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8 条记录 (耗时 0.018 秒)

    Determination of methylation of nucleic acid sequences
    3.
    发明申请
    Determination of methylation of nucleic acid sequences 审中-公开
    确定核酸序列的甲基化

    公开(公告)号：US20060094016A1

    公开(公告)日：2006-05-04

    申请号：US10537186

    申请日：2003-12-02

    申请人： Niall Gormley

    发明人： Niall Gormley

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/6874 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00729 ,  C12Q1/6827 ,  C12Q2600/156 ,  C40B40/06 ,  C12Q2525/301 ,  C12Q2525/131 ,  C12Q2523/125 ,  C12Q2521/307

    摘要： The invention relates to a method of detecting the precise locations of methyl-cytosines in a given nucleic acid sequence. In particular, the invention features a method which includes sequencing a template nucleic acid that is attached to a hairpin nucleic acid or double-stranded nucleic acid anchor, which contain specifically-designed sites for nicking or other endonucleases. The template nucleic acid is then regenerated to single-stranded form via methods described herein, and then treated to convert either the methylated cytosines, or non-methylated cytosines, and the template nucleic acid is then re-sequenced The results of the first and second sequencing reactions are then compared.

    摘要翻译： 本发明涉及检测给定核酸序列中甲基胞嘧啶精确位置的方法。 特别地，本发明的特征在于一种方法，其包括测序连接到发卡核酸或双链核酸锚的模板核酸，其包含用于切口或其它内切核酸酶的特异设计的位点。 然后通过本文所述的方法将模板核酸再生成单链形式，然后处理以转化甲基化胞嘧啶或非甲基化胞嘧啶，然后重新测序模板核酸。第一和第二 然后比较测序反应。

    Method of target enrichment
    4.
    发明申请
    Method of target enrichment 审中-公开
    目标浓缩方法

    公开(公告)号：US20070141604A1

    公开(公告)日：2007-06-21

    申请号：US11599605

    申请日：2006-11-14

    申请人： Niall Gormley ,  John West

    发明人： Niall Gormley ,  John West

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2565/501 ,  C12Q2525/191

    摘要： The present invention is directed to a method for reducing the complexity of a nucleic acid sample in a reproducible manner by enriching for specific nucleic acid target sequences in the population of nucleic acids. More specifically, the invention relates to a method for enriching specific target sequences in a population using libraries of oligonucleotides.

    摘要翻译： 本发明涉及通过富集核酸群体中的特定核酸靶序列，以可再现的方式降低核酸样品的复杂性的方法。 更具体地，本发明涉及使用寡核苷酸文库丰富群体中特异性靶序列的方法。

    Recovery of original template
    5.
    发明申请
    Recovery of original template 审中-公开
    恢复原始模板

    公开(公告)号：US20060035233A1

    公开(公告)日：2006-02-16

    申请号：US10537188

    申请日：2003-12-02

    申请人： Niall Gormley ,  Shankar Balasubramanian

    发明人： Niall Gormley ,  Shankar Balasubramanian

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6837 ,  C12Q2525/301 ,  C12Q2521/301 ,  C12Q2521/313

    摘要： The present invention relates to methods for regenerating a single-stranded nucleic acid template following its conversion to a double-stranded product, e.g., during a polymerase reaction, and also to regenerating a hairpin or anchoring sequence by removal of the template and its synthesized complement, by design of enzyme restriction sites into the hairpin or anchoring sequence.

    摘要翻译： 本发明涉及在转化成双链产物之后，例如在聚合酶反应期间再生单链核酸模板的方法，还涉及通过除去模板及其合成的补体来再生发夹或锚定序列 通过将酶限制性位点设计到发夹或锚定序列中。

    Arrayed polynucleotides
    6.
    发明申请
    Arrayed polynucleotides 审中-公开
    阵列多核苷酸

    公开(公告)号：US20080108515A1

    公开(公告)日：2008-05-08

    申请号：US11895802

    申请日：2007-08-27

    申请人： Niall Gormley ,  Adrian Horgan ,  Darren Ellis ,  Jean-Ernest Sohna

    发明人： Niall Gormley ,  Adrian Horgan ,  Darren Ellis ,  Jean-Ernest Sohna

    IPC分类号： C40B50/06

    CPC分类号： C40B50/06 ,  C40B40/08

    摘要： The present invention is concerned with methods of producing fabricated arrays comprising clusters of clonal copies of one or more target molecules. In particular the invention relates to methods comprising providing clonal copies of a single target molecule within a vesicle or on the surface of a solid support within a chamber defined by a vesicle in contact with a solid support to produce clonal copies thereof and immobilising the copies on the solid support.

    摘要翻译： 本发明涉及制备包含一个或多个靶分子的克隆拷贝簇的制造阵列的方法。 特别地，本发明涉及包括提供单个靶分子在囊泡内或固体支持物表面上的克隆拷贝，所述固体支持物在由固体支持物接触的囊泡限定的室内，以产生其克隆拷贝，并将拷贝固定在 坚实的支持。

    Determination of methylation of nucleic acid sequences
    7.
    发明申请
    Determination of methylation of nucleic acid sequences 审中-公开
    确定核酸序列的甲基化

    公开(公告)号：US20080207460A1

    公开(公告)日：2008-08-28

    申请号：US12004224

    申请日：2007-12-20

    申请人： Niall Gormley

    发明人： Niall Gormley

    IPC分类号： C40B20/02 ,  C40B40/06

    CPC分类号： C12Q1/6874 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00729 ,  C12Q1/6827 ,  C12Q2600/156 ,  C40B40/06 ,  C12Q2525/301 ,  C12Q2525/131 ,  C12Q2523/125 ,  C12Q2521/307

    摘要： The invention relates to a method of detecting the precise locations of methyl-cytosines in a given nucleic acid sequence. In particular, the invention features a method which includes sequencing a template nucleic acid that is attached to a hairpin nucleic acid or double-stranded nucleic acid anchor, which contain specifically-designed sites for nicking or other endonucleases. The template nucleic acid is then regenerated to single-stranded form via methods described herein, and then treated to convert either the methylated cytosines, or non-methylated cytosines, and the template nucleic acid is then re-sequenced The results of the first and second sequencing reactions are then compared.

    摘要翻译： 本发明涉及检测给定核酸序列中甲基胞嘧啶精确位置的方法。 特别地，本发明的特征在于一种方法，其包括测序连接到发卡核酸或双链核酸锚的模板核酸，其包含用于切口或其它内切核酸酶的特异设计的位点。 然后通过本文所述的方法将模板核酸再生成单链形式，然后处理以转化甲基化胞嘧啶或非甲基化胞嘧啶，然后重新测序模板核酸。第一和第二 然后比较测序反应。