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    Multiple Gene Expression Including sORF Constructs and Methods with Polyproteins, Pro-Proteins and Proteolysis
    8.
    发明申请
    Multiple Gene Expression Including sORF Constructs and Methods with Polyproteins, Pro-Proteins and Proteolysis 审中-公开
    包括多聚蛋白，前蛋白和蛋白水解的sORF构建体和方法的多基因表达

    公开(公告)号：US20130243789A1

    公开(公告)日：2013-09-19

    申请号：US13739304

    申请日：2013-01-11

    申请人： Gerald R. Carson ,  Jochen G. Salfeld ,  Dean A. Regier ,  Jijie Gu ,  Wendy Gion ,  Yune Z. Kunes

    发明人： Gerald R. Carson ,  Jochen G. Salfeld ,  Dean A. Regier ,  Jijie Gu ,  Wendy Gion ,  Yune Z. Kunes

    IPC分类号： C12N15/67

    CPC分类号： C12N15/67 ,  C07K16/00 ,  C07K2319/50 ,  C07K2319/92 ,  C12N15/1055 ,  C12P21/02 ,  C12P21/06

    摘要： Disclosed are useful constructs and methods for the expression of proteins using primary translation products that are processed within a recombinant host cell. Constructs comprising a single open reading frame (sORF) are described for protein expression including expression of multiple polypeptides. A primary translation product (a pro-protein or a polyprotein) contains polypeptides such as inteins or hedgehog family auto-processing domains, or variants thereof, inserted in frame between multiple protein subunits of interest. Also disclosed are independent aspects of conducting efficient expression, secretion, and/or multimeric assembly of proteins such as immunoglobulins.

    摘要翻译： 公开了使用在重组宿主细胞内处理的主要翻译产物来表达蛋白质的有用构建体和方法。 描述了包含单个开放阅读框（sORF）的构建体用于蛋白质表达，包括多个多肽的表达。 初级翻译产物（前蛋白质或多蛋白质）含有多肽，例如内含肽或刺猬蛋白家族自身处理结构域或其变体，其插入到感兴趣的多个蛋白质亚基之间。 还公开了进行蛋白质例如免疫球蛋白的有效表达，分泌和/或多聚体装配的独立方面。