公开(公告)号：US06420171B1

公开(公告)日：2002-07-16

申请号：US09549493

申请日：2000-04-14

申请人： Osamu Nakamura ,  Hideki Ohba ,  Imre Sallay ,  Fumio Yagi ,  Sawako Moriwaki ,  Seiji Yasuda

发明人： Osamu Nakamura ,  Hideki Ohba ,  Imre Sallay ,  Fumio Yagi ,  Sawako Moriwaki ,  Seiji Yasuda

IPC分类号： C12N506

CPC分类号： C12N5/0093 ,  A61M1/3679 ,  A61M2202/0439 ,  C07K14/37 ,  C07K14/42 ,  C07K17/10 ,  Y10S530/812 ,  Y10S530/813

摘要： An efficient method for therapeutic treatment of leukemia is provided in which a patient's body fluid during external circulation is brought into direct contact with an adsorbent material capable of specifically and selectively adsorbing leukemic cells in the body fluid. The leukemic cell-adsorbing material is a composite of a lectin protein coupled with a physiologically inert carrier material such as a galactan polysaccharide in the form of beads. The lectin protein may be obtained from a mushroom fungus such as Agrocybe cylindracea or a leguminous seed such as from the jequirity bean plant. The lectin protein and carrier material can be bound by forming chemical linkages between amino groups in the lectin protein and functional groups in the carrier material, and unreacted functional groups of the carrier material may be blocked with an amino acid. A leukemic cell-adsorbing column may be formed by filling the leukemic cell-adsorbing material into a tubular body to form an adsorbent bed. The tubular body may have an inner diameter of from 10 to 20 mm and a height of from 50 to 200 mm.

摘要翻译： 提供了一种治疗白血病的有效方法，其中外部循环期间的患者体液与能够特异性和选择性地吸收体液中的白血病细胞的吸附材料直接接触。 白血病细胞吸附材料是凝集素蛋白与生理惰性载体材料如珠粒形式的半乳聚糖多糖的复合物。 凝集素蛋白质可以从蘑菇真菌如花椰菜（Agrocybe cylindracea）或豆科植物种子（例如来自不育豆）得到。 凝集素蛋白质和载体材料可以通过在凝集素蛋白中的氨基和载体材料中的官能团之间形成化学键而结合，载体材料的未反应的官能团可以用氨基酸封闭。 可以通过将白血病细胞吸附材料填充到管状体中以形成吸附床来形成白血病细胞吸附柱。 管状体的内径可以为10〜20mm，高度为50〜200mm。

公开(公告)号：US06987022B2

公开(公告)日：2006-01-17

申请号：US10158157

申请日：2002-05-31

申请人： Osamu Nakamura ,  Hideki Ohba ,  Imre Sallay ,  Fumio Yagi ,  Sawako Moriwaki ,  Seiji Yasuda

发明人： Osamu Nakamura ,  Hideki Ohba ,  Imre Sallay ,  Fumio Yagi ,  Sawako Moriwaki ,  Seiji Yasuda

IPC分类号： C12N5/06 ,  C12N5/08 ,  C12N11/10 ,  C07K17/02 ,  C07K17/10

CPC分类号： C12N5/0093 ,  A61M1/3679 ,  A61M2202/0439 ,  C07K14/37 ,  C07K14/42 ,  C07K17/10 ,  C12N5/0694 ,  Y10S530/812 ,  Y10S530/813

摘要： An efficient method for therapeutic treatment of a leukemic patient is disclosed in which the patient's body fluid under external circulation is brought into direct contact with an adsorbent material capable of adsorbing the leukemic cells in the body fluid specifically and selectively. The leukemic cell-adsorbent material, which is used by filling a column to form an adsorbent bed, is a conjugate of a lectin protein extracted from, e.g., Dolichos beans or soybeans coupled with a physiologically inert carrier material such as a polysaccharide in the form of beads or a superparamagnetic material in the form of iron oxide-based magnetic beads.

摘要翻译： 公开了一种用于治疗白血病患者的有效方法，其中将外部循环下的患者体液与能够特异性且选择性地吸收体液中的白血病细胞的吸附材料直接接触。 用于填充柱以形成吸附床的白血病细胞吸附材料是从例如Dolichos豆或大豆与生理惰性载体材料如多糖形式的提取的凝集素蛋白的缀合物，其形式为 的珠子或以铁氧化物基磁珠形式的超顺磁性材料。

公开(公告)号：US6143533A

公开(公告)日：2000-11-07

申请号：US525323

申请日：2000-03-13

申请人： Toshihisa Sumi ,  Hideki Ohba ,  Toru Ikegami ,  Masao Shibata ,  Tsuyoshi Sakaki ,  Imre Sallay ,  Sung Soo Park

发明人： Toshihisa Sumi ,  Hideki Ohba ,  Toru Ikegami ,  Masao Shibata ,  Tsuyoshi Sakaki ,  Imre Sallay ,  Sung Soo Park

IPC分类号： C07H7/027 ,  A61K35/56 ,  C07H1/08 ,  C12P7/58 ,  C12P7/40 ,  A61K35/12

CPC分类号： C12P7/58

摘要： Disclosed is an efficient and economical method for the preparation of N-glycolyl neuraminic acid in a high purity from an inexpensive abundant source material. The method comprises dispersing body tissues of an echinodermatous marine animal Cucumaria echinata in an aqueous medium, preferably, using a dry powder of the tissues prepared in advance, in which the tissues are proteolytically decomposed to isolate N-glycolyl neuraminic acid in the form of an aqueous solution containing polypeptides as a by-product, followed by separation of N-glycolyl neuraminic acid from the aqueous solution by removing the polypeptides and purification of the compound in a process utilizing an ion-exchange treatments.

摘要翻译： 公开了一种从廉价丰富的原料制备高纯度的N-羟乙酰神经氨酸的有效和经济的方法。 该方法包括将棘皮动物海洋动物黄瓜（Cucumaria echinata）的身体组织分散在水性介质中，优选使用预先制备的组织的干粉末，其中组织被蛋白水解分解以分离出N-羟乙酰神经氨酸 水溶液，其含有作为副产物的多肽，随后通过在使用离子交换处理的方法中除去多肽和化合物的纯化从水溶液中分离N-羟乙酰神经氨酸。

公开(公告)号：US06342367B1

公开(公告)日：2002-01-29

申请号：US09513191

申请日：2000-02-25

申请人： Toshihisa Sumi ,  Hideki Ohba ,  Toru Ikegami ,  Masao Shibata ,  Tsuyoshi Sakaki ,  Imre Sallay ,  Sung Soo Park

发明人： Toshihisa Sumi ,  Hideki Ohba ,  Toru Ikegami ,  Masao Shibata ,  Tsuyoshi Sakaki ,  Imre Sallay ,  Sung Soo Park

IPC分类号： C12P2106

CPC分类号： C12P19/26

摘要： Disclosed is an economical method for the preparation of chondroitin sulfates A and C useful as an effective ingredient of medicaments from fish scales as a waste material discharged from fishery in large quantities. Fish scales are enzymatically decomposed in an aqueous medium in the presence of a protease to isolate the chondroitin sulfate compounds and by-product polypeptides followed by removal of the by-product polypeptides from the aqueous solution by a cation-exchange treatment and then the aqueous solution of the chondroitin sulfate compounds is subjected to fractional precipitation by the addition of ethyl alcohol as the precipitant.

摘要翻译： 公开了一种用于制备硫酸软骨素A和C的经济方法，其用作作为大量从渔业排放的废物的鱼鳞的药物的有效成分。 鱼鳞在蛋白酶存在下在水性介质中酶促分解，分离出硫酸软骨素化合物和副产物多肽，然后通过阳离子交换处理从水溶液中除去副产物多肽，然后将水溶液 的硫酸软骨素化合物通过加入乙醇作为沉淀剂进行分级沉淀。