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    METHOD FOR PRODUCTION OF BIOLOGICAL ORGANIC MATERIAL AND CULTURE VESSEL
    1.
    发明申请
    METHOD FOR PRODUCTION OF BIOLOGICAL ORGANIC MATERIAL AND CULTURE VESSEL 有权
    生物有机材料和文化船的生产方法

    公开(公告)号：US20090197333A1

    公开(公告)日：2009-08-06

    申请号：US11909909

    申请日：2006-03-29

    申请人： Nagahiro Saito ,  Osamu Takai ,  Yunying Wu ,  Hiroyuki Honda ,  Akira Ito

    发明人： Nagahiro Saito ,  Osamu Takai ,  Yunying Wu ,  Hiroyuki Honda ,  Akira Ito

    IPC分类号： C12N5/02 ,  C12M3/02

    CPC分类号： C12N5/0062 ,  C12M25/01

    摘要： Disclosed is a method for producing a biological organic material in a liquid droplet comprising a culture medium composed of specified ingredient. The method comprises the steps of: providing a substantially spherical liquid droplet on a substrate in an unadhered state, the substrate preferably having such a water-repellent surface that the water contact angle becomes 150° or greater; and culturing a biological organic material in the liquid droplet.

    摘要翻译： 本发明公开了一种生产有机材料的方法，该液滴包括由特定成分组成的培养基。 该方法包括以下步骤：在未粘附状态的基底上提供基本上球形的液滴，所述基底优选具有使水接触角变为150°以上的防水表面; 并在液滴中培养生物有机材料。

    Method for production of biological organic material and culture vessel
    2.
    发明授权
    Method for production of biological organic material and culture vessel 有权
    生物有机物和培养容器的生产方法

    公开(公告)号：US07951584B2

    公开(公告)日：2011-05-31

    申请号：US11909909

    申请日：2006-03-29

    申请人： Nagahiro Saito ,  Osamu Takai ,  Yunying Wu ,  Hiroyuki Honda ,  Akira Ito

    发明人： Nagahiro Saito ,  Osamu Takai ,  Yunying Wu ,  Hiroyuki Honda ,  Akira Ito

    IPC分类号： C12M1/12

    CPC分类号： C12N5/0062 ,  C12M25/01

    摘要： Disclosed is a method for producing a biological organic material in a liquid droplet comprising a culture medium composed of specified ingredient. The method comprises the steps of: providing a substantially spherical liquid droplet on a substrate in an unadhered state, the substrate preferably having such a water-repellent surface that the water contact angle becomes 150° or greater; and culturing a biological organic material in the liquid droplet.

    摘要翻译： 本发明公开了一种生产有机材料的方法，该液滴包括由特定成分组成的培养基。 该方法包括以下步骤：在未粘附状态的基底上提供基本上球形的液滴，所述基底优选具有使水接触角变为150°以上的防水表面; 并在液滴中培养生物有机材料。

    Production of cell culture product and material for use in said production
    3.
    发明授权
    Production of cell culture product and material for use in said production 有权
    生产用于所述生产的细胞培养产物和材料

    公开(公告)号：US07883865B2

    公开(公告)日：2011-02-08

    申请号：US11574949

    申请日：2005-09-08

    申请人： Nagahiro Saito ,  Osamu Takai ,  Yunying Wu ,  Hiroyuki Honda ,  Akira Ito

    发明人： Nagahiro Saito ,  Osamu Takai ,  Yunying Wu ,  Hiroyuki Honda ,  Akira Ito

    IPC分类号： C12P21/02

    CPC分类号： C12N5/069 ,  A61L27/3804 ,  A61L27/507 ,  A61L2430/36 ,  C12M23/20 ,  C12M25/01 ,  C12M25/06 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2535/10

    摘要： A base plate for use in cell culture which comprises a base material, a water-repellent layer having a water-repellent surface formed on the above base material and a hydrophilic surface having a prescribed pattern formed on the base material; a method for producing the above base plate; and a method for producing a cell culture product using the base plate. It is preferred that the water-repellent surface has a water contact angle of more than 150°. The above method for producing a cell culture product using the base plate base plate is a novel method which allows the production of a cell culture product of a fine pattern.

    摘要翻译： 一种用于细胞培养的基板，包括基材，在上述基材上形成有防水表面的防水层和形成在基材上的具有规定图案的亲水性表面; 制造上述基板的方法; 以及使用该基板制造细胞培养物的方法。 优选的是，防水面的水接触角大于150°。 上述使用基板底板的细胞培养物的制造方法是能够制造精细图案的细胞培养物的新方法。

    Production of Cell Culture Product and Material for Use in Said Production
    4.
    发明申请
    Production of Cell Culture Product and Material for Use in Said Production 有权
    生产用于所述生产的细胞培养产物和材料

    公开(公告)号：US20080032403A1

    公开(公告)日：2008-02-07

    申请号：US11574949

    申请日：2005-09-08

    申请人： Nagahiro Saito ,  Osamu Takai ,  Yunying Wu ,  Hiroyuki Honda ,  Akira Ito

    发明人： Nagahiro Saito ,  Osamu Takai ,  Yunying Wu ,  Hiroyuki Honda ,  Akira Ito

    IPC分类号： C12N5/02

    CPC分类号： C12N5/069 ,  A61L27/3804 ,  A61L27/507 ,  A61L2430/36 ,  C12M23/20 ,  C12M25/01 ,  C12M25/06 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2535/10

    摘要： A base plate for use in cell culture which comprises a base material, a water-repellent layer having a water-repellent surface formed on the above base material and a hydrophilic surface having a prescribed pattern formed on the base material; a method for producing the above base plate; and a method for producing a cell culture product using the base plate. It is preferred that the water-repellent surface has a water contact angle of more than 150°. The above method for producing a cell culture product using the base plate base plate is a novel method which allows the production of a cell culture product of a fine pattern.

    摘要翻译： 一种用于细胞培养的基板，包括基材，在上述基材上形成有防水表面的防水层和形成在基材上的具有规定图案的亲水性表面; 制造上述基板的方法; 以及使用该基板制造细胞培养物的方法。 优选的是，防水面的水接触角大于150°。 上述使用基板底板的细胞培养物的制造方法是能够制造精细图案的细胞培养物的新方法。

    Cell culture method and cell sheet
    5.
    发明申请
    Cell culture method and cell sheet 审中-公开
    细胞培养方法和细胞片

    公开(公告)号：US20060063252A1

    公开(公告)日：2006-03-23

    申请号：US11226247

    申请日：2005-09-15

    申请人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata ,  Hiroko Terasaki

    发明人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata ,  Hiroko Terasaki

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0629 ,  C12M25/00 ,  C12N5/0075 ,  C12N11/14 ,  C12N13/00

    摘要： Epidermal keratinocytes not incorporating magnetic particles therein and epidermal keratinocytes incorporating magnetic particles therein were seeded in a 24-well ultra-low-attachment plate, and cultured for one day after a neodymium magnet was placed outside the bottom of the well. As a result, control epidermal keratinocytes not incorporating magnetic particles therein did not adhere to the bottom of the well and did not form an undifferentiated cell sheet. On the contrary, epidermal keratinocytes incorporating magnetic particles therein, while being attracted to the bottom of the well by magnetic force, formed a multilayered cell sheet. This cell sheet could be easily recovered by removing the neodymium magnet placed outside the bottom of the well, then bringing a magnet having a hydrophilic film attached thereto close to the cell sheet from upside and to attract it via this hydrophilic film, and lifting it.

    摘要翻译： 将不含有磁性颗粒的表皮角化细胞和其中含有磁性颗粒的表皮角质形成细胞接种在24孔超低附着板中，并在将钕磁铁放置在孔底部之外培养1天。 结果，不含有磁性颗粒的对照表皮角化细胞不粘附到孔的底部，并且不形成未分化的细胞片。 相反，在其中通过磁力吸引到井底部的同时包含磁性颗粒的表皮角质形成细胞形成多层细胞片。 通过去除放置在孔底部的钕磁铁，可以容易地回收该细胞片，然后将从其上方附着有亲水膜的磁体附着到细胞片上，并通过该亲水性膜吸引，并将其提起。

    Cell culture method and cultured tissue
    6.
    发明申请
    Cell culture method and cultured tissue 审中-公开
    细胞培养法和培养组织

    公开(公告)号：US20060121606A1

    公开(公告)日：2006-06-08

    申请号：US11271911

    申请日：2005-11-14

    申请人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata

    发明人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0062

    摘要： A cell culture method prepares first cells that are adhesion-dependent cells and monolayered or multilayered cells on a culture surface of a culture substrate, seeds second cells that are adhesion-dependent cells and are magnetized by allowing to have magnetic particles on the first cells, induces the second cells to a predetermined position on the first cells by magnetic force, and cultures the first cells and the second cells in a cell arrangement obtained by the magnetic induction. According to this cell culture method, after a cell sheet was prepared individually, cells can be multilayered without changing a temperature, peeling the monolayered sheet and laminating the monolayered sheets.

    摘要翻译： 细胞培养方法在培养基底的培养表面上制备作为粘附依赖性细胞和单层或多层细胞的第一细胞，作为粘附依赖性细胞的种子第二细胞并通过在第一细胞上具有磁性颗粒而被磁化， 通过磁力将第二细胞诱导到第一细胞上的预定位置，并以通过磁感应获得的细胞排列来培养第一细胞和第二细胞。 根据该细胞培养方法，单独制备细胞片后，细胞可以多层而不改变温度，剥离单层片材并层压单层片材。