性区域的Fc区的结合性进一步降低，并且与弱免疫球蛋白的Fab区的结合性相比，与 含有野生型蛋白G的细胞外结构域的蛋白质，并且在中性区域仍保持高的抗体结合活性; 并且使用蛋白质容易地捕获和收集抗体而不使抗体变性。 本发明涉及：与包含细胞外结构域的多聚体相比，在弱酸性区域中降低了与免疫球蛋白Fc区的结合性和/或与免疫球蛋白的Fab区的结合性的蛋白质 野生型1，其是与包含免疫球蛋白G的Fc区的蛋白质具有结合活性的结构域，同时在中性区域保持高抗体结合活性，并且还具有与包含Fc区的蛋白质的结合活性 的免疫球蛋白G，其中所述蛋白质包含细胞外结构域的突变体的串联型多聚体; 和别的。
-
公开(公告)号：US20180118799A1
公开(公告)日：2018-05-03
申请号：US15566365
申请日：2016-04-13
Inventor： Shinya Honda , Takamitsu Miyafusa
IPC: C07K14/535 , A61P7/00 , C12N15/70
CPC classification number: C07K14/535 , A61K38/00 , A61K38/22 , A61P7/00 , C07K1/02 , C07K19/00 , C12N5/10 , C12N15/70 , C12N2840/445 , C12P21/02
Abstract: The purpose of the present invention is to provide a method for producing a very stable, cyclized mutant protein such that high cyclization efficiency is achieved while the number of amino acids added is minimal and the biological properties of an original protein are maintained. In view of conformational information about the original protein, secondary structure-free regions at N/C terminal portions are deleted. Then, a protein database is screened for proteins with secondary structures similar to those of N/C terminal residues of a secondary structure-forming portion after the deletion. The screening results are used to determine the amino acid length of a loop structure through which the N-terminus and the C-terminus of the secondary structure-forming portion of the original protein are to be connected. A cyclized mutant protein is finally produced having a loop structure with the determined amino acid length.
-
-
-
-
-
-
-
-
Search Results
9
records
(took 0.025 seconds)
