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公开(公告)号：US5866004A
公开(公告)日：1999-02-02
申请号：US524916
申请日：1995-09-08
申请人： Raymond K. Houck , Douglas J. Koebler , Glen P. Williams , Kenneth J. Kato , Robert D. Parks , Paul A. Bauer, Jr.
发明人： Raymond K. Houck , Douglas J. Koebler , Glen P. Williams , Kenneth J. Kato , Robert D. Parks , Paul A. Bauer, Jr.
CPC分类号： G01N1/34 , B01D11/0203 , G01N2001/4061 , G01N2030/062 , G01N33/24 , Y10T436/255
摘要： A supercritical fluid extraction (SFE) system. The SFE system includes a mechanism for providing supercritical fluid for extracting analyte from a sample. The SFE system also includes an extraction vessel and a mechanism for moving the extraction vessel into fluidic communication with the SFE system so that supercritical fluid can flow through the extraction vessel and extract analyte from the sample. Preferably, the SFE system is additionally comprised of a mechanism for holding a plurality of extraction vessels and an extraction chamber assembly. In this design, the moving mechanism selectively moves an extraction vessel from the holding mechanism into the extraction chamber in fluidic communication with the SFE system. Preferably, the SFE system further includes a computer for controlling the functions of the SFE system. The computer, for instance, could automatically and sequentially control the supercritical fluid extractions of the samples within the holding mechanism. The SFE system preferably includes a mechanism for collecting analyte from the fluid with analyte. The collecting mechanism is in fluid communication with extraction chamber assembly and preferably includes a restrictor for controlling the flow of fluid with analyte to the collecting mechanism. The restrictor converts the fluid with analyte from a first supercritical pressure to a second pressure which is less than the first supercritical pressure.
摘要翻译： 超临界流体萃取（SFE）系统。 SFE系统包括提供用于从样品中提取分析物的超临界流体的机制。 SFE系统还包括提取容器和用于将提取容器移动到与SFE系统流体连通的机构，使得超临界流体可以流过提取容器并从样品中提取分析物。 优选地，SFE系统还包括用于保持多个提取容器和提取室组件的机构。 在该设计中，移动机构选择性地将提取容器从保持机构移动到与SFE系统流体连通的提取室中。 优选地，SFE系统还包括用于控制SFE系统的功能的计算机。 例如，计算机可以自动和顺序地控制保持机构内的样品的超临界流体萃取。 SFE系统优选地包括用于从具有分析物的流体收集分析物的机构。 收集机构与提取室组件流体连通，并且优选地包括限制器，用于控制具有分析物的流体与收集机构的流动。 限流器将具有分析物的流体从第一超临界压力转换为小于第一超临界压力的第二压力。
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公开(公告)号：US5954954A
公开(公告)日：1999-09-21
申请号：US879336
申请日：1997-06-20
申请人： Raymond K. Houck , Douglas J. Koebler , Glen P. Williams , Joseph M. Levy , Victor G. Danielson
发明人： Raymond K. Houck , Douglas J. Koebler , Glen P. Williams , Joseph M. Levy , Victor G. Danielson
CPC分类号： G01N1/34 , B01D11/0203 , G01N2001/4061 , G01N2030/062 , G01N33/24
摘要： The present invention pertains to an apparatus for gathering analyte. The apparatus comprises a pumping mechanism for pumping solvent at supercritical pressure. The apparatus also comprises an extraction mechanism which receives a first sample and at least a second sample separate and distinct from the first sample and extract analyte from them essentially simultaneously. The extraction mechanism is connected to the pump mechanism to receive solvent at supercritical pressure for extracting the analyte from the samples. The apparatus additionally comprises a collection mechanism for collecting analyte from the solvent after the analyte has been extracted from the samples. The collection mechanism receives the supercritical solvent having the analyte extracted from the samples from the extraction mechanism. The present invention pertains to a method for extracting analyte. The method comprises the steps of pumping solvent at a supercritical pressure. Then there is the step of extracting analyte from N samples essentially simultaneously with the solvent at a supercritical pressure, where N is greater than or equal to two and is an integer. The N samples are separate and distinct from each other. Then there is the step of collecting the analyte of the N samples from the solvent.
摘要翻译： 本发明涉及用于收集分析物的装置。 该装置包括用于在超临界压力下泵送溶剂的泵送机构。 该装置还包括提取机构，其接收第一样品和至少第二样品，其与第一样品分离并且不同，并且基本上同时从其中提取分析物。 提取机构连接到泵机构以在超临界压力下接收溶剂以从样品中提取分析物。 该装置另外包括用于在从样品中提取分析物之后从溶剂收集分析物的收集机构。 收集机构接收来自提取机构的样品提取的分析物的超临界溶剂。 本发明涉及提取分析物的方法。 该方法包括以超临界压力泵送溶剂的步骤。 然后，在超临界压力下，基本上与溶剂同时从N个样品中提取分析物，其中N大于或等于2，并且为整数。 N个样本是分开的并且彼此不同。 然后是从溶剂中收集N个样品的分析物的步骤。
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公开(公告)号：US5750029A
公开(公告)日：1998-05-12
申请号：US388274
申请日：1995-02-13
申请人： Raymond K. Houck , Douglas J. Koebler , Glen P. Williams , Joseph M. Levy , Victor G. Danielson
发明人： Raymond K. Houck , Douglas J. Koebler , Glen P. Williams , Joseph M. Levy , Victor G. Danielson
CPC分类号： G01N1/34 , B01D11/0203 , G01N2001/4061 , G01N2030/062 , G01N33/24
摘要： The present invention pertains to an apparatus for gathering analyte. The apparatus comprises a pumping mechanism for pumping solvent at supercritical pressure. The apparatus also comprises an extraction mechanism which receives a first sample and at least a second sample separate and distinct from the first sample and extract analyte from them essentially simultaneously. The extraction mechanism is connected to the pump mechanism to receive solvent at supercritical pressure for extracting the analyte from the samples. The apparatus additionally comprises a collection mechanism for collecting analyte from the solvent after the analyte has been extracted from the samples. The collection mechanism receives the supercritical solvent having the analyte extracted from the samples from the extraction mechanism. The present invention pertains to a method for extracting analyte. The method comprises the steps of pumping solvent at a supercritical pressure. Then there is the step of extracting analyte from N samples essentially simultaneously with the solvent at a supercritical pressure, where N is greater than or equal to two and is an integer. The N samples are separate and distinct from each other. Then there is the step of collecting the analyte of the N samples from the solvent.
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公开(公告)号：US06830931B2
公开(公告)日：2004-12-14
申请号：US10032661
申请日：2001-10-26
申请人： Alfred Blalock Bahnson , Douglas J. Koebler , Charalambos N. Arthanassiou , Raymond K. Houck , Kris Sachsenmeier , Lei Qian
发明人： Alfred Blalock Bahnson , Douglas J. Koebler , Charalambos N. Arthanassiou , Raymond K. Houck , Kris Sachsenmeier , Lei Qian
IPC分类号： G01N3348
CPC分类号： C12Q1/24 , B01L7/02 , B01L9/00 , G01N15/1475 , G01N35/10 , G01N2015/1443 , G01N2015/1479 , G01N2015/1497 , G01N2035/00574 , Y10T436/101666
摘要： An apparatus for monitoring proteins and cells. The apparatus includes a plate having wells in which cells are disposed. The apparatus includes means for analyzing the effect or proteins and other biological and chemical moieties on the cells. A method for monitoring of proteins and cells. A method for analyzing a cells. An apparatus for aligning light in a well of a plate for holding cells. A method for lighting a well. A method for determining a condition of a cell. An apparatus for indicating a condition of a cell. A method for establishing a focus profile of a plate having wells for holding cells. A method for manipulating cells. An apparatus for manipulating cells.
摘要翻译： 用于监测蛋白质和细胞的装置。 该装置包括具有其中设置有电池的孔的板。 该装置包括用于分析细胞上的效应或蛋白质和其他生物和化学部分的装置。 一种监测蛋白质和细胞的方法。 分析细胞的方法。 一种用于对准用于保持电池的板的孔中的光的装置。 一种照明井的方法。 一种确定细胞条件的方法。 一种用于指示单元的状态的装置。 一种用于建立具有用于保持电池的孔的板的焦点分布的方法。 一种操纵细胞的方法。 一种用于操纵电池的装置。
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公开(公告)号：US08859263B2
公开(公告)日：2014-10-14
申请号：US13568900
申请日：2012-08-07
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/16 , G02B21/34 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且在环境被动态控制并保持在所需条件下可以检测细胞。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的环境中孵育细胞，该环境被保持在期望的状态，并且其中可以在环境被动态地控制并保持在所需条件下细胞被检查。 此外，存在确定细胞状态的步骤。
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公开(公告)号：US20110195055A1
公开(公告)日：2011-08-11
申请号：US12930471
申请日：2011-01-07
IPC分类号： A61K35/12 , C12M1/34 , C12M1/36 , C12N5/071 , C12Q1/02 , C12N5/09 , C12N5/0789 , A61P37/00 , A61P7/00
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/16 , G02B21/34 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且其中细胞可以在环境被动态控制并保持在所需条件下被检查。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的环境中孵育细胞，该环境被保持在期望的状态，并且其中可以在环境被动态地控制并保持在所需条件下细胞被检查。 此外，存在确定细胞状态的步骤。
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7.发明授权Method for collecting analyte and an orifice for reducing pressure of a fluid 失效用于收集分析物和用于减少流体压力的孔口的方法
公开(公告)号：US5453198A
公开(公告)日：1995-09-26
申请号：US993295
申请日：1992-12-21
CPC分类号： G01N1/34 , B01D11/0203 , G01N7/04 , G01N2001/4061 , G01N2030/025 , G01N2030/062 , G01N30/02 , Y10T436/25875
摘要： An orifice which includes a housing having a low pressure side with a first bore hole and a second bore hole. The low pressure side has a first channel and a second channel in fluidic communication with the first bore hole and the second bore hole, respectively. The housing also has a high pressure side with a third bore hole. The orifice also includes an adapter which has a tip, a fourth bore hole and a third channel in fluidic communication therewith. The third channel has a constant diameter for maintaining an essentially constant pressure thereacross when pressure is applied thereto such that fluid flowing through the third channel does not solidify. The adapter is sealingly received by the third bore hole such that the third channel and the first channel align. Additionally, the housing includes an interface plate disposed in the housing between the third channel and the first channel. The plate sealingly receives the tip of the adapter and allows for flow to exist between the third channel and the first channel through a plate hole. A method for collecting analyte. The method includes the steps of providing analyte obtained from supercritical fluid extraction to a collection trap through a high pressure side of an orifice.
摘要翻译： 一种孔口，包括具有低压侧的壳体，其具有第一钻孔和第二钻孔。 低压侧分别具有与第一钻孔和第二钻孔流体连通的第一通道和第二通道。 壳体还具有具有第三钻孔的高压侧。 孔口还包括具有尖端，第四钻孔和与其流体连通的第三通道的适配器。 第三通道具有恒定的直径，用于在施加压力时保持基本恒定的压力，使得流过第三通道的流体不固化。 适配器被第三钻孔密封地接收，使得第三通道和第一通道对准。 此外，壳体包括设置在第三通道和第一通道之间的壳体中的接口板。 板密封地接收适配器的尖端，并允许流动通过板孔存在于第三通道和第一通道之间。 收集分析物的方法。 该方法包括以下步骤：通过孔的高压侧将从超临界流体萃取获得的分析物提供到收集阱。
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公开(公告)号：US5205987A
公开(公告)日：1993-04-27
申请号：US662255
申请日：1991-02-28
CPC分类号： G01N1/34 , B01D11/0203 , G01N7/04 , G01N2001/4061 , G01N2030/025 , G01N2030/062 , G01N30/02 , Y10T436/25875
摘要： The present invention pertains to an off-line collection system for use with supercritical fluid extraction. The system is comprised of mechanism for collecting analyte associated with the supercritical fluid extraction. The system is additionally comprised of an orifice for controlling flow of analyte to the collecting mechanism. The orifice converts analyte at a high pressure to a lower pressure. The orifice has a high pressure side which receives said high pressure analyte, and a lower pressure side in fluidic communication with the high pressure side which receives said high pressure analyte after it has been converted to a lower pressure. The high pressure side is capable of transporting analyte thereacross without essentially any loss thereof to the low pressure side which is in fluidic communication with the collecting mechanism. The analyte experiences essentially constant pressure as it is transported across the high pressure side. The system is also comprised of mechanism for desorbing the analyte from the collection mechanism. The desorbing mechanism is in fluidic communication with the collecting mechanism. In a preferred embodiment, the system includes mechanism for purging the analyte. The purging mechanism is in fluidic communication with the collecting mechanism. Additionally, the collecting mechanism preferably includes a collection trap in which analyte is gathered, and mechanism for controlling the flow of analyte into the collection trap. The flow controlling mechanism is in fluidic communication with the collection trap and the purging mechanism. The flow control mechanism preferably includes an orifice.
摘要翻译： 本发明涉及一种用于超临界流体萃取的离线收集系统。 该系统由用于收集与超临界流体萃取物相关的分析物的机构组成。 该系统还包括用于控制分析物流到收集机构的孔口。 孔口将分析物在高压下转换成较低的压力。 孔口具有高压侧，其接收所述高压分析物，以及与高压侧流体连通的低压侧，其在将其转换成较低压力之后接收所述高压分析物。 高压侧能够将分析物在其上传输而基本上不会损失到与收集机构流体连通的低压侧。 分析物在高压侧运输时，基本上保持恒定的压力。 该系统还包括从收集机构解吸分析物的机理。 解吸机构与收集机构流体连通。 在优选实施例中，该系统包括用于清除分析物的机构。 清洗机构与收集机构流体连通。 此外，收集机构优选地包括其中收集分析物的收集阱，以及用于控制分析物流入收集阱的机构。 流量控制机构与收集阱和清洗机构流体连通。 流量控制机构优选地包括孔口。
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公开(公告)号：US20140371112A1
公开(公告)日：2014-12-18
申请号：US14476272
申请日：2014-09-03
IPC分类号： G01N33/50
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/16 , G02B21/34 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且在环境被动态控制并保持在所需条件下可以检测细胞。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的环境中孵育细胞，该环境被保持在期望的状态，并且其中可以在环境被动态地控制并保持在所需条件下细胞被检查。 此外，存在确定细胞状态的步骤。
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公开(公告)号：US6008010A
公开(公告)日：1999-12-28
申请号：US741628
申请日：1996-11-01
IPC分类号： G01N33/48 , C12M1/00 , C12M1/34 , C12M1/36 , C12M3/00 , C12N1/04 , C12N5/06 , C12Q1/02 , C12P1/00 , A01N1/02 , C12N1/00
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/34 , G02B21/16 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled closed environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled closed environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的闭合环境的细胞的机制，其中细胞生长，其被保持在期望的条件，并且其中细胞可以在环境被动态控制并保持在所需条件下被检查。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的闭合环境中孵育细胞，该环境保持在期望的状态，并且其中可以在环境被动态控制并保持在所需条件下细胞进行检查。 此外，存在确定细胞状态的步骤。
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