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公开(公告)号：US3993543A
公开(公告)日：1976-11-23
申请号：US593291
申请日：1975-07-07
申请人： Ryosuke Uchio , Kenji Kikuchi , Hitoshi Enei , Yoshio Hirose
发明人： Ryosuke Uchio , Kenji Kikuchi , Hitoshi Enei , Yoshio Hirose
CPC分类号： C12P7/40 , Y10S435/923
摘要： Improved method for producing pyruvic acid by fermentation of pyruvic acid producing mutants of the strain Candida lipolytica which require methionine and thiamine for growth.
摘要翻译： 通过发酵产生丙酮酸的方法，其用于产生需要甲硫氨酸和硫胺素生长的解脂耶氏酵母菌株的产生丙酮酸的突变体。
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公开(公告)号：US3960660A
公开(公告)日：1976-06-01
申请号：US562402
申请日：1975-03-27
申请人： Hitoshi Enei , Katsuaki Sato , Yoshio Hirose
发明人： Hitoshi Enei , Katsuaki Sato , Yoshio Hirose
IPC分类号： C07H19/167 , C12P19/40 , C12R1/07 , C12D13/06
CPC分类号： C12P19/40 , Y10S435/839
摘要： Production of guanosine by fermentation with a Bacillus mutant requiring adenine for growth and resistant to psicofuranine or decoyinine.
摘要翻译： 通过用芽孢杆菌突变体发酵生产鸟苷，需要腺嘌呤生长并且对丝心蛋白或去甲肾上腺素具有抗性。
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公开(公告)号：US3969188A
公开(公告)日：1976-07-13
申请号：US517631
申请日：1974-10-24
申请人： Hitoshi Enei , Katsuaki Sato , Yasuo Anzai , Yoshio Hirose
发明人： Hitoshi Enei , Katsuaki Sato , Yasuo Anzai , Yoshio Hirose
CPC分类号： C07H19/16 , C12P19/40 , Y10S435/839
摘要： Method for producing guanosine by culturing a guanosine-producing mutant of the genus Bacillus which requires adenine for growth, and is resistant to at least one sulfa drug.
摘要翻译： 通过培养需要腺嘌呤生长的芽孢杆菌属的产生鸟苷的突变体并且对至少一种磺胺药具有抗性来制备鸟苷的方法。
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公开(公告)号：US3960661A
公开(公告)日：1976-06-01
申请号：US562401
申请日：1975-03-27
申请人： Hitoshi Enei , Katsuaki Sato , Morikatsu Ishii , Yoshio Hirose
发明人： Hitoshi Enei , Katsuaki Sato , Morikatsu Ishii , Yoshio Hirose
CPC分类号： C12P19/40 , Y10S435/839
摘要： Mutant strains of Bacillus subtilis which require adenine for growth and are resistant to at least one sulfa drug produce inosine in culture medium.
摘要翻译： 需要腺嘌呤生长并对至少一种磺胺药物在培养基中产生肌苷的抗性的枯草芽孢杆菌突变菌株。
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公开(公告)号：US4591562A
公开(公告)日：1986-05-27
申请号：US519714
申请日：1983-08-02
IPC分类号： C12N15/52 , C12P13/22 , C07H21/04 , C12N1/00 , C12N1/20 , C12N15/00 , C12P19/34 , C12P21/00 , C12P21/02 , C12R1/125
CPC分类号： C12N15/52 , C12P13/222 , Y10S435/839
摘要： A method for producing L-phenylalanine by fermentation which comprises aerobically culturing an L-phenylalanine-producing microorganism in an aqueous culture medium and recovering the L-phenylalanine accumulated in the culture medium, the L-phenylalanine-producing microorganism having been constructed by incorporating a recombinant plasmid DNA, into which a DNA fragment controlling resistance to a phenylalanine antagoinst, obtained from a chromosomal DNA of a mutant of the genus Bacillus resistant to the phenylalanine antagonist, has been inserted, into a recipient strain of the genus Bacillus.
摘要翻译： 通过发酵生产L-苯丙氨酸的方法，其包括在水性培养基中有氧培养产生L-苯丙氨酸的微生物并回收在培养基中积累的L-苯丙氨酸，所述L-苯丙氨酸生产微生物通过掺入 将从对苯丙氨酸拮抗剂耐药的芽孢杆菌的突变体的染色体DNA获得的控制对苯丙氨酸antagoinst的抗性的DNA片段插入其中的重组质粒DNA插入到芽孢杆菌属的受体菌株中。
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公开(公告)号：US4504581A
公开(公告)日：1985-03-12
申请号：US448792
申请日：1982-12-10
CPC分类号： C12R1/125 , C12N15/52 , C12P13/24 , Y10S435/839
摘要： L-Histidine producing microorganisms which have been constructed by introducing a recombinant plasmid DNA inserted on a chromosomal DNA fragment into a recipient strain of the genus Bacillus. These microorganisms are employed to produce L-histidine in higher than normal yields. The recombinant plasmid DNA inserted is obtained from a donor mutant strain of Bacillus subtilis which is resistant to certain L-histidine antagonists. The resistant plasmid confers the properties of the mutant strain upon the recipient Bacillus subtilis to make its high yield of product even higher.
摘要翻译： 通过将插入染色体DNA片段的重组质粒DNA引入芽孢杆菌属的受体菌株而构建的L-组氨酸生产微生物。 这些微生物用于以高于正常产率产生L-组氨酸。 插入的重组质粒DNA从对某些L-组氨酸拮抗剂具有抗性的枯草芽孢杆菌供体突变菌株获得。 抗性质粒赋予受体枯草芽孢杆菌上的突变菌株的性质，使其高产率的产物更高。
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公开(公告)号：US5272067A
公开(公告)日：1993-12-21
申请号：US830530
申请日：1992-02-04
CPC分类号： C12P13/14 , Y10S435/84 , Y10S435/843
摘要： A process for the production of L-glutamic acid comprising growing microorganisms belonging to the genera Brevibacterium and Corynebacterium that are resisted to prumycin.
摘要翻译： 一种生产L-谷氨酸的方法，包括生长属于短杆菌属和棒状杆菌属的微生物，其抗嘌呤霉素。
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8.发明授权Coryneform bacteria carrying recombinant DNA and a process for producing aromatic amino acids using said bacteria 失效携带重组DNA的棒状细菌和使用所述细菌生产芳香族氨基酸的方法
公开(公告)号：US4968609A
公开(公告)日：1990-11-06
申请号：US316961
申请日：1989-02-28
申请人： Hisao Ito , Katsuaki Sato , Kazuhiko Matsui , Konosuke Sano , Shigeru Nakamori , Takashi Tanaka , Hitoshi Enei
发明人： Hisao Ito , Katsuaki Sato , Kazuhiko Matsui , Konosuke Sano , Shigeru Nakamori , Takashi Tanaka , Hitoshi Enei
CPC分类号： C12P13/22 , C12N15/52 , Y10S435/84 , Y10S435/843
摘要： The present invention relates to a process for producing an aromatic amino acid which comprises culturing a Coryneform bacterium carrying a recombinant DNA constructed by connecting (1) a gene capable of being expressed in a Coryneform bacterial cell and coding for 3-deoxy-D-arabino-heptulonic acid-7-phosphate synthetase with (2) a plasmid vector capable of propagating in a Coryneform bacterial cell.
摘要翻译： 本发明涉及一种芳香族氨基酸的制造方法，其包括培养携带重组DNA的棒状杆菌属细菌，所述重组DNA通过连接（1）能够在棒状细菌细胞中表达的基因并编码3-脱氧-D-阿拉伯糖 - 庚酸7-磷酸合成酶，（2）能在棒状细菌细胞中繁殖的质粒载体。
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公开(公告)号：US4588687A
公开(公告)日：1986-05-13
申请号：US444151
申请日：1982-11-24
CPC分类号： C12P13/227 , C12N15/52 , Y10S435/839
摘要： A method for producing L-tryptophan by fermentation in high yields employing a microorganism of the genus Bacillus, which has been constructed by a gene splicing technique by incorporating a recombinant plasmid DNA containing an inserted DNA containing fragment controlling resistance to tryptophan-antagonists into a recipient strain of the genus Bacillus. The incorporated DNA fragment is a mutant of the genus Bacillus which was obtained from resistant to tryptophan-antagonists.
摘要翻译： 通过使用芽孢杆菌属的微生物以高产率生产L-色氨酸的方法，其通过基因剪接技术构建，通过将含有插入的DNA的含有控制抗性的片段的重组质粒DNA掺入到色氨酸拮抗剂中， 芽孢杆菌属菌株。 掺入的DNA片段是从对色氨酸 - 拮抗剂的抗性获得的芽孢杆菌属的突变体。
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公开(公告)号：US4560652A
公开(公告)日：1985-12-24
申请号：US444172
申请日：1982-11-24
申请人： Osamu Kurahashi , Masahiro Kamada , Hitoshi Enei
发明人： Osamu Kurahashi , Masahiro Kamada , Hitoshi Enei
CPC分类号： C12P13/227 , Y10S435/839
摘要： A process for economically producing L-tryptophan which involves culturing aerobically in a culture medium a mutant of the genus Bacillus which is resistant to azaserine and a tryptophan analogue and recovering the L-tryptophan produced which accumulates in the culture medium.
摘要翻译： 一种用于经济地生产L-色氨酸的方法，其涉及在培养基中有氧地培养耐碱性丝氨酸和色氨酸类似物的芽孢杆菌属的突变体，并回收在培养基中积聚的L-色氨酸。
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