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    Systems and methods for effecting a physical change in a biological sample
    5.
    发明授权
    Systems and methods for effecting a physical change in a biological sample 有权
    影响生物样品物理变化的系统和方法

    公开(公告)号：US08450095B2

    公开(公告)日：2013-05-28

    申请号：US12835822

    申请日：2010-07-14

    申请人： Kyle W. Hukari ,  Jason A. A. West

    发明人： Kyle W. Hukari ,  Jason A. A. West

    IPC分类号： C12N13/00

    CPC分类号： C12N13/00 ,  C12M47/06

    摘要： The present invention relates generally to systems and methods for processing a biological sample that result in a physical change, such as reacting two molecules together to form a reaction product or for use in lysing viruses or biological cells for analysis using biological assay systems. As such, the present invention relates both to breaking apart biological species such as viruses and cells, as well as the formation of reactants from one or more reactive species. The sample has a volume in the range from about 1 microliter to 10 milliliters. The sample is processed by applying pressure, and either sonic energy or thermal energy to the sample, wherein the pressure achieved is usually at least 24 atmospheres, and the temperature of the sample is usually raised to at least 50° C.

    摘要翻译： 本发明一般涉及用于处理导致物理变化的生物样品的系统和方法，例如将两个分子一起反应以形成反应产物或用于裂解病毒或生物细胞用于使用生物测定系统进行分析。 因此，本发明涉及分解生物物种如病毒和细胞，以及从一种或多种反应性物质形成反应物。 样品的体积在约1微升至10毫升的范围内。 通过对样品施加压力和声能或热能来处理样品，其中获得的压力通常为至少24个大气压，并且样品的温度通常升高至至少50℃。

    Universal Probe Assay Methods
    6.
    发明申请
    Universal Probe Assay Methods 有权
    通用探针测定方法

    公开(公告)号：US20120115143A1

    公开(公告)日：2012-05-10

    申请号：US13280214

    申请日：2011-10-24

    申请人： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    发明人： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    摘要： Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    摘要翻译： 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并将第一检测探针与扩增产物杂交，所述第一检测探针包括与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物 。

    Systems and methods for effecting a physical change in a biological sample
    7.
    发明授权
    Systems and methods for effecting a physical change in a biological sample 有权
    影响生物样品物理变化的系统和方法

    公开(公告)号：US07781206B2

    公开(公告)日：2010-08-24

    申请号：US11402656

    申请日：2006-04-11

    申请人： Kyle W. Hukari ,  Jason A. A. West

    发明人： Kyle W. Hukari ,  Jason A. A. West

    IPC分类号： C12M1/00

    CPC分类号： C12N13/00 ,  C12M47/06

    摘要： The present invention relates generally to systems and methods for processing a biological sample that result in a physical change, such as reacting two molecules together to form a reaction product or for use in lysing viruses or biological cells for analysis using biological assay systems. As such, the present invention relates both to breaking apart biological species such as viruses and cells, as well as the formation of reactants from one or more reactive species. The sample has a volume in the range from about 1 microliter to 10 milliliters. The sample is processed by applying pressure, and either sonic energy or thermal energy to the sample, wherein the pressure achieved is usually at least 24 atmospheres, and the temperature of the sample is usually raised to at least 50° C.

    摘要翻译： 本发明一般涉及用于处理导致物理变化的生物样品的系统和方法，例如将两个分子一起反应以形成反应产物或用于裂解病毒或生物细胞用于使用生物测定系统进行分析。 因此，本发明涉及分解生物物种如病毒和细胞，以及从一种或多种反应性物质形成反应物。 样品的体积在约1微升至10毫升的范围内。 通过对样品施加压力和声能或热能来处理样品，其中获得的压力通常为至少24个大气压，并且样品的温度通常升高至至少50℃。

    Universal probe assay methods
    8.
    发明授权
    Universal probe assay methods 有权
    通用探针测定方法

    公开(公告)号：US09353406B2

    公开(公告)日：2016-05-31

    申请号：US13280214

    申请日：2011-10-24

    申请人： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    发明人： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    摘要： Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    摘要翻译： 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包含与第一探针结合序列杂交的第一区段和与第二探针结合序列杂交的第二区段，从而产生标记的扩增产物 。