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    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes
    7.
    发明授权
    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes 有权
    用于高灵敏度能量转移探针的多发色猝灭剂构建体

    公开(公告)号：US08586718B2

    公开(公告)日：2013-11-19

    申请号：US11226069

    申请日：2005-09-14

    申请人： Scott C. Benson ,  Steven M. Menchen ,  Krishna G. Upadhya

    发明人： Scott C. Benson ,  Steven M. Menchen ,  Krishna G. Upadhya

    IPC分类号： C09B62/83 ,  C09B35/037

    CPC分类号： C07C245/08 ,  C07C50/18 ,  C07D311/90 ,  C07H21/00 ,  C07H21/04 ,  C09B6/00 ,  C09B11/08 ,  C09B33/02 ,  C09B35/021 ,  C09B35/50 ,  G01N33/542

    摘要： Dark quencher constructs, termed “multi-chromophoric quenchers” are described herein that comprise at least two dark quenching moieties, which can be the same or different, linked together by at least one multivalent linking moiety. The structure of the multi-chromophoric quenchers can be varied to selectively enhance quenching within a specific range of reporter emission wavelengths. This can be accomplished by linking together, into a single molecule, two or more identical quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to quench a broader range of reporter emission wavelengths than previously possible. This can be accomplished by linking together, into a single molecule, two or more different quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to simultaneously broaden the absorption range and increase the total absorption within the absorption range. This can be done by combining the two concepts described above. In other words, multiple types of quenching moieties can be employed to increase the absorption range and a multiple number of each type of quenching moiety can be used to increase the total absorptivity within the absorption range. The multi-chromophoric quenchers can be tethered to probes for biomolecules, insoluble supports and/or fluorescent dyes for use in a wide variety of biomolecular assays.

    摘要翻译： 本文描述了称为“多发色猝灭剂”的暗猝灭剂构建体，其包含至少两个可通过至少一个多价连接部分连接在一起的相同或不同的猝灭部分。 可以改变多发色猝灭剂的结构以选择性地增强在特定范围的报道发射波长内的猝灭。 这可以通过将猝灭剂与多价连接体反应而连接在一起，形成单个分子，两个或多个相同的猝灭剂。 多发色猝灭剂的结构也可以改变以淬灭比之前可能的更广泛的报告基因发射波长。 这可以通过将猝灭剂与多价连接体反应而连接在一起成为单个分子，两个或多个不同的猝灭剂。 多色猝灭剂的结构也可以改变，以同时扩大吸收范围并增加吸收范围内的总吸收。 这可以通过组合上述两个概念来完成。 换句话说，可以使用多种类型的淬灭部分来增加吸收范围，并且可以使用多个每种类型的淬灭部分来增加吸收范围内的总吸收率。 多发色猝灭剂可以连接到用于生物分子，不溶性载体和/或荧光染料的探针，用于各种生物分子测定。

    Electron-deficient nitrogen heterocycle-substituted fluorescein dyes
    9.
    发明授权
    Electron-deficient nitrogen heterocycle-substituted fluorescein dyes 有权
    电子缺陷型氮杂环取代荧光素染料

    公开(公告)号：US06221604B1

    公开(公告)日：2001-04-24

    申请号：US09498702

    申请日：2000-02-07

    申请人： Krishna G. Upadhya ,  Steven M. Menchen ,  Weiguo Zhen

    发明人： Krishna G. Upadhya ,  Steven M. Menchen ,  Weiguo Zhen

    IPC分类号： G01N33533

    CPC分类号： C07D405/04 ,  C07D405/14 ,  C07H21/00 ,  C09B11/08 ,  Y10S436/80

    摘要： The invention provides compositions electron-deficient nitrogen heterocycle-substituted fluorescein dyes and methods in which the dyes are conjugated to substrates and used as detection labels in molecular biology experiments. The electron-deficient nitrogen heterocycles include pyridine, quinoline, pyrazine, and the like. Substrates include polynucleotides, nucleosides, nucleotides, peptides, proteins, carbohydrates, and ligands.

    摘要翻译： 本发明提供组合物电子缺乏的氮杂环取代的荧光素染料和其中染料与底物缀合并用作分子生物学实验中的检测标记的方法。 电子缺乏的氮杂环包括吡啶，喹啉，吡嗪等。 底物包括多核苷酸，核苷，核苷酸，肽，蛋白质，碳水化合物和配体。