公开(公告)号：US20050157386A1

公开(公告)日：2005-07-21

申请号：US11020387

申请日：2004-12-22

申请人： Roger Greenwald ,  James Zavislan

发明人： Roger Greenwald ,  James Zavislan

IPC分类号： B01L3/00 ,  G01N1/36 ,  G01N21/03 ,  G02B21/00 ,  G02B21/34 ,  A61D1/02 ,  A61B17/22

CPC分类号： G01N21/0303 ,  B01L3/508 ,  B01L2300/043 ,  B01L2300/0609 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0822 ,  B01L2400/0683 ,  G01N1/36 ,  G01N2021/0339 ,  G01N2021/0342 ,  G01N2021/0364 ,  G02B21/0028 ,  G02B21/34

摘要： A tray or holder for tissue specimens, especially of excised tissue, such as biopsied specimens, is used with a confocal imaging system, especially a laser scanning confocal microscope system. The tray may be disposable after imaging of the specimen carried therein or may archive the specimen. A window supports the specimen. Clamps mounted inside the tray restrain the tissue. A compliant bag is mounted outside the tray on one side of a window of the tray on which the specimen is disposed. During imaging the specimen is immersed in a liquid contained in the tray having an index of refraction which closely matches the index of refraction of the tissue. The bag also contains an index matching liquid preferably having the same index as the liquid in the tray. A stabilizing plate is attached to a surface of the bag which faces the window. An objective lens of the confocal imaging system receives and supports the plate, preferably by a magnetic coupling ring around the plate which is magnetically attached to the barrel of the objective lens. The lens may be fixed and a mechanism for positioning the tray with respect to the lens in a direction along the optical axis of the lens and in orthogonal directions perpendicular to that axis shares a common support with the lens. Wavefront distortion which may result from an effectively corrugated surface of the specimen is minimized by selecting an immersion liquid which equals the refractive index of the near surface tissues. The tissue determines the index of the immersion fluid. The thicknesses and refractive indices of the window and plate are then determined to correct the residual aberration of the optical system. Different trays having different window and plate thicknesses and indices may be provided for different specimen species such as kidney, liver, cervix, et cetera. Thus, a generic confocal imaging system with a generic objective lens is corrected for image aberration and wavefront distortion due to the surface corrugations of the tissue. The correction is simplified since the bag is compliant and compresses so that the optical thickness along the axis between the objective lens and the focal plane in or at the specimen is substantially invariant whether the beam is focused at the interface between the specimen and the window or within the specimen over a focusing range which can extend over several millimeters.

公开(公告)号：US20070195308A1

公开(公告)日：2007-08-23

申请号：US11788920

申请日：2007-04-23

申请人： James Zavislan ,  Roger Greenwald

发明人： James Zavislan ,  Roger Greenwald

IPC分类号： G01N1/00 ,  G01J1/42

CPC分类号： G02B21/0024 ,  A61B10/0096 ,  G01N1/36 ,  G02B21/34

摘要： In order to determine, rapidly and without the delay required by conventional tissue preparation techniques for pathological examination (freezing, sectioning, staining, etc.), whether, an excision, which may be a biopsy sample, is representative of the morphology of interest of whether an excisional biopsy in which the tissue taken completely removes the abnormality is, in either case, the tissue which is desired to be excisioned, the tissue specimen (18) in encapsulated, preferably as part of the biopsy procedure. The encapsulated tissue is contained in an optically transparent cassette (34). The cassette (34) or an endcap (38) enclosing the cassette is marked with a fiducial (40) indicating and corresponding to the location of the excision on the patient's body. An image, which is preferably a representation of a surface of the tissue specimen and the vertical section(s) area of the tissue internal of the specimen and adjacent to a surface thereof, is obtained by means of an electro-optical imaging system (10), preferably a confocal laser scanning microscope. The cassette is moved, preferably in a stage (22) which rotates the cassette while translating it, so that the head (12) of the confocal microscope (its objective lens) provides a linear scan in a direction perpendicular to the wall of the cassette (also perpendicular to the surface of the tissue encapsulated in the cassette). The display (28) from the microscope indicates the morphology at and in proximity to the surface of the specimen as well as the location thereof. The pathologist and the surgeon is thereby provided with information concerning the specimen and whether the entire abnormality desired to be removed has indeed been removed and/or whether the biopsy is representative of the body region of interest in the case of a biopsy sample.

公开(公告)号：US20070206275A1

公开(公告)日：2007-09-06

申请号：US11357234

申请日：2006-02-17

申请人： Paul Hemmer ,  Zachary Eastman ,  Jay Eastman ,  William Fox ,  James Zavislan

发明人： Paul Hemmer ,  Zachary Eastman ,  Jay Eastman ,  William Fox ,  James Zavislan

IPC分类号： G02B21/06

CPC分类号： G02B21/0012 ,  G02B21/0024 ,  G02B21/365

摘要： A system for macroscopic and confocal imaging of tissue having a macroscopic imager for capturing a macroscopic image of the tissue's surface, a confocal imager for capturing one or more optically formed sectional microscopic images on or within tissue, a computer for receiving images from such imagers, and a tissue attachment device in which the macroscopic imager and confocal imager are each individually presented to the tissue utilizing the tissue attachment device in a predefined alignment, such that imaging locations of the confocal imager with respect to the tissue surface spatially correlate with macroscopic image. A user interface is operable on the computer to enable display of the macroscopic image on a display coupled to the computer, and to indicate a region within the macroscopic image associated with the field of view of the tissue imagable by the confocal imager. The user interface enables graphical tracking and targeting of imaging locations of the confocal imager in macroscopic image, and marking on the macroscopic image of the locations of confocal images with respect to the tissue surface.

摘要翻译： 一种具有用于捕获组织表面的宏观图像的宏观成像器的组织的宏观和共焦成像系统，用于捕获组织上或组织内的一个或多个光学形成的截面微观图像的共焦成像器，用于从这样的成像器接收图像的计算机， 以及组织附着装置，其中宏观成像器和共聚焦成像器各自以预定对准单独地呈现给组织，使得共焦成像器相对于组织表面的成像位置在空间上与宏观图像相关。 用户界面在计算机上可操作以使得能够在耦合到计算机的显示器上显示宏观图像，并且指示与由共焦成像器可成像的组织的视场相关联的宏观图像内的区域。 用户界面能够在宏观图像中进行图像跟踪和共焦成像器的成像位置的瞄准，并且在相对于组织表面的共聚焦图像的位置的宏观图像上进行标记。

公开(公告)号：US09072430B2

公开(公告)日：2015-07-07

申请号：US13235848

申请日：2011-09-19

申请人： Andrew Berger ,  Thomas Foster ,  Rebecca Wilson ,  James Zavislan

发明人： Andrew Berger ,  Thomas Foster ,  Rebecca Wilson ,  James Zavislan

IPC分类号： A61B5/00

CPC分类号： A61B5/0095

摘要： An apparatus for determining the location of a radio-opaque marker embedded in an excised specimen includes a radio-opaque marker including an energy beacon that can be embedded in the specimen, an energy beacon controller, at least three detectors, a signal processing component, and a display device. An associated method is disclosed. A method for evaluating a radiographically labeled excised specimen involves determining the location of a radio-opaque marker embedded in the excised specimen, anatomically sectioning the excised specimen, macro-optically segmenting the sectioned specimen into an adipose tissue component, a fibrous tissue component, and an epithelial tissue component, and microscopically examining at least one of the tissue components. An associated apparatus is disclosed.

摘要翻译： 一种用于确定嵌入在切除的标本中的不透射线标记的位置的装置包括：不透射线标记，其包括可嵌入样本中的能量信标，能量信标控制器，至少三个检测器，信号处理部件， 和显示装置。 公开了一种相关联的方法。 用于评估放射学标记的切除标本的方法包括确定嵌入切除的样本中的不透射线标记的位置，解剖切片切除的样本，将分割的样本宏观分割成脂肪组织成分，纤维组织成分和 上皮组织成分，并显微检查组织成分中的至少一个。 公开了一种相关装置。

公开(公告)号：US20070070497A1

公开(公告)日：2007-03-29

申请号：US11601203

申请日：2006-11-17

申请人： Jay Eastman ,  Milind Rajadhyaksha ,  James Zavislan

发明人： Jay Eastman ,  Milind Rajadhyaksha ,  James Zavislan

IPC分类号： G02B21/06

CPC分类号： G02B21/0068 ,  A61K49/001 ,  G01N33/5743

摘要： A system using cross polarization effects and an enhancement agent having citric or other similar alpha hydroxy acid to enhance confocal microscope reflectance images and particularly images of the nuclei of BCCs (basal cell carcinomas) and SCCs (squamous cell carcinomas) in the confocal reflectance images of excised tumor slices obtained during Mohs surgery by illuminating the tissue being imaged (a tumor slice) using polarized light. The reflected illumination is passed to a polarization analyzer, which passes the polarization component which is crossed with respect to the polarization of the illuminating light. The light from the analyzer is passed through the confocal aperture and detected. The section of the tissue either at the surface or within the tissue is scanned and the reflectance image is produced with enhanced visualization of the cellular or nuclear structure thereof thereby enabling determination of the extent of the tumor (cancerous cells) in the section. A method is also provided using the system for diagnosing cancerous cells in skin tissue.

摘要翻译： 使用交叉极化效应的系统和具有柠檬酸或其它类似α羟基酸的增强剂来增强共聚焦显微镜反射图像，特别是BCC（基底细胞癌）和SCC（鳞状细胞癌）的核的图像在共聚焦反射图像中 通过使用偏振光照射被成像的组织（肿瘤切片）在Mohs手术期间获得的切除的肿瘤切片。 反射的照明被传递到偏振分析器，偏振分析器使相对于照明光的偏振交叉的偏振分量通过。 来自分析仪的光通过共焦孔并检测。 扫描在表面或组织内的组织的部分，并且通过细胞或核结构的增强的可视化产生反射图像，从而能够确定该部分中肿瘤（癌细胞）的程度。 还提供了一种用于诊断皮肤组织中的癌细胞的系统的方法。

公开(公告)号：US20060268274A1

公开(公告)日：2006-11-30

申请号：US11499582

申请日：2006-08-04

申请人： Milind Rajadhyaksha ,  James Zavislan

发明人： Milind Rajadhyaksha ,  James Zavislan

IPC分类号： G01J4/00

CPC分类号： G01N21/21 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4735 ,  G01N2021/4792 ,  G02B21/0032 ,  G02B21/0048 ,  G02B21/0068 ,  G02B21/14 ,  G02B21/33 ,  G02B21/36

摘要： A confocal scanning microscope system (10) using cross polarization effects and an enhancement agent (acetic acid) to enhance confocal microscope reflectance images of the nuclei of BCCs (basal cell carcinomas) and SCCs (squamous cell carcinomas) in the confocal reflectance images of excised tumor slices. The confocal scanning microscope system having a laser (11) for generating an illumination beam (12), a polygon mirror (18) for scanning the beam to a tissue sample (22) and for receiving a returned beam from the tissue sample and detector (28) for detecting the returned beam to form an image. The system further includes a half-waveplate (13) having a rotatable stage (14) and a quarter-wave plate (21) having a rotatable stage (20) disposed in the optical path of the illumination beam and at least a linear polarizer (24) having a rotatable stage (25) disposed in the optical path of the returned beam from the tissue sample.

摘要翻译： 使用交叉极化效应的共聚焦扫描显微镜系统（10）和增强剂（乙酸）以增强切除的共聚焦反射图像中BCC（基底细胞癌）和SCC（鳞状细胞癌）细胞核的共聚焦显微镜反射图像 肿瘤切片。 具有用于产生照明光束（12）的激光（11）的共焦扫描显微镜系统，用于将光束扫描到组织样本（22）并用于从组织样本和检测器（22）接收返回光束的多面镜（18） 28），用于检测返回的光束以形成图像。 该系统还包括具有可旋转台（14）和四分之一波片（21）的半波片（13），其具有设置在照明光束的光路中的可旋转台（20）和至少一个线性偏振器 24）具有设置在来自组织样本的返回光束的光路中的可旋转台（25）。

公开(公告)号：US20050101871A1

公开(公告)日：2005-05-12

申请号：US11013099

申请日：2004-12-15

申请人： James Zavislan ,  Jay Eastman

发明人： James Zavislan ,  Jay Eastman

IPC分类号： A61B5/00 ,  A61B5/103 ,  A61B6/00

CPC分类号： A61B5/444 ,  A61B5/0059 ,  A61B5/0064 ,  A61B5/0068 ,  A61B2562/146

摘要： A handheld confocal imaging system for in vivo observation of dermal and subdermal tissue allows diagnosis of conditions substantially beneath the surface of the skin. A confocal head has optics which scan the tissue so as to provide images of vertical sections of the tissue. Both two and three dimensional imaging may be provided for diagnosis and location of basal cell carcinomas and melanomas, and so as to enable visualization of tumor borders prior to excision.

摘要翻译： 用于体内观察皮肤和皮下组织的手持共聚焦成像系统允许诊断基本上在皮肤表面下方的状况。 共焦头具有扫描组织以便提供组织的垂直部分的图像的光学元件。 可以提供二维和三维成像用于诊断和定位基底细胞癌和黑素瘤，并且可以在切除前使肿瘤边界可视化。

公开(公告)号：US20070287921A1

公开(公告)日：2007-12-13

申请号：US11784128

申请日：2007-04-05

申请人： James Zavislan

发明人： James Zavislan

IPC分类号： A61B6/00

CPC分类号： A61B5/6842 ,  A61B5/0068 ,  A61B5/061 ,  A61B5/441 ,  A61B5/444 ,  A61B5/445 ,  A61B5/6834 ,  A61B5/6835 ,  A61B5/6843 ,  A61B5/704

摘要： An improved system for confocal imaging within dermal tissue of a patient is provided which minimizes instability in confocal images by reducing the relative motion of the tissue with respect to the confocal imaging optics of the system. The system includes a mechanism for maintaining an area of skin tissue under stress by application of force at the edges of the area, and an imaging head coupled to this mechanism for imaging the stressed skin. The mechanism includes a mechanical structure, such as a platen, brace, or attachment, which both supports the imaging head of the system and applies stress to a limited surface area of the tissue to minimize skin motion during confocal imaging.

摘要翻译： 提供了一种用于患者真皮组织内的共焦成像的改进系统，其通过减少组织相对于系统的共聚焦成像光学器件的相对运动而使共焦图像中的不稳定性最小化。 该系统包括用于通过在该区域的边缘处施加力来保持应力下的皮肤组织的面积的机构，以及耦合到该机构的用于对应力皮肤成像的成像头。 该机构包括机械结构，例如压板，支架或附件，其均支撑系统的成像头部，并将应力施加到组织的有限表面区域，以使共聚焦成像期间的皮肤运动最小化。

公开(公告)号：US20120325003A1

公开(公告)日：2012-12-27

申请号：US13235848

申请日：2011-09-19

申请人： Andrew Berger ,  Thomas Foster ,  Rebecca Wilson ,  James Zavislan

发明人： Andrew Berger ,  Thomas Foster ,  Rebecca Wilson ,  James Zavislan

IPC分类号： G01N21/84 ,  G01N29/04

CPC分类号： A61B5/0095

摘要： An apparatus for determining the location of a radio-opaque marker embedded in an excised specimen includes a radio-opaque marker including an energy beacon that can be embedded in the specimen, an energy beacon controller, at least three detectors, a signal processing component, and a display device. An associated method is disclosed. A method for evaluating a radiographically labeled excised specimen involves determining the location of a radio-opaque marker embedded in the excised specimen, anatomically sectioning the excised specimen, macro-optically segmenting the sectioned specimen into an adipose tissue component, a fibrous tissue component, and an epithelial tissue component, and microscopically examining at least one of the tissue components. An associated apparatus is disclosed.

摘要翻译： 一种用于确定嵌入在切除的标本中的不透射线标记的位置的装置包括：不透射线标记，其包括可嵌入样本中的能量信标，能量信标控制器，至少三个检测器，信号处理部件， 和显示装置。 公开了一种相关联的方法。 用于评估放射学标记的切除标本的方法包括确定嵌入切除的样本中的不透射线标记的位置，解剖切片切除的样本，将分割的样本宏观分割成脂肪组织成分，纤维组织成分和 上皮组织成分，并显微检查组织成分中的至少一个。 公开了一种相关装置。

公开(公告)号：US20050171441A1

公开(公告)日：2005-08-04

申请号：US11081181

申请日：2005-03-16

申请人： James Zavislan

发明人： James Zavislan

IPC分类号： A61B5/103 ,  A61B6/00

CPC分类号： A61B5/6842 ,  A61B5/0068 ,  A61B5/061 ,  A61B5/441 ,  A61B5/444 ,  A61B5/445 ,  A61B5/6834 ,  A61B5/6835 ,  A61B5/6843 ,  A61B5/704

摘要： An improved system for confocal imaging within dermal tissue of a patient is provided which minimizes instability in confocal images by reducing the relative motion of the tissue with respect to the confocal imaging optics of the system. The system includes a mechanism for maintaining an area of skin tissue under stress by application of force at the edges of the area, and an imaging head coupled to this mechanism for imaging the stressed skin. The mechanism includes a mechanical structure, such as a platen, brace, or attachment, which both supports the imaging head of the system and applies stress to a limited surface area of the tissue to minimize skin motion during confocal imaging.