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1.发明申请Conjugates of the c domain of human gelatinase a and polyethylene glycol, methods of purification and uses thereof 审中-公开人类明胶酶a和聚乙二醇的c结构域的缀合物，其纯化方法和用途公开(公告)号：US20050153416A1
公开(公告)日：2005-07-14
申请号：US10505990
申请日：2003-02-28
CPC分类号： C12N9/6491 , A61K38/4886 , A61K47/60
摘要： There is provided a conjugate comprising the C domain of human gelatinase A (PEX domain, PEX) and one to three poly(ethylene glycol) group(s), said poly(ethylene glycol) group(s) having an overall weight of from about 10 to 40 kDa and being conjugated via primary amino group(s) of said PEX. The conjugates are useful as anticancer agents.
摘要翻译： 提供了包含人明胶酶A（PEX结构域，PEX）和一至三个聚（乙二醇）基团的C结构域的共轭物，所述聚（乙二醇）基团的总重量为约 10至40kDa，并通过所述PEX的伯氨基共轭。 缀合物可用作抗癌剂。
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2.发明授权Methods of inhibiting the binding of P-selectin to PSGL-1 with anti-P-selectin antibodies 有权用抗P-选择蛋白抗体抑制P-选择素与PSGL-1结合的方法公开(公告)号：US07824684B2
公开(公告)日：2010-11-02
申请号：US12768150
申请日：2010-04-27
申请人： Yvo Graus , Jacques Himber , Miranda Jansen-Molenaar , Dorothee Kling , Erhard Kopetzki , Paul Parren , Frank Rebers , Beat Steiner , Anne Stern , Pamela Strein , Kay-Gunnar Stubenrauch , Jan van de Winkel , Martine van Vugt
发明人： Yvo Graus , Jacques Himber , Miranda Jansen-Molenaar , Dorothee Kling , Erhard Kopetzki , Paul Parren , Frank Rebers , Beat Steiner , Anne Stern , Pamela Strein , Kay-Gunnar Stubenrauch , Jan van de Winkel , Martine van Vugt
IPC分类号： A61K39/395 , C07K16/28
CPC分类号： C07K16/2854 , C07K2317/21 , C07K2317/52 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92 , Y10S435/81
摘要： This invention relates to anti-P-selectin antibodies and, in particular, to anti-P-selectin antibodies and variants thereof that contain an Fc part derived from human origin and do not bind complement factor C1q. These antibodies have new and inventive properties causing a benefit for a patient suffering from critical limb ischemia or peripheral arterial occlusive disease (CLI/PAOD).
摘要翻译： 本发明涉及抗P-选择素抗体，特别涉及含有源自人源的Fc部分并且不结合补体因子C1q的抗P-选择素抗体及其变体。 这些抗体具有新的和本发明的性质，为患有严重肢体缺血或外周动脉闭塞性疾病（CLI / PAOD）的患者带来益处。
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3.发明申请公开(公告)号：US20090275126A1
公开(公告)日：2009-11-05
申请号：US12504717
申请日：2009-07-17
申请人： Yvo Graus , Erhard Kopetzki , Klaus-Peter Kuenkele , Olaf Mundigl , Paul Parren , Frank Rebers , Ralf Schumacher , Jan van de Winkel , Martine Vriesema-van Vugt
发明人： Yvo Graus , Erhard Kopetzki , Klaus-Peter Kuenkele , Olaf Mundigl , Paul Parren , Frank Rebers , Ralf Schumacher , Jan van de Winkel , Martine Vriesema-van Vugt
CPC分类号： C07K16/2863 , A61K2039/505 , C07K16/2896 , C07K2317/73
摘要： Antibodies against insulin like growth factor I receptor (IGF-IR), methods for their production, pharmaceutical compositions containing said antibodies, and uses for such antibodies are disclosed. Such antibodies are implicated in antitumor therapy.
摘要翻译： 公开了针对胰岛素样生长因子I受体（IGF-1R）的抗体，其制备方法，含有所述抗体的药物组合物及其用途。 这些抗体涉及抗肿瘤治疗。
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4.发明授权公开(公告)号：US07378503B2
公开(公告)日：2008-05-27
申请号：US10815449
申请日：2004-04-01
申请人： Yvo Graus , Erhard Kopetzki , Klaus-Peter Kuenkele , Olaf Mundigl , Paul Parren , Frank Rebers , Ralf Schumacher , Jan van de Winkel , Martine Vriesema-van Vugt
发明人： Yvo Graus , Erhard Kopetzki , Klaus-Peter Kuenkele , Olaf Mundigl , Paul Parren , Frank Rebers , Ralf Schumacher , Jan van de Winkel , Martine Vriesema-van Vugt
CPC分类号： C07K16/2863 , A61K2039/505 , C07K2317/21 , C07K2317/52 , C07K2317/56 , C07K2317/565
摘要： Antibodies which bind to IGF-IR and inhibit the binding of IGF-I and IGF-II to IGF-IR are characterized. These antibodies are implicated in anti-tumor therapy.
摘要翻译： 表征与IGF-1R结合并抑制IGF-I和IGF-II与IGF-1R结合的抗体。 这些抗体涉及抗肿瘤治疗。
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公开(公告)号：US20070117185A1
公开(公告)日：2007-05-24
申请号：US11583573
申请日：2006-10-19
申请人： Erhard Kopetzki
发明人： Erhard Kopetzki
CPC分类号： C07K14/005 , A61K38/162 , C07K16/00 , C07K16/2863 , C07K2317/50 , C07K2319/00 , C12N2740/16122
摘要： A method for the recombinant production of a heterologous polypeptide in a eukaryotic host cell is described. The host cell comprises an expression plasmid, whereby the expression plasmid comprises in a 5′ to 3′ direction a) a promoter, b) a nucleic acid encoding a first polypeptide, whose amino acid sequence is selected from Table 1 depending on the first two amino acids of the second polypeptide, c) a nucleic acid encoding a second polypeptide comprising a nucleic acid encoding a heterologous polypeptide, a nucleic acid encoding a linker, and a nucleic acid encoding an immunoglobulin fragment, and d) a 3′ untranslated region comprising a polyadenylation signal. Further a plasmid and a kit are described.
摘要翻译： 描述了在真核宿主细胞中重组产生异源多肽的方法。 宿主细胞包含表达质粒，其中表达质粒包含5'至3'方向a）启动子，b）编码第一多肽的核酸，其氨基酸序列选自表1，取决于前两个 第二多肽的氨基酸，c）编码包含编码异源多肽的核酸，编码接头的核酸和编码免疫球蛋白片段的核酸的第二多肽的核酸，以及d）3'非翻译区，其包含 多聚腺苷酸化信号。 此外，还描述了质粒和试剂盒。
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公开(公告)号：US07034119B2
公开(公告)日：2006-04-25
申请号：US10866567
申请日：2004-06-12
IPC分类号： C07K1/00
CPC分类号： C12P21/02
摘要： A method for the recombinant production of a polypeptide by expressing a nucleic acid encoding said polypeptide in a microbial host cell, forming in the cytoplasm of said host cell inclusion bodies containing said polypeptide, and isolating, solubilizing and naturing said polypeptide, characterized in that after fermentation the host cell or the host cell content is incubated at a temperature of 40° C. or higher for at least 10 minutes and subsequently insoluble polypeptide is isolated from the host cell, this method providing an improved yield in inclusion bodies containing the desired polypeptide.
摘要翻译： 一种通过在微生物宿主细胞中表达编码所述多肽的核酸重组产生多肽的方法，在包含所述多肽的所述宿主细胞包涵体的细胞质中形成，并分离，增溶和变性所述多肽，其特征在于， 将宿主细胞或宿主细胞含量发酵在40℃或更高的温度下孵育至少10分钟，随后从宿主细胞中分离不溶性多肽，该方法在含有所需多肽的包涵体中提供改善的产量 。
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公开(公告)号：US06660492B1
公开(公告)日：2003-12-09
申请号：US09664595
申请日：2000-09-18
IPC分类号： C12Q137
CPC分类号： C12N9/6424 , C07K2319/00 , C12N9/6427 , C12N9/6432 , C12N9/6467 , C12Y304/21006
摘要： A chimeric serine protease whose protease domain is composed of two domain halves (half-sides) with a &bgr;-folded sheet structure, wherein the first domain half corresponds to the first domain half of a first serine protease and the second domain half corresponds to the second domain half of a second serine protease, has improved properties and can be readily crystallized.
摘要翻译： 一种嵌合丝氨酸蛋白酶，其蛋白酶结构域由具有β-折叠片状结构的两个半结构域（半侧）组成，其中第一结构域半部分对应于第一丝氨酸蛋白酶的第一半结构域半部分，第二结构域半部分对应于 第二丝氨酸蛋白酶的第二结构域半数具有改善的性质并且可以容易地结晶。
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公开(公告)号：US5705616A
公开(公告)日：1998-01-06
申请号：US461593
申请日：1995-06-05
申请人： Ludwig Lehle , Klaus Lehnert , Erhard Kopetzki
发明人： Ludwig Lehle , Klaus Lehnert , Erhard Kopetzki
IPC分类号： C07H21/04 , C07K14/395 , C12N1/16 , C12N1/19 , C12N9/04 , C12N15/00 , C12N15/09 , C12P21/00 , C12P21/02 , C12R1/865 , C07K1/00 , C12N1/14 , C12N1/18
CPC分类号： C12N9/0006 , C12N1/16 , C12P21/005 , C12R1/85
摘要： Saccharomyces mutants with defects in N-glycosylation which are obtainable by �.sup.3 H!-mannose suicide selection, introduction of one or several selective markers, selection of those strains which, after transformation with the plasmid YEpL/glucose oxidase, secrete 10 mg/l GOD or more into the culture medium after culture under standard conditions, are allelic to the ngd mutations in Saccharomyces cerevisiae, DSM 7042, DSM 7338, DSM 7160 and/or 7340 and express proteins with a uniform carbohydrate structure.
摘要翻译： 具有N-糖基化缺陷的糖酵母突变体，其可以通过[3 H] - 甘露糖自杀选择获得，引入一个或几个选择性标记，选择在用质粒YEpL /葡萄糖氧化酶转化后分泌10mg / l GOD的那些菌株 或更多的培养基在标准条件下培养后，等同于酿酒酵母，DSM 7042，DSM 7338，DSM 7160和/或7340中的ngd突变，并表达具有均匀碳水化合物结构的蛋白质。
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公开(公告)号：US10280214B2
公开(公告)日：2019-05-07
申请号：US13464878
申请日：2012-05-04
摘要： Herein are reported glycosylated repeat-motif-molecule conjugate of the following formula: (repeat-motif-molecule−linkern)m−conjugation partner−(linkero−repeat-motif-molecule)p, wherein n and o are independently of each other and independently for each value of m and p integer values of 0 or 1, and m and p are independently of each other integer values of 0 or 1 or 2 or 3 or 4 or 5 or 6 or 7, and wherein the repeat-motif-molecule conjugate comprises at least one oligosaccharide attached to a glycosylation site. Also reported are encoding nucleic acids and method for producing these repeat-motif-conjugates in mammalian cells.
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公开(公告)号：US08877895B2
公开(公告)日：2014-11-04
申请号：US13217537
申请日：2011-08-25
CPC分类号： C12N15/62 , C07K14/775 , C07K2319/02 , C07K2319/21 , C07K2319/50 , C07K2319/70 , C12N15/70 , C12P21/02 , C12P21/06
摘要： A pro-polypeptide which is useful for the expression of a polypeptide of interest in a prokaryotic cell. Therefore the pro-polypeptide is fused to the N-terminus of the polypeptide of interest. The pro-polypeptide as reported herein provides for improved expression yields and improves the handling of the fusion polypeptide (downstream processing, purification). For example, efficient endotoxin removal is effected while the protein of interest comprising the pro-polypeptide is bound e.g. to an affinity chromatography material. Thereafter the pro-polypeptide can efficiently be cleaved from the polypeptide of interest by the incorporated protease cleavage site with the cognate protease.
摘要翻译： 可用于在原核细胞中表达目的多肽的原多肽。 因此，原多肽与感兴趣的多肽的N末端融合。 本文报道的前多肽提供改善的表达产率并改善融合多肽的处理（下游处理，纯化）。 例如，有效的内毒素去除被实现，而包含前多肽的目的蛋白质被结合例如。 至亲和层析材料。 此后，通过与同源蛋白酶合并的蛋白酶切割位点，可以有效地从所述多肽切割所述多肽。
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