公开(公告)号：US20060289786A1

公开(公告)日：2006-12-28

申请号：US11416886

申请日：2006-05-02

申请人： Roger Taylor ,  Taylor Reid ,  Howard Choi

发明人： Roger Taylor ,  Taylor Reid ,  Howard Choi

IPC分类号： G01N21/64 ,  G01J3/30

CPC分类号： G01J3/10 ,  G01J3/4406 ,  G01J2001/4242 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486

摘要： A system and method for a pulsed light source used in detecting fluorescence from a plurality of samples of biological material discretely, continuously or intermittently during thermal cycling of DNA to accomplish a polymerase chain reaction (PCR). An apparatus for sampling at least one sample of a biological material comprises a light source that emits a pulsed excitation light that interacts with the sample and a detector sensitive to fluorescence emitted from the sample. A method of sampling at least one sample to detect fluorescence comprises generating a pulsed excitation light with a pulsed light source; directing the pulsed excitation light into the sample; illuminating a sample with the pulsed excitation light to generate an emission light; and detecting the optical characteristics of the emission light.

摘要翻译： 脉冲光源的系统和方法，用于在DNA的热循环过程中离散地，连续地或间断地检测生物材料的多个样品的荧光以完成聚合酶链反应（PCR）。 用于对生物材料的至少一个样品进行采样的装置包括发射与样品相互作用的脉冲激发光的光源和对从样品发出的荧光敏感的检测器。 对至少一个样品进行采样以检测荧光的方法包括用脉冲光源产生脉冲激发光; 将脉冲激发光引导到样品中; 用脉冲激发光照射样品以产生发射光; 并检测发射光的光学特性。

公开(公告)号：US07459302B2

公开(公告)日：2008-12-02

申请号：US10261751

申请日：2002-10-01

申请人： Taylor Reid ,  Roger Taylor ,  Larry Brown

发明人： Taylor Reid ,  Roger Taylor ,  Larry Brown

IPC分类号： C12M1/38

CPC分类号： B01L7/52 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/1805 ,  Y10S435/809

摘要： The invention relates to an apparatus and methods for preventing condensation on the interior surfaces of sample tubes which are being exposed to temperature cycles, such as during a PCR amplification reaction. In particular, the invention relates to apparatus comprising a sample block comprising a plurality of sample wells for receiving sample tubes and heating elements disposed in the sample wells for heating at least a portion of the sides of sample tubes (e.g., at least the portion which forms the head space after a tube is filled with a PCR reaction mixture). In a preferred aspect, the sample block is part of a thermocycling device for performing PCR and the side-wall heater is used to enhance uniformity and speed of amplification reactions. For example, by decreasing or eliminating condensation, signal strength jumps in a real-time PCR assay can be minimized as can reaction non-homogeneity.

摘要翻译： 本发明涉及一种用于防止在诸如PCR扩增反应期间暴露于温度循环的样品管的内表面上的冷凝的装置和方法。 特别地，本发明涉及包括样品块的装置，其包括多个用于接收样品管的样品孔和设置在样品阱中的加热元件，用于加热样品管的至少一部分侧面（例如，至少部分 在管填充PCR反应混合物后形成顶部空间）。 在优选的方面，样品块是用于进行PCR的热循环装置的一部分，并且侧壁加热器用于增强扩增反应的均匀性和速度。 例如，通过减少或消除冷凝，实时PCR测定中的信号强度跳跃可以如反应非均匀性那样最小化。

公开(公告)号：US20070194247A1

公开(公告)日：2007-08-23

申请号：US11510520

申请日：2006-08-25

申请人： Taylor Reid ,  Roger Taylor

发明人： Taylor Reid ,  Roger Taylor

IPC分类号： G01J1/58 ,  G01T1/10 ,  H01L31/14 ,  H01J40/14 ,  F21V9/16

CPC分类号： G01N21/6452 ,  G01N21/6428 ,  G01N2201/062 ,  G01N2201/0642 ,  G01N2201/101 ,  H01L31/167 ,  H01L2224/48472 ,  H01L2924/10253 ,  H01L2924/3025 ,  H01L2224/48091 ,  H01L2924/00014 ,  H01L2924/00

摘要： A compact optical module for fluorescence excitation and detection and methods for using same are disclosed. An apparatus for detecting fluorescence includes a substrate base, a detector adjacent to the substrate base for determining the amount of fluorescence, an emission filter adjacent to the detector, a light source for emitting an excitation light, the light source engaging the emission filter, and a cover formed over the detector, the emission filter, and the light source.

摘要翻译： 公开了用于荧光激发和检测的紧凑型光学模块及其使用方法。 用于检测荧光的装置包括：基板，与基板基板相邻的用于确定荧光量的检测器，与检测器相邻的发射滤光器，用于发射激发光的光源，与发射滤光器接合的光源;以及 形成在检测器，发射滤光器和光源上的盖。

公开(公告)号：US20070114444A1

公开(公告)日：2007-05-24

申请号：US11451903

申请日：2006-06-12

申请人： Taylor Reid ,  Roger Taylor ,  Kenneth Zajac

发明人： Taylor Reid ,  Roger Taylor ,  Kenneth Zajac

IPC分类号： G01T1/10 ,  G01B9/10 ,  G01J1/58 ,  F21V9/16

CPC分类号： G01J3/10 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6484 ,  G01N2201/0446 ,  G01N2201/0612 ,  G01N2201/062

摘要： A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source that emits an excitation light into an illumination tube; a plurality of collection optics located around an aperture in the illumination tube for collecting fluorescence; and a detector for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light from a light source into an illumination tube; directing the excitation light to an excitation filter; illuminating a sample with the excitation light to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics located around the illumination tube.

摘要翻译： 公开了具有不同光路的用于荧光激发和检测的系统和方法。 用于检测荧光的系统包括将激发光发射到照明管中的光源; 多个收集光学器件，位于照明管中用于收集荧光的孔周围; 以及用于确定荧光量的检测器。 检测荧光的方法包括将来自光源的激发光发射到照明管中; 将激发光引导到激发滤光器; 用激发光照射样品以产生发射光; 以及使用位于照明管周围的多个收集光学器件来检测发射光的光学特性。

公开(公告)号：US20050255483A1

公开(公告)日：2005-11-17

申请号：US10852788

申请日：2004-05-25

申请人： Alan Orr ,  Roger Taylor

发明人： Alan Orr ,  Roger Taylor

IPC分类号： C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： G01N21/6428 ,  C12Q1/6851 ,  G01N21/272 ,  G01N2035/00702 ,  C12Q2527/107 ,  C12Q2537/165

摘要： The present invention is a system and method for smoothing melting curve data to help identify a specific sequence of double stranded DNA sample after performing a polymerase chain reaction. The system comprises a plurality of measurements of a fluorescence and a temperature of a dye over a temperature range to create a raw array; calculating a collapse number using a temperature resolution that facilitates the smoothing of the melting curve data, a number of data points in the raw array, and a temperature data range from the raw array; calculating a plurality of fluorescence averages and a plurality of temperature averages from the collapse number and the raw array; and generating a smooth array from the plurality of fluorescence averages and the plurality of temperature averages wherein the smooth array decreases a variability in the raw array.

摘要翻译： 本发明是一种用于平滑融化曲线数据以帮助在进行聚合酶链反应之后鉴定双链DNA样品的特定序列的系统和方法。 该系统包括在温度范围内的染料的荧光和温度的多个测量值以产生原始阵列; 使用有助于平滑熔解曲线数据的温度分辨率，原始阵列中的多个数据点和来自原始阵列的温度数据范围来计算折叠数; 从所述塌陷数和所述原始阵列计算多个荧光平均值和多个温度平均值; 以及从所述多个荧光平均值和所述多个温度平均值产生平滑阵列，其中所述平滑阵列减少所述原始阵列的变化。

公开(公告)号：US4784389A

公开(公告)日：1988-11-15

申请号：US932577

申请日：1986-11-20

申请人： Roger Taylor

发明人： Roger Taylor

IPC分类号： A63B67/20 ,  A63B69/40

CPC分类号： A63B67/20 ,  A63B21/153

摘要： A baseball batting practice device is disclosed as a bat having a recess formed at the large end to nestingly receive a spinning reel. The reel is oriented to pay-out a fishing line at a right angle to the bat axis, the fishing line is secured to a baseball and serves as a tether to retrieve the ball using the reel. With the tether payed out at a right angle to the bat axis, minimum resistance to the flight of the ball is observed and the distance traveled by the ball replicates that of an untethered ball.

摘要翻译： 棒球击球练习装置被公开为具有形成在大端处的凹部的蝙蝠，以嵌套地接收旋转卷线筒。 卷轴被定向为以与蝙蝠轴线成直角的方式支付钓鱼线，钓线被固定到棒球上，并且用作系绳以使用卷轴回收球。 随着系绳以与蝙蝠轴线成直角的方式支撑，观察到对球的飞行的最小阻力，并且球的距离重复无球的距离。

公开(公告)号：US07363168B2

公开(公告)日：2008-04-22

申请号：US10262474

申请日：2002-10-01

申请人： Roger Taylor ,  Alan Ridgeway Orr

发明人： Roger Taylor ,  Alan Ridgeway Orr

IPC分类号： G06F7/00

CPC分类号： G01N21/6428 ,  C12Q1/6851 ,  C12Q2537/165

摘要： The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithm and methods and/systems implementing the algorithm.

摘要翻译： 本发明涉及在实时PCR扩增期间开发的用于减少管对管和循环到周期变化的基线减法算法。 特别地，本发明涉及用于确定扩增的核酸产物的第一次可靠检测的阈值循环的算法。 本发明还涉及包括实现算法的算法和方法和/系统的计算机程序。

公开(公告)号：US6105726A

公开(公告)日：2000-08-22

申请号：US60760

申请日：1998-04-15

申请人： Roger Taylor ,  Lorne Walters

发明人： Roger Taylor ,  Lorne Walters

IPC分类号： B66B9/08

CPC分类号： B66B9/0838

摘要： A variable-incline ramp system guides a vehicle through the incline between a first site and a second site while at all times maintaining the vehicle in its natural horizontal orientation. The ramp system includes a separate set of rails for guiding the front and the rear wheels of the vehicle. In profile, the two sets of rails form a parallelogram having one pair of sides of a length equal to the wheelbase of the vehicle. So long as these parallelogram sides are horizontally oriented, the vehicle traveling along the rails will maintain its normal orientation because its wheelbase axis will be parallel to the horizontal sides of the parallelogram. In one embodiment suited for use in marine settings, the rails are movably connected to the first and second sites such that as the site elevations change, the parallelogram is free to skew while still remaining a parallelogram with the same length sides.

摘要翻译： 可变斜坡斜坡系统引导车辆通过第一站点和第二站点之间的倾斜，同时始终将车辆保持在其自然水平方向。 斜坡系统包括用于引导车辆的前轮和后轮的单独的导轨组。 在轮廓中，两组轨道形成平行四边形，其一对侧面的长度等于车辆的轴距。 只要这些平行四边形水平定向，沿着轨道行驶的车辆将保持其正常定向，因为其轴距轴线将平行于平行四边形的水平边。 在适合用于海洋设置的一个实施例中，轨道可移动地连接到第一和第二位置，使得当站点高度改变时，平行四边形可自由地偏斜，同时仍然保持具有相同长度侧的平行四边形。

公开(公告)号：US6073915A

公开(公告)日：2000-06-13

申请号：US255446

申请日：1999-02-23

申请人： Roger Taylor ,  Michael Coward

发明人： Roger Taylor ,  Michael Coward

IPC分类号： B66F3/12 ,  B66F3/00

CPC分类号： B66F3/12

摘要： A motorcycle jack including a support assembly (SA) for engagement with a first part (S) of a motorcycle and a lifting assembly (LA) for engagement with a second part (S) of the motorcycle, the first and second parts being on opposite sides of the motorcycle. The lifting assembly (LA) is adjustable between an initial position in which both motorcycle wheels (W) are in contact with the ground and a lifted position in which one motorcycle wheel is raised above the ground (G).

摘要翻译： 一种摩托车千斤顶，包括用于与摩托车的第一部分（S）接合的支撑组件（SA）和用于与摩托车的第二部分（S）接合的提升组件（LA），第一和第二部件相对 摩托车的两侧。 升降组件（LA）可在其中两个摩托车轮（W）与地面接触的初始位置和一个摩托车车轮在地面（G）上方升高的提升位置之间进行调节。

公开(公告)号：US20060106075A1

公开(公告)日：2006-05-18

申请号：US10522359

申请日：2003-08-08

申请人： Bernard Cuenoud ,  Robin Fairhurst ,  Roger Taylor ,  David Beattie

发明人： Bernard Cuenoud ,  Robin Fairhurst ,  Roger Taylor ,  David Beattie

IPC分类号： A61K31/428 ,  C07D277/68

CPC分类号： C07D417/12 ,  A61K31/428 ,  A61K31/454 ,  A61K31/4545 ,  A61K31/5377 ,  A61K31/55 ,  A61K45/06 ,  C07D277/68

摘要： Compounds of formula (I) in free or salt or solvate form, wherein X has the meaning indicated in the specification, are useful for treating conditions that are prevented or alleviated by activation of the β2-adrenoreceptor. Pharmaceutical compositions that contain the compounds and processes for preparing the compounds are also described.

摘要翻译： 游离或盐或溶剂合物形式的式（I）化合物，其中X具有说明书中指出的含义，可用于治疗通过β2 - 肾上腺素受体激活而预防或缓解的病症。 还描述了含有化合物的药物组合物和用于制备化合物的方法。