公开(公告)号：US20180119081A1

公开(公告)日：2018-05-03

申请号：US15566566

申请日：2016-04-08

Applicant: President and Fellows of Harvard College

Inventor： Karel Domansky ,  Christopher David Hinojosa ,  Donald E. Ingber ,  Daniel Levner ,  Guy Thompson, II

IPC: C12M3/06 ,  C12M1/00 ,  C12M1/12

CPC classification number: C12M23/16 ,  B01L3/5027 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  C12M25/02 ,  C12M29/14 ,  C12M35/04 ,  C12M35/08 ,  C12M41/00

Abstract: A microfluidic device for determining a response of cells comprises a microchannel and a seeding channel. The microchannel is at least partially defined by a porous membrane having cells adhered thereto. The microchannel has a first cross-sectional area. The seeding channel delivers a working fluid to the cells within the microchannel. The seeding channel has a second cross-sectional area that is less than the first cross-sectional area such that a flow of the working fluid produces a substantially higher shear force within the seeding channel to inhibit the attachment of cells within the seeding channel. And when multiple seeding channels are used to deliver fluids to multiple microchannels that define an active cellular layer across the membrane, the seeding channels are spatially offset from each other such that fluid communication between the fluids occurs only at the active region via the membrane, not at the seeding channels.

公开(公告)号：US20240084235A1

公开(公告)日：2024-03-14

申请号：US18470404

申请日：2023-09-19

Applicant: President and Fellows of Harvard College

Inventor： Jose Fernandez-Alcon ,  Norman Wen ,  Richard Novak ,  Donald E. Ingber ,  Geraldine A. Hamilton ,  Christopher Hinojosa ,  Karel Domansky ,  Daniel Levner ,  Guy Thompson, II ,  Kambez Hajipouran Benam ,  Remi Villenave ,  Thomas Umundum ,  Alfred Paris ,  Georg Bauer

IPC: C12M3/06 ,  B01D61/18 ,  B01D63/08 ,  B01D67/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M1/42 ,  C12M3/00

CPC classification number: C12M23/16 ,  B01D61/18 ,  B01D63/081 ,  B01D67/0023 ,  B01L3/5027 ,  B01L3/502753 ,  C12M21/08 ,  C12M23/26 ,  C12M25/02 ,  C12M35/04 ,  C12M41/00 ,  C12M41/46 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2400/0487

Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

公开(公告)号：US11001797B2

公开(公告)日：2021-05-11

申请号：US14391995

申请日：2013-04-15

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Karel Domansky ,  Michael Karpelson ,  Donald E. Ingber

IPC: C12M1/00 ,  A61M11/00 ,  B05B11/00

Abstract: A system and methods for aerosol delivery of an entity or agent are disclosed. The system and methods can include a target application surface. A nebulizer can be located in close proximity to the target application surface. The nebulizer can include a chamber to hold the entity, a nozzle plate including one nozzle, and a piezoelectric element coupled to the nozzle plate. A power source can be coupled to the piezoelectric element. The power source, when activated, can energize the piezoelectric element to vibrate the nozzle plate to cause the entity to be nebulized through the nozzle to impact the target application surface.

公开(公告)号：US20160326477A1

公开(公告)日：2016-11-10

申请号：US15105388

申请日：2014-12-19

Applicant: Jose FERNANDEZ-ALCON ,  Norman WEN ,  Richard NOVAK ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Jose Fernandez-Alcon ,  Norman Wen ,  Richard Novak ,  Donald E. Ingber ,  Geraldine A. Hamilton ,  Christopher Hinojosa ,  Karel Domansky ,  Daniel Levner ,  Guy Thompson, II ,  Kambez Hajipouran Benam ,  Remi Villenave ,  Thomas Umundum ,  Alfred Paris ,  Georg Bauer

IPC: C12M3/06 ,  C12M1/12 ,  C12M1/00 ,  C12M1/42 ,  C12M1/34

CPC classification number: C12M23/16 ,  C12M21/08 ,  C12M23/26 ,  C12M25/02 ,  C12M35/04 ,  C12M41/00 ,  C12M41/46

Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

Abstract translation: 有机模拟装置包括可用于在其微流体通道中培养细胞的微流体装置。 有机模拟装置可以是动态系统的一部分，其可以通过调节微流体装置和通过微流体通道的流体流而将机械力施加到细胞。 有机模拟装置中的膜可以通过气动装置和/或机械装置机械调节。 有机模拟装置可以通过单独制造各个组件来制造，例如，作为随后层压在一起的各个层。

公开(公告)号：US20150079670A1

公开(公告)日：2015-03-19

申请号：US14391995

申请日：2013-04-15

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Karel Domansky ,  Michael Karpelson ,  Donald E. Ingber

IPC: C12M1/00 ,  B05B11/00

CPC classification number: C12M29/06 ,  A61M11/005 ,  A61M2205/0244 ,  B05B11/3028

Abstract: A system and methods for aerosol delivery of an entity or agent are disclosed. The system and methods can include a target application surface. A nebulizer can be located in close proximity to the target application surface. The nebulizer can include a chamber to hold the entity, a nozzle plate including one nozzle, and a piezoelectric element coupled to the nozzle plate. A power source can be coupled to the piezoelectric element. The power source, when activated, can energize the piezoelectric element to vibrate the nozzle plate to cause the entity to be nebulized through the nozzle to impact the target application surface.

Abstract translation: 公开了用于实体或代理的气溶胶递送的系统和方法。 系统和方法可以包括目标应用程序表面。 雾化器可以靠近目标应用表面。 喷雾器可以包括用于保持实体的腔室，包括一个喷嘴的喷嘴板和联接到喷嘴板的压电元件。 电源可以耦合到压电元件。 电源被激活时，能激励压电元件使喷嘴板振动，使得实体通过喷嘴雾化，以撞击目标应用表面。

公开(公告)号：US20170327781A1

公开(公告)日：2017-11-16

申请号：US15601430

申请日：2017-05-22

Applicant: President and Fellows of Harvard College

Inventor： Jose Fernandez-Alcon ,  Norman Wen ,  Richard Novak ,  Donald E. Ingber ,  Geraldine A. Hamilton ,  Christopher Hinojosa ,  Karel Domansky ,  Daniel Levner ,  Guy Thompson ,  Kambez Hajipouran Benam ,  Remi Villenave ,  Thomas Umundum ,  Alfred Paris ,  Georg Bauer

IPC: C12M3/06 ,  C12M1/42 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34

CPC classification number: C12M23/16 ,  C12M21/08 ,  C12M23/26 ,  C12M25/02 ,  C12M35/04 ,  C12M41/00 ,  C12M41/46

Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

公开(公告)号：US12173263B2

公开(公告)日：2024-12-24

申请号：US15105388

申请日：2014-12-19

Applicant: President and Fellows of Harvard College ,  Jose Fernandez-Alcon ,  Norman Wen ,  Richard Novak

Inventor： Jose Fernandez-Alcon ,  Norman Wen ,  Richard Novak ,  Donald E. Ingber ,  Geraldine A. Hamilton ,  Christopher Hinojosa ,  Karel Domansky ,  Daniel Levner ,  Guy Thompson, II ,  Kambez Hajipouran Benam ,  Remi Villenave ,  Thomas Umundum ,  Alfred Paris ,  Georg Bauer

IPC: C12M3/06 ,  B01D61/18 ,  B01D63/08 ,  B01D67/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M1/42 ,  C12M3/00

Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

公开(公告)号：US20130334120A1

公开(公告)日：2013-12-19

申请号：US13918193

申请日：2013-06-14

Applicant: President And Fellows Of Harvard College

Inventor： Donald E. Ingber ,  Joo Hun Kang ,  Richard C. Terry ,  Michael Super ,  Ryan M. Cooper ,  Karel Domansky ,  Chong Wing Yung

IPC: B03C1/28

CPC classification number: B03C1/28 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26

Abstract: A system and method for removing a target species from a fluid source is provided. The system includes a reciprocating fluid cleansing device, including a processing chamber with a port at a first end for fluid passage and a movable plunger at a second end, wherein the plunger in contact with a fluid includes a motorized mixing element for mixing the fluid with species-targeting magnetic particles. Motion of the plunger in a first direction transfers a first volume of the fluid from the fluid source into the processing chamber. Motion of the plunger in a second direction transfers the first volume of the fluid from the processing chamber to a fluid destination. At least one magnetic element provides a magnetic field gradient within the processing chamber. A connector connects the port of the first processing chamber to the fluid source and the fluid destination.

Abstract translation: 提供了用于从流体源中去除目标物种的系统和方法。 该系统包括往复式流体清洁装置，其包括处理室，其具有用于流体通道的第一端的端口和在第二端处的可移动柱塞，其中与流体接触的柱塞包括用于将流体与 以物种为目标的磁性颗粒。 柱塞在第一方向上的运动将第一体积的流体从流体源传送到处理室。 柱塞在第二方向的运动将第一体积的流体从处理室传送到流体目的地。 至少一个磁性元件在处理室内提供磁场梯度。 连接器将第一处理室的端口连接到流体源和流体目的地。