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    Method for producing L-phenylalanine by fermentation
    1.
    发明授权
    Method for producing L-phenylalanine by fermentation 失效
    通过发酵生产L-苯丙氨酸的方法

    公开(公告)号：US4591562A

    公开(公告)日：1986-05-27

    申请号：US519714

    申请日：1983-08-02

    申请人： Osamu Kurahashi ,  Takayasu Tsuchida ,  Hiroki Kawashima ,  Hitoshi Enei

    发明人： Osamu Kurahashi ,  Takayasu Tsuchida ,  Hiroki Kawashima ,  Hitoshi Enei

    IPC分类号： C12N15/52 ,  C12P13/22 ,  C07H21/04 ,  C12N1/00 ,  C12N1/20 ,  C12N15/00 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02 ,  C12R1/125

    CPC分类号： C12N15/52 ,  C12P13/222 ,  Y10S435/839

    摘要： A method for producing L-phenylalanine by fermentation which comprises aerobically culturing an L-phenylalanine-producing microorganism in an aqueous culture medium and recovering the L-phenylalanine accumulated in the culture medium, the L-phenylalanine-producing microorganism having been constructed by incorporating a recombinant plasmid DNA, into which a DNA fragment controlling resistance to a phenylalanine antagoinst, obtained from a chromosomal DNA of a mutant of the genus Bacillus resistant to the phenylalanine antagonist, has been inserted, into a recipient strain of the genus Bacillus.

    摘要翻译： 通过发酵生产L-苯丙氨酸的方法，其包括在水性培养基中有氧培养产生L-苯丙氨酸的微生物并回收在培养基中积累的L-苯丙氨酸，所述L-苯丙氨酸生产微生物通过掺入 将从对苯丙氨酸拮抗剂耐药的芽孢杆菌的突变体的染色体DNA获得的控制对苯丙氨酸antagoinst的抗性的DNA片段插入其中的重组质粒DNA插入到芽孢杆菌属的受体菌株中。

    METHOD FOR PRODUCING L-METHIONINE BY FERMENTATION
    8.
    发明申请
    METHOD FOR PRODUCING L-METHIONINE BY FERMENTATION 有权
    通过发酵生产L-甲硫氨酸的方法

    公开(公告)号：US20100041108A1

    公开(公告)日：2010-02-18

    申请号：US12555290

    申请日：2009-09-08

    申请人： Yoshihiro USUDA ,  Osamu Kurahashi

    发明人： Yoshihiro USUDA ,  Osamu Kurahashi

    IPC分类号： C12P13/12

    CPC分类号： C12P13/12 ,  C12N9/0006 ,  C12N9/1018 ,  C12N9/1085 ,  C12N9/1205

    摘要： The present invention provides a method for producing L-methionine by culturing a microorganism in a medium to produce and accumulate L-methionine in the medium, and collecting the L-methionine from the medium, where the microorganism is deficient in a repressor of L-methionine biosynthesis system and has L-methionine productivity.

    摘要翻译： 本发明提供一种通过在培养基中培养微生物来生产L-甲硫氨酸的方法，以在培养基中产生和积累L-甲硫氨酸，并从培养基中收集L-甲硫氨酸，其中微生物缺乏L- 甲硫氨酸生物合成系统，具有L-甲硫氨酸生产力。

    Method for producing purine nucleoside by fermentation
    9.
    发明授权
    Method for producing purine nucleoside by fermentation 失效
    通过发酵生产嘌呤核苷的方法

    公开(公告)号：US07435560B1

    公开(公告)日：2008-10-14

    申请号：US09462472

    申请日：1998-07-17

    申请人： Hiroshi Matsui ,  Hisashi Kawasaki ,  Megumi Shimaoka ,  Yasuhiro Takenaka ,  Osamu Kurahashi

    发明人： Hiroshi Matsui ,  Hisashi Kawasaki ,  Megumi Shimaoka ,  Yasuhiro Takenaka ,  Osamu Kurahashi

    IPC分类号： C12P19/38 ,  C12N9/10 ,  C12N9/12 ,  C12N1/00 ,  C12N1/20

    CPC分类号： C12N9/1077 ,  C12P19/38 ,  C12P19/40 ,  C12R1/19

    摘要： A microorganism which has a gene encoding an enzyme in which feedback inhibition is desensitized by substitution of one or two amino acids in PRPP amidotransferase encoded by purF of Escherichia coli, a gene encoding a protein which is an inactivated repressor of purine nucleotide biosynthesis encoded by purR, a gene encoding an enzyme which is inactivated purine nucleoside phosphorylase encoded by deoD, a gene encoding an enzyme which is inactivated succinyl-AMP synthase encoded by purA, a gene encoding an enzyme which is inactivated 6-phosphogluconate dehydrase encoded by edd, a gene encoding an enzyme which is inactivated phosphoglucose isomerase encoded by pgi and like is bred and a purine nucleoside is produced by culturing the microorganism.

    摘要翻译： 具有编码酶的基因的微生物，其中通过用大肠杆菌的purF编码的PRPP酰胺转移酶中的一个或两个氨基酸取代反馈抑制而脱敏，该基因是编码由purR编码的嘌呤核苷酸生物合成的灭活性阻遏物的蛋白质 编码由deoD编码的失活的嘌呤核苷磷酸化酶的酶的基因，编码由purA编码的失活的琥珀酰-PAMP合酶的酶的基因，编码由edd编码的6-磷酸葡萄糖酸脱水酶的酶的基因， 编码由pgi等编码的灭活的磷酸葡萄糖异构酶的酶，并通过培养微生物产生嘌呤核苷。

    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine
    10.
    发明授权
    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
    棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法

    公开(公告)号：US07297521B2

    公开(公告)日：2007-11-20

    申请号：US11011701

    申请日：2004-12-15

    申请人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    发明人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    IPC分类号： C12P13/10

    CPC分类号： C12N9/93 ,  C12P13/10

    摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

    摘要翻译： 编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽 序列表所示的SEQ ID NO：2中的50〜393的酸值，（b）至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代， 一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质，其具有氨基酸序列SEQ ID NO：3的氨基甲酰磷酸合成酶的大亚基，（c ）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，（d）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，包括取代，缺失，插入，添加， 或反转一个或几个氨基酸，并可构成 具有氨基酸 - 磷酸合成酶活性的蛋白质，其具有SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。