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公开(公告)号：US20180355308A1
公开(公告)日：2018-12-13
申请号：US16105625
申请日：2018-08-20
Applicant: Osaka University , Toppan Printing Co., Ltd.
Inventor： Michiya Matsusaki , Shinji Irie , Shiro Kitano
CPC classification number: C12N5/0062 , C12N5/0656 , C12N5/0693 , C12N2501/91 , C12N2501/998 , C12N2513/00
Abstract: A method of producing a three-dimensional cell tissue, including: a step A of mixing cells with a cationic substance and an extracellular matrix component to obtain a mixture; a step B of gathering the cells from the obtained mixture to form a cell aggregate on a substrate; and a step C of culturing the cells to obtain a three-dimensional cell tissue.
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公开(公告)号：US11179495B2
公开(公告)日：2021-11-23
申请号：US16480114
申请日：2018-01-31
Applicant: TOPPAN PRINTING CO., LTD. , OSAKA UNIVERSITY
Inventor： Michiya Matsusaki , Shinji Irie , Shiro Kitano
Abstract: Disclosed is a three-dimensional tissue comprising cells and collagen including endogenous collagen, wherein at least a portion of the cells is adhered to the collagen, and the content of the collagen is from 10 wt % to 90 wt % based on the three-dimensional tissue.
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公开(公告)号：US11254917B2
公开(公告)日：2022-02-22
申请号：US16104533
申请日：2018-08-17
Applicant: TOPPAN PRINTING CO., LTD. , OSAKA UNIVERSITY
Inventor： Kei Tsukamoto , Shinji Irie , Michiya Matsusaki
Abstract: A method of promoting spheroid formation, including: a preparation step of preparing a mixture obtained by mixing a cell sample with a promoter; and a culture step of culturing, inside a spheroid formation-culture vessel, the mixture obtained in the preparation step, in which the promoter is a polymer in which one or more selected from the group consisting of D-glucosamine, D-galactosamine, D-glucuronic acid, L-iduronic acid, and D-galactose are polymerized.
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Patent Agency Ranking
公开(公告)号：US20160251626A1
公开(公告)日：2016-09-01
申请号：US15028204
申请日：2014-10-09
Inventor： Mitsuru Akashi , Michiya Matsusaki , Yoshihiro Ishikawa , Utako Yokoyama
IPC: C12N5/077
CPC classification number: C12N5/0661 , C12N2513/00 , C12N2533/52 , C12N2533/54
Abstract: Provided are an elastic three-dimensional tissue and a method by which the tissue can be produced. The elastic three-dimensional tissue includes smooth muscle cells and an extracellular matrix component, with the smooth muscle cells being layered with the extracellular matrix component interposed therebetween. Furthermore, the production method for a three-dimensional tissue includes layering smooth muscle cells with an extracellular matrix component interposed therebetween, wherein the smooth muscle cells are those directed towards a differentiated type from an undifferentiated type.
Abstract translation: 提供了一种弹性三维组织和可以产生组织的方法。 弹性三维组织包括平滑肌细胞和细胞外基质组分，其中平滑肌细胞与介于其间的细胞外基质成分分层。 此外，用于三维组织的制备方法包括将平滑肌细胞分层插入其中的细胞外基质成分，其中平滑肌细胞是指向未分化型的分化型细胞。
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公开(公告)号：US11473054B2
公开(公告)日：2022-10-18
申请号：US15998571
申请日：2017-01-31
Applicant: OSAKA UNIVERSITY
Inventor： Michiya Matsusaki
Abstract: Provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (a) a step for forming a vascular system structure template using a gel; (b) a step for forming a three-dimensional tissue in the vicinity of the template; (c) a step for dissolving the template using a cationic solution; and (d) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells in a void remaining after the dissolution of the template. Also provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (i) a step for forming a vascular system structure template using a gel; (ii) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells on the template; (iii) a step for forming a three-dimensional tissue in the vicinity of the cells seeded above; and (iv) a step for dissolving the template using a cationic solution. Also provided is a three-dimensional tissue comprising a gel which has a vascular system structure.
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公开(公告)号：US10799615B2
公开(公告)日：2020-10-13
申请号：US15533724
申请日：2015-12-07
Applicant: OSAKA UNIVERSITY , TOHOKU UNIVERSITY
Inventor： Mitsuru Akashi , Michiya Matsusaki , Michiaki Unno , Naoaki Sakata , Gumpei Yoshimatsu
IPC: A61L27/38 , A61K35/44 , A61K35/39 , A61L27/36 , C12N5/071 , A61L27/60 , A61K35/28 , A61K35/36 , C12N5/00
Abstract: An artificial tissue containing pancreatic islet cells, fibroblasts and/or cells capable of differentiating, extracellular matrix, and vascular endothelial cells, in which the fibroblasts and/or the cells capable of differentiating, the extracellular matrix, and the vascular endothelial cells constitute a three-dimensional tissue structure in which a vascular network structure has been formed, and in which the three-dimensional tissue structure contains pancreatic islets constituted by aggregating ten or more of the pancreatic islet cells.
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7.发明申请ARTIFICIAL SKIN TISSUE, ARTIFICIAL SKIN MODEL AND MANUFACTURING METHOD THEREFOR 审中-公开人造皮肤组织，人造皮肤模型及其制造方法公开(公告)号：US20150250925A1
公开(公告)日：2015-09-10
申请号：US14426013
申请日：2013-09-04
Applicant: OSAKA UNIVERSITY , BioMedical Technology HYBRID Co., Ltd. , NATIONAL UNIVERSITY CORPORATION EHIME UNIVERSITY
Inventor： Mitsuru Akashi , Michiya Matsusaki , Masaaki Nawano , Koji Hashimoto , Yuji Shirakata
CPC classification number: A61L27/60 , A61F2210/0076 , A61L27/3633 , A61L27/383 , A61L2430/34 , C12N5/0639 , C12N5/0698 , C12N2501/052 , C12N2502/09 , C12N2502/1121 , C12N2533/54
Abstract: Provided is a novel method capable of manufacturing an artificial skin model including dendritic cells. A method for manufacturing an artificial skin model includes the following: forming a dermal tissue layer by culturing coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component, so that the coated cells are layered; forming a basal layer including type IV collagen on the dermal tissue layer by bringing type IV collagen into contact with the dermal tissue layer; and forming an epidermal layer by arranging epidermal cells on the basal layer. At least one of the dermal tissue layer and the epidermal layer includes dendritic cells.
Abstract translation: 提供了能够制造包括树突细胞的人造皮肤模型的新方法。 制造人造皮肤模型的方法包括：通过培养其中细胞表面涂覆有包含细胞外基质组分的涂膜的涂覆细胞，形成真皮组织层，使得涂覆的细胞层叠; 通过使IV型胶原与真皮组织层接触，在真皮组织层上形成包括IV型胶原蛋白的基底层; 并通过在基底层上布置表皮细胞形成表皮层。 皮肤组织层和表皮层中的至少一个包括树突状细胞。
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公开(公告)号：US20150247118A1
公开(公告)日：2015-09-03
申请号：US14427536
申请日：2013-09-12
Applicant: OSAKA UNIVERSITY , MITSUBISHI PAPER MILLS LIMITED
Inventor： Mitsuru Akashi , Michiya Matsusaki , Atsushi Matsuzawa
IPC: C12N5/00
CPC classification number: C12N5/0062 , C12N5/0671 , C12N2533/52 , C12N2533/90
Abstract: Provided is a novel method for producing a three-dimensional cell culture construct. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; culturing the seeded coated cells in a culture medium; and using at least a portion of the culture medium continuously for 5 days or more. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; and culturing the seeded coated cells in a culture medium. The culture process of the coated cells includes culturing the coated cells in 70 μL or more of the culture medium per 1×104 cells.
Abstract translation: 提供了一种用于生产三维细胞培养构建体的新方法。 包含至少两层以上的层叠单元层的三维细胞培养构建体的制造方法包括以下步骤：将含有细胞外基质成分的涂膜对细胞表面进行接种的涂覆细胞， 在培养基中培养接种的包被细胞; 并将培养基的至少一部分连续使用5天以上。 包含至少两层以上的层叠单元层的三维细胞培养构建体的制造方法包括以下步骤：将含有细胞外基质成分的涂膜对细胞表面进行接种的涂覆细胞， 并在培养基中培养接种的包被的细胞。 包被细胞的培养过程包括每1×10 4个细胞培养70μL以上的培养基。
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