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    4,7-dichlororhodamine dyes labeled polynucleotides
    5.
    发明授权
    4,7-dichlororhodamine dyes labeled polynucleotides 失效
    4,7-二氯罗丹明染料标记的多核苷酸

    公开(公告)号：US07550570B2

    公开(公告)日：2009-06-23

    申请号：US10788660

    申请日：2004-02-26

    申请人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Ronald J. Graham

    发明人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Ronald J. Graham

    IPC分类号： C07G3/00 ,  C07H19/04 ,  C12Q1/68

    CPC分类号： C07D401/06 ,  C07D401/14 ,  C07D405/14 ,  C07D491/147 ,  C12Q1/6869 ,  C12Q2563/107 ,  C12Q2535/101

    摘要： A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; R7-R10, R12-R16 and R18 may be hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R11 and R17 may be hydrogen, lower alkyl, lower alkene, lower alkyne, phenyl, aryl, linking group; Y1-Y4 are hydrogen, lower alkyl, or cycloalkyl, or, when taken together, Y1 and R2, Y2 and R1 Y3 and R3, and/or Y4 and R4 is propano, ethano, or substituted forms thereof; and X1-X3 taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一组用作荧光染料的4,7-二氯罗丹明化合物，其中R1-R6是氢，氟，氯，低级烷基，低级烯，低级炔，磺酸，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团时，或者当R 1和R 6一起时为苯并，或者合并时，R 4和R 5为苯并; R7-R10，R12-R16和R18可以是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸盐，砜，氨基，酰氨基，腈，低级烷氧基，连接基团; R11和R17可以是氢，低级烷基，低级烯，低级炔，苯基，芳基，连接基团; Y1-Y4是氢，低级烷基或环烷基，或者当一起为Y1和R2，Y2和R1Y3和R3，和/或Y4和R4为丙酰，乙酸或其取代形式时; 单独使用的X 1 -X 3是氢，氯，氟，低级烷基，羧酸酯，磺酸酯，羟甲基和连接基团，或其任何组合。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    4,7-DICHLORORHODAMINE DYES
    6.
    发明申请
    4,7-DICHLORORHODAMINE DYES 审中-公开
    4,7-二氯丹染色体

    公开(公告)号：US20090093623A1

    公开(公告)日：2009-04-09

    申请号：US12199441

    申请日：2008-08-27

    申请人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Ronald J. Graham

    发明人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Ronald J. Graham

    IPC分类号： C07H21/00 ,  C07H19/10

    CPC分类号： C07D401/06 ,  C07D401/14 ,  C07D405/14 ,  C07D491/147 ,  C12Q1/6869 ,  C12Q2563/107 ,  C12Q2535/101

    摘要： A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; R7-R10, R12-R16 and R18 may be hydrogen, fluorine, chlorine, lower alkyl lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R11 and R17 may be hydrogen, lower alkyl lower alkene, lower alkyne, phenyl, aryl, linking group; Y1-Y4 are hydrogen, lower alkyl or cycloalkyl, or, when taken together, Y1 and R2, Y2 and R1 Y3 and R3, and/or Y4 and R4 is propano, ethano, or substituted forms thereof, and X1-X3 taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一组用作荧光染料的4,7-二氯罗丹明化合物，其中R1-R6是氢，氟，氯，低级烷基，低级烯，低级炔，磺酸，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团时，或者当R 1和R 6一起时为苯并，或者合并时，R 4和R 5为苯并; R7-R10，R12-R16和R18可以是氢，氟，氯，低级烷基低级烯，​​低级炔，磺酸，砜，氨基，酰胺基，腈，低级烷氧基，连接基团; R11和R17可以是氢，低级烷基低级烯，​​低级炔，苯基，芳基，连接基; Y1-Y4是氢，低级烷基或环烷基，或者当Y1和R2，Y2和R1Y3和R3和/或Y4和R4分别为丙酰，乙酸或其取代形式时，分别取X1-X3 是氢，氯，氟，低级烷基，羧酸酯，磺酸酯，羟甲基和连接基团，或其任何组合。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    4,7-Dichlororhodamine dyes
    7.
    发明授权
    4,7-Dichlororhodamine dyes 失效
    4,7-二氯罗丹明染料

    公开(公告)号：US6025505A

    公开(公告)日：2000-02-15

    申请号：US38191

    申请日：1998-03-10

    申请人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    发明人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    IPC分类号： C12N15/09 ,  C07D311/80 ,  C07D311/82 ,  C07D405/12 ,  C07D491/22 ,  C07F9/6561 ,  C07H19/06 ,  C07H19/14 ,  C07H19/16 ,  C07H21/00 ,  C07H21/04 ,  C09B11/24 ,  C09B11/28 ,  C09K11/06 ,  C09K11/07 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/78 ,  G01N27/447 ,  G01N33/58 ,  C07D311/78 ,  C07D311/88

    CPC分类号： C07H21/00 ,  C09B11/24

    摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structure ##STR1## wherein R.sub.1 -R.sub.6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R.sub.1 and R.sub.6 is benzo, or, when taken together, R.sub.4 and R.sub.5 is benzo; Y.sub.1 -Y.sub.4 are hydrogen or lower alkyl, or, when taken together, Y.sub.1 and R.sub.2, Y.sub.2 and R.sub.1 Y.sub.3 and R.sub.3, or Y.sub.4 and R.sub.4 is propano, ethano, or substituted forms thereof, and X.sub.1 -X.sub.3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, --CH.sub.2 OH, and linking group. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一类用作荧光染料的4,7-二氯罗丹明化合物，其结构是其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸酯，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团，或其组合，或者当R 1和R 6一起时为苯并，或者一起使用时，R 4和R 5为苯并; Y1-Y4是氢或低级烷基，或者当Y1和R2，Y2和R1Y3和R3，或Y4和R4是它们的丙酰，乙酸或取代形式时，分别选择的X1-X3选自 由氢，氯，氟，低级烷基，羧酸酯，磺酸，-CH 2 OH和连接基组成的组。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    4,7-dichlororhodamine dyes
    8.
    发明授权
    4,7-dichlororhodamine dyes 有权
    4,7-二氯罗丹明染料

    公开(公告)号：US6017712A

    公开(公告)日：2000-01-25

    申请号：US191421

    申请日：1998-11-12

    申请人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    发明人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    IPC分类号： C07H21/00 ,  C07D311/88 ,  C12Q1/68 ,  C07H19/00

    CPC分类号： C07H21/00

    摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structure ##STR1## wherein R.sub.1 -R.sub.6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R.sub.1 and R.sub.6 is benzo, or, when taken together, R.sub.4 and R.sub.5 is benzo; Y.sub.1 -Y.sub.4 are hydrogen or lower alkyl, or, when taken together, Y.sub.1 and R.sub.2, Y.sub.2 and R.sub.1 Y.sub.3 and R.sub.3, or Y.sub.4 and R.sub.4 is propano, ethano, or substituted forms thereof; and X.sub.1 -X.sub.3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, --CH.sub.2 OH, and linking group. In another aspect, the invention includes reagents labeled with the 4,7-chlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一类用作荧光染料的4,7-二氯罗丹明化合物，其结构是其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸酯，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团，或其组合，或者当R 1和R 6一起时为苯并，或者一起使用时，R 4和R 5为苯并; Y1-Y4是氢或低级烷基，或者当Y1和R2，Y2和R1Y3和R3，或Y4和R4是丙酰基，乙酰基或取代的形式时; 和分别选择的X1-X3选自氢，氯，氟，低级烷基，羧酸酯，磺酸，-CH 2 OH和连接基团。 另一方面，本发明包括用4,7-氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。