公开(公告)号：US06399294B1

公开(公告)日：2002-06-04

申请号：US08817441

申请日：1997-07-11

申请人： Pierre Charneau ,  François Clavel ,  Andrew Borman ,  Caroline Quillent ,  Denise Guetard ,  Luc Montagnier ,  Jacqueline Donjon De Saint-Martin ,  Jacques Cohen

发明人： Pierre Charneau ,  François Clavel ,  Andrew Borman ,  Caroline Quillent ,  Denise Guetard ,  Luc Montagnier ,  Jacqueline Donjon De Saint-Martin ,  Jacques Cohen

IPC分类号： C12Q170

CPC分类号： C07K14/005 ,  C12N2740/16021 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  G01N33/56988 ,  Y10S435/975

摘要： An HIV-1 type (or subtype) O retrovirus protein, or a natural or synthetic polypeptide or peptide including at least a part of said protein, which is capable of being recognised by antibodies isolated from a serum resulting from infection by an HIV-1 type O VAU strain or an HIV-1 type (or subtype) O DUR strain.

摘要翻译： HIV-1型（或亚型）O逆转录病毒蛋白，或包含至少一部分所述蛋白质的天然或合成多肽或肽，其能够被从HIV-1感染引起的血清分离的抗体识别 O型VAU菌株或HIV-1型（或亚型）O DUR菌株。

公开(公告)号：US08236324B2

公开(公告)日：2012-08-07

申请号：US11591678

申请日：2006-11-02

申请人： Pierre Charneau ,  François Clavel ,  Andrew Borman ,  Caroline Quillent ,  Denise Guetard ,  Luc Montagnier ,  Jacqueline Donjon de Saint-Martin ,  Jacques Cohen

发明人： Pierre Charneau ,  François Clavel ,  Andrew Borman ,  Caroline Quillent ,  Denise Guetard ,  Luc Montagnier ,  Jacqueline Donjon de Saint-Martin ,  Jacques Cohen

IPC分类号： A61K39/21

CPC分类号： C07K14/005 ,  C12N2740/16021 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  G01N33/56988 ,  Y10S435/975

摘要： An HIV-1 type (or subtype) O retrovirus protein, or a natural or synthetic polypeptide or peptide including at least a part of said protein, which is capable of being recognized by antibodies isolated from a serum resulting from infection by an HIV-1 type O VAU strain or an HIV-1 type (or subtype) O DUR strain.

摘要翻译： HIV-1型（或亚型）O逆转录病毒蛋白，或包含至少一部分所述蛋白质的天然或合成多肽或肽，其能够被从HIV-1感染引起的血清分离的抗体识别 O型VAU菌株或HIV-1型（或亚型）O DUR菌株。

公开(公告)号：US07157225B2

公开(公告)日：2007-01-02

申请号：US10026741

申请日：2001-12-27

申请人： Pierre Charneau ,  François Clavel ,  Andrew Borman ,  Caroline Quillent ,  Denise Guetard ,  Luc Montagnier ,  Jacqueline Donjon De Saint-Martin ,  Jacques H. M. Cohen

发明人： Pierre Charneau ,  François Clavel ,  Andrew Borman ,  Caroline Quillent ,  Denise Guetard ,  Luc Montagnier ,  Jacqueline Donjon De Saint-Martin ,  Jacques H. M. Cohen

IPC分类号： C12Q1/70 ,  A61K39/21 ,  A61K38/04 ,  A61K38/16 ,  C07H21/04

CPC分类号： C07K14/005 ,  C12N2740/16021 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  G01N33/56988 ,  Y10S435/975

摘要： An HIV-1 type (or subtype) O retrovirus protein, or a natural or synthetic polypeptide or peptide including at least a part of said protein, which is capable of being recognised by antibodies isolated from a serum resulting from infection by an HIV-1 type O VAU strain or an HIV-1 type (or subtype) O DUR strain.

摘要翻译： HIV-1型（或亚型）O逆转录病毒蛋白，或包含至少一部分所述蛋白质的天然或合成多肽或肽，其能够被从HIV-1感染引起的血清分离的抗体识别 O型VAU菌株或HIV-1型（或亚型）O DUR菌株。

公开(公告)号：US07341731B2

公开(公告)日：2008-03-11

申请号：US10180460

申请日：2002-06-27

申请人： Luc Montagnier ,  Solange Chamaret ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet ,  Marianne Rey ,  Christine Rouzioux ,  Christine Katlama

发明人： Luc Montagnier ,  Solange Chamaret ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet ,  Marianne Rey ,  Christine Rouzioux ,  Christine Katlama

IPC分类号： A61K39/21

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

摘要： The invention relates to a composition comprising at least one antigen, wherein the antigen is a protein or glycoprotein of a HIV-2 virus, wherein the HIV-2 virus has infectious properties with respect to human T4 lymphocytes and the essential morphological or immunological property of at least one of the retroviruses deposited at the CNCM under No. I-502, I-532, I-642, and I-643. It also relates to a process for the production of HIV-2 retrovirus antigens by lysing cells infected with the virus or lysing purified virus and recovering the antigens.

摘要翻译： 本发明涉及包含至少一种抗原的组合物，其中所述抗原是HIV-2病毒的蛋白质或糖蛋白，其中所述HIV-2病毒相对于人T4淋巴细胞具有感染性质，以及所述HIV-2病毒的基本形态学或免疫学性质 至少有一种在CNCM下存放在I-502，I-532，I-642和I-643下的逆转录病毒。 它还涉及通过裂解感染病毒的细胞或裂解纯化的病毒并回收抗原来生产HIV-2逆转录病毒抗原的方法。

公开(公告)号：US06265149B1

公开(公告)日：2001-07-24

申请号：US08470491

申请日：1995-06-06

申请人： Luc Montagnier ,  Solange Chamaret ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet ,  Marianne Rey ,  Christine Rouzioux ,  Christine Katlama

发明人： Luc Montagnier ,  Solange Chamaret ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet ,  Marianne Rey ,  Christine Rouzioux ,  Christine Katlama

IPC分类号： C12Q170

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

摘要： The claimed invention is directed toward in vitro methods for the detection of human immunodeficiency virus type 2 (HIV-2) antibodies employing polypeptide fragments of HIV-2 Gag.

摘要翻译： 所要求保护的发明涉及使用HIV-2Gag的多肽片段检测使用HIV-2抗体的人类免疫缺陷病毒的体外方法。

公开(公告)号：US07115363B1

公开(公告)日：2006-10-03

申请号：US08470489

申请日：1995-06-06

申请人： Luc Montagnier ,  Solange Chamaret ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Francoise Brun-Vezinet ,  Marianne Rey ,  Christine Rouzioux ,  Christine Katlama

发明人： Luc Montagnier ,  Solange Chamaret ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Francoise Brun-Vezinet ,  Marianne Rey ,  Christine Rouzioux ,  Christine Katlama

IPC分类号： C12Q1/70

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

摘要： The invention relates to a new class of retroviruses, designated by HIV-2, of which samples have been deposited to the ECACC under numbers 87.01.1001 and 87.01.1002 and to the NCIB under numbers 12.398 and 12.399.It relates also to antigens capable to be obtained from this virus, particularly proteins p12, p16, p26 and gp140. These various antigens can be used for the diagnosis of the disease, especially by contacting these antigens with a serum of a patient submitted to the diagnosis.It relates to immunogenic compositions containing more particularly the glycoprotein gp140. Finally it concerns nucleotidic sequences, which can be used especially as hybridization probes, derived from the RNA of HIV-2.

摘要翻译： 本发明涉及由HIV-2指定的一类新的逆转录病毒，其中样品已经按照号码87.01.1001和87.01.1002保藏在ECACC，编号为12.398和12.399的NCIB。 它还涉及能够从该病毒获得的抗原，特别是蛋白质p12，p16，p26和gp140。 这些各种抗原可以用于疾病的诊断，特别是通过将这些抗原与患者的血清接触进行诊断。 本发明涉及更具体地包含糖蛋白gp140的免疫原性组合物。 最后，它涉及核苷酸序列，其可以特别用作衍生自HIV-2的RNA的杂交探针。

公开(公告)号：US06664041B2

公开(公告)日：2003-12-16

申请号：US09862511

申请日：2001-05-23

申请人： Luc Montagnier ,  Solange Chamaret ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet ,  Marianne Rey ,  Christine Rouzioux ,  Christine Katlama

发明人： Luc Montagnier ,  Solange Chamaret ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet ,  Marianne Rey ,  Christine Rouzioux ,  Christine Katlama

IPC分类号： C12Q170

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

摘要： The invention relates to a new class of retroviruses, designated by HIV-2, of which samples have been deposited to the ECACC under numbers 87.01.1001 and 87.01.1002 and to the NCIB under numbers 12.398 and 12.399. It relates also to antigens capable to be obtained from this virus, particularly proteins p12, p16, p26 and gp140. These various antigens can be used for the diagnosis of the disease, especially by contacting these antigens with a serum of a patient submitted to the diagnosis. It relates to immunogenic compositions containing more particularly the glycoprotein gp140. Finally it concerns nucleotidic sequences, which can be used especially as hybridization probes, derived from the RNA of HIV-2.

摘要翻译： 本发明涉及由HIV-2指定的一类新的逆转录病毒，其中样品已经以数字87.01.1001和87.01.1002保藏在ECACC中，并且编号为12.398和12.399的NCIB。还涉及抗原能力 从该病毒获得，特别是蛋白质p12，p16，p26和gp140。 这些各种抗原可以用于疾病的诊断，特别是通过使这些抗原与患者的血清接触进行诊断。其涉及包含更特别是糖蛋白gp140的免疫原性组合物。 最后，它涉及核苷酸序列，其可以特别用作衍生自HIV-2的RNA的杂交探针。

公开(公告)号：US06429306B1

公开(公告)日：2002-08-06

申请号：US08392613

申请日：1995-02-22

申请人： Luc Montagnier ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet

发明人： Luc Montagnier ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet

IPC分类号： C07H2104

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

摘要： The present invention is directed toward a novel human retrovirus isolated from West African AIDS patients. This virus was originally designated lymphadenopathy associated virus (LAV) type II and subsequently renamed the human immunodeficiency virus type 2, or HIV-2. This virus is genotypically and phenotypically distinct from both human immunodeficiency virus type 1 (HIV-1) and the simian immunodeficiency virus (SIV). A recombinant &lgr; phage library was prepared by subjecting HIV-2-infected CEM genomic DNA to digestion with Sau3AI. The library was screened with an HIV-2-specific cDNA probe and molecular clones of the virus were obtained. Restriction maps and the nucleotide sequences of these clones were ascertained. These nucleic acids should prove useful, inter alia, as probes for the detection of HIV-2 in biological samples and for the expression of HIV-2 gene products.

摘要翻译： 本发明针对从西非艾滋病患者分离的新的人类逆转录病毒。 该病毒最初被命名为II型淋巴结病相关病毒（LAV），随后更名为2型人类免疫缺陷病毒或HIV-2。 这种病毒在人类免疫缺陷病毒1型（HIV-1）和猿猴免疫缺陷病毒（SIV）的基因型和表型上不同。 通过使感染了HIV-2感染的CEM基因组DNA用Sau3AI消化来制备重组羔羊噬菌体文库。 用HIV-2特异性cDNA探针筛选文库，获得病毒的分子克隆。 确定了这些克隆的限制图和核苷酸序列。 这些核酸尤其应用于检测生物样品中的HIV-2和HIV-2基因产物的表达。

公开(公告)号：US06558923B2

公开(公告)日：2003-05-06

申请号：US09988010

申请日：2001-11-16

申请人： Sylvie Paulous ,  Pierre Charneau ,  Véronique Zennou ,  François Clavel ,  Esther Race ,  Elisabeth Dam ,  Véronique Obry

发明人： Sylvie Paulous ,  Pierre Charneau ,  Véronique Zennou ,  François Clavel ,  Esther Race ,  Elisabeth Dam ,  Véronique Obry

IPC分类号： C12P2106

CPC分类号： C12Q1/703 ,  C12Q1/70

摘要： The invention provides an in vitro single cycle, recombinant virus assay (RVA) for determining inhibition of HIV replication by a protease inhibitor. The assay comprises transfecting a human epithelial cell line with amplified HIV protease sequences of an HIV virus; an HIV envelope defective molecular clone having a complete deletion of its protease coding sequence as well as two separate deletions in its env gene and a deletion of part of its gag gene; and a plasmid containing HIV envelope coding sequence under the control of a promoter for phenotypic complementation of the envelope defective molecular clone. The transfected cells are cultured in the presence of a protease inhibitor to produce a testable stock of infectious particles that can be used to infect indicator cells containing an indicator gene without amplification of the infectious particles prior to infecting the indicator cells. Accumulation of indicator gene product is a measure of inhibition of HIV replication by the protease inhibitor.

公开(公告)号：US06440657B1

公开(公告)日：2002-08-27

申请号：US09478492

申请日：2000-01-06

申请人： Luc Montangnier ,  Bernard Krust ,  Solange Chamaret ,  François Clavel ,  Jean-Claude Chermann ,  Françoise Barre-Sinoussi ,  Marc Alizon ,  Pierre Sonigo ,  Stewart Cole ,  Olivier Danos ,  Simon Wain-Hobson

发明人： Luc Montangnier ,  Bernard Krust ,  Solange Chamaret ,  François Clavel ,  Jean-Claude Chermann ,  Françoise Barre-Sinoussi ,  Marc Alizon ,  Pierre Sonigo ,  Stewart Cole ,  Olivier Danos ,  Simon Wain-Hobson

IPC分类号： C12Q170

CPC分类号： C07K14/005 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703

摘要： The molecular cloning and characterization of a novel human retrovirus, designated lymphadenopathy-associated virus, or LAV, is disclosed. LAV was originally isolated from a patient with acquired immune deficiency syndrome (AIDS). A cloned LAV complementary DNA (cDNA) was used to screen a library of recombinant phages constructed from the genomic DNA of LAV-infected T lymphocytes. The nucleotide sequence of an insert obtained from the recombinant phage clone &lgr;J19 was ascertained through M13 shotgun cloning and the dideoxy chain termination sequencing method. The env coding region was identified and various hydrophilic peptides obtained therefrom. These peptides correspond to amino acids 551-577, 594-603, 621-630, 657-679, and 719-758 of the LAV envelope glycoprotein. These peptides should provide suitable diagnostic reagents for the detection LAV-specific antibodies and for the generation of LAV-specific immunological reagents.

摘要翻译： 公开了一种新型人类逆转录病毒（称为淋巴结病相关病毒）或LAV的分子克隆和表征。 LAV最初与获得性免疫缺陷综合征（AIDS）的患者分离。 使用克隆的LAV互补DNA（cDNA）筛选由LAV感染的T淋巴细胞的基因组DNA构建的重组噬菌体文库。 通过M13霰弹枪克隆和双脱氧链终止测序方法确定从重组噬菌体克隆lambdJ19获得的插入物的核苷酸序列。 鉴定了env编码区，并由其获得各种亲水性肽。 这些肽对应于LAV包膜糖蛋白的氨基酸551-577,594-603,621-630,657-679和719-758。 这些肽应为检测LAV特异性抗体和产生LAV特异性免疫试剂提供合适的诊断试剂。