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    Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector
    1.
    发明授权
    Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector 失效
    通过载体对转染植物进行细胞质基因抑制或基因表达

    公开(公告)号：US07498480B2

    公开(公告)日：2009-03-03

    申请号：US10634221

    申请日：2003-08-04

    申请人： Peter D. Roberts ,  Monto H. Kumagai ,  Andrew A. Vaewhongs

    发明人： Peter D. Roberts ,  Monto H. Kumagai ,  Andrew A. Vaewhongs

    IPC分类号： C12N15/83 ,  C12N5/04 ,  C12N15/82

    CPC分类号： A01H1/04 ,  A01H5/12 ,  C12N15/1034 ,  C12N15/8261 ,  C12Q1/68 ,  Y02A40/146

    摘要： This invention is directed to a monopartite RNA viral vector comprising modified tobravirus RNA-1 comprising an inserted foreign RNA sequence. This invention is also directed to a bipartite RNA viral vector derived from a tobravirus, wherein the vector comprises one or more foreign RNA sequences. The invention is directed to a method of silencing one or more endogenous plant host genes and a method of simultaneously silencing a plant host gene and expressing a foreign gene in a plant host. Such methods comprise infecting a plant host with a bipartite vector comprising modified tobravirus RNA-1 and RNA-2. The invention is further directed to a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant host, and a method of determining the presence of a trait in a plant host, using a monopartite or bipartite viral vector derived from a tobravirus.

    摘要翻译： 本发明涉及包含经插入的外源RNA序列的修饰的载体RNA-1的单分子RNA病毒载体。 本发明还涉及衍生自托巴威病毒的二分之一RNA病毒载体，其中所述载体包含一个或多个外源RNA序列。 本发明涉及沉默一种或多种内源性植物宿主基因的方法和同时使植物宿主基因沉默并在植物宿主中表达外源基因的方法。 这样的方法包括用包含修饰的tobravirus RNA-1和RNA-2的二重载体感染植物宿主。 本发明进一步涉及一种编码植物功能基因谱的方法，改变植物宿主的表型或生物化学的方法，以及使用单一或二分之一病毒测定植物宿主中性状的存在的方法 衍生自Tobravirus的载体。

    Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector
    2.
    发明授权
    Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector 失效
    通过托博韦病毒载体在转染植物中细胞质抑制基因表达

    公开(公告)号：US06700040B2

    公开(公告)日：2004-03-02

    申请号：US09771035

    申请日：2001-01-25

    申请人： Peter D. Roberts ,  Andy A. Vaewhongs ,  Monto H. Kumagai

    发明人： Peter D. Roberts ,  Andy A. Vaewhongs ,  Monto H. Kumagai

    IPC分类号： C12N1583

    CPC分类号： A01H1/04 ,  A01H5/12 ,  C12N15/1034 ,  C12N15/8261 ,  C12Q1/68 ,  Y02A40/146

    摘要： This invention is directed to a monopartite RNA viral vector comprising modified tobravirus RNA-1 comprising an inserted foreign RNA sequence. This invention is also directed to a bipartite RNA viral vector derived from a tobravirus, wherein the vector comprises one or more foreign RNA sequences. The invention is directed to a method of silencing one or more endogenous plant host genes and a method of simultaneously silencing a plant host gene and expressing a foreign gene in a plant host. Such methods comprise infecting a plant host with a bipartite vector comprising modified tobravirus RNA-1 and RNA-2.

    摘要翻译： 本发明涉及包含经插入的外源RNA序列的修饰的载体RNA-1的单分子RNA病毒载体。 本发明还涉及衍生自托巴威病毒的二分之一RNA病毒载体，其中所述载体包含一个或多个外源RNA序列。 本发明涉及沉默一种或多种内源性植物宿主基因的方法和同时使植物宿主基因沉默并在植物宿主中表达外源基因的方法。 这样的方法包括用包含修饰的tobravirus RNA-1和RNA-2的二重载体感染植物宿主。

    Method for conferring herbicide, pest, or disease resistance in plant hosts
    4.
    发明授权
    Method for conferring herbicide, pest, or disease resistance in plant hosts 失效
    在植物宿主中赋予除草剂，有害生物或抗病性的方法

    公开(公告)号：US06303848B1

    公开(公告)日：2001-10-16

    申请号：US09359302

    申请日：1999-07-21

    申请人： Monto H. Kumagai ,  Guy R. della-Cioppa

    发明人： Monto H. Kumagai ,  Guy R. della-Cioppa

    IPC分类号： A01H500

    CPC分类号： C12N15/8283 ,  A01H1/04 ,  A01H5/12 ,  C12N9/0004 ,  C12N9/1092 ,  C12N15/1034 ,  C12N15/1079 ,  C12N15/8274 ,  C12Q1/68

    摘要： The present invention relates to a method for conferring herbicide, pest, and disease resistance in plant hosts. Specifically, the present invention employs transient viral expression vectors to express proteins or enzymes conferring resistance in plant hosts. In addition, a library of nucleotide sequence variants in a sense or antisene orientation may be used to determine the targets of an herbicide or pathogen and to screen suitable viral nucleic acids for herbicide, pest, and disease resistance.

    摘要翻译： 本发明涉及在植物宿主中赋予除草剂，害虫和抗病性的方法。 具体地，本发明使用瞬时病毒表达载体来表达赋予植物宿主抗性的蛋白质或酶。 此外，可以使用有意义或抗血清取向的核苷酸序列变体文库来确定除草剂或病原体的靶标，并筛选合适的病毒核酸用于除草剂，害虫和抗病性。

    Pichia pastoris alcohol oxidase ZZA1 and ZZA2 regulatory regions for heterologous gene expression
    5.
    发明授权
    Pichia pastoris alcohol oxidase ZZA1 and ZZA2 regulatory regions for heterologous gene expression 失效
    巴斯德毕赤酵母醇氧化酶ZZA1和ZZA2调节区用于异源基因表达

    公开(公告)号：US5641661A

    公开(公告)日：1997-06-24

    申请号：US220606

    申请日：1994-03-25

    申请人： Monto H. Kumagai ,  Genadie G. Sverlow

    发明人： Monto H. Kumagai ,  Genadie G. Sverlow

    IPC分类号： C12N9/04 ,  C12N9/32 ,  C12N15/81 ,  C12P7/10 ,  C12P7/06 ,  C12N1/16 ,  C12N1/19 ,  C12N15/00

    CPC分类号： C12N9/2422 ,  C12N15/815 ,  C12N9/0006 ,  C12P7/10 ,  Y02E50/16

    摘要： Two novel genomic clones, ZZA1 and ZZA2, were isolated which encode for Pichia pastoris alcohol oxidase isozymes. The 5' non-coding region of ZZA1 contains common structural features involved in the transcription and translation of eukaryotic genes. Comparison of the nucleotide sequences of the ZZA1 and AOX1 51 noncoding regions showed that they are 66% similar to each other.The rice .alpha.-amylase gene OS103 was placed under the transcriptional control of the ZZA1 promoter. The nucleotide sequences of ZZA1 and other methanol-regulated promoters were analyzed. A highly conserved sequence (TTGNNNGCTTCCAANNNNNTGGT) (SEQ ID NO:2) was found in the 51 flanking region. A yeast strain containing the ZZA1 -OS103 fusion and secreting biologically active a-amylase into the culture media while converting starch to ethanol was produced. The ZZA1 and ZZA2 regulatory sequences may be used to contol the expression of other heterologous proteins in multiple yeast species.Methods of purifying proteins that are regulators of alcohol oxidase expression (referred to as AOER proteins) and methods of isolating these proteins are also provided.The invention also provides for the efficient conversion of starch into ethanol.

    摘要翻译： 分离出两个新的基因组克隆ZZA1和ZZA2，其编码巴斯德毕赤酵母醇氧化酶同功酶。 ZZA1的5'非编码区包含涉及真核基因转录和翻译的常见结构特征。 ZZA1和AOX1 51非编码区核苷酸序列的比较表明，它们的相似度为66％。 将水稻α-淀粉酶基因OS103置于ZZA1启动子的转录控制下。 分析了ZZA1和其他甲醇调节启动子的核苷酸序列。 在51个侧翼区域发现了高度保守的序列（TTGNNNGCTTCCAANNNNNTGGT）（SEQ ID NO：2）。 产生含有ZZA1-OS103融合并在将淀粉转化为乙醇时将培养基分泌到生物活性α-淀粉酶中的酵母菌株。 ZZA1和ZZA2调节序列可用于调节多种酵母菌种中其他异源蛋白质的表达。 还提供了纯化作为醇氧化酶表达调节剂（称为AOER蛋白）的蛋白质的纯化方法和分离这些蛋白质的方法。 本发明还提供了将淀粉有效转化成乙醇。

    Method to personalize and enhance the value of consumer items for use in outdoor activities
    6.
    发明授权
    Method to personalize and enhance the value of consumer items for use in outdoor activities 有权
    个性化，提高消费品价值的方法，用于户外活动

    公开(公告)号：US07598869B2

    公开(公告)日：2009-10-06

    申请号：US11560863

    申请日：2006-11-17

    申请人： Monto H. Kumagai ,  Philip Miller

    发明人： Monto H. Kumagai ,  Philip Miller

    IPC分类号： G08B13/14

    CPC分类号： G07G1/009 ,  G06Q30/02

    摘要： The present invention provides a method to personalize and enhance the value of consumer items for use in outdoor activities. It utilizes radio frequency identification (RFID) tags, computers, PDAs, and cell phones to link, record, and retrieve positional, personal, and historical data. The personalization and association of an object to a unique event by the consumer will lead to enhanced value of the product. Information that is stored on cell phones, PDAs, computers, and external databases are transferred as multimedia messages. The information is cataloged and organized using the unique RFID number. The subject line of the multimedia message is the RFID tag number. Commercial items such as skis, coats, hats, shoes, shirts, pants, gloves, sun glasses, surfboards, bicycles, wetsuits, cell phones, computers, golf clubs, golf balls, fishing poles, and lures will be produced that contain RFID tags.

    摘要翻译： 本发明提供一种个性化和增强用于户外活动的消费品的价值的方法。 它利用射频识别（RFID）标签，计算机，PDA和手机链接，记录和检索位置，个人和历史数据。 消费者将个人化和个性化与独特事件的关联将导致产品的价值增加。 存储在手机，PDA，计算机和外部数据库上的信息作为多媒体消息传送。 信息使用唯一的RFID编号进行编目和组织。 多媒体消息的主题行是RFID标签号。 将生产包含RFID标签的商品，如滑雪板，大衣，帽子，鞋子，衬衫，裤子，手套，太阳眼镜，冲浪板，自行车，潜水服，手机，电脑，高尔夫球杆，高尔夫球，钓鱼竿和诱饵 。

    Method to personalize real estate brochures, postcards, books, and photo documents using radio frequency identification tags
    9.
    发明申请
    Method to personalize real estate brochures, postcards, books, and photo documents using radio frequency identification tags 审中-公开
    使用射频识别标签个性化房地产手册，明信片，书籍和照片文件的方法

    公开(公告)号：US20100019482A1

    公开(公告)日：2010-01-28

    申请号：US12573880

    申请日：2009-10-05

    申请人： Monto H. Kumagai ,  Philip Miller

    发明人： Monto H. Kumagai ,  Philip Miller

    IPC分类号： B42D15/00 ,  H04Q5/22

    CPC分类号： G07G1/009 ,  G06Q30/02 ,  G06Q50/16 ,  G07F17/26

    摘要： The present invention provides a method to personalize and enhance photo documents. It utilizes radio frequency identification (RFID) tags, computers, PDAs, and cell phones to link, record, and retrieve positional, personal, and historical data. The personalization and association of a photo document to a unique event by the consumer will lead to an enhanced value of the product. Photo documents containing RFID tags are associated with personal images, movies, text, and audio files. Information that is stored on cell phones, PDAs, computers, and external databases is transferred and shared using cellular networks, email, text, and multimedia messaging. This invention describes a system to record, retrieve, and share personal experiences and event information in association with commercial products such as real estate properties.

    摘要翻译： 本发明提供个性化和增强照片文件的方法。 它利用射频识别（RFID）标签，计算机，PDA和手机链接，记录和检索位置，个人和历史数据。 照片文件与消费者的独特事件的个性化和关联将导致产品的增值。 包含RFID标签的照片文件与个人图像，电影，文本和音频文件相关联。 存储在手机，PDA，计算机和外部数据库上的信息使用蜂窝网络，电子邮件，文本和多媒体消息传送和共享。 本发明描述了一种与诸如房地产属性的商业产品相关联的记录，检索和分享个人体验和事件信息的系统。

    Method to personalize, promote, and distribute multimedia content using radio frequency identification
    10.
    发明申请
    Method to personalize, promote, and distribute multimedia content using radio frequency identification 审中-公开
    使用射频识别来个性化，推广和分发多媒体内容的方法

    公开(公告)号：US20090322493A1

    公开(公告)日：2009-12-31

    申请号：US12553282

    申请日：2009-09-03

    申请人： Monto H. Kumagai ,  Philip Miller

    发明人： Monto H. Kumagai ,  Philip Miller

    IPC分类号： H04Q5/22

    CPC分类号： G06Q30/02

    摘要： The present invention provides a method to advertise real estate, events, and consumer items. It utilizes radio frequency identification (RFID) tags, computers, PDAs, and cell phones to link, record, and retrieve positional, personal, and historical data. The personalization and association of an object to a unique event by the consumer will lead to enhanced value of a product. Information that is stored on cell phones, PDAs, computers, and external databases are transferred as multimedia messages. The information is cataloged and organized using the unique RFID number. The subject line of the multimedia message is the RFID tag number. The system can be used to advertise real estate, recreational events, and commercial items such as skis, coats, hats, shoes, shirts, pants, gloves, sun glasses, surfboards, bicycles, wetsuits, cell phones, computers, trading cards, golf clubs, golf balls, fishing poles, and lures.

    摘要翻译： 本发明提供一种广告房地产，事件和消费品的方法。 它利用射频识别（RFID）标签，计算机，PDA和手机链接，记录和检索位置，个人和历史数据。 消费者将对象与独特事件的个性化和关联将导致产品的价值增加。 存储在手机，PDA，计算机和外部数据库上的信息作为多媒体消息传送。 信息使用唯一的RFID编号进行编目和组织。 多媒体消息的主题行是RFID标签号。 该系统可用于宣传房地产，娱乐活动和商业项目，如滑雪板，大衣，帽子，鞋子，衬衫，裤子，手套，太阳眼镜，冲浪板，自行车，潜水衣，手机，电脑，交易卡，高尔夫 俱乐部，高尔夫球，钓鱼竿和诱饵。