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公开(公告)号:US09856466B2
公开(公告)日:2018-01-02
申请号:US14115801
申请日:2012-05-04
申请人: Neelam S. Amin , Katherine Augustyn , Joshua R. Basler , Luis G. Cascao-Pereira , Katherine D. Collier , Edward M. Concar , David A. Estell , James T. Kellis, Jr. , Euan John Magennis , Alexander Pisarchik , Ayrookaran J. Poulose , Philip Frank Souter , Glenn Steven Ward , Jian Yao
发明人: Neelam S. Amin , Katherine Augustyn , Joshua R. Basler , Luis G. Cascao-Pereira , Katherine D. Collier , Edward M. Concar , David A. Estell , James T. Kellis, Jr. , Euan John Magennis , Alexander Pisarchik , Ayrookaran J. Poulose , Philip Frank Souter , Glenn Steven Ward , Jian Yao
摘要: The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.
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公开(公告)号:US08785171B2
公开(公告)日:2014-07-22
申请号:US12963681
申请日:2010-12-09
申请人: Philip Frank Souter , Glenn Steven Ward , Viktor Yuryevich Alekseyev , Joshua Roy Basler , Luis Gustavo Cascão-Pereira , David A. Estell , Ayrookaran J. Poulose , James T. Kellis, Jr. , Alexander Pisarchik , Daniel Esteban Torres-Pazmino
发明人: Philip Frank Souter , Glenn Steven Ward , Viktor Yuryevich Alekseyev , Joshua Roy Basler , Luis Gustavo Cascão-Pereira , David A. Estell , Ayrookaran J. Poulose , James T. Kellis, Jr. , Alexander Pisarchik , Daniel Esteban Torres-Pazmino
IPC分类号: C12N9/48
CPC分类号: C11D3/38618 , C11D3/38609 , C11D3/38681 , C12N9/50
摘要: This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN′ subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
摘要翻译: 本发明涉及包含一种或多种冷水蛋白酶的织物和家庭护理产品以及用于制造和使用这些产品的方法。 这样的组合物提供改善的清洁和新鲜度。 这样的冷水蛋白酶可以通过取代,插入和/或缺失一种或多种亲本氨基酸从母体酶衍生,包括BPN'枯草杆菌蛋白酶和源自迟缓芽孢杆菌的枯草杆菌蛋白酶。
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公开(公告)号:US08183024B2
公开(公告)日:2012-05-22
申请号:US12616106
申请日:2009-11-10
申请人: Luis G. Cascao-Pereira , David A. Estell , Frits Goedegebuur , James T. Kellis, Jr. , Ayrookaran J. Poulise
发明人: Luis G. Cascao-Pereira , David A. Estell , Frits Goedegebuur , James T. Kellis, Jr. , Ayrookaran J. Poulise
IPC分类号: C12N9/48
CPC分类号: C11D3/386 , C11D3/38681 , C11D17/0086
摘要: The present invention provides a subtilisin variant that is particularly well suited to cleaning applications. In particular, the present invention provides a Bacillus sp. subtilisin variant and cleaning compositions comprising this variant.
摘要翻译: 本发明提供了特别适合于清洁应用的枯草杆菌蛋白酶变体。 特别地,本发明提供芽孢杆菌属 枯草溶菌素变体和包含该变体的清洁组合物。
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公开(公告)号:US08753861B2
公开(公告)日:2014-06-17
申请号:US13128854
申请日:2009-11-10
CPC分类号: C12N9/54 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/38663 , C11D3/38681 , C12N15/52 , C12Y304/21062
摘要: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.
摘要翻译: 本发明提供工程蛋白酶变体。 特别地,蛋白酶变体在选定的表面位置包含影响酶的电荷和/或疏水性的可组合突变,以在所选择的应用中增强所得变体酶的至少一种所需性质。 还提供了包含蛋白酶变体的组合物及其使用方法。
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公开(公告)号:US07129076B2
公开(公告)日:2006-10-31
申请号:US10423649
申请日:2003-04-25
申请人: Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
发明人: Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
CPC分类号: A23G4/123 , A61K8/66 , A61K2800/86 , A61Q11/00 , C11D3/386 , C12N9/54 , C12Y304/21062
摘要: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease.Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease.Further provided is a method of producing a protease variant that is more effective in a low detergent concentration system, medium detergent concentration system and high detergent concentration system than a precursor protease.
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公开(公告)号:US06927055B2
公开(公告)日:2005-08-09
申请号:US10228572
申请日:2002-08-27
申请人: Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell , André C. Baeck
发明人: Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell , André C. Baeck
IPC分类号: A23G4/00 , A23G4/12 , A23K1/165 , A61K8/00 , A61K8/25 , A61K8/36 , A61K8/37 , A61K8/40 , A61K8/41 , A61K8/44 , A61K8/46 , A61K8/60 , A61K8/64 , A61K8/66 , A61Q1/02 , A61Q1/14 , A61Q11/00 , A61Q11/02 , A61Q19/00 , A61Q19/10 , C11D3/386 , C11D3/395 , C11D7/42 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/50 , C12N9/54 , C12N9/56 , C12N9/58 , C12N15/09 , C12N15/57 , C12R1/07 , C12N9/52
CPC分类号: C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.
摘要翻译: 公开了衍生自天然存在或重组非人蛋白酶的DNA序列的新型蛋白酶变体。 一般来说,变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得,以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体蛋白酶具有与前体蛋白酶不同的性质,例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置62,212,230,232,252和257。
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公开(公告)号:US06610642B2
公开(公告)日:2003-08-26
申请号:US09922678
申请日:2001-08-06
申请人: Chanchal Kumar Ghosh , André Cesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
发明人: Chanchal Kumar Ghosh , André Cesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
IPC分类号: C11D742
CPC分类号: C11D3/386
摘要: The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a subtitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
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公开(公告)号:US06376450B1
公开(公告)日:2002-04-23
申请号:US09529905
申请日:2000-04-20
申请人: Chanchal Kumar Ghosh , AndréCesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
发明人: Chanchal Kumar Ghosh , AndréCesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
IPC分类号: C11D300
CPC分类号: C11D3/386
摘要: The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of $(Bacillus amyloliquefaciens) subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
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公开(公告)号:US06312936B1
公开(公告)日:2001-11-06
申请号:US09178155
申请日:1998-10-23
申请人: Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell , André C. Baeck
发明人: Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell , André C. Baeck
IPC分类号: C12N950
CPC分类号: C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号:US06838425B2
公开(公告)日:2005-01-04
申请号:US10047689
申请日:2002-01-15
申请人: Chanchal Kumar Ghosh , André Cesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
发明人: Chanchal Kumar Ghosh , André Cesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
CPC分类号: C11D3/386
摘要: The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a subtitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
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