公开(公告)号：US08173960B2

公开(公告)日：2012-05-08

申请号：US12468645

申请日：2009-05-19

申请人： Keqi Tang ,  Jason S Page ,  Ryan T Kelly ,  Richard D Smith

发明人： Keqi Tang ,  Jason S Page ,  Ryan T Kelly ,  Richard D Smith

IPC分类号： H01J49/26

CPC分类号： H01J49/165 ,  H01J49/0431 ,  H01J49/066

摘要： Systems and methods that provide up to complete transmission of ions between coupled stages with low effective ion losses. An “interfaceless” electrospray ionization system is further described that operates an electrospray at a reduced pressure such that standard electrospray sample solutions can be directly sprayed into an electrodynamic ion funnel which provides ion focusing and transmission of ions into a mass analyzer. Furthermore, chambers maintained at different pressures can allow for more optimal operating conditions for an electrospray emitter and an ion guide.

摘要翻译： 提供耦合级之间离子完全传输的系统和方法，具有低有效离子损耗。 进一步描述了一种“无接口”电喷雾离子化系统，其以减压的方式操作电喷雾，使得标准电喷雾样品溶液可以直接喷射到提供离子聚焦和离子传输到质量分析器中的电动离子漏斗中。 此外，保持在不同压力的室可以为电喷雾发射器和离子导向器提供更优化的操作条件。

公开(公告)号：US20100269573A1

公开(公告)日：2010-10-28

申请号：US12430490

申请日：2009-04-27

申请人： Ryan T. Kelly ,  Keqi Tang ,  Jason S. Page ,  Richard D. Smith

发明人： Ryan T. Kelly ,  Keqi Tang ,  Jason S. Page ,  Richard D. Smith

IPC分类号： G01N30/84 ,  H01J49/00

CPC分类号： B01L3/502784 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/0421 ,  G01N27/44743 ,  G01N30/7266 ,  H01J49/00

摘要： A transfer structure for droplet-based microfluidic analysis is characterized by a first conduit containing a first stream having at least one immiscible droplet of aqueous material and a second conduit containing a second stream comprising an aqueous fluid. The interface between the first conduit and the second conduit can define a plurality of apertures, wherein the apertures are sized to prevent exchange of the first and second streams between conduits while allowing lossless transfer of droplets from the first conduit to the second conduit through contact between the first and second streams.

摘要翻译： 用于基于液滴的微流体分析的转移结构的特征在于包含具有至少一种不混溶的水性材料的液滴的第一流的第一导管和含有含水流体的第二流的第二导管。 第一导管和第二导管之间的界面可以限定多个孔，其中孔的尺寸设定成防止第一和第二流在导管之间的交换，同时允许液滴从第一导管到第二导管的无损转移， 第一和第二个流。

公开(公告)号：US20090057551A1

公开(公告)日：2009-03-05

申请号：US11848884

申请日：2007-08-31

申请人： Keqi Tang ,  Jason S. Page ,  Ryan T. Kelly ,  Richard D. Smith

发明人： Keqi Tang ,  Jason S. Page ,  Ryan T. Kelly ,  Richard D. Smith

IPC分类号： H01J49/04 ,  B05B5/025

CPC分类号： H01J49/165 ,  H01J49/066

摘要： A system and method are disclosed that provide up to complete transmission of ions between coupled stages with low effective ion losses. A novel “interfaceless” electrospray ionization system is further described that operates the electrospray at a reduced pressure such that standard electrospray sample solutions can be directly sprayed into an electrodynamic ion funnel which provides ion focusing and transmission of ions into a mass analyzer.

摘要翻译： 公开了一种系统和方法，其提供在具有低有效离子损耗的耦合级之间的离子的完全传输。 进一步描述了一种新颖的“无接触”电喷雾离子化系统，其在减压下操作电喷雾，使得标准电喷雾样品溶液可以直接喷射到提供离子聚焦并将离子传输到质量分析器中的电动离子漏斗中。

公开(公告)号：US08061187B2

公开(公告)日：2011-11-22

申请号：US12430490

申请日：2009-04-27

申请人： Ryan T Kelly ,  Keqi Tang ,  Jason S Page ,  Richard D Smith

发明人： Ryan T Kelly ,  Keqi Tang ,  Jason S Page ,  Richard D Smith

IPC分类号： G01N13/00

CPC分类号： B01L3/502784 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/0421 ,  G01N27/44743 ,  G01N30/7266 ,  H01J49/00

摘要： A transfer structure for droplet-based microfluidic analysis is characterized by a first conduit containing a first stream having at least one immiscible droplet of aqueous material and a second conduit containing a second stream comprising an aqueous fluid. The interface between the first conduit and the second conduit can define a plurality of apertures, wherein the apertures are sized to prevent exchange of the first and second streams between conduits while allowing lossless transfer of droplets from the first conduit to the second conduit through contact between the first and second streams.

摘要翻译： 用于基于液滴的微流体分析的转移结构的特征在于包含具有至少一种不混溶的水性材料的液滴的第一流的第一导管和含有含水流体的第二流的第二导管。 第一导管和第二导管之间的界面可以限定多个孔，其中孔的尺寸设定成防止第一和第二流在导管之间的交换，同时允许液滴从第一导管到第二导管的无损转移， 第一和第二个流。

公开(公告)号：US07671344B2

公开(公告)日：2010-03-02

申请号：US11848884

申请日：2007-08-31

申请人： Keqi Tang ,  Jason S. Page ,  Ryan T. Kelly ,  Richard D. Smith

发明人： Keqi Tang ,  Jason S. Page ,  Ryan T. Kelly ,  Richard D. Smith

IPC分类号： H01J27/00

CPC分类号： H01J49/165 ,  H01J49/066

摘要： A system and method are disclosed that provide up to complete transmission of ions between coupled stages with low effective ion losses. A novel “interfaceless” electrospray ionization system is further described that operates the electrospray at a reduced pressure such that standard electrospray sample solutions can be directly sprayed into an electrodynamic ion funnel which provides ion focusing and transmission of ions into a mass analyzer.

摘要翻译： 公开了一种系统和方法，其提供在具有低有效离子损耗的耦合级之间的离子的完全传输。 进一步描述了一种新颖的“无接触”电喷雾离子化系统，其在减压下操作电喷雾，使得标准电喷雾样品溶液可以直接喷射到提供离子聚焦并将离子传输到质量分析器中的电动离子漏斗中。

公开(公告)号：US20090242755A1

公开(公告)日：2009-10-01

申请号：US12468645

申请日：2009-05-19

申请人： Keqi Tang ,  Jason S. Page ,  Ryan T. Kelly ,  Richard D. Smith

发明人： Keqi Tang ,  Jason S. Page ,  Ryan T. Kelly ,  Richard D. Smith

IPC分类号： H01J49/00

CPC分类号： H01J49/165 ,  H01J49/0431 ,  H01J49/066

摘要： Systems and methods that provide up to complete transmission of ions between coupled stages with low effective ion losses. An “interfaceless” electrospray ionization system is further described that operates an electrospray at a reduced pressure such that standard electrospray sample solutions can be directly sprayed into an electrodynamic ion funnel which provides ion focusing and transmission of ions into a mass analyzer. Furthermore, chambers maintained at different pressures can allow for more optimal operating conditions for an electrospray emitter and an ion guide.

摘要翻译： 提供耦合级之间离子完全传输的系统和方法，具有低有效离子损耗。 进一步描述了一种“无接口”电喷雾离子化系统，其以减压的方式操作电喷雾，使得标准电喷雾样品溶液可以直接喷射到提供离子聚焦和离子传输到质量分析器中的电动离子漏斗中。 此外，保持在不同压力的室可以为电喷雾发射器和离子导向器提供更优化的操作条件。

公开(公告)号：US07491341B2

公开(公告)日：2009-02-17

申请号：US11394840

申请日：2006-03-31

申请人： Ryan T. Kelly ,  Jason S. Page ,  Keqi Tang ,  Richard D. Smith

发明人： Ryan T. Kelly ,  Jason S. Page ,  Keqi Tang ,  Richard D. Smith

IPC分类号： C03C25/68 ,  B44C1/22

CPC分类号： H01J49/167

摘要： Methods of forming electrospray ionization emitter tips are disclosed herein. In one embodiment, an end portion of a capillary tube can be immersed into an etchant, wherein the etchant forms a concave meniscus on the outer surface of the capillary. Variable etching rates in the meniscus can cause an external taper to form. While etching the outer surface of the capillary wall, a fluid can be flowed through the interior of the capillary tube. Etching continues until the immersed portion of the capillary tube is completely etched away.

摘要翻译： 本文公开了形成电喷雾电离发射器尖端的方法。 在一个实施例中，毛细管的端部可以浸入蚀刻剂中，其中蚀刻剂在毛细管的外表面上形成凹弯月面。 弯液面中的可变蚀刻速率可能导致形成外部锥度。 在蚀刻毛细管壁的外表面时，流体可以流过毛细管的内部。 继续蚀刻直到毛细管的浸入部分被完全蚀刻掉。

公开(公告)号：US20090108194A1

公开(公告)日：2009-04-30

申请号：US11251529

申请日：2005-09-30

申请人： Jason S. Page ,  Keqi Tang ,  Richard D. Smith

发明人： Jason S. Page ,  Keqi Tang ,  Richard D. Smith

IPC分类号： B01D59/44

CPC分类号： H01J49/066 ,  G01N27/622

摘要： An adjustable, low mass-to-charge (m/z) filter is disclosed employing electrospray ionization to block ions associated with unwanted low m/z species from entering the mass spectrometer and contributing their space charge to down-stream ion accumulation steps. The low-mass filter is made by using an adjustable potential energy barrier from the conductance limiting terminal electrode of an electrodynamic ion funnel, which prohibits species with higher ion mobilities from being transmitted. The filter provides a linear voltage adjustment of low-mass filtering from m/z values from about 50 to about 500. Mass filtering above m/z 500 can also be performed; however, higher m/z species are attenuated. The mass filter was evaluated with a liquid chromatography-mass spectrometry analysis of an albumin tryptic digest and resulted in the ability to block low-mass, “background” ions which account for 40-70% of the total ion current from the ESI source during peak elution.

摘要翻译： 公开了一种可调节的低质量 - 电荷（m / z）滤波器，其使用电喷雾电离来阻挡与不想要的低m / z物质相关的离子进入质谱仪并且为下游离子累积步骤贡献其空间电荷。 低质量过滤器通过使用来自电动离子漏斗的电导限制端子电极的可调势势垒来制造，其阻止具有较高离子迁移率的物质被传输。 滤波器提供从大约50到大约500的m / z值的低质量滤波的线性电压调节。也可以执行m / z 500以上的质量过滤; 然而，更高的m / z物种减弱。 通过对白蛋白胰蛋白酶消化物的液相色谱 - 质谱分析来评估质量过滤器，并且导致阻断低质量“背景”离子的能力，其占离子源的总离子电流的40-70％ 峰洗脱。

公开(公告)号：US20120074066A1

公开(公告)日：2012-03-29

申请号：US12889108

申请日：2010-09-23

申请人： Xuefei Sun ,  Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

发明人： Xuefei Sun ,  Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

IPC分类号： G01N27/416 ,  B01D15/08 ,  G01N30/02 ,  B01D17/06

CPC分类号： G01N27/44743 ,  G01N27/44791 ,  G01N30/461

摘要： Microchip capillary electrophoresis (CE) utilizing a sample injector based on a mechanical valve rather than electrokinetic injection can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. In one instance CE electrodes continuously apply an electric field for CE separation along a separation channel. A sample channel is connected to the separation channel at an intersection and has a sample pressure that is greater than that which is present in the separation channel near the intersection. The sample channel does not have electrodes that apply voltages for electrokinetic injection. A sample injector in the sample channel or at the intersection comprises a mechanical valve to control sample injection from the sample channel to the separation channel.

摘要翻译： 使用基于机械阀而不是电动注射的样品注射器的微芯片毛细管电泳（CE）可以提供改进的样品注射，增强的能力，并且可以消除改变分离通道中的电场以诱导注射样品的需要。 在一个实例中，CE电极沿着分离通道连续地施加用于CE分离的电场。 样品通道在交叉口处连接到分离通道，并且具有大于在交叉口附近的分离通道中存在的样品压力的样品压力。 样品通道不具有施加电动注射电压的电极。 样品通道或交叉口处的样品注射器包括一个机械阀，用于控制从样品通道到分离通道的样品注射。

公开(公告)号：US20090230296A1

公开(公告)日：2009-09-17

申请号：US12046207

申请日：2008-03-11

申请人： Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

发明人： Ryan T. Kelly ,  Keqi Tang ,  Richard D. Smith

IPC分类号： B01D59/44

CPC分类号： H01J49/167

摘要： Electrospray ionization emitter arrays, as well as methods for forming electrosprays, are described. The arrays are characterized by a radial configuration of three or more nano-electrospray ionization emitters without an extractor electrode. The methods are characterized by distributing fluid flow of the liquid sample among three or more nano-electrospray ionization emitters, forming an electrospray at outlets of the emitters without utilizing an extractor electrode, and directing the electrosprays into an entrance to a mass spectrometry device. Each of the nano-electrospray ionization emitters can have a discrete channel for fluid flow. The nano-electrospray ionization emitters are circularly arranged such that each is shielded substantially equally from an electrospray-inducing electric field.

摘要翻译： 描述了电喷雾电离发射器阵列，以及用于形成电喷雾的方法。 阵列的特征在于没有提取器电极的三个或更多个纳米电喷雾电离发射器的径向构型。 该方法的特征在于在三个或更多个纳米电喷雾电离发射器之间分配液体样品的流体流动，在不使用提取器电极的情况下在发射器的出口处形成电喷雾，并将电喷雾引导到质谱装置的入口。 每个纳米电喷雾电离发射器可以具有用于流体流动的离散通道。 纳米电喷雾电离发射器是圆形布置的，使得每个发射器基本上等同于电喷雾诱导电场屏蔽。