Reagent, chaotropic agent, and reagent kit for and applications of isolating nucleic acid by use of magnetic cellulose material
    1.
    发明授权
    Reagent, chaotropic agent, and reagent kit for and applications of isolating nucleic acid by use of magnetic cellulose material 有权
    试剂,离液剂和试剂盒,用于通过使用磁性纤维素材料分离核酸

    公开(公告)号:US08513405B2

    公开(公告)日:2013-08-20

    申请号:US12935681

    申请日:2008-04-02

    IPC分类号: C07H21/02 C07H21/00

    摘要: Disclosed are a chaotropic agent; a reagent including a chaotropic agent and a lithium salt; a reagent kit including a chaotropic agent; a chaotropic agent, a reagent, a reagent kit, and a method for isolating a nucleic acid by use of a magnetic cellulose material; a method for binding a nucleic acid to a magnetic cellulose material; a method for isolating a nucleic acid; and a method for purifying a chromosome DNA. It is required that each of the chaotropic agents, the reagents, and the reagent kits works with at least one solid-phase, magnetic cellulose-containing carrier to isolate a nucleic acid from non-nucleic acid substances. In addition, each chaotropic agent includes an alcohol substance and a substrate solution for adjusting the alcohol substance to an appropriate concentration and thereby promoting binding of the nucleic acid in a sample to the magnetic cellulose.

    摘要翻译: 公开了一种离液剂; 包含离液剂和锂盐的试剂; 包含离液剂的试剂盒; 离液剂,试剂,试剂盒和通过使用磁性纤维素材料分离核酸的方法; 将核酸与磁性纤维素材料结合的方法; 分离核酸的方法; 以及纯化染色体DNA的方法。 需要每种离液剂,试剂和试剂盒与至少一种固相含磁性纤维素的载体一起工作,以将核酸与非核酸物质分离。 此外,各离液剂包括醇物质和用于将醇物质调节至适当浓度的底物溶液,从而促进样品中的核酸与磁性纤维素的结合。

    REAGENT, CHAOTROPIC AGENT, AND REAGENT KIT FOR AND APPLICATIONS OF ISOLATING NUCLEIC ACID BY USE OF MAGNETIC CELLULOSE MATERIAL
    2.
    发明申请
    REAGENT, CHAOTROPIC AGENT, AND REAGENT KIT FOR AND APPLICATIONS OF ISOLATING NUCLEIC ACID BY USE OF MAGNETIC CELLULOSE MATERIAL 有权
    通过使用磁性纤维素材料分离核酸的试剂,化学试剂和试剂盒和应用

    公开(公告)号:US20110166338A1

    公开(公告)日:2011-07-07

    申请号:US12935681

    申请日:2008-04-02

    IPC分类号: C07H1/06 C07H1/08 H01F1/42

    摘要: Disclosed are a chaotropic agent; a reagent including a chaotropic agent and a lithium salt; a reagent kit including a chaotropic agent; a chaotropic agent, a reagent, a reagent kit, and a method for isolating a nucleic acid by use of a magnetic cellulose material; a method for binding a nucleic acid to a magnetic cellulose material; a method for isolating a nucleic acid; and a method for purifying a chromosome DNA. It is required that each of the chaotropic agents, the reagents, and the reagent kits works with at least one solid-phase, magnetic cellulose-containing carrier to isolate a nucleic acid from non-nucleic acid substances. In addition, each chaotropic agent includes an alcohol substance and a substrate solution for adjusting the alcohol substance to an appropriate concentration and thereby promoting binding of the nucleic acid in a sample to the magnetic cellulose.

    摘要翻译: 公开了一种离液剂; 包含离液剂和锂盐的试剂; 包含离液剂的试剂盒; 离液剂,试剂,试剂盒和通过使用磁性纤维素材料分离核酸的方法; 将核酸与磁性纤维素材料结合的方法; 分离核酸的方法; 以及纯化染色体DNA的方法。 需要每种离液剂,试剂和试剂盒与至少一种固相含磁性纤维素的载体一起工作,以将核酸与非核酸物质分离。 此外,各离液剂包括醇物质和用于将醇物质调节至适当浓度的底物溶液,从而促进样品中的核酸与磁性纤维素的结合。

    METHOD, AGENT, AND KIT FOR ISOLATING NUCLEIC ACIDS
    3.
    发明申请
    METHOD, AGENT, AND KIT FOR ISOLATING NUCLEIC ACIDS 有权
    方法,用于分离核酸的试剂和试剂盒

    公开(公告)号:US20110097782A1

    公开(公告)日:2011-04-28

    申请号:US12905777

    申请日:2010-10-15

    CPC分类号: C12N15/1013 C12N15/1006

    摘要: A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.

    摘要翻译: 提供了分离核酸的方法。 该方法包括提供含有至少一种核酸的生物样品,并在合适的条件下将生物样品与分离剂混合,以在单步骤中分离生物样品中的核酸,其中所述分离剂含有1-40重量% 的PEG和/或大于30重量%的低分子量醇,盐和洗涤剂。 分离的核酸通过核酸溶解度的变化与固体支持物结合。 另外,本发明还提供了用于分离核酸的分离剂和试剂盒。

    Method for isolating nucleic acids
    4.
    发明授权
    Method for isolating nucleic acids 有权
    分离核酸的方法

    公开(公告)号:US09006419B2

    公开(公告)日:2015-04-14

    申请号:US12905777

    申请日:2010-10-15

    IPC分类号: C12N1/08 C12N15/10

    CPC分类号: C12N15/1013 C12N15/1006

    摘要: A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.

    摘要翻译: 提供了分离核酸的方法。 该方法包括提供含有至少一种核酸的生物样品,并在合适的条件下将生物样品与分离剂混合,以在单步骤中分离生物样品中的核酸,其中所述分离剂含有1-40重量% 的PEG和/或大于30重量%的低分子量醇,盐和洗涤剂。 分离的核酸通过核酸溶解度的变化与固体支持物结合。 另外,本发明还提供了用于分离核酸的分离剂和试剂盒。

    PEPTIDE COMPOUNDS
    5.
    发明申请
    PEPTIDE COMPOUNDS 有权
    肽类化合物

    公开(公告)号:US20080114152A1

    公开(公告)日:2008-05-15

    申请号:US11681094

    申请日:2007-03-01

    IPC分类号: C07K7/00

    摘要: A peptide compound for measuring sulfur compound and ammonia and measurement method and device using the compound. The method comprises contacting an analyte with a sensory device coated with the peptide compound to produce a signal, processing the signal to produce a result, and comparing the result with a database to define the presence of the sulfur compound and ammonia in the analyte.

    摘要翻译: 用于测量硫化合物和氨的肽化合物和使用该化合物的测量方法和装置。 该方法包括使分析物与涂覆有肽化合物的感觉装置接触以产生信号,处理信号以产生结果,并将结果与​​数据库进行比较以定义分析物中硫化合物和氨的存在。

    Magnetic separation device
    9.
    发明授权
    Magnetic separation device 失效
    磁选机

    公开(公告)号:US07740759B2

    公开(公告)日:2010-06-22

    申请号:US11736555

    申请日:2007-04-17

    IPC分类号: B01D35/06 B03C1/02 B01L9/06

    摘要: A magnetic separation device separating magnetic material from a working fluid of a container is disclosed. The magnetic separation device includes a first element, a second element and a magnetic assembly structure including a plurality of magnetic units. The first element includes a first body, a plurality of first main positioning portions disposed on the first body, and a plurality of first sub-positioning portions disposed on the first body and next to the first main positioning portions to receive the container. The second element includes a second body, a plurality of second main positioning portions disposed on the second body, and a plurality of second sub-positioning portions disposed on the second body and next to the second main positioning portions to receive the container. The magnetic units disposed on the first main positioning portions and the second main positioning portions absorb magnetic material from the working fluid.

    摘要翻译: 公开了一种将磁性材料与容器的工作流体分离的磁选机。 磁分离装置包括第一元件,第二元件和包括多个磁性单元的磁性组件结构。 第一元件包括第一主体,设置在第一主体上的多个第一主定位部以及设置在第一主体上并靠近第一主定位部分以接收容器的多个第一副定位部。 第二元件包括第二主体,设置在第二主体上的多个第二主定位部分和设置在第二主体上并与第二主定位部分相邻以接收容器的多个第二副定位部分。 设置在第一主定位部分上的磁性单元和第二主定位部分吸收来自工作流体的磁性材料。

    MAGNETIC SEPARATION DEVICE
    10.
    发明申请
    MAGNETIC SEPARATION DEVICE 失效
    磁选分离装置

    公开(公告)号:US20080156714A1

    公开(公告)日:2008-07-03

    申请号:US11736555

    申请日:2007-04-17

    IPC分类号: B01D35/06

    摘要: A magnetic separation device separating magnetic material from a working fluid of a container is disclosed. The magnetic separation device includes a first element, a second element and a magnetic assembly structure including a plurality of magnetic units. The first element includes a first body, a plurality of first main positioning portions disposed on the first body, and a plurality of first sub-positioning portions disposed on the first body and next to the first main positioning portions to receive the container. The second element includes a second body, a plurality of second main positioning portions disposed on the second body, and a plurality of second sub-positioning portions disposed on the second body and next to the second main positioning portions to receive the container. The magnetic units disposed on the first main positioning portions and the second main positioning portions absorb magnetic material from the working fluid.

    摘要翻译: 公开了一种将磁性材料与容器的工作流体分离的磁选机。 磁分离装置包括第一元件,第二元件和包括多个磁性单元的磁性组件结构。 第一元件包括第一主体,设置在第一主体上的多个第一主定位部以及设置在第一主体上并靠近第一主定位部分以接收容器的多个第一副定位部。 第二元件包括第二主体,设置在第二主体上的多个第二主定位部分和设置在第二主体上并与第二主定位部分相邻以接收容器的多个第二副定位部分。 设置在第一主定位部分上的磁性单元和第二主定位部分吸收来自工作流体的磁性材料。