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公开(公告)号：US4504581A
公开(公告)日：1985-03-12
申请号：US448792
申请日：1982-12-10
CPC分类号： C12R1/125 , C12N15/52 , C12P13/24 , Y10S435/839
摘要： L-Histidine producing microorganisms which have been constructed by introducing a recombinant plasmid DNA inserted on a chromosomal DNA fragment into a recipient strain of the genus Bacillus. These microorganisms are employed to produce L-histidine in higher than normal yields. The recombinant plasmid DNA inserted is obtained from a donor mutant strain of Bacillus subtilis which is resistant to certain L-histidine antagonists. The resistant plasmid confers the properties of the mutant strain upon the recipient Bacillus subtilis to make its high yield of product even higher.
摘要翻译： 通过将插入染色体DNA片段的重组质粒DNA引入芽孢杆菌属的受体菌株而构建的L-组氨酸生产微生物。 这些微生物用于以高于正常产率产生L-组氨酸。 插入的重组质粒DNA从对某些L-组氨酸拮抗剂具有抗性的枯草芽孢杆菌供体突变菌株获得。 抗性质粒赋予受体枯草芽孢杆菌上的突变菌株的性质，使其高产率的产物更高。
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公开(公告)号：US4588687A
公开(公告)日：1986-05-13
申请号：US444151
申请日：1982-11-24
CPC分类号： C12P13/227 , C12N15/52 , Y10S435/839
摘要： A method for producing L-tryptophan by fermentation in high yields employing a microorganism of the genus Bacillus, which has been constructed by a gene splicing technique by incorporating a recombinant plasmid DNA containing an inserted DNA containing fragment controlling resistance to tryptophan-antagonists into a recipient strain of the genus Bacillus. The incorporated DNA fragment is a mutant of the genus Bacillus which was obtained from resistant to tryptophan-antagonists.
摘要翻译： 通过使用芽孢杆菌属的微生物以高产率生产L-色氨酸的方法，其通过基因剪接技术构建，通过将含有插入的DNA的含有控制抗性的片段的重组质粒DNA掺入到色氨酸拮抗剂中， 芽孢杆菌属菌株。 掺入的DNA片段是从对色氨酸 - 拮抗剂的抗性获得的芽孢杆菌属的突变体。
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公开(公告)号：US4591562A
公开(公告)日：1986-05-27
申请号：US519714
申请日：1983-08-02
IPC分类号： C12N15/52 , C12P13/22 , C07H21/04 , C12N1/00 , C12N1/20 , C12N15/00 , C12P19/34 , C12P21/00 , C12P21/02 , C12R1/125
CPC分类号： C12N15/52 , C12P13/222 , Y10S435/839
摘要： A method for producing L-phenylalanine by fermentation which comprises aerobically culturing an L-phenylalanine-producing microorganism in an aqueous culture medium and recovering the L-phenylalanine accumulated in the culture medium, the L-phenylalanine-producing microorganism having been constructed by incorporating a recombinant plasmid DNA, into which a DNA fragment controlling resistance to a phenylalanine antagoinst, obtained from a chromosomal DNA of a mutant of the genus Bacillus resistant to the phenylalanine antagonist, has been inserted, into a recipient strain of the genus Bacillus.
摘要翻译： 通过发酵生产L-苯丙氨酸的方法，其包括在水性培养基中有氧培养产生L-苯丙氨酸的微生物并回收在培养基中积累的L-苯丙氨酸，所述L-苯丙氨酸生产微生物通过掺入 将从对苯丙氨酸拮抗剂耐药的芽孢杆菌的突变体的染色体DNA获得的控制对苯丙氨酸antagoinst的抗性的DNA片段插入其中的重组质粒DNA插入到芽孢杆菌属的受体菌株中。
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公开(公告)号：US5272067A
公开(公告)日：1993-12-21
申请号：US830530
申请日：1992-02-04
CPC分类号： C12P13/14 , Y10S435/84 , Y10S435/843
摘要： A process for the production of L-glutamic acid comprising growing microorganisms belonging to the genera Brevibacterium and Corynebacterium that are resisted to prumycin.
摘要翻译： 一种生产L-谷氨酸的方法，包括生长属于短杆菌属和棒状杆菌属的微生物，其抗嘌呤霉素。
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公开(公告)号：US4347318A
公开(公告)日：1982-08-31
申请号：US136475
申请日：1980-04-02
申请人： Kiyoshi Miwa , Takayasu Tsuchida , Osamu Kurahashi , Shigeru Nakamori , Konosuke Sano , Haruo Momose
发明人： Kiyoshi Miwa , Takayasu Tsuchida , Osamu Kurahashi , Shigeru Nakamori , Konosuke Sano , Haruo Momose
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N15/00 , C12N15/52 , C12P13/08 , C12P19/34 , C12R1/19 , C12N1/20
CPC分类号： C12N15/52 , C12P13/08 , Y10S435/849
摘要： L-threonine is produced by incorporating into a recipient microorganism of the genus Escherichia which does not require L-threonine for growth, a plasmid, in which a deoxyribonucleic acid fragment which possesses genetic information relating to L-threonine synthesis obtained from a mutant resistant to .alpha.-amino-.beta.-hydroxy valeric acid of the genus Escherichia, has been inserted.
摘要翻译： L-苏氨酸是通过将不需要L-苏氨酸生长的埃希氏杆菌属的受体微生物中的一种质粒制成的，其中具有与由抗突变型突变体抵抗的突变体获得的L-苏氨酸合成有关的遗传信息的脱氧核糖核酸片段 已经插入了埃希氏杆菌属的α-氨基-β-羟基戊酸。
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公开(公告)号：US4778762A
公开(公告)日：1988-10-18
申请号：US434853
申请日：1982-10-18
申请人： Kiyoshi Miwa , Mahito Terabe , Takayasu Tsuchida , Masaaki Ishida , Shigeru Nakamori , Konosuke Sano , Haruo Momose
发明人： Kiyoshi Miwa , Mahito Terabe , Takayasu Tsuchida , Masaaki Ishida , Shigeru Nakamori , Konosuke Sano , Haruo Momose
IPC分类号： C12P13/08 , C12P13/14 , C12N15/00 , C12N1/20 , C12P19/34 , C12P21/00 , C12P21/02 , C12R1/13 , C12R1/15
CPC分类号： C12R1/13 , C12P13/08 , C12P13/14 , C12R1/15 , Y10S435/84 , Y10S435/843
摘要： A substantially pure plasmid which is characterized by a molecular weight of 3.0.+-.0.1 megadalton and the restriction endonuclease-cleavage map shown in the Figure. This plasmid is capable of propagating in Corynebacteria and due to its size is useful as a vector for the cloning of exogenous genes.
摘要翻译： 一种基本上纯的质粒，其特征在于分子量为3.0 +/- 0.1兆道尔顿和限制性内切核酸酶切割图，如图所示。 该质粒能够在棒状杆菌中繁殖，并且由于其大小可用作克隆外源基因的载体。
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公开(公告)号：US4442208A
公开(公告)日：1984-04-10
申请号：US392145
申请日：1982-06-25
IPC分类号： C12N15/09 , C12N1/21 , C12N15/52 , C12N15/77 , C12P13/06 , C12R1/13 , C12R1/15 , C12N1/20 , C12N15/00
摘要： L-isoleucine is produced by a fermentation process comprising aerobically culturing in an aqueous culture medium an L-isoleucine producing microorganism obtained by isolating a transformed strain resistant to .alpha.-amino-.beta.-hydroxy valeric acid prepared by incorporating into a recipient strain of the genus Brevibacterium orCorynebacterium, which is sensitive to .alpha.-amino-.beta.-hydroxy valeric acid, a plasmid DNA obtained from a microorganism of the genus Brevibacterium or Corynebacterium into which has been inserted a fragment of chromosomal DNA derived from a DNA-donor strain of the genus Brevibacterium or Corynebacterium which is resistant to .alpha.-amino-.beta.-hydroxy valeric acid; and recovering L-isoleucine which accumulates in the resulting culture liquid.
摘要翻译： 通过发酵方法产生L-异亮氨酸，所述发酵方法包括在水性培养基中有氧培养产生的L-异亮氨酸微生物，其通过分离通过掺入到所述属的受体菌株中而制备的对α-氨基-β-羟基戊酸有抗性的转化菌株 对α-氨基-β-羟基戊酸敏感的短杆菌或棒状杆菌，从短杆菌属或棒状杆菌属的微生物获得的质粒DNA，其中插入了源自该属的DNA供体菌株的染色体DNA片段 对α-氨基-β-羟基戊酸有抗性的短杆菌属或棒状杆菌; 并回收在所得培养液中积聚的L-异亮氨酸。
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公开(公告)号：US4427773A
公开(公告)日：1984-01-24
申请号：US255169
申请日：1981-04-17
CPC分类号： C12R1/13 , C12N15/52 , C12N15/77 , C12P13/14 , C12R1/15 , Y10S435/84 , Y10S435/843
摘要： An L-glutamic acid producing microorganism which is constructed by incorporation into a recipient strain of the genus Brevibacterium or Corynebacterium of a hybrid plasmid having inserted therein a DNA fragment with genetic information related to L-glutamic acid production which is derived from a donor strain of the genus Brevibacterium or Corynebacterium, is useful for the production of high levels of L-glutamic acid by fermentation.
摘要翻译： 通过掺入具有与L-谷氨酸生产相关的遗传信息的DNA片段的杂交质粒的杂交质粒的并入短杆菌属或棒状杆菌属的受体菌株而构建的L-谷氨酸微生物，所述遗传信息衍生自与供体菌株 短杆菌属或棒状杆菌属，可用于通过发酵生产高水平的L-谷氨酸。
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公开(公告)号：US4391907A
公开(公告)日：1983-07-05
申请号：US212123
申请日：1980-12-02
CPC分类号： C12N15/52 , C12P13/08 , Y10S435/848 , Y10S435/849
摘要： An L-valine-producing microorganism which is constructed by incorporation into a host strain of the genus Escherichia of a hybrid plasmid having inserted therein a DNA fragment with genetic information related to L-valine production which is derived from a donor strain of the genus Escherichia which is resistant to a valine analogue, is useful for the production of high levels of L-valine by fermentation.
摘要翻译： 通过将具有与L-缬氨酸生产有关的遗传信息插入其中的衍生自埃希氏杆菌属供体菌株的DNA片段的杂交质粒的并入埃希氏菌属宿主菌株的构建的L-缬氨酸生产微生物 其对缬氨酸类似物具有抗性，可用于通过发酵生产高水平的L-缬氨酸。
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公开(公告)号：US4452890A
公开(公告)日：1984-06-05
申请号：US376396
申请日：1982-05-10
IPC分类号： C12P19/34 , C12N1/21 , C12N15/09 , C12N15/52 , C12N15/77 , C12P13/08 , C12R1/13 , C12R1/15 , C12N15/00
摘要： An L-threonine microorganism is produced by inserting a restriction endonuclease fragment of chromosomal DNA controlling .alpha.-amino-.beta.-hydroxy valeric acid resistance from a Brevibacterium or Corynebacterium into a plasmid and transforming a Brevibacterium or Corynebacterium which is sensitive to .alpha.-amino-.beta.-hydroxy valeric acid.
摘要翻译： 通过将控制α-氨基-β-羟基戊酸抗性的染色体DNA的限制性内切核酸酶片段从短杆菌属或棒状杆菌中插入到质粒中并转化对α-氨基-ββ敏感的短杆菌属或棒状杆菌来产生L-苏氨酸微生物 - 羟基戊酸。
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