公开(公告)号：US5427663A

公开(公告)日：1995-06-27

申请号：US74432

申请日：1993-06-08

申请人： Robert H. Austin ,  Wayne D. Volkmuth ,  Lynn C. Rathburn

发明人： Robert H. Austin ,  Wayne D. Volkmuth ,  Lynn C. Rathburn

IPC分类号： B01J19/00 ,  B01L3/00 ,  C12M1/42 ,  C12M3/08 ,  G01N15/02 ,  G01N15/14 ,  G01N27/447 ,  G01N30/60 ,  G01N30/92 ,  G01N27/26 ,  B03B5/00

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01L3/502753 ,  B01L3/502761 ,  C12M47/04 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0421 ,  B01L2400/086 ,  G01N15/0255 ,  G01N2015/149 ,  G01N30/6095 ,  G01N30/92

摘要： A sorting apparatus and method for fractionating and simultaneously viewing individual microstructures, such as free cells, viruses, macromolecules, or minute particles in a fluid medium. The sorting apparatus is composed of a substrate having a receptacle located therein, the receptacle having sidewalls and a floor. An array of obstacles is positioned within the receptacle with the obstacles upstanding from the floor of the receptacle. A transparent cover overlies the array of obstacles to cover the receptacle and afford visual observation of migration of the microstructures exclusively through the array of obstacles. Electrodes may be positioned within the receptacle to generate an electric field in the fluid medium in the receptacle in order to induce the migration of the microstructures. Migration of the microstructures may also occur, for example, by a hydrodynamic field, an optical field, a magnetic field, or a gravity field applied to the receptacle. The obstacles of the array of obstacles may be of various shapes such as round posts, rectangular bunkers, or v-shaped or cup-shaped structures. The arrays of obstacles are formed of a predetermined and reproducible pattern, and can be reused. Methods for manufacturing and using the apparatus are also claimed.

摘要翻译： 一种用于分级并同时观察流体介质中的各种微结构（例如游离细胞，病毒，大分子或微小颗粒）的分选装置和方法。 分拣装置由具有位于其中的插座的基板构成，该插座具有侧壁和底板。 障碍物阵列位于容器内，其中障碍物从容器的地板竖立起来。 透明盖子覆盖障碍物阵列以覆盖容器，并提供目视观察微观结构仅通过障碍物阵列的迁移。 电极可以位于容器内以在容器中的流体介质中产生电场，以引起微结构的迁移。 微结构的移动也可以例如通过施加到容器的流体动力场，光场，磁场或重力场来发生。 障碍物阵列的障碍物可以是各种形状，例如圆柱，矩形掩体或v形或杯形结构。 障碍物阵列由预定和可再现的图案形成，并且可以重复使用。 还要求保护制造和使用该装置的方法。