摘要:
Cloning and expression of DNA segments encoding bovine GM-CSF, and processes for producing recombinant bovine GM-CSF as a product of recombinant cell culture, are disclosed.
摘要:
A process for preparing bovine interleukin-2 (bIL-2) by culturing a microbial host transformed by a vector containing a DNA sequence encoding bIL-2 under conditions suitable for the expression of bIL-2 and recovering bIL-2.
摘要:
A chemically-synthesized oligonucleotide composing a portion of the nucleotide sequence of the human IL-2 is employed as a probe to isolate the gene coding for human IL-2. The human IL-2 gene is selected from a cDNA library prepared from RNA produced by mitogen-stimulated Jurkat cells. Double-stranded cDNA is prepared from polyadenylated RNA extracted from bovine cells thought to produce interleukin-2. Such cDNA is inserted within a plasmid vector and the recombinant plasmid employed to transform hosts. Plasmid DNA, prepared from pools of the transformed hosts, is hybridized with a probe composed of a large portion of the coding sequence of the human IL-2 gene. Pools of host cells that provide signal to the human cDNA probe are identified, subdivided, and rescreened until a single positive colony is identified. Bovine plasmid cDNA is prepared from this colony, and the bIL-2 gene is sequenced. The plasmid DNA is employed to express recombinant bovine IL-2 in yeast and bacterial expression systems, with the expressed bovine IL-2 purified to homogeneity by one or more reverse phase high-performance liquid chromatography procedures.
摘要:
A ride system is provided that allows selective relative positioning of vehicles in an amusement or theme park ride to simulate racing or other effects. The ride system includes a chassis that is adapted to be supported by and to travel on or along a length of track of a particular ride. A support is attached to the chassis and moves with the chassis during operation of the ride. The ride system includes first and second passenger vehicles that are spaced apart on and supported by the support. A drive assembly is linked to the support and configured to rotate the support about its central axis. During support rotation, the first and second vehicles are moved concurrently relative to the track to alter their relative positioning. The vehicles are each rotated about an axis that extends parallel to the rotation axis, and the rotation may be independent or concurrent.
摘要:
A round ride with passenger-initiated motion profiles controlling vertical vehicle movements. The ride includes a drive assembly with a rotating hub that includes support arms extending outward from the structure supporting passenger vehicles. Each of the vehicles includes a triggering device capable of generating either an onboard or offboard discrete signal when operated by a passenger causing a controller to select a motion profile from a number of stored profiles that each defines an actuator motion profile (or angular movement of a support arm about its pivotal mounting point to the rotatable central hub). The motion profiles define different angular rotations for a support arm to cause a vehicle to jump to differing heights, and the profile may be chosen by the controller based on vehicle height and position along the circular path and other environmental inputs such as a game state for the vehicle or a show/ride status.
摘要:
A round ride with passenger-initiated motion profiles controlling vertical vehicle movements. The ride includes a drive assembly with a rotating hub that includes support arms extending outward from the structure supporting passenger vehicles. Each of the vehicles includes a triggering device capable of generating either an onboard or offboard discrete signal when operated by a passenger causing a controller to select a motion profile from a number of stored profiles that each defines an actuator motion profile (or angular movement of a support arm about its pivotal mounting point to the rotatable central hub). The motion profiles define different angular rotations for a support arm to cause a vehicle to jump to differing heights, and the profile may be chosen by the controller based on vehicle height and position along the circular path and other environmental inputs such as a game state for the vehicle or a show/ride status.
摘要:
A defined serum-free medium that is capable of growing a wide range of suspension and monolayer cells includes a serum substitute composed of fetuin, transferrin, phosphatidylcholine, e.g., 1-oleoyl-2-palmitoyl-phosphatidylcholine, linoleic acid and cholesterol. The medium also includes various inorganic salts, carbohydrates, amino acids, buffering agents, vitamins, and compounds to simulate the natural cell environment.
摘要:
A ride system is provided that allows selective relative positioning of vehicles in an amusement or theme park ride to simulate racing or other effects. The ride system includes a chassis that is adapted to be supported by and to travel on or along a length of track of a particular ride. A support is attached to the chassis and moves with the chassis during operation of the ride. The ride system includes first and second passenger vehicles that are spaced apart on and supported by the support. A drive assembly is linked to the support and configured to rotate the support about its central axis. During support rotation, the first and second vehicles are moved concurrently relative to the track to alter their relative positioning. The vehicles are each rotated about an axis that extends parallel to the rotation axis, and the rotation may be independent or concurrent.
摘要:
A ride system is provided that allows selective relative positioning of vehicles in an amusement or theme park ride to simulate racing or other effects. The ride system includes a chassis that is adapted to be supported by and to travel on or along a length of track of a particular ride. A support is attached to the chassis and moves with the chassis during operation of the ride. The ride system includes first and second passenger vehicles that are spaced apart on and supported by the support. A drive assembly is linked to the support and configured to rotate the support about its central axis. During support rotation, the first and second vehicles are moved concurrently relative to the track to alter their relative positioning. The vehicles are each rotated about an axis that extends parallel to the rotation axis, and the rotation may be independent or concurrent.
摘要:
A ride system is provided that allows selective relative positioning of vehicles in an amusement or theme park ride to simulate racing or other effects. The ride system includes a chassis that is adapted to be supported by and to travel on or along a length of track of a particular ride. A support is attached to the chassis and moves with the chassis during operation of the ride. The ride system includes first and second passenger vehicles that are spaced apart on and supported by the support. A drive assembly is linked to the support and configured to rotate the support about its central axis. During support rotation, the first and second vehicles are moved concurrently relative to the track to alter their relative positioning. The vehicles are each rotated about an axis that extends parallel to the rotation axis, and the rotation may be independent or concurrent.