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公开(公告)号：US20130071879A1
公开(公告)日：2013-03-21
申请号：US13580336
申请日：2011-02-23
CPC分类号： C12P19/34 , C09K3/00 , C12Q1/686 , C12Q2565/518 , C12Q2527/137
摘要： Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimising GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilising flow cells.
摘要翻译： 提供了扩增核酸的方法。 这些方法可以用于最小化由某些核酸序列的优先扩增引起的序列特异性偏差。 描述了可以降低AT富含模板相对于GC富集模板的效率的方法，由此使具有不同序列的多个模板的扩增反应期间的GC偏差最小化。 该方法适用于固相扩增，例如利用流动池。
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公开(公告)号：US08759037B2
公开(公告)日：2014-06-24
申请号：US13580336
申请日：2011-02-23
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C09K3/00 , C12Q1/686 , C12Q2565/518 , C12Q2527/137
摘要： Methods for amplifying nucleic acids are provided. The methods can be used to minimize sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimizing GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilizing flow cells.
摘要翻译： 提供了扩增核酸的方法。 这些方法可以用于最小化由某些核酸序列的优先扩增引起的序列特异性偏差。 描述了可以降低AT富含模板相对于GC富集模板的效率的方法，由此使具有不同序列的多个模板的扩增反应期间的GC偏差最小化。 该方法适用于固相扩增，例如利用流动池。
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公开(公告)号：US08999642B2
公开(公告)日：2015-04-07
申请号：US13387078
申请日：2009-08-25
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译： 本发明提供了控制固体支持物表面上不同分子种类的密度的方法。 将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。 衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。 一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。 在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。
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公开(公告)号：US20120122737A1
公开(公告)日：2012-05-17
申请号：US13387078
申请日：2009-08-25
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译： 本发明提供了控制固体支持物表面上不同分子种类的密度的方法。 将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。 衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。 一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。 在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。
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公开(公告)号：US20090226975A1
公开(公告)日：2009-09-10
申请号：US12395299
申请日：2009-02-27
IPC分类号： C12P19/34
CPC分类号： C12Q1/6837 , C12Q2565/543
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译： 本发明提供了控制固体支持物表面上不同分子种类的密度的方法。 将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。 衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。 一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。 在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。
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公开(公告)号：US08945835B2
公开(公告)日：2015-02-03
申请号：US13444654
申请日：2012-04-11
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译： 本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。
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公开(公告)号：US08741571B2
公开(公告)日：2014-06-03
申请号：US13801144
申请日：2013-03-13
CPC分类号： C12N15/1068 , B01J19/0046 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/537 , C12Q2563/149 , C12Q2563/161
摘要： The present invention provides a method of creating an array of features. The method can include steps of (a) providing a plurality of beads, wherein each bead in the plurality of beads includes probe content; (b) contacting the plurality of beads with a surface to produce a layer of beads on the surface; and (c) transferring the probe content from the beads to the surface to create an array of spatially discrete features on the surface, wherein each spatially discrete feature includes probe content from a bead in the plurality of beads.
摘要翻译： 本发明提供了一种创建特征阵列的方法。 该方法可以包括以下步骤：（a）提供多个珠，其中多个珠中的每个珠包括探针含量; （b）使多个珠粒与表面接触以在表面上产生一层珠粒; 和（c）将探针含量从珠粒转移到表面以在表面上产生空间上离散的特征的阵列，其中每个空间上离散的特征包括来自多个珠粒中的珠粒的探针含量。
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公开(公告)号：US20130281306A1
公开(公告)日：2013-10-24
申请号：US13881320
申请日：2011-10-26
申请人： Roberto Rigatti , Jonathan Mark Boutell , Jason Richard Betley , Niall Anthony Gormley , Mostafa Ronaghi , Dirk Evers
发明人： Roberto Rigatti , Jonathan Mark Boutell , Jason Richard Betley , Niall Anthony Gormley , Mostafa Ronaghi , Dirk Evers
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2565/514 , C12Q2537/155 , C12Q2525/186 , C12Q2525/185 , C12Q2537/113 , C12Q2525/204
摘要： The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to nucleic acid sequencing.
摘要翻译： 本技术涉及分子科学，如基因组学。 更具体地，本技术涉及核酸测序。
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公开(公告)号：US20120053063A1
公开(公告)日：2012-03-01
申请号：US13219422
申请日：2011-08-26
IPC分类号： C40B20/00
CPC分类号： C12Q1/6874 , C12Q2535/119 , C12Q2535/122 , C12Q2565/513 , C12Q2565/543
摘要： Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.
摘要翻译： 本文提供了用于测序多核苷酸分子的方法。 该方法包括提供连接到表面的多个多核苷酸分子的步骤，其中每个多核苷酸分子的第一部分连接到该表面的第一位置，并且每个多核苷酸分子的第二部分连接到该多核苷酸分子的第二位置 表面，第一和第二位置的相对接近度与第一和第二部分配对的概率相关，分离表面上的多核苷酸分子的第一和第二部分，确定第一和第二部分的序列 多核苷酸分子，并比较相对接近度和序列以确定哪个第一和第二部分配对并确定靶多核苷酸分子的序列。
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公开(公告)号：US20100022412A1
公开(公告)日：2010-01-28
申请号：US12497397
申请日：2009-07-02
CPC分类号： C12N15/1068 , B01J19/0046 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/537 , C12Q2563/149 , C12Q2563/161
摘要： The present invention provides methods for creating an array of features on a surface based on content transferred from a plurality of beads to the surface. Nucleic acid content can be transferred using a method including the steps of (a) providing a surface having one or more primer oligonucleotides attached to the surface; (b) providing a pool of beads, wherein beads in the pool have a plurality of templates attached thereto, the plurality comprising multiple copies of a single nucleic acid template sequence; (c) arraying the beads onto the surface by hybridizing the templates to the primer oligonucleotides; and (d) extending the primers to produce copies of the templates attached to the surface.
摘要翻译： 本发明提供了基于从多个珠粒转移到表面的内容来在表面上创建特征阵列的方法。 可以使用包括以下步骤的方法转移核酸含量：（a）提供具有附着于所述表面的一种或多种引物寡核苷酸的表面; （b）提供珠池，其中所述池中的珠子具有连接到其上的多个模板，所述多个模板包含单个核酸模板序列的多个拷贝; （c）通过将模板与引物寡核苷酸杂交将珠子排列在表面上; 和（d）扩展引物以产生附着在表面上的模板的拷贝。
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